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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP612
Library VH
(Link to library)
Clone ID VHP612
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12403-1|Contig-U12438-1
Original site URL
Representative seq. ID VHP612P
(Link to Original site)
Representative DNA sequence 
>VHP612 (VHP612Q) /CSM/VH/VHP6-A/VHP612Q.Seq.d/
ATTTTATTTTTATCTTAAAAAAAAAAACTATTGGGTTATCATTATTCAATTGCATTCTTT
CTTTTTTANACNCCGGAATAAAGATTTAATAAAACAATTTGTNCTAAAAAGAAAAAAAAA
AAAAATTAACCGGNTTTTTTNTTTTCNNTNTNATTTNATTTTNATTTTTNCAANCAAAAA
AAAAAXXXXXXXXXXCAATCAGAACAAGATGTTTATAATGTTTTCGCTTCCTATTGTTCA
GGTAATATCAAGAGACTACCATGGAATGATTCACCATTGTCATTGGAATCTGAAAATATT
AAAGAGCAATTAGTATTTTTAAATAAGAATGGTTTCCTTACAATCAATTCACAACCTGCA
ATCAATGGTTTACCATCAACCGATAAACAACATGGTTGGGGTGGTAAGAATGGTATTGTN
TACAAAAAAGCATACATTGAATTNTTTGCCTCACCAGAGAATACCTTAAAACTCATCGAA
TACTTTAAGAAATGTCCAATGATCACTTATCATGCAGTCAATCGTAAAGGTGATAGTAAA
ACCAATACCAAAGGAACTAACGCCGTCACTTGGGGTATCTTCCCAGGTAAGGAAATCATT
CAACCAACAGGTGNTGATCANAATTNTTTTATGGTTTGGAAGGATGAAGCTTTTGCTCTN
TGGGAAANTCNATGG
sequence update 2002.10.25
Translated Amino Acid sequence 
FYFYLKKKNYWVIIIQLHSFFFXXRNKDLIKQFVLKRKKKKINRXFXFXXXXIXIFXXKK
K---

---QSEQDVYNVFASYCSGNIKRLPWNDSPLSLESENIKEQLVFLNKNGFLTINSQPAIN
GLPSTDKQHGWGGKNGIVYKKAYIEXFASPENTLKLIEYFKKCPMITYHAVNRKGDSKTN
TKGTNAVTWGIFPGKEIIQPTGXDXNXFMVWKDEAFALWEXXW
Translated Amino Acid sequence (All Frames) 
Frame A:
ilfls*kkkllgyhysiafflfxtpe*rfnkticxkkkkkkn*pxfxfsxxfxfxfxxqk
k---

---QSEQDVYNVFASYCSGNIKRLPWNDSPLSLESENIKEQLVFLNKNGFLTINSQPAIN
GLPSTDKQHGWGGKNGIVYKKAYIEXFASPENTLKLIEYFKKCPMITYHAVNRKGDSKTN
TKGTNAVTWGIFPGKEIIQPTGXDXNXFMVWKDEAFALWEXXW

Frame B:
FYFYLKKKNYWVIIIQLHSFFFXXRNKDLIKQFVLKRKKKKINRXFXFXXXXIXIFXXKK
K---

---nqnkmfimfslpivqvisrdyhgmihhchwnlkilksn*yf*irmvslqsihnlqsm
vyhqpinnmvgvvrmvlxtkkhtlnxlphqrip*nssntlrnvq*slimqsivkvivkpi
pkeltpslgvssqvrksfnqqvxixixlwfgrmklllxgkxx

Frame C:
fifilkkktiglslfncilsflxxgiki**nnlx*kekkkkltgffxfxxixfxfxqxkk
k---

---irtrcl*cfrfllfr*yqettme*ftivigi*ky*raisifk*ewfpynqfttcnqw
ftinr*ttwlgw*ewycxqksih*ixcltreylkthril*emsndhlscsqs*r***nqy
qrn*rrhlgylpr*gnhstnrx*sxxfygleg*sfcsxgxsm
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP612 (VHP612Q) /CSM/VH/VHP6-A/VHP612Q.Seq.d/                        904   0.0
VHB582 (VHB582Q) /CSM/VH/VHB5-D/VHB582Q.Seq.d/                        892   0.0
VSG248 (VSG248Q) /CSM/VS/VSG2-B/VSG248Q.Seq.d/                        821   0.0
CHI821 (CHI821Q) /CSM/CH/CHI8-A/CHI821Q.Seq.d/                        630   e-180
VHI819 (VHI819Q) /CSM/VH/VHI8-A/VHI819Q.Seq.d/                        444   e-124
VHE341 (VHE341Q) /CSM/VH/VHE3-B/VHE341Q.Seq.d/                        137   2e-31
VHC790 (VHC790Q) /CSM/VH/VHC7-D/VHC790Q.Seq.d/                        131   2e-29
VSE889 (VSE889Q) /CSM/VS/VSE8-D/VSE889Q.Seq.d/                         36   0.67
VHE191 (VHE191Q) /CSM/VH/VHE1-D/VHE191Q.Seq.d/                         36   0.67
VFF786 (VFF786Q) /CSM/VF/VFF7-D/VFF786Q.Seq.d/                         36   0.67
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446233) Dictyostelium discoideum cDNA clone:ddv62g04, 3' ...   904   0.0    1
(BJ437600) Dictyostelium discoideum cDNA clone:ddv34c21, 3' ...   892   0.0    1
(AU266191) Dictyostelium discoideum vegetative cDNA clone:VS...   722   0.0    1
(BJ380733) Dictyostelium discoideum cDNA clone:ddc39j06, 3' ...   630   e-176  1
(BJ441850) Dictyostelium discoideum cDNA clone:ddv48f06, 3' ...   444   e-120  1
(AU266192) Dictyostelium discoideum vegetative cDNA clone:VS...   381   e-116  2
(BJ427331) Dictyostelium discoideum cDNA clone:ddv62g04, 5' ...   137   1e-31  2
(BJ419657) Dictyostelium discoideum cDNA clone:ddv36d23, 5' ...   131   7e-30  2
(BJ423101) Dictyostelium discoideum cDNA clone:ddv48f06, 5' ...   137   6e-28  1
(BJ420424) Dictyostelium discoideum cDNA clone:ddv39b11, 5' ...   137   6e-28  1
dna update 2008.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q60HE5) RecName: Full=Methylenetetrahydrofolate reductase;     ...   167   3e-40
(P42898) RecName: Full=Methylenetetrahydrofolate reductase;     ...   166   5e-40
AK312907_1(AK312907|pid:none) Homo sapiens cDNA, FLJ93354, highl...   166   5e-40
AM920430_48(AM920430|pid:none) Penicillium chrysogenum Wisconsin...   166   8e-40
A47328_1(A47328|pid:none) Sequence 3 from Patent WO9533054.  &AF...   165   1e-39
(Q75HE6) RecName: Full=Probable methylenetetrahydrofolate reduct...   165   1e-39
BC053509_1(BC053509|pid:none) Homo sapiens 5,10-methylenetetrahy...   165   1e-39
DQ862830_1(DQ862830|pid:none) Triticum monococcum 5,10-methylene...   164   2e-39
(Q5I598) RecName: Full=Methylenetetrahydrofolate reductase;     ...   163   5e-39
(Q9SE94) RecName: Full=Methylenetetrahydrofolate reductase 1;   ...   162   1e-38
protein update 2009. 7.21
PSORT 

 psg: 0.88  gvh: 0.51  alm: 0.49  top: 0.50  tms: 0.00  mit: 0.33  mip: 0.08
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHP612 is nuc
5' end seq. ID VHP612F
5' end seq. 
>VHP612F.Seq
ATTTTATTTTTATCTTAAAAAAAAAAACTATTGGGTTATCATTATTCAATTGCATTCTTT
CTTTTTTANACNCCGGAATAAAGATTTAATAAAACAATTTGTNCTAAAAAGAAAAAAAAA
AAAAATTAACCGGNTTTTTTNTTTTCNNTNTNATTTNATTTTNATTTTTNCAANCAAAAA
AAAAANNNNNNNNNN
Length of 5' end seq. 195
3' end seq. ID VHP612Z
3' end seq. 
>VHP612Z.Seq
NNNNNNNNNNCAATCAGAACAAGATGTTTATAATGTTTTCGCTTCCTATTGTTCAGGTAA
TATCAAGAGACTACCATGGAATGATTCACCATTGTCATTGGAATCTGAAAATATTAAAGA
GCAATTAGTATTTTTAAATAAGAATGGTTTCCTTACAATCAATTCACAACCTGCAATCAA
TGGTTTACCATCAACCGATAAACAACATGGTTGGGGTGGTAAGAATGGTATTGTNTACAA
AAAAGCATACATTGAATTNTTTGCCTCACCAGAGAATACCTTAAAACTCATCGAATACTT
TAAGAAATGTCCAATGATCACTTATCATGCAGTCAATCGTAAAGGTGATAGTAAAACCAA
TACCAAAGGAACTAACGCCGTCACTTGGGGTATCTTCCCAGGTAAGGAAATCATTCAACC
AACAGGTGNTGATCANAATTNTTTTATGGTTTGGAAGGATGAAGCTTTTGCTCTNTGGGA
AANTCNATGG
Length of 3' end seq. 490
Connected seq. ID VHP612P
Connected seq. 
>VHP612P.Seq
ATTTTATTTTTATCTTAAAAAAAAAAACTATTGGGTTATCATTATTCAATTGCATTCTTT
CTTTTTTANACNCCGGAATAAAGATTTAATAAAACAATTTGTNCTAAAAAGAAAAAAAAA
AAAAATTAACCGGNTTTTTTNTTTTCNNTNTNATTTNATTTTNATTTTTNCAANCAAAAA
AAAAA----------CAATCAGAACAAGATGTTTATAATGTTTTCGCTTCCTATTGTTCA
GGTAATATCAAGAGACTACCATGGAATGATTCACCATTGTCATTGGAATCTGAAAATATT
AAAGAGCAATTAGTATTTTTAAATAAGAATGGTTTCCTTACAATCAATTCACAACCTGCA
ATCAATGGTTTACCATCAACCGATAAACAACATGGTTGGGGTGGTAAGAATGGTATTGTN
TACAAAAAAGCATACATTGAATTNTTTGCCTCACCAGAGAATACCTTAAAACTCATCGAA
TACTTTAAGAAATGTCCAATGATCACTTATCATGCAGTCAATCGTAAAGGTGATAGTAAA
ACCAATACCAAAGGAACTAACGCCGTCACTTGGGGTATCTTCCCAGGTAAGGAAATCATT
CAACCAACAGGTGNTGATCANAATTNTTTTATGGTTTGGAAGGATGAAGCTTTTGCTCTN
TGGGAAANTCNATGG
Length of connected seq. 665
Full length Seq ID -
Full length Seq. -
Length of full length seq. -