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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP530
Library VH
(Link to library)
Clone ID VHP530
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11514-1
Original site URL
Representative seq. ID VHP530P
(Link to Original site)
Representative DNA sequence 
>VHP530 (VHP530Q) /CSM/VH/VHP5-B/VHP530Q.Seq.d/
GATTAGAAGAAGTTGGACCAAATGCAATTCCAGATGTTAAAAGATATCCAATTTTAGAAT
TTTTATATTTTATGTGGAATCCATTATCATGGACTATGGAAGTTGCAGCTATTGTATCAA
TTGCATTATTAGATTGGGTTGATTTTATATTGATTTGTGCATTATTACTTTTGAATGCAA
CCATTGGTTTCATTGAAGAGAATACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCAT
TGGTTAGTCAAATCCGTTGTATGAGAGATGGTGAATGGGTTATGTTACCATCACCAGATC
TTGTACCAGGTGATGTTGTAATGTTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAG
TTATCGAAGCTGAACAAGTTAAAATCGATCAATCTTCATTGACAGGTGAATCTTTACCAG
TCACTAAAAAGATTGGTGATGAAGTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAA
AATGTGTTGTCACTGCAACTGGTGTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTAC
AAGAAACCGAAGGACATGGTCATCTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTA
TTAGTTTCATCGCCATTTGGGTACTCGTTGAATXXXXXXXXXXTTTGAATGATGGTACCA
TGTTAACAATCTCTAAAGATCGTGTAAGAGCTAGAAATGAACCAGATCAATGGAATCTTT
TCGAAGTATTCACTATGGCACTTTGTTATGGTTTCTATTTAGTAGGTTCAACCATTGTAT
TCTTTGCAATCATTCATGATGGTACTTGGTTCCATGATGCTATCAATCTTCGTATCTTAA
CCGATAATGAACTTCGTGGTCTCATCTATCTTCAAGTCTCTATCTCTGGTCTTGCAACTA
TTTTCGTATCACGTAGTCAAGGTTTCTCTTACTTTGAACGTCCAGGTAATCTTGTAATCT
TTGCTTTCGTTATGTCTCAAATTGTTGCTACCTTTATTGGTGTCTATGGTTTCCGTGGTT
ATCCACATGATTCCTTTTCCGATAATCCAGATTATCCAGTACATGGTACAAATTTCCAAG
GTTGTGGTTGGGGTTGGGCTGTTTGTGCTTGGATTTGGTGTTTCCTTTGGTACATTCCAA
TGGATTTCATTAAATTAGGTGTCACCTATATTCTCCGTGGTAAGATTGAACCAATCAATA
AAGATGCACTCCGTAAAATCTATGGTTGGTTTGGTAAAGAAATCCCAAAGGAAGCAACTC
AAGTCTCTCATAAAGTTGCTGAACAACAAGCTAAAAGAGATGCTTTACATGCACAAGAAA
CCCGTCATAAATCTGTTGTTACCGATAATAAAGTTTAAAATAACAATAATATTTAAATAA
TGTATAAAATAGTTTTAAAATTTTAGCAACAAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LEEVGPNAIPDVKRYPILEFLYFMWNPLSWTMEVAAIVSIALLDWVDFILICALLLLNAT
IGFIEENTAGNAVEALKNSLVSQIRCMRDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRV
IEAEQVKIDQSSLTGESLPVTKKIGDEVYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQ
ETEGHGHLQVILRNIGLFCISFIAIWVLVE---

---LNDGTMLTISKDRVRARNEPDQWNLFEVFTMALCYGFYLVGSTIVFFAIIHDGTWFH
DAINLRILTDNELRGLIYLQVSISGLATIFVSRSQGFSYFERPGNLVIFAFVMSQIVATF
IGVYGFRGYPHDSFSDNPDYPVHGTNFQGCGWGWAVCAWIWCFLWYIPMDFIKLGVTYIL
RGKIEPINKDALRKIYGWFGKEIPKEATQVSHKVAEQQAKRDALHAQETRHKSVVTDNKV
*nnnni*imykivlkf*qqi
Translated Amino Acid sequence (All Frames) 
Frame A:
d*kkldqmqfqmlkdiqf*nfyilcgihyhglwklqllyqlhy*iglily*fvhyyf*mq
plvslkriqqvmq*rl*rihwlvksvv*emvnglcyhhqilyqvml*c*rlvplfqpive
lsklnklksinlh*qvnlyqslkrlvmkftlvqq*nkvkqnvlslqlv*tlslvvlqi*y
kkpkdmvifklfleilvsfvlvsspfgysln---

---fe*wyhvnnl*rscks*k*trsmesfrsihygtllwflfsrfnhcilcnhs*wylvp
*cyqssylnr**tswshlssslylwscnyfrit*srflll*tsr*scnlcfryvsnccyl
ywclwfpwlst*flfr*srlsstwykfprlwlglgclcldlvfplvhsngfh*irchlys
pw*d*tnq*rctp*nlwlvw*rnpkgsnssls*sc*tts*krcftctrnps*iccyr**s
lk*q*ylnnv*nsfkilatn

Frame B:
irrswtkcnsrc*kisnfrififyvesiimdygscsycinciirlg*fyidlciitfecn
hwfh*reysr*csrgfkefig*snplyerw*mgyvtitrsctr*ccnvkdwchyssrl*s
yrs*ts*nrsifidr*iftsh*kdw**sllwfsnetr*skmcchcnwckhflwscckfst
rnrrtwssssys*kywslly*fhrhlgtr*---

---LNDGTMLTISKDRVRARNEPDQWNLFEVFTMALCYGFYLVGSTIVFFAIIHDGTWFH
DAINLRILTDNELRGLIYLQVSISGLATIFVSRSQGFSYFERPGNLVIFAFVMSQIVATF
IGVYGFRGYPHDSFSDNPDYPVHGTNFQGCGWGWAVCAWIWCFLWYIPMDFIKLGVTYIL
RGKIEPINKDALRKIYGWFGKEIPKEATQVSHKVAEQQAKRDALHAQETRHKSVVTDNKV
*nnnni*imykivlkf*qqi

Frame C:
LEEVGPNAIPDVKRYPILEFLYFMWNPLSWTMEVAAIVSIALLDWVDFILICALLLLNAT
IGFIEENTAGNAVEALKNSLVSQIRCMRDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRV
IEAEQVKIDQSSLTGESLPVTKKIGDEVYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQ
ETEGHGHLQVILRNIGLFCISFIAIWVLVE---

---*mmvpc*qslkiv*elemnqingifskyslwhfvmvsi**vqplyslqsfmmvlgsm
mlsifvs*pimnfvvssifkslslvlqlfsyhvvkvsltlnvqvil*sllslclklllpl
lvsmvsvvihmipfpiiqiiqymvqiskvvvgvglfvlgfgvsfgtfqwisln*vspifs
vvrlnqsikmhsvksmvglvkksqrkqlkslikllnnklkemlymhkkpvinlllpiikf
kitiifk*cik*f*nfsnk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP530 (VHP530Q) /CSM/VH/VHP5-B/VHP530Q.Seq.d/                       2605   0.0
VHJ167 (VHJ167Q) /CSM/VH/VHJ1-C/VHJ167Q.Seq.d/                       1382   0.0
VHD413 (VHD413Q) /CSM/VH/VHD4-A/VHD413Q.Seq.d/                       1382   0.0
VHB587 (VHB587Q) /CSM/VH/VHB5-D/VHB587Q.Seq.d/                       1382   0.0
VHA802 (VHA802Q) /CSM/VH/VHA8-A/VHA802Q.Seq.d/                       1382   0.0
VHA582 (VHA582Q) /CSM/VH/VHA5-D/VHA582Q.Seq.d/                       1382   0.0
VFN107 (VFN107Q) /CSM/VF/VFN1-A/VFN107Q.Seq.d/                       1382   0.0
CHR538 (CHR538Q) /CSM/CH/CHR5-B/CHR538Q.Seq.d/                       1382   0.0
CHL228 (CHL228Q) /CSM/CH/CHL2-B/CHL228Q.Seq.d/                       1382   0.0
VHC544 (VHC544Q) /CSM/VH/VHC5-B/VHC544Q.Seq.d/                       1376   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446328) Dictyostelium discoideum cDNA clone:ddv62k07, 3' ...  1390   0.0    1
(X98286) D.discoideum mRNA for P-type ATPase.                    1382   0.0    1
(BJ442333) Dictyostelium discoideum cDNA clone:ddv49e17, 3' ...  1382   0.0    1
(BJ438504) Dictyostelium discoideum cDNA clone:ddv37j04, 3' ...  1382   0.0    1
(BJ437645) Dictyostelium discoideum cDNA clone:ddv34m21, 3' ...  1382   0.0    1
(BJ436961) Dictyostelium discoideum cDNA clone:ddv32c21, 3' ...  1382   0.0    1
(BJ436716) Dictyostelium discoideum cDNA clone:ddv32d02, 3' ...  1382   0.0    1
(BJ386178) Dictyostelium discoideum cDNA clone:ddc57k09, 3' ...  1382   0.0    1
(BJ382307) Dictyostelium discoideum cDNA clone:ddc44g08, 3' ...  1382   0.0    1
(BJ438090) Dictyostelium discoideum cDNA clone:ddv36g11, 3' ...  1376   0.0    1
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T30580(T30580) P-type ATPase - slime mold (Dictyostelium discoid...   497   e-139
(P54679) RecName: Full=Probable plasma membrane ATPase;         ...   497   e-139
AL603947_1(AL603947|pid:none) Ustilago maydis gene for predicted...   241   5e-62
D88424_1(D88424|pid:none) Cyanidium caldarium mRNA for plasma me...   239   1e-61
AY344962_1(AY344962|pid:none) Phytophthora infestans plasma memb...   235   3e-60
DQ533871_1(DQ533871|pid:none) Phytophthora nicotianae plasma mem...   234   4e-60
(Q7XPY2) RecName: Full=Plasma membrane ATPase;          EC=3.6.3...   232   2e-59
FB811294_1(FB811294|pid:none) Sequence 30567 from Patent WO20080...   232   2e-59
FB811240_1(FB811240|pid:none) Sequence 30513 from Patent WO20080...   231   5e-59
S60301(S60301)H+-exporting ATPase (EC 3.6.3.6) 9, anther-specifi...   231   5e-59
protein update 2009. 7.21
PSORT 

 psg: 0.75  gvh: 0.60  alm: 0.20  top: 0.53  tms: 0.53  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 52.0 %: plasma membrane
	 36.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VHP530 is pla
5' end seq. ID VHP530F
5' end seq. 
>VHP530F.Seq
GATTAGAAGAAGTTGGACCAAATGCAATTCCAGATGTTAAAAGATATCCAATTTTAGAAT
TTTTATATTTTATGTGGAATCCATTATCATGGACTATGGAAGTTGCAGCTATTGTATCAA
TTGCATTATTAGATTGGGTTGATTTTATATTGATTTGTGCATTATTACTTTTGAATGCAA
CCATTGGTTTCATTGAAGAGAATACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCAT
TGGTTAGTCAAATCCGTTGTATGAGAGATGGTGAATGGGTTATGTTACCATCACCAGATC
TTGTACCAGGTGATGTTGTAATGTTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAG
TTATCGAAGCTGAACAAGTTAAAATCGATCAATCTTCATTGACAGGTGAATCTTTACCAG
TCACTAAAAAGATTGGTGATGAAGTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAA
AATGTGTTGTCACTGCAACTGGTGTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTAC
AAGAAACCGAAGGACATGGTCATCTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTA
TTAGTTTCATCGCCATTTGGGTACTCGTTGAATNNNNNNNNNN
Length of 5' end seq. 643
3' end seq. ID VHP530Z
3' end seq. 
>VHP530Z.Seq
NNNNNNNNNNTTTGAATGATGGTACCATGTTAACAATCTCTAAAGATCGTGTAAGAGCTA
GAAATGAACCAGATCAATGGAATCTTTTCGAAGTATTCACTATGGCACTTTGTTATGGTT
TCTATTTAGTAGGTTCAACCATTGTATTCTTTGCAATCATTCATGATGGTACTTGGTTCC
ATGATGCTATCAATCTTCGTATCTTAACCGATAATGAACTTCGTGGTCTCATCTATCTTC
AAGTCTCTATCTCTGGTCTTGCAACTATTTTCGTATCACGTAGTCAAGGTTTCTCTTACT
TTGAACGTCCAGGTAATCTTGTAATCTTTGCTTTCGTTATGTCTCAAATTGTTGCTACCT
TTATTGGTGTCTATGGTTTCCGTGGTTATCCACATGATTCCTTTTCCGATAATCCAGATT
ATCCAGTACATGGTACAAATTTCCAAGGTTGTGGTTGGGGTTGGGCTGTTTGTGCTTGGA
TTTGGTGTTTCCTTTGGTACATTCCAATGGATTTCATTAAATTAGGTGTCACCTATATTC
TCCGTGGTAAGATTGAACCAATCAATAAAGATGCACTCCGTAAAATCTATGGTTGGTTTG
GTAAAGAAATCCCAAAGGAAGCAACTCAAGTCTCTCATAAAGTTGCTGAACAACAAGCTA
AAAGAGATGCTTTACATGCACAAGAAACCCGTCATAAATCTGTTGTTACCGATAATAAAG
TTTAAAATAACAATAATATTTAAATAATGTATAAAATAGTTTTAAAATTTTAGCAACAAA
TAA
Length of 3' end seq. 783
Connected seq. ID VHP530P
Connected seq. 
>VHP530P.Seq
GATTAGAAGAAGTTGGACCAAATGCAATTCCAGATGTTAAAAGATATCCAATTTTAGAAT
TTTTATATTTTATGTGGAATCCATTATCATGGACTATGGAAGTTGCAGCTATTGTATCAA
TTGCATTATTAGATTGGGTTGATTTTATATTGATTTGTGCATTATTACTTTTGAATGCAA
CCATTGGTTTCATTGAAGAGAATACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCAT
TGGTTAGTCAAATCCGTTGTATGAGAGATGGTGAATGGGTTATGTTACCATCACCAGATC
TTGTACCAGGTGATGTTGTAATGTTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAG
TTATCGAAGCTGAACAAGTTAAAATCGATCAATCTTCATTGACAGGTGAATCTTTACCAG
TCACTAAAAAGATTGGTGATGAAGTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAA
AATGTGTTGTCACTGCAACTGGTGTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTAC
AAGAAACCGAAGGACATGGTCATCTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTA
TTAGTTTCATCGCCATTTGGGTACTCGTTGAAT----------TTTGAATGATGGTACCA
TGTTAACAATCTCTAAAGATCGTGTAAGAGCTAGAAATGAACCAGATCAATGGAATCTTT
TCGAAGTATTCACTATGGCACTTTGTTATGGTTTCTATTTAGTAGGTTCAACCATTGTAT
TCTTTGCAATCATTCATGATGGTACTTGGTTCCATGATGCTATCAATCTTCGTATCTTAA
CCGATAATGAACTTCGTGGTCTCATCTATCTTCAAGTCTCTATCTCTGGTCTTGCAACTA
TTTTCGTATCACGTAGTCAAGGTTTCTCTTACTTTGAACGTCCAGGTAATCTTGTAATCT
TTGCTTTCGTTATGTCTCAAATTGTTGCTACCTTTATTGGTGTCTATGGTTTCCGTGGTT
ATCCACATGATTCCTTTTCCGATAATCCAGATTATCCAGTACATGGTACAAATTTCCAAG
GTTGTGGTTGGGGTTGGGCTGTTTGTGCTTGGATTTGGTGTTTCCTTTGGTACATTCCAA
TGGATTTCATTAAATTAGGTGTCACCTATATTCTCCGTGGTAAGATTGAACCAATCAATA
AAGATGCACTCCGTAAAATCTATGGTTGGTTTGGTAAAGAAATCCCAAAGGAAGCAACTC
AAGTCTCTCATAAAGTTGCTGAACAACAAGCTAAAAGAGATGCTTTACATGCACAAGAAA
CCCGTCATAAATCTGTTGTTACCGATAATAAAGTTTAAAATAACAATAATATTTAAATAA
TGTATAAAATAGTTTTAAAATTTTAGCAACAAATAA
Length of connected seq. 1406
Full length Seq ID -
Full length Seq. -
Length of full length seq. -