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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP475
Library VH
(Link to library)
Clone ID VHP475
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16430-1
Original site URL
Representative seq. ID VHP475P
(Link to Original site)
Representative DNA sequence 
>VHP475 (VHP475Q) /CSM/VH/VHP4-D/VHP475Q.Seq.d/
GTTGGCCTACTGGTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAGAACA
TAAAAGAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCT
TATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGAT
TTCAAATGCATCTGATGCCTTAGGTAAAATTCGTTACCAATCATTAACTGATGCATCAGT
TTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCT
TACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTAC
TATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATAT
CAGTATGATTGGCCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGN
NATTGTTCACTCTXXXXXXXXXXTGAAGTCATTTACATGTGTGACCCAATTGATGAATAT
GCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGT
TTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAAT
GAAGAATTACTCAAACAAGTTAAAGATGTATTGGGCGATAAAGTTGAAAAGGTAGTTCTC
TCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCA
AACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATG
TCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAA
AAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCT
CTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGT
ATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACA
AACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGG
AAAAAGTCGACTAGATTAATTACTAGCCCTTTTGCTATCAATTTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
laywyilihn*FYLFFFVEHKRMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELI
SNASDALGKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGT
IARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTXIVHS---

---EVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELLK
QVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKKT
LELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKLG
LSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRLITSPFAINF
Translated Amino Acid sequence (All Frames) 
Frame A:
vgllvysns*lilfvffcrt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkrid
fkci*clr*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefry
ycsfry*klygtitkwcr*yqydwpiwcwfllcllscryxxcsl---

---*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*tr*scq*ritq
ts*rcigr*s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*lfnviihviken
friksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiiicftyssyd*ir
fkhsr*fisnn*rinkhnnq**ystirrkr*ti*kekvd*iny*pfcyqf

Frame B:
laywyilihn*FYLFFFVEHKRMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELI
SNASDALGKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGT
IARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTXIVHS---

---EVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELLK
QVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKKT
LELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKLG
LSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRLITSPFAINF

Frame C:
wptgif*fiinficffl*nikewlnhklnvshsklklislcplllilfiqikkyf*en*f
qmhlmp*vkfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*vl
llvqvlktlwnnykvvplisv*lanlvlvstllt*lpilxlft---

---ksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkklnkiklpmknysn
klkmywaiklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvtlqchhtchqrkl
*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhllhvfiv*ln*v
*afkmihqqqlknqqtqqpvmifhh*kktmnhlkgksrld*llalllsif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP475 (VHP475Q) /CSM/VH/VHP4-D/VHP475Q.Seq.d/                       1653   0.0
VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        862   0.0
VHP779 (VHP779Q) /CSM/VH/VHP7-D/VHP779Q.Seq.d/                        862   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                        862   0.0
VHP152 (VHP152Q) /CSM/VH/VHP1-C/VHP152Q.Seq.d/                        862   0.0
VHM808 (VHM808Q) /CSM/VH/VHM8-A/VHM808Q.Seq.d/                        862   0.0
VHM703 (VHM703Q) /CSM/VH/VHM7-A/VHM703Q.Seq.d/                        862   0.0
VHL676 (VHL676Q) /CSM/VH/VHL6-D/VHL676Q.Seq.d/                        862   0.0
VHL421 (VHL421Q) /CSM/VH/VHL4-A/VHL421Q.Seq.d/                        862   0.0
VHK487 (VHK487Q) /CSM/VH/VHK4-D/VHK487Q.Seq.d/                        862   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(L43591) Dictyostelium discoideum (clone C 6.1.1) heat shock...   839   0.0    4
(BJ446149) Dictyostelium discoideum cDNA clone:ddv61f20, 3' ...   781   0.0    2
(BJ446614) Dictyostelium discoideum cDNA clone:ddv63d12, 3' ...   773   0.0    3
(BJ446603) Dictyostelium discoideum cDNA clone:ddv63b09, 3' ...   773   0.0    4
(BJ446503) Dictyostelium discoideum cDNA clone:ddv62n19, 3' ...   773   0.0    4
(BJ446041) Dictyostelium discoideum cDNA clone:ddv61o11, 3' ...   773   0.0    4
(BJ445907) Dictyostelium discoideum cDNA clone:ddv61c02, 3' ...   773   0.0    4
(BJ445748) Dictyostelium discoideum cDNA clone:ddv60c16, 3' ...   773   0.0    4
(BJ445137) Dictyostelium discoideum cDNA clone:ddv58e17, 3' ...   773   0.0    4
(BJ445133) Dictyostelium discoideum cDNA clone:ddv58d18, 3' ...   773   0.0    3
dna update 2008.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             295   2e-78
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   295   2e-78
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   216   1e-54
AB092411_1(AB092411|pid:none) Entamoeba histolytica gene for cyt...   210   9e-53
EF687776_1(EF687776|pid:none) Crassostrea gigas heat shock prote...   209   2e-52
DQ062086_1(DQ062086|pid:none) Pseudourostyla cristata heat shock...   205   2e-51
U39048_1(U39048|pid:none) Achlya ambisexualis heat shock protein...   205   2e-51
U39047_1(U39047|pid:none) Achlya ambisexualis heat shock protein...   205   2e-51
AF402101_1(AF402101|pid:none) Achlya ambisexualis isolate JS855 ...   205   2e-51
AF402100_1(AF402100|pid:none) Achlya ambisexualis isolate JS854 ...   205   2e-51
protein update 2009. 7.21
PSORT 

 psg: 0.50  gvh: 0.46  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHP475 is nuc
5' end seq. ID VHP475F
5' end seq. 
>VHP475F.Seq
GTTGGCCTACTGGTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAGAACA
TAAAAGAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCT
TATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGAT
TTCAAATGCATCTGATGCCTTAGGTAAAATTCGTTACCAATCATTAACTGATGCATCAGT
TTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCT
TACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTAC
TATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATAT
CAGTATGATTGGCCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGN
NATTGTTCACTCTNNNNNNNNNN
Length of 5' end seq. 503
3' end seq. ID VHP475Z
3' end seq. 
>VHP475Z.Seq
NNNNNNNNNNTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAAT
TAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGATG
AAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCA
AACAAGTTAAAGATGTATTGGGCGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTG
CCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTA
TCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAA
CTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAA
AAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAG
GTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAG
GTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCA
GTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTCGACTA
GATTAATTACTAGCCCTTTTGCTATCAATTTTT
Length of 3' end seq. 693
Connected seq. ID VHP475P
Connected seq. 
>VHP475P.Seq
GTTGGCCTACTGGTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAGAACA
TAAAAGAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCT
TATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGAT
TTCAAATGCATCTGATGCCTTAGGTAAAATTCGTTACCAATCATTAACTGATGCATCAGT
TTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCT
TACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTAC
TATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATAT
CAGTATGATTGGCCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGN
NATTGTTCACTCT----------TGAAGTCATTTACATGTGTGACCCAATTGATGAATAT
GCTGTTCAACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGT
TTGAAATTAGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAAT
GAAGAATTACTCAAACAAGTTAAAGATGTATTGGGCGATAAAGTTGAAAAGGTAGTTCTC
TCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCA
AACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATG
TCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAA
AAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCT
CTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGT
ATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACA
AACACAACAACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGG
AAAAAGTCGACTAGATTAATTACTAGCCCTTTTGCTATCAATTTTT
Length of connected seq. 1176
Full length Seq ID -
Full length Seq. -
Length of full length seq. -