>AFO831 (AFO831Q) /CSM/AF/AFO8-B/AFO831Q.Seq.d/
XXXXXXXXXXTCTTCCAAAGATTACCNCCCATTCATACCTATCATATGAATCTTTCAAAA
TATCTCTCACCCGAATACGATCTTCAAGTAAAGAAACGTATTATGAGAGCCACCGAAGTT
GATACTTTAAATTCAATACCAATGATGTTCTCAACTTCAAAATAGATTCGTTTATTATGT
ACCAGCAAAATCAAATGACGATCCAAGATGGGATGCAGTTATCGATAAACTTCAAATGGT
TGGTAGAGGCAATGGTGGCGCTGTTAGAGGTATCTATAAAGGTGTTATTGAGACCACTTT
CTTTGGCAAAAGAGTTTATTTGGTTGGTACCTATTCACCATTCTATAGTCAACAACAAAG
AAATAAAAGTCAACGTATACTTTTCAATGATTTTCCAAAGGAAGAATTTGATCCAACCAT
CACAAATCCTTTAACAAGTACTATGCTATTTAGAACTGATAAAGATCTCTCATTGGATAT
TACTTTGGCTGAAAAAGTTTTCTCTTGTAAAGACTTTTGTGAAAAACATAAAGTATCATG
TTTAGAATCTGATATGATGTTATTAACTGATAGTACTATAGTTTTACTACAATTGGAACA
ACATTGTAGTCAAGTAAACTATATACCAAGTAATAATACCGATACATTTTATCCAATCAA
AGATAAAG

---skdyxpfipii*ifqnishpntifk*rnvl*eppklil*iqyq*CSQLQNRFVYYVP
AKSNDDPRWDAVIDKLQMVGRGNGGAVRGIYKGVIETTFFGKRVYLVGTYSPFYSQQQRN
KSQRILFNDFPKEEFDPTITNPLTSTMLFRTDKDLSLDITLAEKVFSCKDFCEKHKVSCL
ESDMMLLTDSTIVLLQLEQHCSQVNYIPSNNTDTFYPIKDK

Frame A:
---lpkitxhsylsyesfkisltrirsssketyyeshrs*yfkfntndvlnfkidsfimy
qqnqmtiqdgmqlsinfkwlveamvallevsikvllrplslakefiwlvpihhsivnnke
ikvnvyfsmifqrknliqpsqil*qvlcylelikishwillwlkkfslvktfvknikyhv
*nli*cy*livl*fyynwnnivvk*tiyqviipihfiqskik


Frame B:
---fqrlppihtyhmnlskylspeydlqvkkrimratevdtlnsipmmfstsk*irllct
skik*rskmgcsyr*tsngw*rqwwrc*ryl*rcy*dhflwqkslfgwylftil*sttkk
*kstytfq*fskgri*snhhksfnkyyai*n**rsligyyfg*ksfll*rll*kt*simf
ri*ydvin**yysfttigttl*ssklytk**yryilsnqr*


Frame C:
---skdyxpfipii*ifqnishpntifk*rnvl*eppklil*iqyq*CSQLQNRFVYYVP
AKSNDDPRWDAVIDKLQMVGRGNGGAVRGIYKGVIETTFFGKRVYLVGTYSPFYSQQQRN
KSQRILFNDFPKEEFDPTITNPLTSTMLFRTDKDLSLDITLAEKVFSCKDFCEKHKVSCL
ESDMMLLTDSTIVLLQLEQHCSQVNYIPSNNTDTFYPIKDK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO831 (AFO831Q) /CSM/AF/AFO8-B/AFO831Q.Seq.d/                       1298   0.0
AFF364 (AFF364Q) /CSM/AF/AFF3-C/AFF364Q.Seq.d/                       1237   0.0
AHK215 (AHK215Q) /CSM/AH/AHK2-A/AHK215Q.Seq.d/                       1211   0.0
CHD312 (CHD312Q) /CSM/CH/CHD3-A/CHD312Q.Seq.d/                       1172   0.0
AHC412 (AHC412Q) /CSM/AH/AHC4-A/AHC412Q.Seq.d/                       1126   0.0
AFM267 (AFM267Q) /CSM/AF/AFM2-C/AFM267Q.Seq.d/                       1112   0.0
AFK872 (AFK872Q) /CSM/AF/AFK8-C/AFK872Q.Seq.d/                       1104   0.0
AFC207 (AFC207Q) /CSM/AF/AFC2-A/AFC207Q.Seq.d/                       1092   0.0
AFI627 (AFI627Q) /CSM/AF/AFI6-B/AFI627Q.Seq.d/                       1090   0.0
CFG834 (CFG834Q) /CSM/CF/CFG8-B/CFG834Q.Seq.d/                       1084   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC123464|AC123464.4 Rattus norvegicus clone CH230-17P22, WORKING DRAFT SEQUENCE.    46  0.55  1
AI773934|AI773934.1 EST255034 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER8N1, mRNA sequence.    38  0.90  2
BG133859|BG133859.1 EST466751 tomato crown gall Lycopersicon esculentum cDNA clone cTOE14I10 5' sequence, mRNA sequence.    38  1.1  2
AI490786|AI490786.1 EST241494 tomato shoot, Cornell Lycopersicon esculentum cDNA clone cLEB3H9 similar to cinnamic acid 4-hydroxylase, mRNA sequence.    38  1.3  2
BG128254|BG128254.1 EST473900 tomato shoot/meristem Lycopersicon esculentum cDNA clone cTOF19D15 5' sequence, mRNA sequence.    38  1.4  2
AI484136|AI484136.1 EST248943 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER1C5, mRNA sequence.    38  1.6  2
AZ299204|AZ299204.1 RPCI-23-165H3.TV RPCI-23 Mus musculus genomic clone RPCI-23-165H3, DNA sequence.    44  2.2  1
BX323843|BX323843.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-10A23.    46  3.6  3
AC108745|AC108745.7 Homo sapiens 3 BAC RP11-232M24 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    34  3.8  6
AC007361|AC007361.2 Homo sapiens BAC clone RP11-144P23 from 7q11.23-q21.1, complete sequence.    42  5.0  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ812518_2(DQ812518|pid:none) Spironucleus barkhanus isolate Sp1...    39   0.17
Y17877_1(Y17877|pid:none) Sycon raphanus mRNA for insulin recept...    36   0.86
BT011349_1(BT011349|pid:none) Drosophila melanogaster RH02355 fu...    35   1.5
AY069808_1(AY069808|pid:none) Drosophila melanogaster SD04942 fu...    35   1.5
AE014297_4620(AE014297|pid:none) Drosophila melanogaster chromos...    35   1.5
CP001175_2092(CP001175|pid:none) Listeria monocytogenes HCC23, c...    35   2.5
AC009322_3(AC009322|pid:none) Arabidopsis thaliana chromosome I ...    34   3.3
AM270068_10(AM270068|pid:none) Aspergillus niger contig An04c007...    34   4.2
AX814538_1(AX814538|pid:none) Sequence 19 from Patent WO03064471.      33   5.5
AJ010592_91(AJ010592|pid:none) Guillardia theta DNA for complete...    33   5.5

 psg: 0.76  gvh: 0.39  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for AFO831 is nuc

>AFO831Z.Seq
----------TCTTCCAAAGATTACCNCCCATTCATACCTATCATATGAATCTTTCAAAA
TATCTCTCACCCGAATACGATCTTCAAGTAAAGAAACGTATTATGAGAGCCACCGAAGTT
GATACTTTAAATTCAATACCAATGATGTTCTCAACTTCAAAATAGATTCGTTTATTATGT
ACCAGCAAAATCAAATGACGATCCAAGATGGGATGCAGTTATCGATAAACTTCAAATGGT
TGGTAGAGGCAATGGTGGCGCTGTTAGAGGTATCTATAAAGGTGTTATTGAGACCACTTT
CTTTGGCAAAAGAGTTTATTTGGTTGGTACCTATTCACCATTCTATAGTCAACAACAAAG
AAATAAAAGTCAACGTATACTTTTCAATGATTTTCCAAAGGAAGAATTTGATCCAACCAT
CACAAATCCTTTAACAAGTACTATGCTATTTAGAACTGATAAAGATCTCTCATTGGATAT
TACTTTGGCTGAAAAAGTTTTCTCTTGTAAAGACTTTTGTGAAAAACATAAAGTATCATG
TTTAGAATCTGATATGATGTTATTAACTGATAGTACTATAGTTTTACTACAATTGGAACA
ACATTGTAGTCAAGTAAACTATATACCAAGTAATAATACCGATACATTTTATCCAATCAA
AGATAAAG