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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP820
Library VH
(Link to library)
Clone ID VHP820
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16379-1
Original site URL
Representative seq. ID VHP820P
(Link to Original site)
Representative DNA sequence 
>VHP820 (VHP820Q) /CSM/VH/VHP8-A/VHP820Q.Seq.d/
ATTATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTT
GAAAAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGAT
AATATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTC
AGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATXXXXXXX
XXXTTCGAGCCAACCAAAGGTGAACCATCTTCAGATACNTTGTTAAGAGATAAAGTAAAC
ACTAAATTGGGTATGTTGGGTGATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAA
GCTTTCAAAGCAGACTCTTCAAGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACA
ATCGTAAAGAATGGTGGTGAAGCTGATCAACAAATCATCATTGATCAATATCTTAAATCT
TCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAA
TTGGTTGAAAAAGCCCTCAATTTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTAT
ATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAA
AACTTTAATCAAATCAACGAAACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGT
AGTTGTCTCACCTCTAAAATGGATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAA
GATCATCCAGTTCCAATTGCTGATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATT
AATTCAAATTGGTTCAAGGCTTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAA
AAATAAAATCTCTTACAAGGAAAGATCTTTGTTTTAATAATNATAGNATAAAAAAAAATA
GACGAAAACTAAAATAAACATCAAATTAT
sequence update 2002.10.25
Translated Amino Acid sequence 
IIFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVF
SGKVDITINIVKPTKKI---

---FEPTKGEPSSDTLLRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVL
ATIVKNGGEADQQIIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQD
SYMVFFTLPNRVRQFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNF
FKDHPVPIADRSIKQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllqgkifvlixix*k
kiden*nkhqi
Translated Amino Acid sequence (All Frames) 
Frame A:
IIFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVF
SGKVDITINIVKPTKKI---

---FEPTKGEPSSDTLLRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVL
ATIVKNGGEADQQIIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQD
SYMVFFTLPNRVRQFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNF
FKDHPVPIADRSIKQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllqgkifvlixix*k
kiden*nkhqi

Frame B:
lfl*fhlfililyi*iii*lkkcviini*ltk*id*yyqiisyqvntiyildqi*rilys
aakltlqsillnqpkks---

---ssqpkvnhlqixc*eik*tlnwvcwvmlisllnvrkdsklskqtlqvyhqifvlqyl
lqs*rmvvklinksslinilnll*slkriltfwyllwlqkrnwlkkpsislylhplevki
viwfslpsqivfvnslgltspktlikstkhlktllclvvslvvvsplkwmilntmksstf
skiiqfqllivqlnki*kwlqliqigsrllikiyqigfnqnknkisykerslf**x*xkk
k*tktkinikl

Frame C:
yfynftclf*fytyk**ys*knv*s*tfs*qsr*isitr*yrtk*irfts*tkfkgfciq
rqs*hynqyc*tnqknh---

---ranqr*tifryxvkr*skh*igyvg*c*yrc*m*ekirsfqsrlfkftirysfystc
ynrkeww*s*stnhh*sis*iffsr*keflpfgtcfgskrgig*kspqfrfisir*ksr*
lygflyppkscssirlglphqkl*snqrni*klsfvwsyhw*lshl*ng*fsiq*srqlf
qrssssnc*sfn*trfrngyn*fklvqgf*srfikldsikikiksltrkdlcfnnxxikk
nrrklk*tsny
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP820 (VHP820Q) /CSM/VH/VHP8-A/VHP820Q.Seq.d/                       1407   0.0
VHO659 (VHO659Q) /CSM/VH/VHO6-C/VHO659Q.Seq.d/                       1392   0.0
VHB785 (VHB785Q) /CSM/VH/VHB7-D/VHB785Q.Seq.d/                       1392   0.0
AHD767 (AHD767Q) /CSM/AH/AHD7-C/AHD767Q.Seq.d/                       1392   0.0
VHP541 (VHP541Q) /CSM/VH/VHP5-B/VHP541Q.Seq.d/                       1390   0.0
VHN747 (VHN747Q) /CSM/VH/VHN7-B/VHN747Q.Seq.d/                       1386   0.0
AHF665 (AHF665Q) /CSM/AH/AHF6-C/AHF665Q.Seq.d/                       1386   0.0
AHE191 (AHE191Q) /CSM/AH/AHE1-D/AHE191Q.Seq.d/                       1386   0.0
VHJ445 (VHJ445Q) /CSM/VH/VHJ4-B/VHJ445Q.Seq.d/                       1384   0.0
VHB329 (VHB329Q) /CSM/VH/VHB3-B/VHB329Q.Seq.d/                       1384   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446239) Dictyostelium discoideum cDNA clone:ddv62h06, 3' ...  1382   0.0    2
(BJ446279) Dictyostelium discoideum cDNA clone:ddv62a11, 3' ...  1366   0.0    2
(BJ445758) Dictyostelium discoideum cDNA clone:ddv60e16, 3' ...  1366   0.0    2
(BJ445099) Dictyostelium discoideum cDNA clone:ddv58n11, 3' ...  1366   0.0    2
(BJ444540) Dictyostelium discoideum cDNA clone:ddv56o16, 3' ...  1366   0.0    1
(BJ444381) Dictyostelium discoideum cDNA clone:ddv56o05, 3' ...  1366   0.0    1
(BJ444327) Dictyostelium discoideum cDNA clone:ddv56c04, 3' ...  1366   0.0    1
(BJ442274) Dictyostelium discoideum cDNA clone:ddv49j12, 3' ...  1366   0.0    2
(BJ441929) Dictyostelium discoideum cDNA clone:ddv48e09, 3' ...  1366   0.0    2
(BJ437633) Dictyostelium discoideum cDNA clone:ddv34j21, 3' ...  1366   0.0    2
dna update 2008.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   230   6e-59
(Q11011) RecName: Full=Puromycin-sensitive aminopeptidase;      ...   111   4e-23
BC098978_1(BC098978|pid:none) Xenopus laevis hypothetical protei...   111   4e-23
EU686614_22(EU686614|pid:none) Uncultured marine crenarchaeote K...   110   6e-23
AY550051_1(AY550051|pid:none) Sus scrofa puromycin sensitive ami...   107   6e-22
BC124025_1(BC124025|pid:none) Xenopus tropicalis aminopeptidase ...   107   8e-22
A65888_1(A65888|pid:none) Sequence 1 from Patent WO9738114.  &Y0...   106   1e-21
AK296887_1(AK296887|pid:none) Homo sapiens cDNA FLJ53354 complet...   106   1e-21
AB220536_1(AB220536|pid:none) Macaca fascicularis mRNA, clone Qo...   106   1e-21
(P55786) RecName: Full=Puromycin-sensitive aminopeptidase;      ...   106   1e-21
protein update 2009. 7.21
PSORT 

 psg: 0.98  gvh: 0.36  alm: 0.35  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for VHP820 is cyt
5' end seq. ID VHP820F
5' end seq. 
>VHP820F.Seq
ATTATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTT
GAAAAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGAT
AATATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTC
AGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATNNNNNNN
NNN
Length of 5' end seq. 243
3' end seq. ID VHP820Z
3' end seq. 
>VHP820Z.Seq
NNNNNNNNNNTTCGAGCCAACCAAAGGTGAACCATCTTCAGATACNTTGTTAAGAGATAA
AGTAAACACTAAATTGGGTATGTTGGGTGATGCTGATATCGTTGCTGAATGTAAGAAAAG
ATTCGAAGCTTTCAAAGCAGACTCTTCAAGTTTACCATCAGATATTCGTTCTACAGTACT
TGCTACAATCGTAAAGAATGGTGGTGAAGCTGATCAACAAATCATCATTGATCAATATCT
TAAATCTTCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGTACTTGCTTTGGCTCCAAA
AGAGGAATTGGTTGAAAAAGCCCTCAATTTCGCTTTATCTCCATCCGTTAGAAGTCAAGA
TAGTTATATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCAATTCGCTTGGGCTTACCT
CACCAAAAACTTTAATCAAATCAACGAAACATTTAAAAACTCTCCTTTGTTTGGTCGTAT
CATTGGTAGTTGTCTCACCTCTAAAATGGATGATTCTCAATACAATGAAGTCGTCAACTT
TTTCAAAGATCATCCAGTTCCAATTGCTGATCGTTCAATTAAACAAGATTTAGAAATGGT
TACAATTAATTCAAATTGGTTCAAGGCTTTTAATCAAGATTTATCAAATTGGATTCAATC
AAAATAAAAATAAAATCTCTTACAAGGAAAGATCTTTGTTTTAATAATNATAGNATAAAA
AAAAATAGACGAAAACTAAAATAAACATCAAATTAT
Length of 3' end seq. 756
Connected seq. ID VHP820P
Connected seq. 
>VHP820P.Seq
ATTATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTT
GAAAAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCAGAT
AATATCGTACCAAGTAAATACGATTTACATCTTAGACCAAATTTAAAGGATTTTGTATTC
AGCGGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCAT-------
---TTCGAGCCAACCAAAGGTGAACCATCTTCAGATACNTTGTTAAGAGATAAAGTAAAC
ACTAAATTGGGTATGTTGGGTGATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAA
GCTTTCAAAGCAGACTCTTCAAGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACA
ATCGTAAAGAATGGTGGTGAAGCTGATCAACAAATCATCATTGATCAATATCTTAAATCT
TCTTTAGTCGCTGAAAAGAATTCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAA
TTGGTTGAAAAAGCCCTCAATTTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTAT
ATGGTTTTCTTTACCCTCCCAAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAA
AACTTTAATCAAATCAACGAAACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGT
AGTTGTCTCACCTCTAAAATGGATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAA
GATCATCCAGTTCCAATTGCTGATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATT
AATTCAAATTGGTTCAAGGCTTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAA
AAATAAAATCTCTTACAAGGAAAGATCTTTGTTTTAATAATNATAGNATAAAAAAAAATA
GACGAAAACTAAAATAAACATCAAATTAT
Length of connected seq. 979
Full length Seq ID -
Full length Seq. -
Length of full length seq. -