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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP768
Library VH
(Link to library)
Clone ID VHP768
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15732-1
Original site URL
Representative seq. ID VHP768P
(Link to Original site)
Representative DNA sequence 
>VHP768 (VHP768Q) /CSM/VH/VHP7-C/VHP768Q.Seq.d/
GTAAAACCATTATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAA
CTCAACCAATCAAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAG
CCAAAGAGGCAGAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAG
CAGCAGCAAAAGAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAA
AAGAAGCTGAAGAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTG
AAAAAATTTTAAAAACCCCAAAGGGTGAAAAGAAAGATGTTTCAAGTTTATTATCATCAT
ATCATCCAACAGCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTT
CACCAGAGAAACAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTG
TTATTCCACCACCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAA
TTCAAGATGCAGTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAG
GTACAGATCATGCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAGX
XXXXXXXXXGATGAATTAATCGCTAAACTCTATGAAGGTAATTTAGATTCTAAAGAAATT
GAAAAAGCAACCTCTGGTGTTAAATCCGATTTCCCAACTGGTATTGCAGAGTGTGGTACT
GATGCAATGAGATTTGCCCTTTGTGCTTACACTTCACAAGGTCGTGATATCAATTTAGAT
ATTCAACGTGTCGTTGGTTATCGTAATTTCTGTAATAAGATTTGGAATGCCACTAGATTC
GCTCATATGAAATTGGGTGATTCTTTCAAACCGGAATCATTTGATGCCTCAAAACTTTTA
CAACAATCAAATGCTATCAATCTTTGGATTCTTAACGCTGCTCAACGTGCTATCACCCTC
TCTGAAGAAGGTTTCAAAGAGTACGATTTTAGTAAGGTTACCACTGCTATCTATAGTTTC
TGGTTGAATGAAATGTGTGATGTTTATCTCGAAATGACCAAATCCATTTTCTCACTCGAA
GAAGATTCACCAATTAAACAAAAAACCAAAGAAACCCTTTACACTTGTATCGATATTGGT
CTTCGTTTATTACATCCATTCATGCCATACCTCACCGAGGAGTTGTATCAATCATTACCA
CGTCGTCCAGAAGATGTCATTCCAACCATTATGTTATGTCCATACCCACAATCTCAATCA
TGTTGGGCCAATTCTGACATTGAGGAAGAAAGAAACAATGTCAAGATATCATCAAATCAA
TTCGTTCACTTCGTGCTACCTATACCA
sequence update 2002.10.25
Translated Amino Acid sequence 
ktiiekdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQA
AAKEKELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKKDVSSLLSSY
HPTAVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSI
QDAVVRYHRMKGEVCLWVPGTDHAGIATQVVVEKKIWKE---

---DELIAKLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFALCAYTSQGRDINLD
IQRVVGYRNFCNKIWNATRFAHMKLGDSFKPESFDASKLLQQSNAINLWILNAAQRAITL
SEEGFKEYDFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLEEDSPIKQKTKETLYTCIDIG
LRLLHPFMPYLTEELYQSLPRRPEDVIPTIMLCPYPQSQSCWANSDIEEERNNVKISSNQ
FVHFVLPIP
Translated Amino Acid sequence (All Frames) 
Frame A:
vkpllkkt*iynk*cqnqnqlnqskrlmrklkermkrkrkpkrqrkqrn*qk*kkrklnk
qqqkkknykinlkknkrkkkkklkkkknkklkkkllenilkkf*kpqrvkrkmfqvyyhh
iiqqqlnqfgmiig*imdifhqrnkwrfnhm*lkiksl*llfhhqmlqvvyi*dmh*piq
fkmqlfviiv*rvkfvygyqvqimqvlqhk*l*rkrfgk---

---DELIAKLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFALCAYTSQGRDINLD
IQRVVGYRNFCNKIWNATRFAHMKLGDSFKPESFDASKLLQQSNAINLWILNAAQRAITL
SEEGFKEYDFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLEEDSPIKQKTKETLYTCIDIG
LRLLHPFMPYLTEELYQSLPRRPEDVIPTIMLCPYPQSQSCWANSDIEEERNNVKISSNQ
FVHFVLPIP

Frame B:
*nhy*krhkyiinnvrtktnstnqrd**gn*kke*rekesqrgresreisknerkgs*ts
sskrkritk*t*krtrekrkrs*rkrktrs*rknc*rty*knfknpkg*kercfkfiiii
ssnss*inlv*llvr*wifftretngdsttcs*r*kvcncysttkcyr*ftfrtcinqfn
srcscslssyeg*slfmgtryrscrycntsscrekdler---

---mn*slnsmkvi*ilkklkkqplvlnpisqlvlqsvvlmq*dlpfvltlhkvvisi*i
fnvslvivisvirfgmpldsli*nwvilsnrnhlmpqnfynnqmlsifgfltllnvlsps
lkkvskstilvrlpllsivsg*mkcvmfisk*pnpfshskkihqlnkkpkkpftlvsilv
fvyyihschtsprscinhyhvvqkmsfqplcyvhthnlnhvgpiltlrkketmsryhqin
sftscyly

Frame C:
ktiiekdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQA
AAKEKELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKKDVSSLLSSY
HPTAVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSI
QDAVVRYHRMKGEVCLWVPGTDHAGIATQVVVEKKIWKE---

---*inr*tl*r*frf*rn*ksnlwc*irfpnwycrvwy*cneicplclhftrs*yqfry
stcrwls*fl**dlech*irsyeig*ffqtgii*clktfttikcyqslds*rcstcyhpl
*rrfqrvrf**gyhcyl*flve*nv*clsrndqihfltrrrftn*tknqrnplhlyryws
sfitsihaiphrgvvsiittssrrchsnhyvmsiptisimlgqf*h*grkkqcqdiiksi
rslratyt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP768 (VHP768Q) /CSM/VH/VHP7-C/VHP768Q.Seq.d/                       2105   0.0
VHJ511 (VHJ511Q) /CSM/VH/VHJ5-A/VHJ511Q.Seq.d/                       1294   0.0
VHI423 (VHI423Q) /CSM/VH/VHI4-A/VHI423Q.Seq.d/                        791   0.0
VHM840 (VHM840Q) /CSM/VH/VHM8-B/VHM840Q.Seq.d/                        682   0.0
VHL308 (VHL308Q) /CSM/VH/VHL3-A/VHL308Q.Seq.d/                        682   0.0
SHI109 (SHI109Q) /CSM/SH/SHI1-A/SHI109Q.Seq.d/                        682   0.0
CHO422 (CHO422Q) /CSM/CH/CHO4-A/CHO422Q.Seq.d/                        682   0.0
VHP692 (VHP692Q) /CSM/VH/VHP6-D/VHP692Q.Seq.d/                        674   0.0
VHA717 (VHA717Q) /CSM/VH/VHA7-A/VHA717Q.Seq.d/                        674   0.0
SHA541 (SHA541Q) /CSM/SH/SHA5-B/SHA541Q.Seq.d/                        644   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446399) Dictyostelium discoideum cDNA clone:ddv62h17, 3' ...  1461   0.0    1
(AC115612) Dictyostelium discoideum chromosome 2 map 6245135...  1350   0.0    2
(BJ442491) Dictyostelium discoideum cDNA clone:ddv50e03, 3' ...  1197   0.0    2
(BJ441588) Dictyostelium discoideum cDNA clone:ddv47n06, 3' ...   579   0.0    3
(BJ427490) Dictyostelium discoideum cDNA clone:ddv62h17, 5' ...   636   0.0    2
(BJ395739) Dictyostelium discoideum cDNA clone:dds40a03, 5' ...   636   0.0    2
(BJ418208) Dictyostelium discoideum cDNA clone:ddv32b05, 5' ...   636   0.0    2
(BJ391796) Dictyostelium discoideum cDNA clone:dds25a11, 5' ...   601   0.0    2
(BJ369333) Dictyostelium discoideum cDNA clone:ddc50l06, 5' ...   636   0.0    2
(BJ370138) Dictyostelium discoideum cDNA clone:ddc53e03, 5' ...   597   0.0    2
dna update 2008.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KU2) RecName: Full=Probable valyl-tRNA synthetase, cytoplasm...   466   e-135
BC090426_1(BC090426|pid:none) Danio rerio valyl-tRNA synthetase,...   254   7e-66
BC084762_1(BC084762|pid:none) Xenopus laevis hypothetical LOC495...   251   1e-65
AF506220_1(AF506220|pid:none) Danio rerio valyl-tRNA synthetase ...   244   5e-63
AK167842_1(AK167842|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...   240   8e-62
BC053703_1(BC053703|pid:none) Mus musculus valyl-tRNA synthetase...   240   8e-62
(Q9Z1Q9) RecName: Full=Valyl-tRNA synthetase;          EC=6.1.1....   240   8e-62
AF087141_1(AF087141|pid:none) Mus musculus uncharacterized long ...   238   3e-61
(Q04462) RecName: Full=Valyl-tRNA synthetase;          EC=6.1.1....   238   3e-61
AB172992_1(AB172992|pid:none) Macaca fascicularis brain cDNA clo...   238   4e-61
protein update 2009. 7.21
PSORT 

 psg: 0.61  gvh: 0.07  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VHP768 is nuc
5' end seq. ID VHP768F
5' end seq. 
>VHP768F.Seq
GTAAAACCATTATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAA
CTCAACCAATCAAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAG
CCAAAGAGGCAGAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAG
CAGCAGCAAAAGAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAA
AAGAAGCTGAAGAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTG
AAAAAATTTTAAAAACCCCAAAGGGTGAAAAGAAAGATGTTTCAAGTTTATTATCATCAT
ATCATCCAACAGCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTT
CACCAGAGAAACAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTG
TTATTCCACCACCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAA
TTCAAGATGCAGTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAG
GTACAGATCATGCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAGN
NNNNNNNNN
Length of 5' end seq. 669
3' end seq. ID VHP768Z
3' end seq. 
>VHP768Z.Seq
NNNNNNNNNNGATGAATTAATCGCTAAACTCTATGAAGGTAATTTAGATTCTAAAGAAAT
TGAAAAAGCAACCTCTGGTGTTAAATCCGATTTCCCAACTGGTATTGCAGAGTGTGGTAC
TGATGCAATGAGATTTGCCCTTTGTGCTTACACTTCACAAGGTCGTGATATCAATTTAGA
TATTCAACGTGTCGTTGGTTATCGTAATTTCTGTAATAAGATTTGGAATGCCACTAGATT
CGCTCATATGAAATTGGGTGATTCTTTCAAACCGGAATCATTTGATGCCTCAAAACTTTT
ACAACAATCAAATGCTATCAATCTTTGGATTCTTAACGCTGCTCAACGTGCTATCACCCT
CTCTGAAGAAGGTTTCAAAGAGTACGATTTTAGTAAGGTTACCACTGCTATCTATAGTTT
CTGGTTGAATGAAATGTGTGATGTTTATCTCGAAATGACCAAATCCATTTTCTCACTCGA
AGAAGATTCACCAATTAAACAAAAAACCAAAGAAACCCTTTACACTTGTATCGATATTGG
TCTTCGTTTATTACATCCATTCATGCCATACCTCACCGAGGAGTTGTATCAATCATTACC
ACGTCGTCCAGAAGATGTCATTCCAACCATTATGTTATGTCCATACCCACAATCTCAATC
ATGTTGGGCCAATTCTGACATTGAGGAAGAAAGAAACAATGTCAAGATATCATCAAATCA
ATTCGTTCACTTCGTGCTACCTATACCA
Length of 3' end seq. 748
Connected seq. ID VHP768P
Connected seq. 
>VHP768P.Seq
GTAAAACCATTATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAA
CTCAACCAATCAAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAG
CCAAAGAGGCAGAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAG
CAGCAGCAAAAGAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAA
AAGAAGCTGAAGAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTG
AAAAAATTTTAAAAACCCCAAAGGGTGAAAAGAAAGATGTTTCAAGTTTATTATCATCAT
ATCATCCAACAGCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTT
CACCAGAGAAACAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTG
TTATTCCACCACCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAA
TTCAAGATGCAGTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAG
GTACAGATCATGCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAG-
---------GATGAATTAATCGCTAAACTCTATGAAGGTAATTTAGATTCTAAAGAAATT
GAAAAAGCAACCTCTGGTGTTAAATCCGATTTCCCAACTGGTATTGCAGAGTGTGGTACT
GATGCAATGAGATTTGCCCTTTGTGCTTACACTTCACAAGGTCGTGATATCAATTTAGAT
ATTCAACGTGTCGTTGGTTATCGTAATTTCTGTAATAAGATTTGGAATGCCACTAGATTC
GCTCATATGAAATTGGGTGATTCTTTCAAACCGGAATCATTTGATGCCTCAAAACTTTTA
CAACAATCAAATGCTATCAATCTTTGGATTCTTAACGCTGCTCAACGTGCTATCACCCTC
TCTGAAGAAGGTTTCAAAGAGTACGATTTTAGTAAGGTTACCACTGCTATCTATAGTTTC
TGGTTGAATGAAATGTGTGATGTTTATCTCGAAATGACCAAATCCATTTTCTCACTCGAA
GAAGATTCACCAATTAAACAAAAAACCAAAGAAACCCTTTACACTTGTATCGATATTGGT
CTTCGTTTATTACATCCATTCATGCCATACCTCACCGAGGAGTTGTATCAATCATTACCA
CGTCGTCCAGAAGATGTCATTCCAACCATTATGTTATGTCCATACCCACAATCTCAATCA
TGTTGGGCCAATTCTGACATTGAGGAAGAAAGAAACAATGTCAAGATATCATCAAATCAA
TTCGTTCACTTCGTGCTACCTATACCA
Length of connected seq. 1397
Full length Seq ID -
Full length Seq. -
Length of full length seq. -