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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP856
Library VH
(Link to library)
Clone ID VHP856
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10840-1
Original site URL
Representative seq. ID VHP856P
(Link to Original site)
Representative DNA sequence 
>VHP856 (VHP856Q) /CSM/VH/VHP8-C/VHP856Q.Seq.d/
AGATTTTGTTATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTA
GTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAG
AAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTT
CAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTT
TAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTT
ATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTG
TTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAG
GACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTTAGTTCATT
GGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATTGTTATCAC
CATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAAAGAAGTTAAGGATA
CACAAACXXXXXXXXXXGTTCTTTTAATTCTCTACGCTTTCCATTGTACTTGGGTCACTA
GTGAAGCCTACTCATCTCCATCCATTGTACTCTCTGCCAAACAAAACGATGGTAGTCGTG
TGATTTTCGATGATTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATACTGCCGACGACG
CTCGTATTATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGCCAATCGTACCG
TATTGGTTGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGGTAAAGCATTTG
CATCCACTGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGATTATGTTTTAG
TTATTTTTGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATTCCTTTGGATGG
TTAGAATTGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCTCACCAATGGTC
AATATAGAATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCATGTACAAACTTA
GTTACTATCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGGTTTCGATCGTG
TAAGAAATGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGAAGAAGCTTTCA
CATCTGTTCATTGGTTAGTTAGAGTTTATAAAGTTAAAGATTT
sequence update 2002.10.25
Translated Amino Acid sequence 
illf*sinivciciiivyisv*YISIFKQINNKKKMKRSEKSSTSVVSNNKQQDVNIISS
NEVGVKEENKGHQEFLLKVLILSVIYVLAFSTRLFSVLRYESVIHEFDPYFNYRSTIYLV
QEGFYNFLNWFDERAWYPLGRIVGGTIYPGLMATASLVHWSLNSLNITVNIRNVCVLLSP
WFASNTAMVTYKFAKEVKDTQ---

---VLLILYAFHCTWVTSEAYSSPSIVLSAKQNDGSRVIFDDFREAYRWIGQNTADDARI
MSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYIQMKALDVDYVLVIF
GGLTGYSSDDINKFLWMVRIGGSCDPNIKEQDYLTNGQYRIDKGASPTMLNSLMYKLSYY
RFSEVHTDYQRPTGFDRVRNVEIGNKNFDLTYLEEAFTSVHWLVRVYKVKD
Translated Amino Acid sequence (All Frames) 
Frame A:
rfcyfsqsilcvyv**lcilvyniylflnrstirkk*kdqknqvhlllvitnnkm*issv
qmklvlkkkikdiknsy*kf*fyqsfmf*hfqlvysqyyvmkvlfmnlihiliidqqyil
fkkvfiif*iglmkehgih*dvl*vvqftqv*wqqqv*figh*ih*ilqlilemyvycyh
hglhqiqqw*pinlpkklrihk---

---VLLILYAFHCTWVTSEAYSSPSIVLSAKQNDGSRVIFDDFREAYRWIGQNTADDARI
MSWWDYGYQLSAMANRTVLVDNNTWNNSHIAQVGKAFASTEEDAYIQMKALDVDYVLVIF
GGLTGYSSDDINKFLWMVRIGGSCDPNIKEQDYLTNGQYRIDKGASPTMLNSLMYKLSYY
RFSEVHTDYQRPTGFDRVRNVEIGNKNFDLTYLEEAFTSVHWLVRVYKVKD

Frame B:
dfvilvnqycvymynncvy*ciiyiyf*tdqq*eknekirkikyicc***qttrckyhqf
k*swc*rrk*rtsriliksfnsishlcfsifnsfilsitl*kcys*i*sif*l*innisc
srrfl*ffklv**ksmvsirtycrwynlprfngnskfsslviefieyys*y*kcmcivit
mvcikysngnl*icqrs*gytn---

---ff*fstlsivlgslvkpthlhplyslpnktmvvv*fsmisvkptvglvkilpttlvl
clggimvinylqwpivpywliitlgtivislklvkhlhplkkmltyk*khwmsimf*lfl
vv*lvtvlmisinsfgwlelvvvviqilknkiispmvnie*ikvphqqc*ilsctnlvti
vslkftltikdqqvsiv*emlklvikisi*li*kklshlfig*lefiklki

Frame C:
illf*sinivciciiivyisv*YISIFKQINNKKKMKRSEKSSTSVVSNNKQQDVNIISS
NEVGVKEENKGHQEFLLKVLILSVIYVLAFSTRLFSVLRYESVIHEFDPYFNYRSTIYLV
QEGFYNFLNWFDERAWYPLGRIVGGTIYPGLMATASLVHWSLNSLNITVNIRNVCVLLSP
WFASNTAMVTYKFAKEVKDTQ---

---sfnslrfplylgh**sllisihctlcqtkrw*scdfr*fp*slpldwskycrrrsyy
vlvglwlsiicngqsyrig***hleq*syrssw*sicih*rrclhtnesigcrlcfsyfw
wfnwlqf**yq*ipldg*nww*l*sky*rtrlshqwsi*nr*rcltnnvkfshvqt*lls
fl*ssh*lsktnrfrsckkc*nw**kfrfnlfrrsfhicslvs*sl*s*rf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP856 (VHP856Q) /CSM/VH/VHP8-C/VHP856Q.Seq.d/                       2333   0.0
VHQ247 (VHQ247Q) /CSM/VH/VHQ2-B/VHQ247Q.Seq.d/                       1360   0.0
VHO701 (VHO701Q) /CSM/VH/VHO7-A/VHO701Q.Seq.d/                       1360   0.0
VHJ693 (VHJ693Q) /CSM/VH/VHJ6-D/VHJ693Q.Seq.d/                       1360   0.0
VHJ440 (VHJ440Q) /CSM/VH/VHJ4-B/VHJ440Q.Seq.d/                       1360   0.0
AHN309 (AHN309Q) /CSM/AH/AHN3-A/AHN309Q.Seq.d/                       1360   0.0
AHF418 (AHF418Q) /CSM/AH/AHF4-A/AHF418Q.Seq.d/                       1360   0.0
AHE323 (AHE323Q) /CSM/AH/AHE3-A/AHE323Q.Seq.d/                       1360   0.0
VHI661 (VHI661Q) /CSM/VH/VHI6-C/VHI661Q.Seq.d/                       1354   0.0
VHH861 (VHH861Q) /CSM/VH/VHH8-C/VHH861Q.Seq.d/                       1354   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446657) Dictyostelium discoideum cDNA clone:ddv63m12, 3' ...  1358   0.0    1
(BJ446434) Dictyostelium discoideum cDNA clone:ddv62p14, 3' ...  1358   0.0    1
(BJ445595) Dictyostelium discoideum cDNA clone:ddv60b01, 3' ...  1358   0.0    1
(BJ442751) Dictyostelium discoideum cDNA clone:ddv50i24, 3' ...  1358   0.0    1
(BJ442305) Dictyostelium discoideum cDNA clone:ddv49p10, 3' ...  1358   0.0    1
(BJ355414) Dictyostelium discoideum cDNA clone:dda57b03, 3' ...  1358   0.0    1
(BJ350789) Dictyostelium discoideum cDNA clone:dda41d06, 3' ...  1358   0.0    1
(BJ350232) Dictyostelium discoideum cDNA clone:dda39n05, 3' ...  1358   0.0    1
(BJ442026) Dictyostelium discoideum cDNA clone:ddv48i16, 3' ...  1352   0.0    1
(BJ441411) Dictyostelium discoideum cDNA clone:ddv46j16, 3' ...  1352   0.0    1
dna update 2008.12.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT044907_1(BT044907|pid:none) Salmo salar clone ssal-rgf-507-033...   353   8e-96
L47337_1(L47337|pid:none) Homo sapiens transmembrane protein (TM...   351   4e-95
BC085031_1(BC085031|pid:none) Xenopus laevis similar to intergra...   351   4e-95
AK295892_1(AK295892|pid:none) Homo sapiens cDNA FLJ58575 complet...   350   6e-95
CQ834708_1(CQ834708|pid:none) Sequence 579 from Patent WO2004058...   350   6e-95
AY891653_1(AY891653|pid:none) Synthetic construct Homo sapiens c...   350   6e-95
(P46977) RecName: Full=Dolichyl-diphosphooligosaccharide--protei...   350   6e-95
BC048348_1(BC048348|pid:none) Homo sapiens STT3, subunit of the ...   350   6e-95
BT064584_1(BT064584|pid:none) Zea mays full-length cDNA clone ZM...   349   1e-94
CQ834710_1(CQ834710|pid:none) Sequence 581 from Patent WO2004058...   349   1e-94
protein update 2009. 7.21
PSORT 

 psg: 0.22  gvh: 0.21  alm: 0.22  top: 0.53  tms: 0.07  mit: 0.36  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHP856 is mit
5' end seq. ID VHP856F
5' end seq. 
>VHP856F.Seq
AGATTTTGTTATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTA
GTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAG
AAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTT
CAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTT
TAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTT
ATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTG
TTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAG
GACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTTAGTTCATT
GGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATTGTTATCAC
CATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAAAGAAGTTAAGGATA
CACAAACNNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHP856Z
3' end seq. 
>VHP856Z.Seq
NNNNNNNNNNGTTCTTTTAATTCTCTACGCTTTCCATTGTACTTGGGTCACTAGTGAAGC
CTACTCATCTCCATCCATTGTACTCTCTGCCAAACAAAACGATGGTAGTCGTGTGATTTT
CGATGATTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATACTGCCGACGACGCTCGTAT
TATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGCCAATCGTACCGTATTGGT
TGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGGTAAAGCATTTGCATCCAC
TGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGATTATGTTTTAGTTATTTT
TGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATTCCTTTGGATGGTTAGAAT
TGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCTCACCAATGGTCAATATAG
AATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCATGTACAAACTTAGTTACTA
TCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGGTTTCGATCGTGTAAGAAA
TGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGAAGAAGCTTTCACATCTGT
TCATTGGTTAGTTAGAGTTTATAAAGTTAAAGATTT
Length of 3' end seq. 696
Connected seq. ID VHP856P
Connected seq. 
>VHP856P.Seq
AGATTTTGTTATTTTAGTCAATCAATATTGTGTGTATATGTATAATAATTGTGTATATTA
GTGTATAATATATATCTATTTTTAAACAGATCAACAATAAGAAAAAAATGAAAAGATCAG
AAAAATCAAGTACATCTGTTGTTAGTAATAACAAACAACAAGATGTAAATATCATCAGTT
CAAATGAAGTTGGTGTTAAAGAAGAAAATAAAGGACATCAAGAATTCTTATTAAAAGTTT
TAATTCTATCAGTCATTTATGTTTTAGCATTTTCAACTCGTTTATTCTCAGTATTACGTT
ATGAAAGTGTTATTCATGAATTTGATCCATATTTTAATTATAGATCAACAATATATCTTG
TTCAAGAAGGTTTTTATAATTTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCATTAG
GACGTATTGTAGGTGGTACAATTTACCCAGGTTTAATGGCAACAGCAAGTTTAGTTCATT
GGTCATTGAATTCATTGAATATTACAGTTAATATTAGAAATGTATGTGTATTGTTATCAC
CATGGTTTGCATCAAATACAGCAATGGTAACCTATAAATTTGCCAAAGAAGTTAAGGATA
CACAAAC----------GTTCTTTTAATTCTCTACGCTTTCCATTGTACTTGGGTCACTA
GTGAAGCCTACTCATCTCCATCCATTGTACTCTCTGCCAAACAAAACGATGGTAGTCGTG
TGATTTTCGATGATTTCCGTGAAGCCTACCGTTGGATTGGTCAAAATACTGCCGACGACG
CTCGTATTATGTCTTGGTGGGATTATGGTTATCAATTATCTGCAATGGCCAATCGTACCG
TATTGGTTGATAATAACACTTGGAACAATAGTCATATCGCTCAAGTTGGTAAAGCATTTG
CATCCACTGAAGAAGATGCTTACATACAAATGAAAGCATTGGATGTCGATTATGTTTTAG
TTATTTTTGGTGGTTTAACTGGTTACAGTTCTGATGATATCAATAAATTCCTTTGGATGG
TTAGAATTGGTGGTAGTTGTGATCCAAATATTAAAGAACAAGATTATCTCACCAATGGTC
AATATAGAATAGATAAAGGTGCCTCACCAACAATGTTAAATTCTCTCATGTACAAACTTA
GTTACTATCGTTTCTCTGAAGTTCACACTGACTATCAAAGACCAACAGGTTTCGATCGTG
TAAGAAATGTTGAAATTGGTAATAAAAATTTCGATTTAACTTATTTAGAAGAAGCTTTCA
CATCTGTTCATTGGTTAGTTAGAGTTTATAAAGTTAAAGATTT
Length of connected seq. 1293
Full length Seq ID -
Full length Seq. -
Length of full length seq. -