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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP494
Library VH
(Link to library)
Clone ID VHP494
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16433-1
Original site URL
Representative seq. ID VHP494P
(Link to Original site)
Representative DNA sequence 
>VHP494 (VHP494Q) /CSM/VH/VHP4-D/VHP494Q.Seq.d/
GTGCTGAAGATATCGTTGATAGTCAATTGAAATTAATTCTTGGTTTAATTTGGACCTTGA
TTTTACGTTATCAAATTCAAATGTCTGAAAGTGATAACTCACCAAAAGCAGCTCTCTTGG
AATGGGTTAGAAAGCAAGTTGCCCCATACAAGGTTGTTGTAAACAATTTCACAGACTCAT
GGTGTGATGGTCGTGTTCTCTCTGCTCTCACTGATTCCCTCAAACCAGGTGTTAGAGAGA
TGTCAACCCTCACCGGTGATGCCGTTCAAGATATCGACAGATCCATGGATATCGCTCTTG
AAGAATATGAAATCCCAAAGATTATGGATGCCAATGATATGAACTCTCTTCCAGATGAAT
TATCAGTCATCACCTACGTCTCTTATTTCCGTGATTACGCACTCAACAAAGAAAAGAGAG
ATGCTGATGCTTTAGCTGCCCTCGAAAAGAAACGTCGTGAAACTTCAGATGCCAGCAAGG
TTGAAGTTTATGGTCCAGGTGTTGAAGGTGGTTTCGTTAATAAATCCGCXXXXXXXXXXC
CAGCTCCAGTCGATCCAGTTATGGTCGATAATGGTGATGGTACATATGATGTTGAATTTG
AACCAAAAGAAGCCGGTGACTATGTTATCAATCTCACTTTAGATGGTGACAACGTCAATG
GTTTCCCAAAGACTGTTACCGTTAAACCAGCCCCATCCGCTGAACACTCTTATGCTGAAG
GTGAAGGTTTAGTCAAAGTATTTGATAATGCCCCAGCTGAATTCACTATTTTCGCCGTTG
ACACTAAAGGTGTTGCTCGTACCGATGGTGATGATCCATTTGAAGTCGCTATCAATGGTC
CAGATGGTTTAGTCGTTGATGCCAAAGTTACCGATAACAATGACGGTACTTATGGTGTTG
TCTATGATGCCCCAGTTGAAGGTAACTACAATGTTAATGTCACCCTCCGTGGTAATCCAA
TCAAAAATATGCCAATCGATGTCAAATGCATTGAAGGTGCCAATGGTGAAGATTCATCAT
TCGGTTCATTCACTTTTACCGTCGCTGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATG
GTGGTGATAAATTTGAAGTCTCTATCACTGGTCCAGCTGAAGAAATCACTCTCGATGCTA
TTGATAACCAAGATGGTACTTATACTGCCGCTTACTCTTTAGTTGGTAATGGTCGTTTCT
CAACTGGTGTCAAATTAAACGGTAAACACATTGAAGGTTCTCCATTCAAACAAGTACTTG
GTAGCCCAGGTAAAAAGAATCCAGAAGTTAAATCTTTCACTACTACTCGTACTGCCAATT
AAAAAAAAGCATTAAGTAATAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
AEDIVDSQLKLILGLIWTLILRYQIQMSESDNSPKAALLEWVRKQVAPYKVVVNNFTDSW
CDGRVLSALTDSLKPGVREMSTLTGDAVQDIDRSMDIALEEYEIPKIMDANDMNSLPDEL
SVITYVSYFRDYALNKEKRDADALAALEKKRRETSDASKVEVYGPGVEGGFVNKS---

---PAPVDPVMVDNGDGTYDVEFEPKEAGDYVINLTLDGDNVNGFPKTVTVKPAPSAEHS
YAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGDDPFEVAINGPDGLVVDAKVTDNNDGT
YGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEGANGEDSSFGSFTFTVAAKNKKGEV
KTYGGDKFEVSITGPAEEITLDAIDNQDGTYTAAYSLVGNGRFSTGVKLNGKHIEGSPFK
QVLGSPGKKNPEVKSFTTTRTAN*kkalsnn
Translated Amino Acid sequence (All Frames) 
Frame A:
vlkislivn*n*flv*fgp*fyvikfkclkvithqkqlswnglesklphtrll*tisqth
gvmvvfsllslipsnqvlercqpspvmpfkistdpwisllknmksqrlwmpmi*tlfqmn
yqssptslisvithstkkremlml*lpskrnvvklqmparlkfmvqvlkvvslinp---

---PAPVDPVMVDNGDGTYDVEFEPKEAGDYVINLTLDGDNVNGFPKTVTVKPAPSAEHS
YAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGDDPFEVAINGPDGLVVDAKVTDNNDGT
YGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEGANGEDSSFGSFTFTVAAKNKKGEV
KTYGGDKFEVSITGPAEEITLDAIDNQDGTYTAAYSLVGNGRFSTGVKLNGKHIEGSPFK
QVLGSPGKKNPEVKSFTTTRTAN*kkalsnn

Frame B:
c*ryr**sieinswfnldldftlsnsnv*k**ltkssslgmg*kascpiqgcckqfhrlm
v*wscslcsh*fpqtrc*rdvnphr*crsryrqihgyrs*ri*npkdygcq*yelssr*i
ishhlrllfp*lrtqqrkerc*cfscprkets*nfrcqqg*slwsrc*rwfr**ir---

---qlqsiqlwsimvmvhmmlnlnqkkpvtmlsisl*mvttsmvsqrllplnqphplntl
mlkvkv*skylimpqlnslfspltlkvllvpmvmihlkslsmvqmv*slmpklpitmtvl
mvlsmmpqlkvttmlmspsvviqskicqsmsnalkvpmvkihhsvhsllpsllkirkvkl
kpmvvinlkslslvqlkkslsmllitkmvlilpltl*lvmvvsqlvsn*tvntlkvlhsn
kylvaqvkriqklnlslllvlpikkkh*vii

Frame C:
AEDIVDSQLKLILGLIWTLILRYQIQMSESDNSPKAALLEWVRKQVAPYKVVVNNFTDSW
CDGRVLSALTDSLKPGVREMSTLTGDAVQDIDRSMDIALEEYEIPKIMDANDMNSLPDEL
SVITYVSYFRDYALNKEKRDADALAALEKKRRETSDASKVEVYGPGVEGGFVNKS---

---sssrssygr*w*wyi*c*i*tkrsr*lcyqshfrw*qrqwfpkdcyr*tspir*tll
c*r*rfsqsi**cps*ihyfrr*h*rccsyrw**si*sryqwsrwfsr*cqsyr*q*ryl
wccl*cps*r*lqc*chppw*snqkyanrcqmh*rcqw*rfiirfihfyrrc*k*er*s*
nlww**i*slyhwss*rnhsrcy**prwylycrllfsw*wsflnwcqikr*th*rfsiqt
stw*pr*kesrs*ifhyysycqlkksik***
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP494 (VHP494Q) /CSM/VH/VHP4-D/VHP494Q.Seq.d/                       2559   0.0
SFD611 (SFD611Q) /CSM/SF/SFD6-A/SFD611Q.Seq.d/                       1516   0.0
AHF883 (AHF883Q) /CSM/AH/AHF8-D/AHF883Q.Seq.d/                       1509   0.0
AHD645 (AHD645Q) /CSM/AH/AHD6-B/AHD645Q.Seq.d/                       1477   0.0
AFN847 (AFN847Q) /CSM/AF/AFN8-B/AFN847Q.Seq.d/                       1449   0.0
SFH146 (SFH146Q) /CSM/SF/SFH1-B/SFH146Q.Seq.d/                       1447   0.0
CHQ595 (CHQ595Q) /CSM/CH/CHQ5-D/CHQ595Q.Seq.d/                       1435   0.0
VHP348 (VHP348Q) /CSM/VH/VHP3-B/VHP348Q.Seq.d/                       1425   0.0
AFN630 (AFN630Q) /CSM/AF/AFN6-B/AFN630Q.Seq.d/                       1419   0.0
VFK681 (VFK681Q) /CSM/VF/VFK6-D/VFK681Q.Seq.d/                       1398   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446180) Dictyostelium discoideum cDNA clone:ddv61l24, 3' ...  1548   0.0    2
(X15430) Dictyostelium discoideum mRNA for gelation factor.      1532   0.0    2
(BJ351325) Dictyostelium discoideum cDNA clone:dda42f22, 3' ...  1509   0.0    2
(BJ350003) Dictyostelium discoideum cDNA clone:dda38i12, 3' ...  1477   0.0    2
(BJ345672) Dictyostelium discoideum cDNA clone:dda28n12, 3' ...  1449   0.0    2
(BJ400915) Dictyostelium discoideum cDNA clone:dds15k11, 3' ...  1447   0.0    1
(BJ385719) Dictyostelium discoideum cDNA clone:ddc55m23, 3' ...  1435   0.0    1
(BJ446045) Dictyostelium discoideum cDNA clone:ddv61p11, 3' ...  1425   0.0    1
(BJ345658) Dictyostelium discoideum cDNA clone:dda28k08, 3' ...  1417   0.0    1
(BJ433717) Dictyostelium discoideum cDNA clone:ddv22a22, 3' ...  1398   0.0    1
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70560_1(U70560|pid:none) Entamoeba histolytica actin binding pr...   197   9e-49
AY382663_1(AY382663|pid:none) Hirudo medicinalis filamin mRNA, c...   129   2e-28
AE014297_2391(AE014297|pid:none) Drosophila melanogaster chromos...   120   9e-26
AF174492_1(AF174492|pid:none) Drosophila melanogaster filamin1 m...   120   9e-26
AB056474_1(AB056474|pid:none) Gallus gallus mRNA for filamin, co...   119   2e-25
BC151024_1(BC151024|pid:none) Mus musculus filamin, alpha, mRNA ...   119   3e-25
AL807376_2(AL807376|pid:none) Mouse DNA sequence from clone RP23...   119   3e-25
AK089311_1(AK089311|pid:none) Mus musculus B6-derived CD11 +ve d...   119   3e-25
AL807376_1(AL807376|pid:none) Mouse DNA sequence from clone RP23...   119   3e-25
AK152579_1(AK152579|pid:none) Mus musculus bone marrow macrophag...   119   3e-25
protein update 2009. 7.21
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for VHP494 is cyt
5' end seq. ID VHP494F
5' end seq. 
>VHP494F.Seq
GTGCTGAAGATATCGTTGATAGTCAATTGAAATTAATTCTTGGTTTAATTTGGACCTTGA
TTTTACGTTATCAAATTCAAATGTCTGAAAGTGATAACTCACCAAAAGCAGCTCTCTTGG
AATGGGTTAGAAAGCAAGTTGCCCCATACAAGGTTGTTGTAAACAATTTCACAGACTCAT
GGTGTGATGGTCGTGTTCTCTCTGCTCTCACTGATTCCCTCAAACCAGGTGTTAGAGAGA
TGTCAACCCTCACCGGTGATGCCGTTCAAGATATCGACAGATCCATGGATATCGCTCTTG
AAGAATATGAAATCCCAAAGATTATGGATGCCAATGATATGAACTCTCTTCCAGATGAAT
TATCAGTCATCACCTACGTCTCTTATTTCCGTGATTACGCACTCAACAAAGAAAAGAGAG
ATGCTGATGCTTTAGCTGCCCTCGAAAAGAAACGTCGTGAAACTTCAGATGCCAGCAAGG
TTGAAGTTTATGGTCCAGGTGTTGAAGGTGGTTTCGTTAATAAATCCGCNNNNNNNNNN
Length of 5' end seq. 539
3' end seq. ID VHP494Z
3' end seq. 
>VHP494Z.Seq
NNNNNNNNNNCCAGCTCCAGTCGATCCAGTTATGGTCGATAATGGTGATGGTACATATGA
TGTTGAATTTGAACCAAAAGAAGCCGGTGACTATGTTATCAATCTCACTTTAGATGGTGA
CAACGTCAATGGTTTCCCAAAGACTGTTACCGTTAAACCAGCCCCATCCGCTGAACACTC
TTATGCTGAAGGTGAAGGTTTAGTCAAAGTATTTGATAATGCCCCAGCTGAATTCACTAT
TTTCGCCGTTGACACTAAAGGTGTTGCTCGTACCGATGGTGATGATCCATTTGAAGTCGC
TATCAATGGTCCAGATGGTTTAGTCGTTGATGCCAAAGTTACCGATAACAATGACGGTAC
TTATGGTGTTGTCTATGATGCCCCAGTTGAAGGTAACTACAATGTTAATGTCACCCTCCG
TGGTAATCCAATCAAAAATATGCCAATCGATGTCAAATGCATTGAAGGTGCCAATGGTGA
AGATTCATCATTCGGTTCATTCACTTTTACCGTCGCTGCTAAAAATAAGAAAGGTGAAGT
TAAAACCTATGGTGGTGATAAATTTGAAGTCTCTATCACTGGTCCAGCTGAAGAAATCAC
TCTCGATGCTATTGATAACCAAGATGGTACTTATACTGCCGCTTACTCTTTAGTTGGTAA
TGGTCGTTTCTCAACTGGTGTCAAATTAAACGGTAAACACATTGAAGGTTCTCCATTCAA
ACAAGTACTTGGTAGCCCAGGTAAAAAGAATCCAGAAGTTAAATCTTTCACTACTACTCG
TACTGCCAATTAAAAAAAAGCATTAAGTAATAATAA
Length of 3' end seq. 816
Connected seq. ID VHP494P
Connected seq. 
>VHP494P.Seq
GTGCTGAAGATATCGTTGATAGTCAATTGAAATTAATTCTTGGTTTAATTTGGACCTTGA
TTTTACGTTATCAAATTCAAATGTCTGAAAGTGATAACTCACCAAAAGCAGCTCTCTTGG
AATGGGTTAGAAAGCAAGTTGCCCCATACAAGGTTGTTGTAAACAATTTCACAGACTCAT
GGTGTGATGGTCGTGTTCTCTCTGCTCTCACTGATTCCCTCAAACCAGGTGTTAGAGAGA
TGTCAACCCTCACCGGTGATGCCGTTCAAGATATCGACAGATCCATGGATATCGCTCTTG
AAGAATATGAAATCCCAAAGATTATGGATGCCAATGATATGAACTCTCTTCCAGATGAAT
TATCAGTCATCACCTACGTCTCTTATTTCCGTGATTACGCACTCAACAAAGAAAAGAGAG
ATGCTGATGCTTTAGCTGCCCTCGAAAAGAAACGTCGTGAAACTTCAGATGCCAGCAAGG
TTGAAGTTTATGGTCCAGGTGTTGAAGGTGGTTTCGTTAATAAATCCGC----------C
CAGCTCCAGTCGATCCAGTTATGGTCGATAATGGTGATGGTACATATGATGTTGAATTTG
AACCAAAAGAAGCCGGTGACTATGTTATCAATCTCACTTTAGATGGTGACAACGTCAATG
GTTTCCCAAAGACTGTTACCGTTAAACCAGCCCCATCCGCTGAACACTCTTATGCTGAAG
GTGAAGGTTTAGTCAAAGTATTTGATAATGCCCCAGCTGAATTCACTATTTTCGCCGTTG
ACACTAAAGGTGTTGCTCGTACCGATGGTGATGATCCATTTGAAGTCGCTATCAATGGTC
CAGATGGTTTAGTCGTTGATGCCAAAGTTACCGATAACAATGACGGTACTTATGGTGTTG
TCTATGATGCCCCAGTTGAAGGTAACTACAATGTTAATGTCACCCTCCGTGGTAATCCAA
TCAAAAATATGCCAATCGATGTCAAATGCATTGAAGGTGCCAATGGTGAAGATTCATCAT
TCGGTTCATTCACTTTTACCGTCGCTGCTAAAAATAAGAAAGGTGAAGTTAAAACCTATG
GTGGTGATAAATTTGAAGTCTCTATCACTGGTCCAGCTGAAGAAATCACTCTCGATGCTA
TTGATAACCAAGATGGTACTTATACTGCCGCTTACTCTTTAGTTGGTAATGGTCGTTTCT
CAACTGGTGTCAAATTAAACGGTAAACACATTGAAGGTTCTCCATTCAAACAAGTACTTG
GTAGCCCAGGTAAAAAGAATCCAGAAGTTAAATCTTTCACTACTACTCGTACTGCCAATT
AAAAAAAAGCATTAAGTAATAATAA
Length of connected seq. 1335
Full length Seq ID -
Full length Seq. -
Length of full length seq. -