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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP546
Library VH
(Link to library)
Clone ID VHP546
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11694-1
Original site URL
Representative seq. ID VHP546P
(Link to Original site)
Representative DNA sequence 
>VHP546 (VHP546Q) /CSM/VH/VHP5-B/VHP546Q.Seq.d/
ATAAAAACAAATATTTTTATTTATTTATTTTAATCATTGATAACCTAATAATTATTTAAT
TTTTTTAATTTATTTAGAAAAAAAAAAATAATAAATTATTTGTTAAATAAATAATTTTAT
TATTTTAATAATATATAAAAAAATATATATATAAAGAAATGACAAATAGTAATTTTAATC
ATGGTAATCATGGTAGTAATTTACCAGCANGGTTATTTACAAAACAATCACAGGCATTAA
TTTATAATTATAAAGAAGCAGCAGNCCAAAGAANGTTANATTTTGATAATGTTTCACAAA
GGGATACNCCATCAGNGGGNGGGTTAATTCATCCAGGATCCGATGGNGGTATGTATAAAG
CATTCTTTGGATTTAAAGAGTTGGTAATACCAGTTTATAATTCAGTATCANAAGCATGTC
ANCAATGTCCAAATGCANATGTATTTTTAAATTTTGCATCACATCGTTCAGCTTACCAAT
CATCGTTATTGGCATTAANANAACCATCGATTCAAACCGTTGTTATCATTGCAGAGXXXX
XXXXXXCATACGCTTCCAAGTTCTTTGAAATGTGTTTGAAACTAGTTGCCGATCATGGTC
CATGTGTTAGTGGTGCTCATAATACAATCGTTGCTGCTCGTGCTGGTAAGGATTTAGTAT
CAAGTNTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCGATGATA
GTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTGTTGAAG
GTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCTGCCGATG
AAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTNTCATCAACAA
AATATTTAGAATACGCATTGGAAGTTGAAAAATATACCCTTCAAAAAGCAAATAATTTAA
TTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATCCGGTT
TATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTTCTTTA
TTGTTGGTANAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAAACAAG
GACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAAATATA
AATATAAAAATAAAAATAAAATAAAATAAAATAAAACCAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
knkyfylfiliidnliii*ff*fi*kkknnklfvk*iillf**YIKKYIYKEMTNSNFNH
GNHGSNLPAXLFTKQSQALIYNYKEAAXQRXLXFDNVSQRDTPSXGGLIHPGSDGGMYKA
FFGFKELVIPVYNSVSXACXQCPNAXVFLNFASHRSAYQSSLLALXXPSIQTVVIIAE--
-

---YASKFFEMCLKLVADHGPCVSGAHNTIVAARAGKDLVSSXVSGLLTIGPRFGGAIDD
SARVFQDAVDNNLQPSQFVEGMKSKGKRIPGIGHLIKSADEIDKRVVLLKDYAFTHXSST
KYLEYALEVEKYTLQKANNLILNVDGCIGVLFLDLLHSSGLFTQHEIKEIIDVGYLNGFF
IVGXSVGLIGHALDQRRNKQGLYRHQADDVHYAL*nfnkykyknknkik*nktkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
iktnifiylf*slit**lfnffnlfrkkkiinyllnk*fyyfnni*kniyikk*qivili
mvimvviyqxgylqnnhrh*fiiikkqqxkexxilimfhkgixhqxxg*fiqdpmxvcik
hsldlksw*yqfiiqyxkhvxnvqmxmyf*ilhhivqltnhrywh*xnhrfkpllslq--
-

---htlpsslkcv*n*lpimvhvlvvliiqslllvlvri*yqvlfqvy*qldhvlvvpsm
ivlvyskmqsitiynhhnllkv*nqrvkefqvlvi*lnlpmksike*yy*kimlslixhq
qni*nthwklknipfkkqii*f*mlmvvlvyyf*iyyihpvyslnmklkkl*mlvi*mvs
lllvxvlv*svth*inveinkdfidtklmmfimpfkilininikikik*nkikpkkk

Frame B:
*kqiflfiyfnh**pnnylifliylekkk**iic*innfiiliiykkiyi*rndk**f*s
w*sw**ftsxviyktitginl*l*rssxpkxvxf**cftkgyxixgxvnssrirwxyv*s
ilwi*rvgntsl*fsixsmsxmskcxcifkfcitsfslpiivigixxtidsnrcyhcr--
-

---irfqvl*nvfetscrswsmc*wcs*ynrccscw*gfsikxcfrfinnwttfwwchr*
*csciprcsr*qfttitic*ryeikg*knsrywsfn*icr*nr*kssiikrlcfhsfxin
kifririgs*kiypsksk*fnfkc*wlywciifrfitfirfihst*n*rnyrcwlfkwfl
ycwxkcwfnrsrirst*k*trtl*tps**cslcplkf**i*i*k*k*nkik*nqkk

Frame C:
knkyfylfiliidnliii*ff*fi*kkknnklfvk*iillf**YIKKYIYKEMTNSNFNH
GNHGSNLPAXLFTKQSQALIYNYKEAAXQRXLXFDNVSQRDTPSXGGLIHPGSDGGMYKA
FFGFKELVIPVYNSVSXACXQCPNAXVFLNFASHRSAYQSSLLALXXPSIQTVVIIAE--
-

---YASKFFEMCLKLVADHGPCVSGAHNTIVAARAGKDLVSSXVSGLLTIGPRFGGAIDD
SARVFQDAVDNNLQPSQFVEGMKSKGKRIPGIGHLIKSADEIDKRVVLLKDYAFTHXSST
KYLEYALEVEKYTLQKANNLILNVDGCIGVLFLDLLHSSGLFTQHEIKEIIDVGYLNGFF
IVGXSVGLIGHALDQRRNKQGLYRHQADDVHYAL*nfnkykyknknkik*nktkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP546 (VHP546Q) /CSM/VH/VHP5-B/VHP546Q.Seq.d/                       1844   0.0
VHI728 (VHI728Q) /CSM/VH/VHI7-B/VHI728Q.Seq.d/                       1241   0.0
VHB747 (VHB747Q) /CSM/VH/VHB7-B/VHB747Q.Seq.d/                       1241   0.0
VHA674 (VHA674Q) /CSM/VH/VHA6-D/VHA674Q.Seq.d/                       1241   0.0
VHA368 (VHA368Q) /CSM/VH/VHA3-C/VHA368Q.Seq.d/                       1241   0.0
CHL239 (CHL239Q) /CSM/CH/CHL2-B/CHL239Q.Seq.d/                       1241   0.0
CHE602 (CHE602Q) /CSM/CH/CHE6-A/CHE602Q.Seq.d/                       1241   0.0
VFK504 (VFK504Q) /CSM/VF/VFK5-A/VFK504Q.Seq.d/                       1233   0.0
VHN804 (VHN804Q) /CSM/VH/VHN8-A/VHN804Q.Seq.d/                       1227   0.0
VHC389 (VHC389Q) /CSM/VH/VHC3-D/VHC389Q.Seq.d/                       1201   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446332) Dictyostelium discoideum cDNA clone:ddv62k11, 3' ...  1241   0.0    1
(BJ441945) Dictyostelium discoideum cDNA clone:ddv48h07, 3' ...  1241   0.0    1
(BJ437494) Dictyostelium discoideum cDNA clone:ddv34n11, 3' ...  1241   0.0    1
(BJ436960) Dictyostelium discoideum cDNA clone:ddv32c20, 3' ...  1241   0.0    1
(BJ436579) Dictyostelium discoideum cDNA clone:ddv31h17, 3' ...  1241   0.0    1
(BJ382337) Dictyostelium discoideum cDNA clone:ddc44m10, 3' ...  1241   0.0    1
(BJ378451) Dictyostelium discoideum cDNA clone:ddc31c02, 3' ...  1241   0.0    1
(BJ433497) Dictyostelium discoideum cDNA clone:ddv22g01, 3' ...  1233   0.0    1
(BJ444988) Dictyostelium discoideum cDNA clone:ddv58h02, 3' ...  1201   0.0    2
(BJ437909) Dictyostelium discoideum cDNA clone:ddv35b23, 3' ...  1201   0.0    1
dna update 2008.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YA0) RecName: Full=Probable ATP-citrate synthase;          E...   416   e-114
AP002883_22(AP002883|pid:none) Oryza sativa Japonica Group genom...   298   3e-79
AC020580_2(AC020580|pid:none) Arabidopsis thaliana chromosome 3 ...   292   1e-77
AB023034_12(AB023034|pid:none) Arabidopsis thaliana genomic DNA,...   292   1e-77
AJ344107_1(AJ344107|pid:none) Lupinus albus mRNA for ATP citrate...   291   3e-77
EF571302_1(EF571302|pid:none) Glycyrrhiza uralensis ATP citrate ...   291   4e-77
AF290958_1(AF290958|pid:none) Capsicum annuum ATP:citrate lyase ...   281   3e-74
AJ243483_1(AJ243483|pid:none) Cyanophora paradoxa mRNA for ATP c...   273   9e-72
BC080908_1(BC080908|pid:none) Xenopus tropicalis acly protein, m...   255   2e-66
AJ851548_1(AJ851548|pid:none) Gallus gallus mRNA for hypothetica...   255   3e-66
protein update 2009. 7.21
PSORT 

 psg: 0.83  gvh: 0.50  alm: 0.29  top: 0.47  tms: 0.07  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHP546 is cyt
5' end seq. ID VHP546F
5' end seq. 
>VHP546F.Seq
ATAAAAACAAATATTTTTATTTATTTATTTTAATCATTGATAACCTAATAATTATTTAAT
TTTTTTAATTTATTTAGAAAAAAAAAAATAATAAATTATTTGTTAAATAAATAATTTTAT
TATTTTAATAATATATAAAAAAATATATATATAAAGAAATGACAAATAGTAATTTTAATC
ATGGTAATCATGGTAGTAATTTACCAGCANGGTTATTTACAAAACAATCACAGGCATTAA
TTTATAATTATAAAGAAGCAGCAGNCCAAAGAANGTTANATTTTGATAATGTTTCACAAA
GGGATACNCCATCAGNGGGNGGGTTAATTCATCCAGGATCCGATGGNGGTATGTATAAAG
CATTCTTTGGATTTAAAGAGTTGGTAATACCAGTTTATAATTCAGTATCANAAGCATGTC
ANCAATGTCCAAATGCANATGTATTTTTAAATTTTGCATCACATCGTTCAGCTTACCAAT
CATCGTTATTGGCATTAANANAACCATCGATTCAAACCGTTGTTATCATTGCAGAGNNNN
NNNNNN
Length of 5' end seq. 546
3' end seq. ID VHP546Z
3' end seq. 
>VHP546Z.Seq
NNNNNNNNNNCATACGCTTCCAAGTTCTTTGAAATGTGTTTGAAACTAGTTGCCGATCAT
GGTCCATGTGTTAGTGGTGCTCATAATACAATCGTTGCTGCTCGTGCTGGTAAGGATTTA
GTATCAAGTNTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCGAT
GATAGTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTGTT
GAAGGTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCTGCC
GATGAAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTNTCATCA
ACAAAATATTTAGAATACGCATTGGAAGTTGAAAAATATACCCTTCAAAAAGCAAATAAT
TTAATTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATCC
GGTTTATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTTC
TTTATTGTTGGTANAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAAA
CAAGGACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAAA
TATAAATATAAAAATAAAAATAAAATAAAATAAAATAAAACCAAAAAAAAAA
Length of 3' end seq. 712
Connected seq. ID VHP546P
Connected seq. 
>VHP546P.Seq
ATAAAAACAAATATTTTTATTTATTTATTTTAATCATTGATAACCTAATAATTATTTAAT
TTTTTTAATTTATTTAGAAAAAAAAAAATAATAAATTATTTGTTAAATAAATAATTTTAT
TATTTTAATAATATATAAAAAAATATATATATAAAGAAATGACAAATAGTAATTTTAATC
ATGGTAATCATGGTAGTAATTTACCAGCANGGTTATTTACAAAACAATCACAGGCATTAA
TTTATAATTATAAAGAAGCAGCAGNCCAAAGAANGTTANATTTTGATAATGTTTCACAAA
GGGATACNCCATCAGNGGGNGGGTTAATTCATCCAGGATCCGATGGNGGTATGTATAAAG
CATTCTTTGGATTTAAAGAGTTGGTAATACCAGTTTATAATTCAGTATCANAAGCATGTC
ANCAATGTCCAAATGCANATGTATTTTTAAATTTTGCATCACATCGTTCAGCTTACCAAT
CATCGTTATTGGCATTAANANAACCATCGATTCAAACCGTTGTTATCATTGCAGAG----
------CATACGCTTCCAAGTTCTTTGAAATGTGTTTGAAACTAGTTGCCGATCATGGTC
CATGTGTTAGTGGTGCTCATAATACAATCGTTGCTGCTCGTGCTGGTAAGGATTTAGTAT
CAAGTNTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCGATGATA
GTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTGTTGAAG
GTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCTGCCGATG
AAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTNTCATCAACAA
AATATTTAGAATACGCATTGGAAGTTGAAAAATATACCCTTCAAAAAGCAAATAATTTAA
TTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATCCGGTT
TATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTTCTTTA
TTGTTGGTANAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAAACAAG
GACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAAATATA
AATATAAAAATAAAAATAAAATAAAATAAAATAAAACCAAAAAAAAAA
Length of connected seq. 1238
Full length Seq ID -
Full length Seq. -
Length of full length seq. -