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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP408
Library VH
(Link to library)
Clone ID VHP408
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHP408P
(Link to Original site)
Representative DNA sequence 
>VHP408 (VHP408Q) /CSM/VH/VHP4-A/VHP408Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATNCCXXXXXXXXXXTCATCCAAAGATGA
TCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCAT
GAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAA
AGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCAC
CAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGT
CACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCG
TGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGT
AGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAG
AGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCA
TTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCA
AGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAA
GAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCC
AGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAA
ANATAGAATCAAATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVX---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*val*vfkxrikskk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslyx---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*val*vfkxrikskk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcx---

---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqf
kshfefsxiesnlkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVX---

---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srtlsfqx*nqi*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP408 (VHP408Q) /CSM/VH/VHP4-A/VHP408Q.Seq.d/                       2656   0.0
VHB613 (VHB613Q) /CSM/VH/VHB6-A/VHB613Q.Seq.d/                       2615   0.0
VHD770 (VHD770Q) /CSM/VH/VHD7-C/VHD770Q.Seq.d/                       1481   0.0
VHA723 (VHA723Q) /CSM/VH/VHA7-A/VHA723Q.Seq.d/                       1481   0.0
VHK821 (VHK821Q) /CSM/VH/VHK8-A/VHK821Q.Seq.d/                       1475   0.0
VHK654 (VHK654Q) /CSM/VH/VHK6-C/VHK654Q.Seq.d/                       1475   0.0
VHK634 (VHK634Q) /CSM/VH/VHK6-B/VHK634Q.Seq.d/                       1475   0.0
VHK494 (VHK494Q) /CSM/VH/VHK4-D/VHK494Q.Seq.d/                       1475   0.0
VHK262 (VHK262Q) /CSM/VH/VHK2-C/VHK262Q.Seq.d/                       1475   0.0
VHK210 (VHK210Q) /CSM/VH/VHK2-A/VHK210Q.Seq.d/                       1475   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445965) Dictyostelium discoideum cDNA clone:ddv61p02, 3' ...  1481   0.0    1
(BJ438848) Dictyostelium discoideum cDNA clone:ddv38l17, 3' ...  1481   0.0    1
(BJ436774) Dictyostelium discoideum cDNA clone:ddv32n05, 3' ...  1481   0.0    1
(BJ443343) Dictyostelium discoideum cDNA clone:ddv52k14, 3' ...  1475   0.0    1
(BJ443220) Dictyostelium discoideum cDNA clone:ddv52c10, 3' ...  1475   0.0    1
(BJ443175) Dictyostelium discoideum cDNA clone:ddv52j06, 3' ...  1475   0.0    1
(BJ443101) Dictyostelium discoideum cDNA clone:ddv51l24, 3' ...  1475   0.0    1
(BJ443012) Dictyostelium discoideum cDNA clone:ddv51k16, 3' ...  1475   0.0    1
(BJ442804) Dictyostelium discoideum cDNA clone:ddv51c04, 3' ...  1475   0.0    1
(BJ442186) Dictyostelium discoideum cDNA clone:ddv49h06, 3' ...  1475   0.0    1
dna update 2008.12.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   1e-80
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   3e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   3e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   297   7e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   9e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   9e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   9e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   296   9e-79
protein update 2009. 7.21
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHP408 is cyt
5' end seq. ID VHP408F
5' end seq. 
>VHP408F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATNCCNNNNNNNNNN
Length of 5' end seq. 646
3' end seq. ID VHP408Z
3' end seq. 
>VHP408Z.Seq
NNNNNNNNNNTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCA
CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGC
CAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTT
CGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAAC
TCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTAT
TTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTC
CCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGA
TTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTC
AATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAG
AAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAAT
TTAAGTCGCACTTTGAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
Length of 3' end seq. 768
Connected seq. ID VHP408P
Connected seq. 
>VHP408P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATNCC----------TCATCCAAAGATGA
TCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCAT
GAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAA
AGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCAC
CAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGT
CACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCG
TGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGT
AGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAG
AGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCA
TTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCA
AGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAA
GAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCC
AGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAA
ANATAGAATCAAATCTAAAAAAAA
Length of connected seq. 1394
Full length Seq ID -
Full length Seq. -
Length of full length seq. -