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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP624
Library VH
(Link to library)
Clone ID VHP624
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15307-1
Original site URL
Representative seq. ID VHP624P
(Link to Original site)
Representative DNA sequence 
>VHP624 (VHP624Q) /CSM/VH/VHP6-A/VHP624Q.Seq.d/
CAAATGAAACTAGAGGTTTAAGCATGGATGCAGTTGCCAAAGCTGCCTNTGGTCATTTAG
GTTTACCACTTGGTTCTGCTGAAATTGGNGCTGCATTATTTGGTAATTCATTGATCTATA
ACCCAAAANATACAAGANGGTTAAATAGANATTATTTTGTNTTAAGNGCAGGTCATGGTT
CAATGTTTTTATATAGTTGGTTACATTTATCAGGATATGATGTTTCAATTGAANATNTTA
AAAATTTCANACAATTAAATTCAAANACNCCAGGTCATCCAAAATTTCATGACNCACCAG
GCGTTGAAGCTACCACTGGTCCATTAGGTCAAGGTATTGCAAATGCCGTTGGTATTGCAT
CACCATGTAAAATGGCANCAGGTAAATTCAATACTGAACAACATCAAATNTTTAATCAAA
AGGTAGTAGTTTTAGTTGGXXXXXXXXXXATTCTCAGACTATCTTCGTCCAGCCATCCGT
TTGGCTGCTCTCTCACATTTACCAGTAGTTTACATTTTCACTCATGACTCTGTTGGTGTT
GGTGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCTTCGTATGATTCCA
AACTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGCTTTCTCTTTGGCT
TATGCTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAATCTTCCATTCCTC
CCAGGTACCGCTCAAAAGAAGAGAGAAGGTACTCTCAGAGGTGGTTACATTGTCGTTAGT
GAAACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGTTCAACATTGTGTT
GAAGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCCATGCACTGAACTT
TTCGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGGTTGTAGAAATCGT
ATCGCTATGGAAGCTGGTGTCACTTCATTCTGGTATAAATATGTTGGTCTTGATGGTAAA
GTCATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGTTATGAAACAATTA
GGTATGACTTCTNAAAATTTAGTAAACATTTCAAAACAATAAAAATAAAATAAAATAAAT
CAAAATCAAAATCTAAATTAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
NETRGLSMDAVAKAAXGHLGLPLGSAEIGAALFGNSLIYNPKXTRXLNRXYFVLXAGHGS
MFLYSWLHLSGYDVSIEXXKNFXQLNSXTPGHPKFHDXPGVEATTGPLGQGIANAVGIAS
PCKMAXGKFNTEQHQXFNQKVVVLV---

---FSDYLRPAIRLAALSHLPVVYIFTHDSVGVGEDGPTHQPVETVSGLRMIPNLDVIRP
ADPEETAAAFSLAYARADGPTLLSLTRQNLPFLPGTAQKKREGTLRGGYIVVSETAPLRM
ILIATGSEVQHCVEAAKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGCRNRIAMEAGV
TSFWYKYVGLDGKVIGIDRFGMSAPGNAVMKQLGMTSXNLVNISKQ*k*nkinqnqnln*
Translated Amino Acid sequence (All Frames) 
Frame A:
qmklev*awmqlpklpxvi*vyhlvllklxlhylvih*sitqxiqxg*ixiilx*xqvmv
qcfyivgyiyqdmmfqlxxlkisxn*iqxxqviqnfmthqalklplvh*vkvlqmplvlh
hhvkwxqvnsilnnikxlikr**f*l---

---ilrlsssshpfgcsltftsslhfhs*lcwcw*rwsnssts*nrfrssydsklgchss
srsrrnsccflfglcscrwsnpfiinsskssippryrskeerrysqrwlhcr**nrsikn
dlnchwfrsstlc*sr*iir**yscrlyamh*tfrssik*iqtirtskwl*ksyrygswc
hfilv*icws*w*shwy**iwyvctr*cryetirydfxkfskhfktikik*nkskskskl
n

Frame B:
k*n*rfkhgcscqsclwsfrfttwfc*nwxciiw*fidl*pkxykxvk*xlfcxkxrswf
nvfi*lvtfiri*cfn*xx*kfxtikfkxxrsskis*xtrr*syhwsirsryckcrwyci
tm*ngxr*iqy*ttsnx*skgssfsw---

---FSDYLRPAIRLAALSHLPVVYIFTHDSVGVGEDGPTHQPVETVSGLRMIPNLDVIRP
ADPEETAAAFSLAYARADGPTLLSLTRQNLPFLPGTAQKKREGTLRGGYIVVSETAPLRM
ILIATGSEVQHCVEAAKLLGDDIRVVSMPCTELFDRQSNEYKQSVLPSGCRNRIAMEAGV
TSFWYKYVGLDGKVIGIDRFGMSAPGNAVMKQLGMTSXNLVNISKQ*k*nkinqnqnln*

Frame C:
NETRGLSMDAVAKAAXGHLGLPLGSAEIGAALFGNSLIYNPKXTRXLNRXYFVLXAGHGS
MFLYSWLHLSGYDVSIEXXKNFXQLNSXTPGHPKFHDXPGVEATTGPLGQGIANAVGIAS
PCKMAXGKFNTEQHQXFNQKVVVLV---

---sqtifvqpsvwllshiyq*ftfslmtllvlvkmvqlinqlkpfqvfv*fqtwmsfvq
piqkkqlllslwlmlvpmvqpfyh*lvkifhssqvplkrrekvlsevvtlslvkplh*e*
s*lplvqkfnivlkplny*vmifvsslchalnfsivnqmntnnpyfqvvveivslwklvs
lhsginmlvlmvkslvlidlvclhqvmpl*nn*v*llki**tfqnnknkik*ikiki*ik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP624 (VHP624Q) /CSM/VH/VHP6-A/VHP624Q.Seq.d/                       2012   0.0
VHQ119 (VHQ119Q) /CSM/VH/VHQ1-A/VHQ119Q.Seq.d/                       1271   0.0
VHP168 (VHP168Q) /CSM/VH/VHP1-C/VHP168Q.Seq.d/                       1271   0.0
VHM858 (VHM858Q) /CSM/VH/VHM8-C/VHM858Q.Seq.d/                       1271   0.0
VHL536 (VHL536Q) /CSM/VH/VHL5-B/VHL536Q.Seq.d/                       1271   0.0
VHI236 (VHI236Q) /CSM/VH/VHI2-B/VHI236Q.Seq.d/                       1271   0.0
VHH745 (VHH745Q) /CSM/VH/VHH7-B/VHH745Q.Seq.d/                       1271   0.0
VHE496 (VHE496Q) /CSM/VH/VHE4-D/VHE496Q.Seq.d/                       1271   0.0
VFF246 (VFF246Q) /CSM/VF/VFF2-B/VFF246Q.Seq.d/                       1271   0.0
VFD821 (VFD821Q) /CSM/VF/VFD8-A/VFD821Q.Seq.d/                       1271   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...  1275   0.0    1
(BJ446544) Dictyostelium discoideum cDNA clone:ddv63e05, 3' ...  1275   0.0    1
(BJ446272) Dictyostelium discoideum cDNA clone:ddv62o06, 3' ...  1275   0.0    1
(BJ446078) Dictyostelium discoideum cDNA clone:ddv61g17, 3' ...  1275   0.0    1
(BJ444487) Dictyostelium discoideum cDNA clone:ddv56d16, 3' ...  1275   0.0    1
(BJ441631) Dictyostelium discoideum cDNA clone:ddv47g10, 3' ...  1275   0.0    1
(BJ441330) Dictyostelium discoideum cDNA clone:ddv46j11, 3' ...  1275   0.0    1
(BJ439285) Dictyostelium discoideum cDNA clone:ddv39p24, 3' ...  1275   0.0    1
(BJ430650) Dictyostelium discoideum cDNA clone:ddv8j06, 3' e...  1275   0.0    1
(BJ428287) Dictyostelium discoideum cDNA clone:ddv11k12, 3' ...  1275   0.0    1
dna update 2008.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556J0) RecName: Full=Transketolase;          Short=TK;        ...   433   e-120
(A6YB01) RecName: Full=Transketolase;          Short=TK;        ...   293   1e-77
AM746676_3991(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...   292   2e-77
CP001032_3788(CP001032|pid:none) Opitutus terrae PB90-1, complet...   291   4e-77
EF216683_1(EF216683|pid:none) Karlodinium micrum transketolase m...   282   2e-74
AJ821796_1(AJ821796|pid:none) Guillardia theta partial mRNA for ...   278   3e-73
EF216685_1(EF216685|pid:none) Bigelowiella natans transketolase ...   276   1e-72
EF216682_1(EF216682|pid:none) Karlodinium micrum transketolase m...   276   1e-72
EF216681_1(EF216681|pid:none) Isochrysis galbana putative plasti...   240   6e-62
AP009484_999(AP009484|pid:none) Macrococcus caseolyticus JCSC540...   239   1e-61
protein update 2009. 7.21
PSORT 

 psg: 0.75  gvh: 0.54  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHP624 is cyt
5' end seq. ID VHP624F
5' end seq. 
>VHP624F.Seq
CAAATGAAACTAGAGGTTTAAGCATGGATGCAGTTGCCAAAGCTGCCTNTGGTCATTTAG
GTTTACCACTTGGTTCTGCTGAAATTGGNGCTGCATTATTTGGTAATTCATTGATCTATA
ACCCAAAANATACAAGANGGTTAAATAGANATTATTTTGTNTTAAGNGCAGGTCATGGTT
CAATGTTTTTATATAGTTGGTTACATTTATCAGGATATGATGTTTCAATTGAANATNTTA
AAAATTTCANACAATTAAATTCAAANACNCCAGGTCATCCAAAATTTCATGACNCACCAG
GCGTTGAAGCTACCACTGGTCCATTAGGTCAAGGTATTGCAAATGCCGTTGGTATTGCAT
CACCATGTAAAATGGCANCAGGTAAATTCAATACTGAACAACATCAAATNTTTAATCAAA
AGGTAGTAGTTTTAGTTGGNNNNNNNNNN
Length of 5' end seq. 449
3' end seq. ID VHP624Z
3' end seq. 
>VHP624Z.Seq
NNNNNNNNNNATTCTCAGACTATCTTCGTCCAGCCATCCGTTTGGCTGCTCTCTCACATT
TACCAGTAGTTTACATTTTCACTCATGACTCTGTTGGTGTTGGTGAAGATGGTCCAACTC
ATCAACCAGTTGAAACCGTTTCAGGTCTTCGTATGATTCCAAACTTGGATGTCATTCGTC
CAGCCGATCCAGAAGAAACAGCTGCTGCTTTCTCTTTGGCTTATGCTCGTGCCGATGGTC
CAACCCTTTTATCATTAACTCGTCAAAATCTTCCATTCCTCCCAGGTACCGCTCAAAAGA
AGAGAGAAGGTACTCTCAGAGGTGGTTACATTGTCGTTAGTGAAACCGCTCCATTAAGAA
TGATCTTAATTGCCACTGGTTCAGAAGTTCAACATTGTGTTGAAGCCGCTAAATTATTAG
GTGATGATATTCGTGTCGTCTCTATGCCATGCACTGAACTTTTCGATCGTCAATCAAATG
AATACAAACAATCCGTACTTCCAAGTGGTTGTAGAAATCGTATCGCTATGGAAGCTGGTG
TCACTTCATTCTGGTATAAATATGTTGGTCTTGATGGTAAAGTCATTGGTATTGATAGAT
TTGGTATGTCTGCACCAGGTAATGCCGTTATGAAACAATTAGGTATGACTTCTNAAAATT
TAGTAAACATTTCAAAACAATAAAAATAAAATAAAATAAATCAAAATCAAAATCTAAATT
AAAT
Length of 3' end seq. 724
Connected seq. ID VHP624P
Connected seq. 
>VHP624P.Seq
CAAATGAAACTAGAGGTTTAAGCATGGATGCAGTTGCCAAAGCTGCCTNTGGTCATTTAG
GTTTACCACTTGGTTCTGCTGAAATTGGNGCTGCATTATTTGGTAATTCATTGATCTATA
ACCCAAAANATACAAGANGGTTAAATAGANATTATTTTGTNTTAAGNGCAGGTCATGGTT
CAATGTTTTTATATAGTTGGTTACATTTATCAGGATATGATGTTTCAATTGAANATNTTA
AAAATTTCANACAATTAAATTCAAANACNCCAGGTCATCCAAAATTTCATGACNCACCAG
GCGTTGAAGCTACCACTGGTCCATTAGGTCAAGGTATTGCAAATGCCGTTGGTATTGCAT
CACCATGTAAAATGGCANCAGGTAAATTCAATACTGAACAACATCAAATNTTTAATCAAA
AGGTAGTAGTTTTAGTTGG----------ATTCTCAGACTATCTTCGTCCAGCCATCCGT
TTGGCTGCTCTCTCACATTTACCAGTAGTTTACATTTTCACTCATGACTCTGTTGGTGTT
GGTGAAGATGGTCCAACTCATCAACCAGTTGAAACCGTTTCAGGTCTTCGTATGATTCCA
AACTTGGATGTCATTCGTCCAGCCGATCCAGAAGAAACAGCTGCTGCTTTCTCTTTGGCT
TATGCTCGTGCCGATGGTCCAACCCTTTTATCATTAACTCGTCAAAATCTTCCATTCCTC
CCAGGTACCGCTCAAAAGAAGAGAGAAGGTACTCTCAGAGGTGGTTACATTGTCGTTAGT
GAAACCGCTCCATTAAGAATGATCTTAATTGCCACTGGTTCAGAAGTTCAACATTGTGTT
GAAGCCGCTAAATTATTAGGTGATGATATTCGTGTCGTCTCTATGCCATGCACTGAACTT
TTCGATCGTCAATCAAATGAATACAAACAATCCGTACTTCCAAGTGGTTGTAGAAATCGT
ATCGCTATGGAAGCTGGTGTCACTTCATTCTGGTATAAATATGTTGGTCTTGATGGTAAA
GTCATTGGTATTGATAGATTTGGTATGTCTGCACCAGGTAATGCCGTTATGAAACAATTA
GGTATGACTTCTNAAAATTTAGTAAACATTTCAAAACAATAAAAATAAAATAAAATAAAT
CAAAATCAAAATCTAAATTAAAT
Length of connected seq. 1153
Full length Seq ID -
Full length Seq. -
Length of full length seq. -