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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP533
Library VH
(Link to library)
Clone ID VHP533
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16251-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHP533 (VHP533Q) /CSM/VH/VHP5-B/VHP533Q.Seq.d/
TTTTGTTCAAAAGATTTTCAAAGAGCCAAAATTGTTATCACCATCAAATAATTACGAAAA
GGATACAAATAATTTATATTTGAATACAAATAGTAATTTAGATTTTTCATTCCAATGCAA
AGAGAAATTACCACATGGTTCCGTCGAAATTAAAGTTATGTCAACTGGTATCAACTATAA
AGATAATCTTTTCTATAGAGGTTTATTACCACAAGAAATCTTTACAAAAGGTGATATTTA
TTCTCCACCATTCGGTTTAGAATGTGCTGGTTATATTACACGTGTTGCACCAAGTGGCGT
TACTCGTTTCAAAGTTGGTGATCAAGTTGTTGGTTTTGCAAGCCATTCTTTAAGTTCACT
AGCTATTACTCATCAAGATAAAATTGTATTAAAACCTGAAAATATTTCATTCAATGAAGC
TGCTGCCGTTTGTGTAGTTTATGCAACAAGTTATTATTCAATTTTCCATATTGGTGCATT
TATGGCCGATAAAGAATCAATCCTAGTTCATTCTGCAACTGGTGGTGTTGGTTTAGCAAC
TTTAAATCTTTTAAAATGGAAGAGAAATCAATTGAAAAAACATGGCAACTCTGAAATTAG
TAATGATGCTTCAATTTATGCTACAGTGGGTTCTAAAGXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
FVQKIFKEPKLLSPSNNYEKDTNNLYLNTNSNLDFSFQCKEKLPHGSVEIKVMSTGINYK
DNLFYRGLLPQEIFTKGDIYSPPFGLECAGYITRVAPSGVTRFKVGDQVVGFASHSLSSL
AITHQDKIVLKPENISFNEAAAVCVVYATSYYSIFHIGAFMADKESILVHSATGGVGLAT
LNLLKWKRNQLKKHGNSEISNDASIYATVGSK---
Translated Amino Acid sequence (All Frames) 
Frame A:
fcskdfqrakivitik*lrkgyk*fifeyk**frffipmqreittwfrrn*syvnwyql*
r*sfl*rfittrnlykr*ylfstirfrmcwlyytcctkwrysfqsw*sscwfckpffkft
syyssr*ncikt*kyfiq*sccrlcslcnkllfnfpywciygr*rinpssfcnwwcwfsn
fksfkmeeksiektwql*n***cfnlcysgf*---


Frame B:
FVQKIFKEPKLLSPSNNYEKDTNNLYLNTNSNLDFSFQCKEKLPHGSVEIKVMSTGINYK
DNLFYRGLLPQEIFTKGDIYSPPFGLECAGYITRVAPSGVTRFKVGDQVVGFASHSLSSL
AITHQDKIVLKPENISFNEAAAVCVVYATSYYSIFHIGAFMADKESILVHSATGGVGLAT
LNLLKWKRNQLKKHGNSEISNDASIYATVGSK---


Frame C:
lfkrfsksqncyhhqiitkriqiiyi*iqivi*ifhsnakrnyhmvpsklklcqlvstik
iifsievyyhkkslqkvifilhhsv*nvlvilhvlhqvallvsklvikllvlqail*vh*
lllikikly*nlkifhsmkllpfv*fmqqviiqfsilvhlwpiknqs*filqlvvlv*ql
*if*ngrein*knmatlklvmmlqfmlqwvl---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP533 (VHP533Q) /CSM/VH/VHP5-B/VHP533Q.Seq.d/                       1265   0.0
VHE329 (VHE329Q) /CSM/VH/VHE3-B/VHE329Q.Seq.d/                       1172   0.0
VHL883 (VHL883Q) /CSM/VH/VHL8-D/VHL883Q.Seq.d/                       1154   0.0
VHH442 (VHH442Q) /CSM/VH/VHH4-B/VHH442Q.Seq.d/                       1031   0.0
VHO116 (VHO116Q) /CSM/VH/VHO1-A/VHO116Q.Seq.d/                       1015   0.0
VHI375 (VHI375Q) /CSM/VH/VHI3-D/VHI375Q.Seq.d/                        971   0.0
VHD848 (VHD848Q) /CSM/VH/VHD8-B/VHD848Q.Seq.d/                        961   0.0
VHD102 (VHD102Q) /CSM/VH/VHD1-A/VHD102Q.Seq.d/                        934   0.0
VHG573 (VHG573Q) /CSM/VH/VHG5-D/VHG573Q.Seq.d/                        904   0.0
VHM773 (VHM773Q) /CSM/VH/VHM7-D/VHM773Q.Seq.d/                        890   0.0
own update 2004. 8. 5
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1263   0.0    1
(BJ427375) Dictyostelium discoideum cDNA clone:ddv62a09, 5' ...  1263   0.0    1
(BJ420458) Dictyostelium discoideum cDNA clone:ddv39j07, 5' ...  1170   0.0    1
(BJ425156) Dictyostelium discoideum cDNA clone:ddv54f22, 5' ...  1150   0.0    1
(BJ422233) Dictyostelium discoideum cDNA clone:ddv45d12, 5' ...  1031   0.0    1
(BJ426491) Dictyostelium discoideum cDNA clone:ddv59o03, 5' ...  1015   0.0    1
(BJ423018) Dictyostelium discoideum cDNA clone:ddv47f19, 5' ...   971   0.0    1
(BJ420173) Dictyostelium discoideum cDNA clone:ddv38p12, 5' ...   961   0.0    1
(BJ419727) Dictyostelium discoideum cDNA clone:ddv37c01, 5' ...   934   0.0    1
(BJ422068) Dictyostelium discoideum cDNA clone:ddv44a19, 5' ...   902   0.0    1
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W9) RecName: Full=Probable polyketide synthase 16;         ...   399   e-110
(Q869X2) RecName: Full=Probable polyketide synthase 17;         ...   372   e-102
AC116963_19(AC116963|pid:none) Dictyostelium discoideum chromoso...   147   3e-34
(Q54B49) RecName: Full=Probable polyketide synthase 45;         ...   146   6e-34
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   145   1e-33
(Q55DM7) RecName: Full=Probable polyketide synthase 2;          ...   144   2e-33
AC116963_16(AC116963|pid:none) Dictyostelium discoideum chromoso...   142   1e-32
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   136   6e-31
(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   135   8e-31
(Q54QD1) RecName: Full=Probable polyketide synthase 23;         ...   115   1e-24
protein update 2009. 7.21
PSORT 

 psg: 0.72  gvh: 0.27  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHP533 is cyt
5' end seq. ID VHP533F
5' end seq. 
>VHP533F.Seq
TTTTGTTCAAAAGATTTTCAAAGAGCCAAAATTGTTATCACCATCAAATAATTACGAAAA
GGATACAAATAATTTATATTTGAATACAAATAGTAATTTAGATTTTTCATTCCAATGCAA
AGAGAAATTACCACATGGTTCCGTCGAAATTAAAGTTATGTCAACTGGTATCAACTATAA
AGATAATCTTTTCTATAGAGGTTTATTACCACAAGAAATCTTTACAAAAGGTGATATTTA
TTCTCCACCATTCGGTTTAGAATGTGCTGGTTATATTACACGTGTTGCACCAAGTGGCGT
TACTCGTTTCAAAGTTGGTGATCAAGTTGTTGGTTTTGCAAGCCATTCTTTAAGTTCACT
AGCTATTACTCATCAAGATAAAATTGTATTAAAACCTGAAAATATTTCATTCAATGAAGC
TGCTGCCGTTTGTGTAGTTTATGCAACAAGTTATTATTCAATTTTCCATATTGGTGCATT
TATGGCCGATAAAGAATCAATCCTAGTTCATTCTGCAACTGGTGGTGTTGGTTTAGCAAC
TTTAAATCTTTTAAAATGGAAGAGAAATCAATTGAAAAAACATGGCAACTCTGAAATTAG
TAATGATGCTTCAATTTATGCTACAGTGGGTTCTAAAGNNNNNNNNNN
Length of 5' end seq. 648
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -