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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP646
Library VH
(Link to library)
Clone ID VHP646
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHP646P
(Link to Original site)
Representative DNA sequence 
>VHP646 (VHP646Q) /CSM/VH/VHP6-B/VHP646Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAXXXXXXXXXXCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GATTNTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATNTTTATGATGTCACCCTCCACACTGATGCCATCCACANA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGNTGAATTAACTGCC
TCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATT
GGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGT
ATCGGTTCCCCATTATTNTCTGTCAAAGNTCATTTACCAGTATTAGAATCCTTCGGTTTC
ACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCAT
TGGGCTTCAATTGGTGTTGTCAACAAANATAANAAAGCCACTGAAGTCGCTTTAGCCACC
AGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTC
TAAACAATTTAAGTCGCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTT
TAAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---VNVTKGVQYLNEIKDXFVGAFQWATKEGVVCDENMRGIRFNXYDVTLHTDAIHXGGG
QIIPTARRVLYAXELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIVIGEERRIGS
PLXSVKXHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXXKATEVALATRXR
KGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---rqchqrcsife*n*rxfrwcfpmghqrrccl**kherypfqxl*chpph*chpxrww
snhpncssctxcx*inclpnxxrtnxlsrnycprkrywwyllctqssxrycyw*rtsyrf
piixcqxsftsirilrfhr*ftfphcrssfptmcxrslgfnwccqqx*xsh*srfshqkx
krfsprnprs*qiprktlnnlsrxlsfqk**snlkkilkkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---VNVTKGVQYLNEIKDXFVGAFQWATKEGVVCDENMRGIRFNXYDVTLHTDAIHXGGG
QIIPTARRVLYAXELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIVIGEERRIGS
PLXSVKXHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKXXKATEVALATRXR
KGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---smspkvfni*mklkixslvlsngppkkvlfvmkt*evsvsxfmmspstlmpstxvvv
kssqllvvyxmlxn*lppqlx*nqxt**kllpqktllvvftlysivvxvlllvknvvsvp
hyxlskxiyqy*npsvspliyvptlpvklshnvxsiiglqlvlstxixkplksl*ppexe
kv*pqksqiltnstknskqfksxfefskivikskknfkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1164   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1164   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1164   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1164   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1164   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1164   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1164   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1164   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1164   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1164   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1164   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1164   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1164   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1164   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1164   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1164   0.0    1
(BJ427858) Dictyostelium discoideum cDNA clone:ddv63h20, 5' ...  1164   0.0    1
(BJ427848) Dictyostelium discoideum cDNA clone:ddv63f21, 5' ...  1164   0.0    1
(BJ427832) Dictyostelium discoideum cDNA clone:ddv63b20, 5' ...  1164   0.0    1
dna update 2008.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   8e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   2e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   256   9e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   9e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   2e-66
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   254   6e-66
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   253   1e-65
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   253   1e-65
protein update 2009. 7.21
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHP646 is nuc
5' end seq. ID VHP646F
5' end seq. 
>VHP646F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHP646Z
3' end seq. 
>VHP646Z.Seq
NNNNNNNNNNCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTNTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATNTTTATGATGTCACCCTCCACACTGATGCCATCCACANAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGNTGAATTAACTGCCTCCCCAA
CTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTNTCTGTCAAAGNTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAANATAANAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAA
AAAAA
Length of 3' end seq. 665
Connected seq. ID VHP646P
Connected seq. 
>VHP646P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGA----------CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAA
GATTNTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAAC
ATGAGAGGTATCCGTTTCAATNTTTATGATGTCACCCTCCACACTGATGCCATCCACANA
GGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGNTGAATTAACTGCC
TCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATT
GGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGT
ATCGGTTCCCCATTATTNTCTGTCAAAGNTCATTTACCAGTATTAGAATCCTTCGGTTTC
ACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCAT
TGGGCTTCAATTGGTGTTGTCAACAAANATAANAAAGCCACTGAAGTCGCTTTAGCCACC
AGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTC
TAAACAATTTAAGTCGCNCTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTT
TAAAAAAAAAAA
Length of connected seq. 1262
Full length Seq ID -
Full length Seq. -
Length of full length seq. -