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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO852
Library AF
(Link to library)
Clone ID AFO852
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16397-1
Original site URL
Representative seq. ID AFO852Z
(Link to Original site)
Representative DNA sequence 
>AFO852 (AFO852Q) /CSM/AF/AFO8-C/AFO852Q.Seq.d/
XXXXXXXXXXGCTCATGCATTAAAAACTAAAAAATTGGTTTCACTATCTGAACAAAATTT
TAGTTGATTGCTCTGGTCCAGAAGAAAACTTTGGTTGTGATGGTGGTTTAATGAATAATG
CATTTGATTATATCATTAAAAACAAGGGTATTGACACTGAAAGTAGTTATCCATATACTG
CTGAAACTGGTTCAACATGTTTATTCAATAAATCTGATATTGGTGCAACCATTAAAGGTT
ATGTTAACATTACTGCCGGTTCAGAGATTTCATTAGAGAATGGTGCTCAACATGGTCCAG
TATCTGTTGCAATTGATGCTTCCCATAATAGTTTCCAACTCTATACATCTGGTATCTATT
ATGAGCCAAAATGTTCTCCAACTGAATTGGATCATGGTGTTTTAGTTGTTGGTTATGGTG
TTCAAGGTAAAGATGATGAAGGTCCAGTATTAAACAGAAAACAAACCATTGTTATCCACA
AGAATGAAGATAATAAAGTTGAATCCTCTGATGATTCATCTGATAGTGTTAGACCAAAGG
CAAATAATTATTGGATCGTTAAAAATTCATGGGGTACCTCATGGGGTATAAAAGGTTACA
TTTTAATGTCAAAGGATAGAAAGAATAATTGTGGTATTGCATCAGTTAGCTCTTATCCAC
TTGCCTAGATTTATGGTTCCAAAATGAATAGGAGAATGTTTGGATCTATCTAGTAACCTG
AAATGTTTTTCNATAAAAAAATAAAAATAAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---lmh*KLKNWFHYLNKILVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDTESSYPYTA
ETGSTCLFNKSDIGATIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQLYTSGIYY
EPKCSPTELDHGVLVVGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDSSDSVRPKA
NNYWIVKNSWGTSWGIKGYILMSKDRKNNCGIASVSSYPLA*iygskmnrrmfgsi**pe
mffxkkikik
Translated Amino Acid sequence (All Frames) 
Frame A:
---lmh*KLKNWFHYLNKILVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDTESSYPYTA
ETGSTCLFNKSDIGATIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQLYTSGIYY
EPKCSPTELDHGVLVVGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDSSDSVRPKA
NNYWIVKNSWGTSWGIKGYILMSKDRKNNCGIASVSSYPLA*iygskmnrrmfgsi**pe
mffxkkikik


Frame B:
---scikn*kigfti*tkf*lialvqkktlvvmvv**imhliislktrvltlkvvihill
klvqhvysinlilvqplkvmltllpvqrfh*rmvlnmvqyllqlmlpiivsnsihlvsim
sqnvlqlnwimvf*llvmvfkvkmmkvqy*tenkpllstrmkiiklnplmihlivldqrq
iiigslkihgvphgv*kvtf*cqrieriivvlhqlalihlprfmvpk*igecldlssnlk
cfsikk*k*k


Frame C:
---halktkklvslseqnfs*llwsrrklwl*wwfne*ci*lyh*kqgy*h*k*lsiyc*
nwfnmfiq*i*ywcnh*rlc*hycrfrdfirewcstwssiccn*cfp**fptlyiwyll*
akmfsn*igswcfscwlwcsr*r**rssikqktnhcypqe*r**s*il**fi**c*tkgk
*lldr*kfmgylmgykrlhfnvkg*ke*lwycis*llstcldlwfqne*envwiylvt*n
vfx*knknkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO852 (AFO852Q) /CSM/AF/AFO8-C/AFO852Q.Seq.d/                       1382   0.0
SSJ524 (SSJ524Q) /CSM/SS/SSJ5-A/SSJ524Q.Seq.d/                       1356   0.0
SSF462 (SSF462Q) /CSM/SS/SSF4-C/SSF462Q.Seq.d/                       1352   0.0
CFK280 (CFK280Q) /CSM/CF/CFK2-D/CFK280Q.Seq.d/                       1344   0.0
AFO753 (AFO753Q) /CSM/AF/AFO7-C/AFO753Q.Seq.d/                       1344   0.0
AFK758 (AFK758Q) /CSM/AF/AFK7-C/AFK758Q.Seq.d/                       1320   0.0
AFO827 (AFO827Q) /CSM/AF/AFO8-B/AFO827Q.Seq.d/                       1312   0.0
AFM502 (AFM502Q) /CSM/AF/AFM5-A/AFM502Q.Seq.d/                       1312   0.0
SFK650 (SFK650Q) /CSM/SF/SFK6-C/SFK650Q.Seq.d/                       1310   0.0
AFK761 (AFK761Q) /CSM/AF/AFK7-C/AFK761Q.Seq.d/                       1298   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.  1334  0.0  2
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.   961  0.0  5
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.   595  0.0  2
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.    88  5e-53  7
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.    76  3e-51  6
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    88  6e-37  6
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    82  4e-15  2
AF426414|AF426414.1 Delia radicum cathepsin L-like cysteine protease precursor (CP1) mRNA, complete cds.    56  3e-12  3
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.    46  3e-11  4
BQ834906|BQ834906.1 Po_ad_04H08_TEXF1 Psoroptes ovis mixed Psoroptes ovis cDNA clone Po_ad_04H08 5' similar to O46030 CYSTEINE PROTEINASE. Sitophilus zeamais (maize weevil), mRNA sequence.    50  2e-06  3
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   403   e-114
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   216   6e-55
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   216   6e-55
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...   201   4e-52
BC075887_1(BC075887|pid:none) Danio rerio cathepsin L.1, mRNA (c...   205   1e-51
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   200   3e-51
AY554271_1(AY554271|pid:none) Heterodera glycines cathepsin L-li...   199   1e-49
DQ000609_1(DQ000609|pid:none) Globodera pallida cathepsin L-like...   195   2e-49
AY999065_1(AY999065|pid:none) Globodera pallida cathepsin L-like...   195   2e-49
AY336798_1(AY336798|pid:none) Rhipicephalus haemaphysaloides hae...   192   2e-49
protein update 2009. 4.26
PSORT 

 psg: 0.78  gvh: 0.31  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFO852 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFO852Z
3' end seq. 
>AFO852Z.Seq
----------GCTCATGCATTAAAAACTAAAAAATTGGTTTCACTATCTGAACAAAATTT
TAGTTGATTGCTCTGGTCCAGAAGAAAACTTTGGTTGTGATGGTGGTTTAATGAATAATG
CATTTGATTATATCATTAAAAACAAGGGTATTGACACTGAAAGTAGTTATCCATATACTG
CTGAAACTGGTTCAACATGTTTATTCAATAAATCTGATATTGGTGCAACCATTAAAGGTT
ATGTTAACATTACTGCCGGTTCAGAGATTTCATTAGAGAATGGTGCTCAACATGGTCCAG
TATCTGTTGCAATTGATGCTTCCCATAATAGTTTCCAACTCTATACATCTGGTATCTATT
ATGAGCCAAAATGTTCTCCAACTGAATTGGATCATGGTGTTTTAGTTGTTGGTTATGGTG
TTCAAGGTAAAGATGATGAAGGTCCAGTATTAAACAGAAAACAAACCATTGTTATCCACA
AGAATGAAGATAATAAAGTTGAATCCTCTGATGATTCATCTGATAGTGTTAGACCAAAGG
CAAATAATTATTGGATCGTTAAAAATTCATGGGGTACCTCATGGGGTATAAAAGGTTACA
TTTTAATGTCAAAGGATAGAAAGAATAATTGTGGTATTGCATCAGTTAGCTCTTATCCAC
TTGCCTAGATTTATGGTTCCAAAATGAATAGGAGAATGTTTGGATCTATCTAGTAACCTG
AAATGTTTTTCNATAAAAAAATAAAAATAAAAAT
Length of 3' end seq. 744
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -