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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP589
Library VH
(Link to library)
Clone ID VHP589
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHP589P
(Link to Original site)
Representative DNA sequence 
>VHP589 (VHP589Q) /CSM/VH/VHP5-D/VHP589Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAXXXXXXXXXXCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAG
GTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCA
AAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCA
CCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTG
TACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAG
AAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAG
CTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAA
ATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqlx*nqxt**kllpqktllvvftlysivvxvll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqfkshfefskivikskknfkkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---rr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpqrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkrywwyllctqssxrycy
w*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkekrfsprnprs*qiprktlnnlsrtlsfqk**snlkkilkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP589 (VHP589Q) /CSM/VH/VHP5-D/VHP589Q.Seq.d/                       2405   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1281   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1281   0.0
VHP213 (VHP213Q) /CSM/VH/VHP2-A/VHP213Q.Seq.d/                       1281   0.0
VHK684 (VHK684Q) /CSM/VH/VHK6-D/VHK684Q.Seq.d/                       1281   0.0
VHK214 (VHK214Q) /CSM/VH/VHK2-A/VHK214Q.Seq.d/                       1281   0.0
VHJ652 (VHJ652Q) /CSM/VH/VHJ6-C/VHJ652Q.Seq.d/                       1281   0.0
VHI289 (VHI289Q) /CSM/VH/VHI2-D/VHI289Q.Seq.d/                       1281   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1281   0.0
VHH827 (VHH827Q) /CSM/VH/VHH8-B/VHH827Q.Seq.d/                       1281   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446440) Dictyostelium discoideum cDNA clone:ddv62a23, 3' ...  1281   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1281   0.0    1
(BJ445933) Dictyostelium discoideum cDNA clone:ddv61i04, 3' ...  1281   0.0    1
(BJ443409) Dictyostelium discoideum cDNA clone:ddv52g22, 3' ...  1281   0.0    1
(BJ442845) Dictyostelium discoideum cDNA clone:ddv51k04, 3' ...  1281   0.0    1
(BJ442655) Dictyostelium discoideum cDNA clone:ddv50g14, 3' ...  1281   0.0    1
(BJ441752) Dictyostelium discoideum cDNA clone:ddv47a24, 3' ...  1281   0.0    1
(BJ441320) Dictyostelium discoideum cDNA clone:ddv46h12, 3' ...  1281   0.0    1
(BJ441306) Dictyostelium discoideum cDNA clone:ddv46f08, 3' ...  1281   0.0    1
(BJ438985) Dictyostelium discoideum cDNA clone:ddv39g05, 3' ...  1281   0.0    1
dna update 2008.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   263   8e-69
AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   8e-69
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   262   2e-68
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   261   5e-68
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   259   1e-67
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   259   1e-67
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   259   1e-67
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   259   1e-67
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   258   3e-67
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   3e-67
protein update 2009. 7.21
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHP589 is cyt
5' end seq. ID VHP589F
5' end seq. 
>VHP589F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHP589Z
3' end seq. 
>VHP589Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTA
TGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAA
TCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of 3' end seq. 690
Connected seq. ID VHP589P
Connected seq. 
>VHP589P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGA----------CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAG
GTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCA
AAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCA
CCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTG
TACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAG
AAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAG
CTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAA
ATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of connected seq. 1287
Full length Seq ID -
Full length Seq. -
Length of full length seq. -