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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP531
Library VH
(Link to library)
Clone ID VHP531
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12899-1
Original site URL
Representative seq. ID VHP531P
(Link to Original site)
Representative DNA sequence 
>VHP531 (VHP531Q) /CSM/VH/VHP5-B/VHP531Q.Seq.d/
GTACAACCCTAATGGATAGCACATTGGCAATTGGATTAACTGGTGCCAATAAATGGCGTC
GTATTAAAGGTCAAGATCGTAGATTGGATAAGGTTGATGATGTGAAAATGAAGATGGAGT
GGCATATGGACGATAACCAATTTCAACAGAAAATCAAGGACACTCAAGTACTCGTCACTA
AAGATTACCAAAAATGGTCATGGGAATTAATGTTTGAATTGTTGGAAGGACCTTTGAACA
ATCCTCAACATTTATCAAATACATTGAAAACCAAATTCATTAAAAGAATCTTATCATTTC
TACGTCCAAATAAGAAACTATTCTCAACAATGGCTTGGACAACTGAAAATTTAAAATATG
TTCGTACTGCTTGTGTAGCATTGGAAGTTTTAATCTCTCATGAAATTGGTTTCGATTTTC
TTAAAGATAATAAAACTATAATTCAAATCGCCGATATGTTAAAAGTGGAATTAGATTATA
ATATTAAACCACCACCATCATCATCCTCATCATCAGAAAATAAAAAAGATAATGTACGTT
TATTGAATCCAGAAAAAGTTTTAAAAACAATGTCTCGTGAATACTTTACAATGTTGGGTA
CATTGAGTAGTAATTTATTAGGTTTGGAAATATTAGCACGTAATAACATTTTCGATTATA
TTAAACCATTGGCTGAATTGCXXXXXXXXXXTGAATTAGCAAAAACTGAAAAAGGTTGTC
AATTAATTAGAAAATCAAATAACTATCAAAGATTTTTAAAAATCATTCAAGATCCAACCG
CTAAACAATTGGATAAAAGAGCATCGTTAATCGCAATTGGTCATATTGGTTCTTCTGTCG
ATGGTTATTCATTCGTTAAAGAAGGTGATACAATCAAACTATTGATTGGAATAGCAGAGA
AATCTCAATGTTTGGCATTACGTAGTACTTGTTTCTATGCTTTGGGTATGATTTCATGTA
TTGAAGAAGCTCAACCAATCTTTAATTCCTTTGGTTGGGAATCACCATCAGATTTAAACT
CTAGAATCTTGTTACCAAAGGATTTAAAGAATTCAACTTTCCTAAGTGTACCACAATATC
AATATCAAGGTAGTTGGGCTGATCATTCATTTGAAACTTTACCATCAAATCATTTCTCTG
ATCCAATTAAAAATGAAATCATTTCTTTTGTTGGTAATTTATCTTCACATATCACTGCTG
AAGGTGCTTCAAAGAATTTAAAACGTTTAAAAATCAAATATCCTGATCATTTTGCAACAA
GTGAAATTTTAAATGCAGTTTTCATTTTATTAAATACTTTTAAATATCGTTTAGGTGCTA
GAAGATCTCATCTTATGATTTATTTGATGTTGCAATATTTTCATNTNATCCATATCANCA
TNTAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
TTLMDSTLAIGLTGANKWRRIKGQDRRLDKVDDVKMKMEWHMDDNQFQQKIKDTQVLVTK
DYQKWSWELMFELLEGPLNNPQHLSNTLKTKFIKRILSFLRPNKKLFSTMAWTTENLKYV
RTACVALEVLISHEIGFDFLKDNKTIIQIADMLKVELDYNIKPPPSSSSSSENKKDNVRL
LNPEKVLKTMSREYFTMLGTLSSNLLGLEILARNNIFDYIKPLAEL---

---ELAKTEKGCQLIRKSNNYQRFLKIIQDPTAKQLDKRASLIAIGHIGSSVDGYSFVKE
GDTIKLLIGIAEKSQCLALRSTCFYALGMISCIEEAQPIFNSFGWESPSDLNSRILLPKD
LKNSTFLSVPQYQYQGSWADHSFETLPSNHFSDPIKNEIISFVGNLSSHITAEGASKNLK
RLKIKYPDHFATSEILNAVFILLNTFKYRLGARRSHLMIYLMLQYFHXIHIXX*
Translated Amino Acid sequence (All Frames) 
Frame A:
vqp*wiahwqld*lvpingvvlkvkivdwirlmm*k*rwsgiwtitnfnrksrtlkyssl
kitknghgn*clncwkdl*tilniyqih*kpnslkesyhfyvqirnysqqwlgqlki*nm
fvllv*hwkf*slmklvsiflkiikl*fkspic*kwn*iiilnhhhhhphhqkikkimyv
y*iqkkf*kqclvntlqcwvh*vviy*vwky*hvitfsiilnhwlnc---

---*iskn*krlsin*kik*lskifknhsrsnr*tig*ksivnrnwsywffcrwlfir*r
r*ynqtidwnsreismfgit*ylflcfgydfmy*rsstnl*flwlgitirfkl*nlvtkg
fkefnfpkcttisisr*lg*sfi*nftiksfl*sn*k*nhffcw*fiftyhc*rcfkefk
tfknqis*sfcnk*nfkcsfhfikyf*isfrc*kissydlfdvaifsxxpyxhxn

Frame B:
ynpng*hignwinwcq*masy*rsrs*ig*g**cenedgvaygr*pistenqghsstrh*
rlpkmvmginv*ivgrtfeqsstfikyienqih*knliistsk*etilnngldn*kfkic
syclcsigsfnls*nwfrfs*r**nynsnrryvksgirl*y*tttiiiliirk*kr*ctf
iesrksfknnvs*ilynvgyie**firfgnist**hfrly*tig*i---

---ELAKTEKGCQLIRKSNNYQRFLKIIQDPTAKQLDKRASLIAIGHIGSSVDGYSFVKE
GDTIKLLIGIAEKSQCLALRSTCFYALGMISCIEEAQPIFNSFGWESPSDLNSRILLPKD
LKNSTFLSVPQYQYQGSWADHSFETLPSNHFSDPIKNEIISFVGNLSSHITAEGASKNLK
RLKIKYPDHFATSEILNAVFILLNTFKYRLGARRSHLMIYLMLQYFHXIHIXX*

Frame C:
TTLMDSTLAIGLTGANKWRRIKGQDRRLDKVDDVKMKMEWHMDDNQFQQKIKDTQVLVTK
DYQKWSWELMFELLEGPLNNPQHLSNTLKTKFIKRILSFLRPNKKLFSTMAWTTENLKYV
RTACVALEVLISHEIGFDFLKDNKTIIQIADMLKVELDYNIKPPPSSSSSSENKKDNVRL
LNPEKVLKTMSREYFTMLGTLSSNLLGLEILARNNIFDYIKPLAEL---

---n*qklkkvvn*lenqitikdf*ksfkiqplnnwikehr*sqlvilvllsmvihslkk
viqsny*le*qrnlnvwhyvvlvsmlwv*fhvlkklnqsliplvgnhhqi*tlescyqri
*riqls*vyhninikvvgliihlklyhqiisliqlkmksflllviylhisllkvlqri*n
v*ksniliilqqvkf*mqfsfy*illniv*vledlil*fi*ccnifxxsisxxk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP531 (VHP531Q) /CSM/VH/VHP5-B/VHP531Q.Seq.d/                       2666   0.0
VHP595 (VHP595Q) /CSM/VH/VHP5-D/VHP595Q.Seq.d/                       1304   0.0
SHF605 (SHF605Q) /CSM/SH/SHF6-A/SHF605Q.Seq.d/                       1302   0.0
SHD374 (SHD374Q) /CSM/SH/SHD3-D/SHD374Q.Seq.d/                       1257   0.0
CHA228 (CHA228Q) /CSM/CH/CHA2-B/CHA228Q.Seq.d/                       1255   0.0
SFF264 (SFF264Q) /CSM/SF/SFF2-C/SFF264Q.Seq.d/                       1251   0.0
VFK167 (VFK167Q) /CSM/VF/VFK1-C/VFK167Q.Seq.d/                       1235   0.0
AHB329 (AHB329Q) /CSM/AH/AHB3-B/AHB329Q.Seq.d/                       1219   0.0
AFE661 (AFE661Q) /CSM/AF/AFE6-C/AFE661Q.Seq.d/                       1195   0.0
SSB345 (SSB345Q) /CSM/SS/SSB3-B/SSB345Q.Seq.d/                        617   e-175
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446340) Dictyostelium discoideum cDNA clone:ddv62m07, 3' ...  1356   0.0    1
(AF085194) Dictyostelium discoideum cytosolic regulator pian...  1350   0.0    1
(AF080675) Dictyostelium discoideum developmental protein (D...  1350   0.0    1
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...  1350   0.0    1
(BJ427435) Dictyostelium discoideum cDNA clone:ddv62m07, 5' ...  1350   0.0    1
(BJ446501) Dictyostelium discoideum cDNA clone:ddv62m23, 3' ...  1251   0.0    2
(BJ409554) Dictyostelium discoideum cDNA clone:dds42b18, 3' ...  1251   0.0    2
(BJ398261) Dictyostelium discoideum cDNA clone:dds11o16, 3' ...  1251   0.0    1
(BJ406388) Dictyostelium discoideum cDNA clone:dds35i02, 3' ...  1243   0.0    2
(BJ377028) Dictyostelium discoideum cDNA clone:ddc22g08, 3' ...  1241   0.0    2
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF085194_1(AF085194|pid:none) Dictyostelium discoideum cytosolic...   434   e-120
AC117081_19(AC117081|pid:none) Dictyostelium discoideum chromoso...   434   e-120
AF080675_1(AF080675|pid:none) Dictyostelium discoideum developme...   434   e-120
JC5984(JC5984)cAMP-dependent cell aggregation protein - slime mo...   431   e-119
(Q09743) RecName: Full=Protein ste16;  &AJ223984_1(AJ223984|pid:...   122   2e-26
CR382132_43(CR382132|pid:none) Yarrowia lipolytica strain CLIB12...   122   3e-26
AM920437_2067(AM920437|pid:none) Penicillium chrysogenum Wiscons...   117   8e-25
AP007171_503(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...   117   1e-24
FN392322_395(FN392322|pid:none) Pichia pastoris GS115 chromosome...   108   5e-22
FM992695_669(FM992695|pid:none) Candida dubliniensis CD36 chromo...    99   4e-19
protein update 2009. 7.21
PSORT 

 psg: 0.75  gvh: 0.54  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHP531 is nuc
5' end seq. ID VHP531F
5' end seq. 
>VHP531F.Seq
GTACAACCCTAATGGATAGCACATTGGCAATTGGATTAACTGGTGCCAATAAATGGCGTC
GTATTAAAGGTCAAGATCGTAGATTGGATAAGGTTGATGATGTGAAAATGAAGATGGAGT
GGCATATGGACGATAACCAATTTCAACAGAAAATCAAGGACACTCAAGTACTCGTCACTA
AAGATTACCAAAAATGGTCATGGGAATTAATGTTTGAATTGTTGGAAGGACCTTTGAACA
ATCCTCAACATTTATCAAATACATTGAAAACCAAATTCATTAAAAGAATCTTATCATTTC
TACGTCCAAATAAGAAACTATTCTCAACAATGGCTTGGACAACTGAAAATTTAAAATATG
TTCGTACTGCTTGTGTAGCATTGGAAGTTTTAATCTCTCATGAAATTGGTTTCGATTTTC
TTAAAGATAATAAAACTATAATTCAAATCGCCGATATGTTAAAAGTGGAATTAGATTATA
ATATTAAACCACCACCATCATCATCCTCATCATCAGAAAATAAAAAAGATAATGTACGTT
TATTGAATCCAGAAAAAGTTTTAAAAACAATGTCTCGTGAATACTTTACAATGTTGGGTA
CATTGAGTAGTAATTTATTAGGTTTGGAAATATTAGCACGTAATAACATTTTCGATTATA
TTAAACCATTGGCTGAATTGCNNNNNNNNNN
Length of 5' end seq. 691
3' end seq. ID VHP531Z
3' end seq. 
>VHP531Z.Seq
NNNNNNNNNNTGAATTAGCAAAAACTGAAAAAGGTTGTCAATTAATTAGAAAATCAAATA
ACTATCAAAGATTTTTAAAAATCATTCAAGATCCAACCGCTAAACAATTGGATAAAAGAG
CATCGTTAATCGCAATTGGTCATATTGGTTCTTCTGTCGATGGTTATTCATTCGTTAAAG
AAGGTGATACAATCAAACTATTGATTGGAATAGCAGAGAAATCTCAATGTTTGGCATTAC
GTAGTACTTGTTTCTATGCTTTGGGTATGATTTCATGTATTGAAGAAGCTCAACCAATCT
TTAATTCCTTTGGTTGGGAATCACCATCAGATTTAAACTCTAGAATCTTGTTACCAAAGG
ATTTAAAGAATTCAACTTTCCTAAGTGTACCACAATATCAATATCAAGGTAGTTGGGCTG
ATCATTCATTTGAAACTTTACCATCAAATCATTTCTCTGATCCAATTAAAAATGAAATCA
TTTCTTTTGTTGGTAATTTATCTTCACATATCACTGCTGAAGGTGCTTCAAAGAATTTAA
AACGTTTAAAAATCAAATATCCTGATCATTTTGCAACAAGTGAAATTTTAAATGCAGTTT
TCATTTTATTAAATACTTTTAAATATCGTTTAGGTGCTAGAAGATCTCATCTTATGATTT
ATTTGATGTTGCAATATTTTCATNTNATCCATATCANCATNTAAAT
Length of 3' end seq. 706
Connected seq. ID VHP531P
Connected seq. 
>VHP531P.Seq
GTACAACCCTAATGGATAGCACATTGGCAATTGGATTAACTGGTGCCAATAAATGGCGTC
GTATTAAAGGTCAAGATCGTAGATTGGATAAGGTTGATGATGTGAAAATGAAGATGGAGT
GGCATATGGACGATAACCAATTTCAACAGAAAATCAAGGACACTCAAGTACTCGTCACTA
AAGATTACCAAAAATGGTCATGGGAATTAATGTTTGAATTGTTGGAAGGACCTTTGAACA
ATCCTCAACATTTATCAAATACATTGAAAACCAAATTCATTAAAAGAATCTTATCATTTC
TACGTCCAAATAAGAAACTATTCTCAACAATGGCTTGGACAACTGAAAATTTAAAATATG
TTCGTACTGCTTGTGTAGCATTGGAAGTTTTAATCTCTCATGAAATTGGTTTCGATTTTC
TTAAAGATAATAAAACTATAATTCAAATCGCCGATATGTTAAAAGTGGAATTAGATTATA
ATATTAAACCACCACCATCATCATCCTCATCATCAGAAAATAAAAAAGATAATGTACGTT
TATTGAATCCAGAAAAAGTTTTAAAAACAATGTCTCGTGAATACTTTACAATGTTGGGTA
CATTGAGTAGTAATTTATTAGGTTTGGAAATATTAGCACGTAATAACATTTTCGATTATA
TTAAACCATTGGCTGAATTGC----------TGAATTAGCAAAAACTGAAAAAGGTTGTC
AATTAATTAGAAAATCAAATAACTATCAAAGATTTTTAAAAATCATTCAAGATCCAACCG
CTAAACAATTGGATAAAAGAGCATCGTTAATCGCAATTGGTCATATTGGTTCTTCTGTCG
ATGGTTATTCATTCGTTAAAGAAGGTGATACAATCAAACTATTGATTGGAATAGCAGAGA
AATCTCAATGTTTGGCATTACGTAGTACTTGTTTCTATGCTTTGGGTATGATTTCATGTA
TTGAAGAAGCTCAACCAATCTTTAATTCCTTTGGTTGGGAATCACCATCAGATTTAAACT
CTAGAATCTTGTTACCAAAGGATTTAAAGAATTCAACTTTCCTAAGTGTACCACAATATC
AATATCAAGGTAGTTGGGCTGATCATTCATTTGAAACTTTACCATCAAATCATTTCTCTG
ATCCAATTAAAAATGAAATCATTTCTTTTGTTGGTAATTTATCTTCACATATCACTGCTG
AAGGTGCTTCAAAGAATTTAAAACGTTTAAAAATCAAATATCCTGATCATTTTGCAACAA
GTGAAATTTTAAATGCAGTTTTCATTTTATTAAATACTTTTAAATATCGTTTAGGTGCTA
GAAGATCTCATCTTATGATTTATTTGATGTTGCAATATTTTCATNTNATCCATATCANCA
TNTAAAT
Length of connected seq. 1377
Full length Seq ID -
Full length Seq. -
Length of full length seq. -