NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP521
Library VH
(Link to library)
Clone ID VHP521
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13887-1
Original site URL
Representative seq. ID VHP521P
(Link to Original site)
Representative DNA sequence 
>VHP521 (VHP521Q) /CSM/VH/VHP5-A/VHP521Q.Seq.d/
AAAACAAAAAATGTCATTTGTAGTAGGTTTTGATTTCGGAACAAAGAATTGCACTATTGC
AGTTGCCCAAAAGGGTGGTGTAGATGTTATTGCCAACGAAGTTTCAAATCGTTTAACTCC
ATCAATGGTTTCATTTGGTGAAAAAGAGAGATACTTAGGTGAATCTGCTTTAACTAATCA
ATTAAGAAATATTAGAAATACAATTACAAATATTAAAAGATTTATTGGACAAGAATTTAA
AACAGATACAGTTCAAGAAGAATTAAAACATGAAATGTTTCAATCATACGAAATGGATAA
TGGATTTGTTGGATATAATGTAACATATGCAGGTGAACAATGTTCATTCTCAAGTGAAGC
AATTTTGGGTATGTTATTTGGTAAATTAAAAAAGACCACAGAAGCATTTGTAAACAATCC
AGTTCGTGATGTGGTAATCTCAGTACCAGTATTTTGGAATGATTATCAACGTCGTGCCAT
TTTAAATGCAGGTTCCATCGCAGGTTTAAATATCATTCGTTTAATCAATGAAACCACAGC
AACAGCACTTTCATATGGTATCTACAAAGAATGGTCAGAAACTGACCCAACCAACGTTTT
ATTCGTTGATGTTGGTGATTCAGCCACTTCTGTCTCTGCCGTTCAATACAAAAAAGGTCA
ACTCAAAXXXXXXXXXXCAAGAAAAGGTTTAACCAATGAACTCTTAAAGGCTGCCATCGA
AGAGGAAGGTCGTATGCAAGCTTCCGATCTTTTAGCCATTGAAACCGCCGAAAAGAAGAA
CGCCCTCGAAAGTTACATCTATGATATGCGTTCAAAATTACAATCATCCCTCAAAGAATA
CGTCACCCCAGCCGATGCTGAAACCTTTATGACTCAATTAAACAAACAAATGGATTGGTT
AGAAAGTGAAGAAGGTGAAGATCAAACTAAATCTGTTTACGCTGGTAAACTCGAAGAACT
CCGTCGTCTTGGTAATCCAATTCAAAAACGTAAACAAGACTTGGAAGATTATCCAGATGC
TGTCCAAACCCTTAAAAACATTGCTTCATACGTTAAAAACGAAGCTATGATTCCAGGTGA
AAGATATGACCACATTGAAAAGGAAGAAAAAGAAAAACTTTGCAAAGATTGTGATGATGC
CGTCGAATGGATTGATGCTTTAGTTGCCAAACAACAAGCAACTCCAAAGACTCAACCATG
TATTATCAATACCACTGAAGTACTCGCCAAAAAGACTCAATTAGAAGTTACCGCTAAAAT
GATCCTTGGTAAACCAAAACCAAAGCCAGTTGAAGTACCAAAGGAAGAACCAAAAGATAC
TCCAAGGAATCAAAAGACGCACCAGCTGAAGAACCAGTTGCAACTAAAGNATCAAAAGNA
GGANATATTTTAG
sequence update 2002.10.25
Translated Amino Acid sequence 
KQKMSFVVGFDFGTKNCTIAVAQKGGVDVIANEVSNRLTPSMVSFGEKERYLGESALTNQ
LRNIRNTITNIKRFIGQEFKTDTVQEELKHEMFQSYEMDNGFVGYNVTYAGEQCSFSSEA
ILGMLFGKLKKTTEAFVNNPVRDVVISVPVFWNDYQRRAILNAGSIAGLNIIRLINETTA
TALSYGIYKEWSETDPTNVLFVDVGDSATSVSAVQYKKGQLK---

---RKGLTNELLKAAIEEEGRMQASDLLAIETAEKKNALESYIYDMRSKLQSSLKEYVTP
ADAETFMTQLNKQMDWLESEEGEDQTKSVYAGKLEELRRLGNPIQKRKQDLEDYPDAVQT
LKNIASYVKNEAMIPGERYDHIEKEEKEKLCKDCDDAVEWIDALVAKQQATPKTQPCIIN
TTEVLAKKTQLEVTAKMILGKPKPKPVEVPKEEPKDTPRNQKTHQLKNQLQLKXQKXXIF
*
Translated Amino Acid sequence (All Frames) 
Frame A:
ktknvicsrf*frnkelhycscpkgwcrcycqrsfksfnsingfiw*kreilr*icfn*s
ikky*kynyky*kiywtri*nryssrrikt*nvsiirng*wicwi*cnicr*tmfilk*s
nfgyviw*ikkdhrsickqsss*cgnlstsile*lstschfkcrfhrrfkyhsfnq*nhs
nstfiwylqrmvrn*pnqrfir*cw*fshfclcrsiqkrstq---

---qekv*pmns*rlpskrkvvcklpif*plkppkrrtpskvtsmicvqnynhpskntsp
qpmlkpl*ln*tnkwig*kvkkvkiklnlftlvnsknsvvlviqfknvnktwkiiqmlsk
plktllhtlktkl*fqvkdmttlkrkkkknfakivmmpsnglml*lpnnkqlqrlnhvls
iplkyspkrln*klplk*slvnqnqsqlkyqrknqkilqgikrrts*rtscn*xikxrxy
f

Frame B:
KQKMSFVVGFDFGTKNCTIAVAQKGGVDVIANEVSNRLTPSMVSFGEKERYLGESALTNQ
LRNIRNTITNIKRFIGQEFKTDTVQEELKHEMFQSYEMDNGFVGYNVTYAGEQCSFSSEA
ILGMLFGKLKKTTEAFVNNPVRDVVISVPVFWNDYQRRAILNAGSIAGLNIIRLINETTA
TALSYGIYKEWSETDPTNVLFVDVGDSATSVSAVQYKKGQLK---

---kkrfnq*tlkgchrrgrsyasfrsfsh*nrrkeerprklhl*yafkitiipqrirhp
src*nlydsikqtnglvrk*rr*rsn*iclrw*trrtpssw*snskt*trlgrlsrccpn
p*khcfir*krsydsr*ki*ph*kgrkrktlqrl**crrmd*cfscqttsnskdstmyyq
yh*strqkdsirsyr*ndpw*tktkas*stkgrtkryskeskdapaeepvatkxskxgxi
l

Frame C:
nkkchl**vliseqriallqlpkrvv*mllptkfqiv*lhqwfhlvkkrdt*vnll*lin
*eileiqlqilkdlldknlkqiqfkkn*nmkcfnhtkwimdlldim*hmqvnnvhsqvkq
fwvcylvn*krpqkhl*tiqfvmw*sqyqyfgmiinvvpf*mqvpsqv*isfv*smkpqq
qhfhmvstkngqkltqptfyslmlviqpllslpfntkkvns---

---RKGLTNELLKAAIEEEGRMQASDLLAIETAEKKNALESYIYDMRSKLQSSLKEYVTP
ADAETFMTQLNKQMDWLESEEGEDQTKSVYAGKLEELRRLGNPIQKRKQDLEDYPDAVQT
LKNIASYVKNEAMIPGERYDHIEKEEKEKLCKDCDDAVEWIDALVAKQQATPKTQPCIIN
TTEVLAKKTQLEVTAKMILGKPKPKPVEVPKEEPKDTPRNQKTHQLKNQLQLKXQKXXIF
*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP521 (VHP521Q) /CSM/VH/VHP5-A/VHP521Q.Seq.d/                       2113   0.0
SLA563 (SLA563Q) /CSM/SL/SLA5-C/SLA563Q.Seq.d/                       1320   0.0
CHS482 (CHS482Q) /CSM/CH/CHS4-D/CHS482Q.Seq.d/                       1304   0.0
VHI779 (VHI779Q) /CSM/VH/VHI7-D/VHI779Q.Seq.d/                       1296   0.0
VHL621 (VHL621Q) /CSM/VH/VHL6-A/VHL621Q.Seq.d/                       1291   0.0
AHQ273 (AHQ273Q) /CSM/AH/AHQ2-D/AHQ273Q.Seq.d/                       1271   0.0
VHG630 (VHG630Q) /CSM/VH/VHG6-B/VHG630Q.Seq.d/                       1263   0.0
VHG245 (VHG245Q) /CSM/VH/VHG2-B/VHG245Q.Seq.d/                       1263   0.0
CHQ816 (CHQ816Q) /CSM/CH/CHQ8-A/CHQ816Q.Seq.d/                       1263   0.0
AHL809 (AHL809Q) /CSM/AH/AHL8-A/AHL809Q.Seq.d/                       1263   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427343) Dictyostelium discoideum cDNA clone:ddv62i05, 5' ...  1322   0.0    1
(AU060200) Dictyostelium discoideum slug cDNA, clone SLA563.     1320   0.0    1
(BJ371620) Dictyostelium discoideum cDNA clone:ddc58d22, 5' ...  1304   0.0    1
(BJ423359) Dictyostelium discoideum cDNA clone:ddv48n19, 5' ...  1296   0.0    1
(BJ424941) Dictyostelium discoideum cDNA clone:ddv54i06, 5' ...  1291   0.0    1
(BJ338987) Dictyostelium discoideum cDNA clone:dda63a20, 5' ...  1271   0.0    1
(BJ421959) Dictyostelium discoideum cDNA clone:ddv44k08, 5' ...  1263   0.0    1
(BJ421643) Dictyostelium discoideum cDNA clone:ddv43i12, 5' ...  1263   0.0    1
(BJ370742) Dictyostelium discoideum cDNA clone:ddc55p04, 5' ...  1263   0.0    1
(BJ336464) Dictyostelium discoideum cDNA clone:dda54b04, 5' ...  1263   0.0    1
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC009322_1(AC009322|pid:none) Arabidopsis thaliana chromosome I ...   195   3e-48
(Q5R606) RecName: Full=Heat shock protein 105 kDa; AltName: Full...   195   4e-48
D86956_1(D86956|pid:none) Homo sapiens mRNA for KIAA0201 gene, p...   195   4e-48
AF039695_1(AF039695|pid:none) Homo sapiens antigen NY-CO-25 (NY-...   195   4e-48
CU638743_280(CU638743|pid:none) Podospora anserina genomic DNA c...   194   6e-48
(Q61316) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   194   6e-48
(Q0IIM3) RecName: Full=Heat shock protein 105 kDa; AltName: Full...   193   1e-47
AB023420_1(AB023420|pid:none) Homo sapiens mRNA for heat shock p...   193   1e-47
X67643_1(X67643|pid:none) H.sapiens HS24/p52 mRNA.                    193   1e-47
(O88600) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   192   2e-47
protein update 2009. 7.21
PSORT 

 psg: 0.74  gvh: 0.36  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHP521 is cyt
5' end seq. ID VHP521F
5' end seq. 
>VHP521F.Seq
AAAACAAAAAATGTCATTTGTAGTAGGTTTTGATTTCGGAACAAAGAATTGCACTATTGC
AGTTGCCCAAAAGGGTGGTGTAGATGTTATTGCCAACGAAGTTTCAAATCGTTTAACTCC
ATCAATGGTTTCATTTGGTGAAAAAGAGAGATACTTAGGTGAATCTGCTTTAACTAATCA
ATTAAGAAATATTAGAAATACAATTACAAATATTAAAAGATTTATTGGACAAGAATTTAA
AACAGATACAGTTCAAGAAGAATTAAAACATGAAATGTTTCAATCATACGAAATGGATAA
TGGATTTGTTGGATATAATGTAACATATGCAGGTGAACAATGTTCATTCTCAAGTGAAGC
AATTTTGGGTATGTTATTTGGTAAATTAAAAAAGACCACAGAAGCATTTGTAAACAATCC
AGTTCGTGATGTGGTAATCTCAGTACCAGTATTTTGGAATGATTATCAACGTCGTGCCAT
TTTAAATGCAGGTTCCATCGCAGGTTTAAATATCATTCGTTTAATCAATGAAACCACAGC
AACAGCACTTTCATATGGTATCTACAAAGAATGGTCAGAAACTGACCCAACCAACGTTTT
ATTCGTTGATGTTGGTGATTCAGCCACTTCTGTCTCTGCCGTTCAATACAAAAAAGGTCA
ACTCAAANNNNNNNNNN
Length of 5' end seq. 677
3' end seq. ID VHP521Z
3' end seq. 
>VHP521Z.Seq
NNNNNNNNNNCAAGAAAAGGTTTAACCAATGAACTCTTAAAGGCTGCCATCGAAGAGGAA
GGTCGTATGCAAGCTTCCGATCTTTTAGCCATTGAAACCGCCGAAAAGAAGAACGCCCTC
GAAAGTTACATCTATGATATGCGTTCAAAATTACAATCATCCCTCAAAGAATACGTCACC
CCAGCCGATGCTGAAACCTTTATGACTCAATTAAACAAACAAATGGATTGGTTAGAAAGT
GAAGAAGGTGAAGATCAAACTAAATCTGTTTACGCTGGTAAACTCGAAGAACTCCGTCGT
CTTGGTAATCCAATTCAAAAACGTAAACAAGACTTGGAAGATTATCCAGATGCTGTCCAA
ACCCTTAAAAACATTGCTTCATACGTTAAAAACGAAGCTATGATTCCAGGTGAAAGATAT
GACCACATTGAAAAGGAAGAAAAAGAAAAACTTTGCAAAGATTGTGATGATGCCGTCGAA
TGGATTGATGCTTTAGTTGCCAAACAACAAGCAACTCCAAAGACTCAACCATGTATTATC
AATACCACTGAAGTACTCGCCAAAAAGACTCAATTAGAAGTTACCGCTAAAATGATCCTT
GGTAAACCAAAACCAAAGCCAGTTGAAGTACCAAAGGAAGAACCAAAAGATACTCCAAGG
AATCAAAAGACGCACCAGCTGAAGAACCAGTTGCAACTAAAGNATCAAAAGNAGGANATA
TTTTAG
Length of 3' end seq. 726
Connected seq. ID VHP521P
Connected seq. 
>VHP521P.Seq
AAAACAAAAAATGTCATTTGTAGTAGGTTTTGATTTCGGAACAAAGAATTGCACTATTGC
AGTTGCCCAAAAGGGTGGTGTAGATGTTATTGCCAACGAAGTTTCAAATCGTTTAACTCC
ATCAATGGTTTCATTTGGTGAAAAAGAGAGATACTTAGGTGAATCTGCTTTAACTAATCA
ATTAAGAAATATTAGAAATACAATTACAAATATTAAAAGATTTATTGGACAAGAATTTAA
AACAGATACAGTTCAAGAAGAATTAAAACATGAAATGTTTCAATCATACGAAATGGATAA
TGGATTTGTTGGATATAATGTAACATATGCAGGTGAACAATGTTCATTCTCAAGTGAAGC
AATTTTGGGTATGTTATTTGGTAAATTAAAAAAGACCACAGAAGCATTTGTAAACAATCC
AGTTCGTGATGTGGTAATCTCAGTACCAGTATTTTGGAATGATTATCAACGTCGTGCCAT
TTTAAATGCAGGTTCCATCGCAGGTTTAAATATCATTCGTTTAATCAATGAAACCACAGC
AACAGCACTTTCATATGGTATCTACAAAGAATGGTCAGAAACTGACCCAACCAACGTTTT
ATTCGTTGATGTTGGTGATTCAGCCACTTCTGTCTCTGCCGTTCAATACAAAAAAGGTCA
ACTCAAA----------CAAGAAAAGGTTTAACCAATGAACTCTTAAAGGCTGCCATCGA
AGAGGAAGGTCGTATGCAAGCTTCCGATCTTTTAGCCATTGAAACCGCCGAAAAGAAGAA
CGCCCTCGAAAGTTACATCTATGATATGCGTTCAAAATTACAATCATCCCTCAAAGAATA
CGTCACCCCAGCCGATGCTGAAACCTTTATGACTCAATTAAACAAACAAATGGATTGGTT
AGAAAGTGAAGAAGGTGAAGATCAAACTAAATCTGTTTACGCTGGTAAACTCGAAGAACT
CCGTCGTCTTGGTAATCCAATTCAAAAACGTAAACAAGACTTGGAAGATTATCCAGATGC
TGTCCAAACCCTTAAAAACATTGCTTCATACGTTAAAAACGAAGCTATGATTCCAGGTGA
AAGATATGACCACATTGAAAAGGAAGAAAAAGAAAAACTTTGCAAAGATTGTGATGATGC
CGTCGAATGGATTGATGCTTTAGTTGCCAAACAACAAGCAACTCCAAAGACTCAACCATG
TATTATCAATACCACTGAAGTACTCGCCAAAAAGACTCAATTAGAAGTTACCGCTAAAAT
GATCCTTGGTAAACCAAAACCAAAGCCAGTTGAAGTACCAAAGGAAGAACCAAAAGATAC
TCCAAGGAATCAAAAGACGCACCAGCTGAAGAACCAGTTGCAACTAAAGNATCAAAAGNA
GGANATATTTTAG
Length of connected seq. 1383
Full length Seq ID -
Full length Seq. -
Length of full length seq. -