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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP594
Library VH
(Link to library)
Clone ID VHP594
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHP594P
(Link to Original site)
Representative DNA sequence 
>VHP594 (VHP594Q) /CSM/VH/VHP5-D/VHP594Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGXXXXXXXXXXTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGA
TAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAA
CGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGA
TTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACAT
GAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGG
TGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTC
CCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGG
TGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTAT
CGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCAC
CGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAG
AAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATTTAAGTCGCACTTNNAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxvfk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxvfk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*---

---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqf
kshxxfs

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srtxsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP769 (VHP769Q) /CSM/VH/VHP7-C/VHP769Q.Seq.d/                       2567   0.0
VHP594 (VHP594Q) /CSM/VH/VHP5-D/VHP594Q.Seq.d/                       2567   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1445   0.0
VHP478 (VHP478Q) /CSM/VH/VHP4-D/VHP478Q.Seq.d/                       1445   0.0
VHP408 (VHP408Q) /CSM/VH/VHP4-A/VHP408Q.Seq.d/                       1445   0.0
VHP402 (VHP402Q) /CSM/VH/VHP4-A/VHP402Q.Seq.d/                       1445   0.0
VHO715 (VHO715Q) /CSM/VH/VHO7-A/VHO715Q.Seq.d/                       1445   0.0
VHK821 (VHK821Q) /CSM/VH/VHK8-A/VHK821Q.Seq.d/                       1445   0.0
VHK654 (VHK654Q) /CSM/VH/VHK6-C/VHK654Q.Seq.d/                       1445   0.0
VHK634 (VHK634Q) /CSM/VH/VHK6-B/VHK634Q.Seq.d/                       1445   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446651) Dictyostelium discoideum cDNA clone:ddv63l12, 3' ...  1445   0.0    1
(BJ446490) Dictyostelium discoideum cDNA clone:ddv62k23, 3' ...  1445   0.0    1
(BJ446408) Dictyostelium discoideum cDNA clone:ddv62j17, 3' ...  1445   0.0    1
(BJ446177) Dictyostelium discoideum cDNA clone:ddv61l20, 3' ...  1445   0.0    1
(BJ445965) Dictyostelium discoideum cDNA clone:ddv61p02, 3' ...  1445   0.0    1
(BJ445910) Dictyostelium discoideum cDNA clone:ddv61d02, 3' ...  1445   0.0    1
(BJ445650) Dictyostelium discoideum cDNA clone:ddv60n03, 3' ...  1445   0.0    1
(BJ443343) Dictyostelium discoideum cDNA clone:ddv52k14, 3' ...  1445   0.0    1
(BJ443220) Dictyostelium discoideum cDNA clone:ddv52c10, 3' ...  1445   0.0    1
(BJ443175) Dictyostelium discoideum cDNA clone:ddv52j06, 3' ...  1445   0.0    1
dna update 2008.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   9e-81
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   3e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   3e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   297   7e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   9e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   9e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   9e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   296   9e-79
protein update 2009. 7.21
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHP594 is cyt
5' end seq. ID VHP594F
5' end seq. 
>VHP594F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGNNNNNNNNNN
Length of 5' end seq. 616
3' end seq. ID VHP594Z
3' end seq. 
>VHP594Z.Seq
NNNNNNNNNNTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCA
CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGC
CAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTT
CGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAAC
TCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTAT
TTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTC
CCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGA
TTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTC
AATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAG
AAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAAT
TTAAGTCGCACTTNNAGTTTTCAAA
Length of 3' end seq. 745
Connected seq. ID VHP594P
Connected seq. 
>VHP594P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAG----------TCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGA
TAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAA
CGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGA
TTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACAT
GAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGG
TGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTC
CCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGG
TGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTAT
CGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCAC
CGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAG
AAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATTTAAGTCGCACTTNNAGTTTTCAAA
Length of connected seq. 1341
Full length Seq ID -
Full length Seq. -
Length of full length seq. -