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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO841
Library AF
(Link to library)
Clone ID AFO841
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFO841P
(Link to Original site)
Representative DNA sequence 
>AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/
TTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATC
AATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTA
CACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAAC
TGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGG
TGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATC
AGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAA
AGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGC
TGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAG
ATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGC
TATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTAT
GGGTTCAGAAGXXXXXXXXXXTAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTA
TCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTG
AACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGG
ATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAA
ACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCA
TTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCAT
ACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCA
ACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAG
TTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCAT
CATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATT
TAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGAT
TAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGNAAAACCATAACTATGC
NGGTTAACTTTCCGTAATTTNATTTACTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
YFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAST
GIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNK
GKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLA
MQEFMILPVGAKDFNEAYRMGSE---

---KNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAAS
EFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLT
ASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVM
VSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKTITMXVNFP
*fxll
Translated Amino Acid sequence (All Frames) 
Frame A:
lfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircin
wyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*
r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**is
yariydftswc*rfq*slsygfr---

---*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlp
lnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*
qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvs
wlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*xkp*lcxltf
rnxiy

Frame B:
YFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAST
GIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNK
GKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLA
MQEFMILPVGAKDFNEAYRMGSE---

---kechqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl
*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*in
sis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgch
g*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvrxnhnyag*ls
vixft

Frame C:
iffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhql
vfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqik
vn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*l
cknl*fyqlvlkismkpivwvqk---

---KNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAAS
EFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLT
ASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVM
VSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKTITMXVNFP
*fxll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/                       2573   0.0
AFJ620 (AFJ620Q) /CSM/AF/AFJ6-A/AFJ620Q.Seq.d/                       1392   0.0
SFG643 (SFG643Q) /CSM/SF/SFG6-B/SFG643Q.Seq.d/                       1388   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1382   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1380   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1380   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1380   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1378   0.0
AFC510 (AFC510Q) /CSM/AF/AFC5-A/AFC510Q.Seq.d/                       1378   0.0
AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       1376   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X80474|X80474.1 N.frontalis enolase gene.                         64  4e-77  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  8e-73  11
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-63  10
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-63  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76  4e-63  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76  3e-61  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92  4e-54  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  1e-48  5
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  2e-47  10
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50  5e-47  10
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   452   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   347   e-164
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   339   e-161
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   336   e-158
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   332   e-158
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   340   e-157
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   323   e-157
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   323   e-157
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   342   e-156
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   341   e-156
protein update 2009. 4.26
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFO841 is nuc
5' end seq. ID AFO841F
5' end seq. 
>AFO841F.Seq
TTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATC
AATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTA
CACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAAC
TGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGG
TGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATC
AGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAA
AGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGC
TGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAG
ATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGC
TATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTAT
GGGTTCAGAAG----------
Length of 5' end seq. 611
3' end seq. ID AFO841Z
3' end seq. 
>AFO841Z.Seq
----------TAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGT
GATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAA
CTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCC
TCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGT
TCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATAT
CCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTA
ACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGA
ATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATT
GGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTC
ATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGT
TTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATAC
AATCAACTTGTTAGAATTAACGAAGAGTTAGGNAAAACCATAACTATGCNGGTTAACTTT
CCGTAATTTNATTTACTC
Length of 3' end seq. 728
Connected seq. ID AFO841P
Connected seq. 
>AFO841P.Seq
TTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATC
AATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTA
CACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAAC
TGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGG
TGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATC
AGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAA
AGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGC
TGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAG
ATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGC
TATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTAT
GGGTTCAGAAG----------TAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTA
TCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTG
AACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGG
ATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAA
ACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCA
TTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCAT
ACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCA
ACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAG
TTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCAT
CATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATT
TAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGAT
TAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGNAAAACCATAACTATGC
NGGTTAACTTTCCGTAATTTNATTTACTC
Length of connected seq. 1339
Full length Seq ID -
Full length Seq. -
Length of full length seq. -