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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP536
Library VH
(Link to library)
Clone ID VHP536
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15690-1
Original site URL
Representative seq. ID VHP536P
(Link to Original site)
Representative DNA sequence 
>VHP536 (VHP536Q) /CSM/VH/VHP5-B/VHP536Q.Seq.d/
AATTAATTTTATTTTAATATAAAACTCATAATTTTATATATAAAATAAAAAAATAAAATA
AAAATGAACAAAGAAGAATATTCAGATATTTCAGACTCTGAATCAGAGGAAGTTCATGAA
ACAAACAATCATAATGAACACGAACACGAAGAAGAAGATGACACACCAGAAATTGTTGTA
CCAGAACGTAAATTCTTAAAAGAAGATGAAGATTATTCAGTTCCATTCCCAGTCATGCGT
GAATGTTTGGTCCTTTTGTTGCAATCAAGAAGAATCTTAAGAGATATGATGTATTATAGA
TTCAAAATGGATAGATTCCTTTCTGGTAATCTCTCTGTATTTGAAATTCAAAATCTATTA
CATTCACAAAGAGAAGATAAAGAATCTGATTGGATTGCTGAAATTCAAGAATTAAAACGT
AATTTAGTTAGTGAAGTTAGAAGAAATCATACATTAGAAAGAGATCTTAATCGTCTTGAT
AAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCAGACAAA
GCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAGXXXXXXXXXXTCGTGCTCTTC
CAACTCTTCCAGAACAAAAAGACCAATCTGATGAACCACCCAATCTTGTCGATGTTTTAA
ACAAGGCTCGTCAAGCTGAACCATCTTTGGAGCCAGAAATCAAAAAGATCCTTGATAACC
TCAAAAAACTTGAAGAATATAATCTCACCACCTCTGCCGATAATTATTCCTCTTTCCTCA
AGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGAAATTCGTGAACAACAAAAGAAGGAGA
AACAAAGATTAACCACCTCTTTGAATAATCTTCGTAAACATCAAAAATATCTCAACGAAC
AAATCGCTCAATACAATCAATACCTCCAAGATTGTCGTCTCAAACATTATCAAAACAAGA
GCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGTTGGTCCTTTCAAATTCAGTTTCAGCG
AACTTCACAAGAAAGGTGTCATCGTCGACTCTGAAGTTCCTCAAATCACCAGAAAGAAGA
TTAAATTTGTCATTAGCTCTGATACCGTTGGTGTGTTTGATGTCTCTGCTAAAATGGCTG
GTATCGATGTTCAAACTATGCGTTTGGAACTTGATGACCTTTTAGAACTCAATTCAATTG
GCACCACAACTTTAGAATTAGATCAAATCACTTTAGATGTTAATATGACCATACATTTAT
TAAACAAACTTTTCTTATATTAGTCAACTTAATAAATAAAAAATAAATAAAAAAATATTG
TGTATTAAAGAATAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
n*fyfnikliilyik*KNKIKMNKEEYSDISDSESEEVHETNNHNEHEHEEEDDTPEIVV
PERKFLKEDEDYSVPFPVMRECLVLLLQSRRILRDMMYYRFKMDRFLSGNLSVFEIQNLL
HSQREDKESDWIAEIQELKRNLVSEVRRNHTLERDLNRLDKRIALLIKNRGNIQDVLADK
AGLKAPKHKGDQK---

---RALPTLPEQKDQSDEPPNLVDVLNKARQAEPSLEPEIKKILDNLKKLEEYNLTTSAD
NYSSFLKAVALEVVNRAEIREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRL
KHYQNKSKKKKKGDGAKVGPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFD
VSAKMAGIDVQTMRLELDDLLELNSIGTTTLELDQITLDVNMTIHLLNKLFLY*st**ik
nk*knivy*rik
Translated Amino Acid sequence (All Frames) 
Frame A:
n*fyfnikliilyik*KNKIKMNKEEYSDISDSESEEVHETNNHNEHEHEEEDDTPEIVV
PERKFLKEDEDYSVPFPVMRECLVLLLQSRRILRDMMYYRFKMDRFLSGNLSVFEIQNLL
HSQREDKESDWIAEIQELKRNLVSEVRRNHTLERDLNRLDKRIALLIKNRGNIQDVLADK
AGLKAPKHKGDQK---

---scssnssrtkrpi**ttqscrcfkqgsss*tifgarnqkdp**pqkt*ri*shhlcr
*lflfpqgsrtrss*sc*ns*ttkegetkinhlfe*ss*tskisqrtnrsiqsipprlss
qtlskqeqkeeegrwcqswsfqiqfqrtsqerchrrl*sssnhqked*ich*l*yrwcv*
clc*ngwyrcsnyafgt**pfrtqfnwhhnfrirsnhfrc*ydhtfikqtflilvnlink
k*ikkycvlknkk

Frame B:
infili*ns*fyi*nkkik*k*tkkniqifqtlnqrkfmkqtiimntntkkkmthqklly
qnvns*kkmkiiqfhsqscvnvwsfccnqees*ei*ciidskwidsflvislylkfkiyy
ihkekiknligllkfkn*nvi*lvkleeiih*keilivlirelhf*lkivvifkmy*qtk
lvlkhqnireik---

---RALPTLPEQKDQSDEPPNLVDVLNKARQAEPSLEPEIKKILDNLKKLEEYNLTTSAD
NYSSFLKAVALEVVNRAEIREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRL
KHYQNKSKKKKKGDGAKVGPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFD
VSAKMAGIDVQTMRLELDDLLELNSIGTTTLELDQITLDVNMTIHLLNKLFLY*st**ik
nk*knivy*rik

Frame C:
lilf*ykthnfiykikk*nkneqrrifryfrl*irgss*nkqs**trtrrrr*htrncct
rt*ilkrr*rlfssipsha*mfgpfvaikknlkrydvl*iqng*ipfw*slci*nsksit
ftkrr*ri*ldc*nsrikt*fs**s*kksyirkrs*ss**enctfn*ksw*ysrcisrqs
ws*stkt*grsk---

---vlfqlfqnkktnlmnhpilsmf*trlvklnhlwsqkskrslitsknlkniispplpi
iiplssrqshsk*livlkfvnnkrrrnkd*ppl*iifvniknistnkslntintskivvs
niiktrakrrrremvpklvlsnsvsanftrkvssstlkflksperrlnlslaliplvclm
sllkwlvsmfklcvwnlmtf*nsiqlapql*n*iksl*mli*pyiy*tnfsyisqlnk*k
inkkilcike*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP536 (VHP536Q) /CSM/VH/VHP5-B/VHP536Q.Seq.d/                       2369   0.0
CHM205 (CHM205Q) /CSM/CH/CHM2-A/CHM205Q.Seq.d/                       1380   0.0
AHN241 (AHN241Q) /CSM/AH/AHN2-B/AHN241Q.Seq.d/                       1380   0.0
AHB795 (AHB795Q) /CSM/AH/AHB7-D/AHB795Q.Seq.d/                       1380   0.0
SHL120 (SHL120Q) /CSM/SH/SHL1-A/SHL120Q.Seq.d/                       1378   0.0
SHK836 (SHK836Q) /CSM/SH/SHK8-B/SHK836Q.Seq.d/                       1370   0.0
AHE179 (AHE179Q) /CSM/AH/AHE1-D/AHE179Q.Seq.d/                       1368   0.0
SHA237 (SHA237Q) /CSM/SH/SHA2-B/SHA237Q.Seq.d/                       1366   0.0
AHL870 (AHL870Q) /CSM/AH/AHL8-C/AHL870Q.Seq.d/                       1366   0.0
AHE493 (AHE493Q) /CSM/AH/AHE4-D/AHE493Q.Seq.d/                       1364   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446308) Dictyostelium discoideum cDNA clone:ddv62g09, 3' ...  1388   0.0    1
(U81156) Dictyostelium discoideum RasGAP1 (rgaA) gene, compl...  1380   0.0    1
(L75794) Dictyostelium discoideum RasGTPase-activating prote...  1380   0.0    1
(BJ382859) Dictyostelium discoideum cDNA clone:ddc46i02, 3' ...  1380   0.0    1
(BJ355482) Dictyostelium discoideum cDNA clone:dda57a12, 3' ...  1380   0.0    1
(BJ348965) Dictyostelium discoideum cDNA clone:dda34n23, 3' ...  1380   0.0    1
(BJ408515) Dictyostelium discoideum cDNA clone:dds46g05, 3' ...  1378   0.0    1
(BJ408292) Dictyostelium discoideum cDNA clone:dds45h10, 3' ...  1370   0.0    1
(BJ350453) Dictyostelium discoideum cDNA clone:dda39m19, 3' ...  1368   0.0    1
(BJ350443) Dictyostelium discoideum cDNA clone:dda39j24, 3' ...  1364   0.0    1
dna update 2008.12.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U81156_1(U81156|pid:none) Dictyostelium discoideum RasGAP1 (rgaA...   402   e-110
AK302981_1(AK302981|pid:none) Homo sapiens cDNA FLJ52376 complet...   102   3e-20
AK303054_1(AK303054|pid:none) Homo sapiens cDNA FLJ50398 complet...   102   3e-20
(Q13576) RecName: Full=Ras GTPase-activating-like protein IQGAP2...   102   3e-20
AY351902_1(AY351902|pid:none) Homo sapiens testis specific IQ mo...   102   3e-20
BT072767_1(BT072767|pid:none) Salmo salar clone ssal-rgf-539-307...    99   3e-19
AB076897_1(AB076897|pid:none) Ciona intestinalis Ci-IQGAP mRNA f...    99   5e-19
BC150958_1(BC150958|pid:none) Mus musculus IQ motif containing G...    94   1e-17
(Q3UQ44) RecName: Full=Ras GTPase-activating-like protein IQGAP2;      94   1e-17
AB093561_1(AB093561|pid:none) Xenopus laevis XIQGAP2 mRNA for IQ...    92   3e-17
protein update 2009. 7.21
PSORT 

 psg: 0.52  gvh: 0.02  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.18  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for VHP536 is nuc
5' end seq. ID VHP536F
5' end seq. 
>VHP536F.Seq
AATTAATTTTATTTTAATATAAAACTCATAATTTTATATATAAAATAAAAAAATAAAATA
AAAATGAACAAAGAAGAATATTCAGATATTTCAGACTCTGAATCAGAGGAAGTTCATGAA
ACAAACAATCATAATGAACACGAACACGAAGAAGAAGATGACACACCAGAAATTGTTGTA
CCAGAACGTAAATTCTTAAAAGAAGATGAAGATTATTCAGTTCCATTCCCAGTCATGCGT
GAATGTTTGGTCCTTTTGTTGCAATCAAGAAGAATCTTAAGAGATATGATGTATTATAGA
TTCAAAATGGATAGATTCCTTTCTGGTAATCTCTCTGTATTTGAAATTCAAAATCTATTA
CATTCACAAAGAGAAGATAAAGAATCTGATTGGATTGCTGAAATTCAAGAATTAAAACGT
AATTTAGTTAGTGAAGTTAGAAGAAATCATACATTAGAAAGAGATCTTAATCGTCTTGAT
AAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCAGACAAA
GCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAGNNNNNNNNNN
Length of 5' end seq. 589
3' end seq. ID VHP536Z
3' end seq. 
>VHP536Z.Seq
NNNNNNNNNNTCGTGCTCTTCCAACTCTTCCAGAACAAAAAGACCAATCTGATGAACCAC
CCAATCTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGAACCATCTTTGGAGCCAGAAA
TCAAAAAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTCACCACCTCTGCCG
ATAATTATTCCTCTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGAAATTC
GTGAACAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAATAATCTTCGTAAAC
ATCAAAAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTCCAAGATTGTCGTC
TCAAACATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGTTGGTC
CTTTCAAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTCGACTCTGAAGTTC
CTCAAATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACCGTTGGTGTGTTTG
ATGTCTCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTGGAACTTGATGACC
TTTTAGAACTCAATTCAATTGGCACCACAACTTTAGAATTAGATCAAATCACTTTAGATG
TTAATATGACCATACATTTATTAAACAAACTTTTCTTATATTAGTCAACTTAATAAATAA
AAAATAAATAAAAAAATATTGTGTATTAAAGAATAAAAAA
Length of 3' end seq. 760
Connected seq. ID VHP536P
Connected seq. 
>VHP536P.Seq
AATTAATTTTATTTTAATATAAAACTCATAATTTTATATATAAAATAAAAAAATAAAATA
AAAATGAACAAAGAAGAATATTCAGATATTTCAGACTCTGAATCAGAGGAAGTTCATGAA
ACAAACAATCATAATGAACACGAACACGAAGAAGAAGATGACACACCAGAAATTGTTGTA
CCAGAACGTAAATTCTTAAAAGAAGATGAAGATTATTCAGTTCCATTCCCAGTCATGCGT
GAATGTTTGGTCCTTTTGTTGCAATCAAGAAGAATCTTAAGAGATATGATGTATTATAGA
TTCAAAATGGATAGATTCCTTTCTGGTAATCTCTCTGTATTTGAAATTCAAAATCTATTA
CATTCACAAAGAGAAGATAAAGAATCTGATTGGATTGCTGAAATTCAAGAATTAAAACGT
AATTTAGTTAGTGAAGTTAGAAGAAATCATACATTAGAAAGAGATCTTAATCGTCTTGAT
AAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCAGACAAA
GCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAG----------TCGTGCTCTTC
CAACTCTTCCAGAACAAAAAGACCAATCTGATGAACCACCCAATCTTGTCGATGTTTTAA
ACAAGGCTCGTCAAGCTGAACCATCTTTGGAGCCAGAAATCAAAAAGATCCTTGATAACC
TCAAAAAACTTGAAGAATATAATCTCACCACCTCTGCCGATAATTATTCCTCTTTCCTCA
AGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGAAATTCGTGAACAACAAAAGAAGGAGA
AACAAAGATTAACCACCTCTTTGAATAATCTTCGTAAACATCAAAAATATCTCAACGAAC
AAATCGCTCAATACAATCAATACCTCCAAGATTGTCGTCTCAAACATTATCAAAACAAGA
GCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGTTGGTCCTTTCAAATTCAGTTTCAGCG
AACTTCACAAGAAAGGTGTCATCGTCGACTCTGAAGTTCCTCAAATCACCAGAAAGAAGA
TTAAATTTGTCATTAGCTCTGATACCGTTGGTGTGTTTGATGTCTCTGCTAAAATGGCTG
GTATCGATGTTCAAACTATGCGTTTGGAACTTGATGACCTTTTAGAACTCAATTCAATTG
GCACCACAACTTTAGAATTAGATCAAATCACTTTAGATGTTAATATGACCATACATTTAT
TAAACAAACTTTTCTTATATTAGTCAACTTAATAAATAAAAAATAAATAAAAAAATATTG
TGTATTAAAGAATAAAAAA
Length of connected seq. 1329
Full length Seq ID -
Full length Seq. -
Length of full length seq. -