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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP528
Library VH
(Link to library)
Clone ID VHP528
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12473-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHP528 (VHP528Q) /CSM/VH/VHP5-B/VHP528Q.Seq.d/
GTGTAATAACTTAATAAAATAAATGTTTTCTAGATTGTTAAATAGAGGAAACGGTGGTGT
AAATAAAAATATAACATCAGGACTATTATTAAGGAGAACAACAACAACAACAACAAGATT
ATCATATATTAATAATAGTCCTACCTTATCAATTAGATCATTTTGTTCAAAATCAACAAC
AATAACATCAGATGAAAAGTTAGATTTATCAGGTTATACAACAGATAGAATTAGAAATTT
TTCAATTATAGCGCATATTGATCATGGTAAAACAACATTAAGTACAAAGTTATTATCATT
GACAGGTACATTACCAAAGAGTATATATGGTAGTGGTGAAGATAGTTTGGTTCAAAAAGA
AAAGAGTGAAGAAAGAAGAGAACAATATCTTGATAAATTACAAGTCGAAAAAGAGAGAGG
TATAACAGTGAAGGCTCAAACATGTACAATGAAATATCGTAATAAAGAAGATGGCAAAGA
TTATCTATTGAATCTTATCGATACACCAGGTCATGTTGATTTTTCCTATGAAGTTAGTAG
AAGTTTAATGGCATGTCAAGGCGCGCTATTGGTGGTTGATGCAGTTCAAGGCGTGCAAGC
TCAAACAATGGCAAACTATTATTTAGCCTTGGATTCXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
cnnlik*MFSRLLNRGNGGVNKNITSGLLLRRTTTTTTRLSYINNSPTLSIRSFCSKSTT
ITSDEKLDLSGYTTDRIRNFSIIAHIDHGKTTLSTKLLSLTGTLPKSIYGSGEDSLVQKE
KSEERREQYLDKLQVEKERGITVKAQTCTMKYRNKEDGKDYLLNLIDTPGHVDFSYEVSR
SLMACQGALLVVDAVQGVQAQTMANYYLALD---
Translated Amino Acid sequence (All Frames) 
Frame A:
v**lnkinvf*ivk*rkrwck*kynirtiikennnnnnkiiiy***sylin*iilfkinn
nnir*kvrfirlynr*n*kffnysay*sw*nnikykviiidryitkeyiw*w*r*fgskr
ke*rkkrtis**itsrkrerynsegsnmyneis**rrwqrlsiesyrytrsc*ffl*s**
kfngmsrraigg*cssrrassnngkllfslg---


Frame B:
cnnlik*MFSRLLNRGNGGVNKNITSGLLLRRTTTTTTRLSYINNSPTLSIRSFCSKSTT
ITSDEKLDLSGYTTDRIRNFSIIAHIDHGKTTLSTKLLSLTGTLPKSIYGSGEDSLVQKE
KSEERREQYLDKLQVEKERGITVKAQTCTMKYRNKEDGKDYLLNLIDTPGHVDFSYEVSR
SLMACQGALLVVDAVQGVQAQTMANYYLALD---


Frame C:
vit**nkcfldc*ieetvv*iki*hqdyy*geqqqqqqdyhiliivlpyqldhfvqnqqq
*hqmks*iyqviqqieleifql*rilimvkqh*vqsyyh*qvhyqrvymvvvkivwfkkk
rvkkeennilinykskkrev*q*rlkhvq*nivikkmakiiy*ilsihqvmlifpmklve
v*whvkarywwlmqfkacklkqwqtii*pwi---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP528 (VHP528Q) /CSM/VH/VHP5-B/VHP528Q.Seq.d/                       1031   0.0
VHG757 (VHG757Q) /CSM/VH/VHG7-C/VHG757Q.Seq.d/                       1023   0.0
VHJ639 (VHJ639Q) /CSM/VH/VHJ6-B/VHJ639Q.Seq.d/                        882   0.0
VHQ370 (VHQ370Q) /CSM/VH/VHQ3-C/VHQ370Q.Seq.d/                        880   0.0
VHM371 (VHM371Q) /CSM/VH/VHM3-C/VHM371Q.Seq.d/                        702   0.0
VHO117 (VHO117Q) /CSM/VH/VHO1-A/VHO117Q.Seq.d/                         50   4e-05
VHL675 (VHL675Q) /CSM/VH/VHL6-D/VHL675Q.Seq.d/                         50   4e-05
VHL649 (VHL649Q) /CSM/VH/VHL6-C/VHL649Q.Seq.d/                         50   4e-05
VHJ186 (VHJ186Q) /CSM/VH/VHJ1-D/VHJ186Q.Seq.d/                         50   4e-05
VHE757 (VHE757Q) /CSM/VH/VHE7-C/VHE757Q.Seq.d/                         50   4e-05
own update 2003. 1. 7
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427403) Dictyostelium discoideum cDNA clone:ddv62g07, 5' ...  1031   0.0    2
(BJ421997) Dictyostelium discoideum cDNA clone:ddv44b15, 5' ...  1023   0.0    2
(BJ423788) Dictyostelium discoideum cDNA clone:ddv50m10, 5' ...   882   0.0    2
(BJ427804) Dictyostelium discoideum cDNA clone:ddv63l17, 5' ...   880   0.0    2
(BJ425432) Dictyostelium discoideum cDNA clone:ddv55n17, 5' ...   702   0.0    2
(EJ018189) 1095439022696 Global-Ocean-Sampling_GS-26-01-01-1...    76   6e-20  2
(EJ767840) 1092963327384 Global-Ocean-Sampling_GS-30-02-01-1...    72   4e-17  2
(EJ699068) 1092956029516 Global-Ocean-Sampling_GS-30-02-01-1...    58   1e-14  3
(EJ715416) 1092959288588 Global-Ocean-Sampling_GS-30-02-01-1...    60   1e-14  2
(EJ727631) 1092959676381 Global-Ocean-Sampling_GS-30-02-01-1...    60   1e-14  2
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP003487_12(AP003487|pid:none) Oryza sativa Japonica Group genom...   150   2e-35
AJ719687_1(AJ719687|pid:none) Gallus gallus mRNA for hypothetica...   148   2e-34
(Q8N442) RecName: Full=GTP-binding protein GUF1 homolog;  &BC036...   145   1e-33
CP001124_1864(CP001124|pid:none) Geobacter bemidjiensis Bem, com...   145   1e-33
(Q39US7) RecName: Full=GTP-binding protein lepA;                      144   2e-33
(P74751) RecName: Full=GTP-binding protein lepA;  &BA000022_3161...   144   2e-33
AK053420_1(AK053420|pid:none) Mus musculus 0 day neonate eyeball...   143   4e-33
BC057962_1(BC057962|pid:none) Mus musculus GUF1 GTPase homolog (...   143   4e-33
BC147985_1(BC147985|pid:none) Bos taurus GUF1 GTPase homolog (S....   143   5e-33
CP001298_1313(CP001298|pid:none) Methylobacterium chloromethanic...   142   6e-33
protein update 2009. 7.21
PSORT 

 psg: 0.57  gvh: 0.47  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.79  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 92.0 %: mitochondrial
	  8.0 %: nuclear

>> prediction for VHP528 is mit
5' end seq. ID VHP528F
5' end seq. 
>VHP528F.Seq
GTGTAATAACTTAATAAAATAAATGTTTTCTAGATTGTTAAATAGAGGAAACGGTGGTGT
AAATAAAAATATAACATCAGGACTATTATTAAGGAGAACAACAACAACAACAACAAGATT
ATCATATATTAATAATAGTCCTACCTTATCAATTAGATCATTTTGTTCAAAATCAACAAC
AATAACATCAGATGAAAAGTTAGATTTATCAGGTTATACAACAGATAGAATTAGAAATTT
TTCAATTATAGCGCATATTGATCATGGTAAAACAACATTAAGTACAAAGTTATTATCATT
GACAGGTACATTACCAAAGAGTATATATGGTAGTGGTGAAGATAGTTTGGTTCAAAAAGA
AAAGAGTGAAGAAAGAAGAGAACAATATCTTGATAAATTACAAGTCGAAAAAGAGAGAGG
TATAACAGTGAAGGCTCAAACATGTACAATGAAATATCGTAATAAAGAAGATGGCAAAGA
TTATCTATTGAATCTTATCGATACACCAGGTCATGTTGATTTTTCCTATGAAGTTAGTAG
AAGTTTAATGGCATGTCAAGGCGCGCTATTGGTGGTTGATGCAGTTCAAGGCGTGCAAGC
TCAAACAATGGCAAACTATTATTTAGCCTTGGATTCNNNNNNNNNN
Length of 5' end seq. 646
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -