>AFO844 (AFO844Q) /CSM/AF/AFO8-B/AFO844Q.Seq.d/
AATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATAXXXXXXXXXXAAACCAGTCGATTGGGTTGACGTAAAGGAAA
TCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTG
ATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCC
CAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAG
AATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATG
AACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTG
GTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTNTG
AAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCG
CCAAAGCTGAANAGANAAAGAAAGCTGGCGAAAAATTAGCTGCCGAAAAAGCTGCTGAAA
AAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAA
CTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT

nhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVD---

---KPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPE
YKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNM
IVXDSVEEAKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKAGEKLAAEKAAEKEAEEAD
EEEEEVAEEDLVKTDXKKEEVKKSTKK

Frame A:
nhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWH
KEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQ
KVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEIN
KVETDQLTHQYTLVISPDNTYKVLVD---

---KPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPE
YKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNM
IVXDSVEEAKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKAGEKLAAEKAAEKEAEEAD
EEEEEVAEEDLVKTDXKKEEVKKSTKK

Frame B:
iiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigi
kkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnk
klivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkst
klkpin*lintlwsfhqitptkyssi---

---nqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqn
tkvngkqrksqiqntkvngyth*litqntlktmnstslmisvlsvsnygklnlvpslti*
ssptlskklkisxkrpllptkkpkrkclttskppklxrxrklakn*lpkkllkkkpkklm
kkkkkslkki*lklmxkkkksknqpkk

Frame C:
ssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*
rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tk
s*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqq
s*nrsinssihfghftr*hlqstrr---

---tsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsri
qr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qyd
rxrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*xxkeswrkiscrksc*krsrrs**
rrrrsr*rrfs*n*xqkrrsqkinqks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                       1019   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                       1019   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                       1019   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1019   0.0
VFH121 (VFH121Q) /CSM/VF/VFH1-A/VFH121Q.Seq.d/                       1019   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                       1019   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1019   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1019   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1019   0.0
VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1019   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1011  0.0  4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  1e-23  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  9e-22  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  8e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  8e-11  2
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  5e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  5e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   354   e-173
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   224   e-100
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   218   e-100
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   223   e-100
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   221   1e-99
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   221   2e-99
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   221   3e-99
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   216   4e-99
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   214   1e-98
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   221   1e-98

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFO844 is exc

>AFO844F.Seq
AATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATA----------

>AFO844Z.Seq
----------AAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGT
TAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACC
AGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGA
ATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGT
ACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGA
TCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATAT
GATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAA
CCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAANAGANAAA
GAAAGCTGGCGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGA
TGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGA
AGTCAAAAAATCAACCAAAAAAGT

>AFO844P.Seq
AATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATG
AGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTT
CATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCAT
AAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAA
AATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTC
CCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAA
AAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCA
TTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAG
AGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAAC
AAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACC
TACAAAGTACTCGTCGATA----------AAACCAGTCGATTGGGTTGACGTAAAGGAAA
TCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTG
ATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCC
CAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAG
AATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATG
AACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTG
GTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTNTG
AAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCG
CCAAAGCTGAANAGANAAAGAAAGCTGGCGAAAAATTAGCTGCCGAAAAAGCTGCTGAAA
AAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAA
CTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT