>VHP305 (VHP305Q) /CSM/VH/VHP3-A/VHP305Q.Seq.d/
XXXXXXXXXXATAAAGTTTTGGCAACCGCTGAAGAGGATGGTATCGTTGAAATGGTGAAT
CCATCAGAGGCGATGGCTTCAGTACTAAGTAAATACGATGGTGACATATTGAAATTCTTT
AAAACTCATAATCCTGACGCTGATTCACCCTATGGTATCGCACCAGAGGTTATGGATACC
TTTGTAAAGAGTTGTGCTGGTTATTGTGTAATCACCTATATTCTTGGTATTGGTGATAGA
CATTTAGATAATCTTTTATTAACTCCAAATGGTAAATTATTCCATATCGATTTCGGTTAC
ATTCTTGGTAAAGATCCAAAGATATTACCACCACCAATGAAACTTTGCAAGGAAATGGTC
TTGGGTATGGGTGGTGAAAATAGTAAACACTATGAAAAATTCAAACAACTCTGTTGCGAA
GCTTATAATATCCTCCGTAAATCTTCTCATTTAATACTCAATCTTTTCGCTCTCATGGTT
GATGCTTCAATACCTTCAATTTCTGATGATAAGGAAA

---KVLATAEEDGIVEMVNPSEAMASVLSKYDGDILKFFKTHNPDADSPYGIAPEVMDTF
VKSCAGYCVITYILGIGDRHLDNLLLTPNGKLFHIDFGYILGKDPKILPPPMKLCKEMVL
GMGGENSKHYEKFKQLCCEAYNILRKSSHLILNLFALMVDASIPSISDDKE

Frame A:
---*sfgnr*rgwyr*ngesirgdgfstk*irw*hieil*ns*s*r*ftlwyrtrgygyl
ckelcwllcnhlyswyw**tfr*sfinskw*iipyrfrlhsw*rskditttnetlqgngl
gygw*k**tl*kiqttllrsl*ypp*ifsfntqsfrshg*cfntfnf***g


Frame B:
---KVLATAEEDGIVEMVNPSEAMASVLSKYDGDILKFFKTHNPDADSPYGIAPEVMDTF
VKSCAGYCVITYILGIGDRHLDNLLLTPNGKLFHIDFGYILGKDPKILPPPMKLCKEMVL
GMGGENSKHYEKFKQLCCEAYNILRKSSHLILNLFALMVDASIPSISDDKE


Frame C:
---kfwqplkrmvslkw*ihqrrwlqy*vntmvty*nslkliiltlihpmvshqrlwipl
*rvvlviv*spiflvlvidi*iify*lqmvnysisisvtflvkiqryyhhq*nfarkwsw
vwvvkivntmknsnnsvakliissvnlli*ysifslswlmlqylqflmirk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP305 (VHP305Q) /CSM/VH/VHP3-A/VHP305Q.Seq.d/                       1005   0.0
CHO259 (CHO259Q) /CSM/CH/CHO2-C/CHO259Q.Seq.d/                       1005   0.0
CHI589 (CHI589Q) /CSM/CH/CHI5-D/CHI589Q.Seq.d/                       1005   0.0
CHH289 (CHH289Q) /CSM/CH/CHH2-D/CHH289Q.Seq.d/                       1005   0.0
CFA858 (CFA858Q) /CSM/CF/CFA8-C/CFA858Q.Seq.d/                       1005   0.0
AHN390 (AHN390Q) /CSM/AH/AHN3-D/AHN390Q.Seq.d/                       1005   0.0
AHB779 (AHB779Q) /CSM/AH/AHB7-D/AHB779Q.Seq.d/                       1005   0.0
AHQ648 (AHQ648Q) /CSM/AH/AHQ6-B/AHQ648Q.Seq.d/                        999   0.0
VHL149 (VHL149Q) /CSM/VH/VHL1-C/VHL149Q.Seq.d/                        997   0.0
VHD748 (VHD748Q) /CSM/VH/VHD7-B/VHD748Q.Seq.d/                        997   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U23480|U23480.1 Dictyostelium discoideum phosphatidylinositol 3-kinase (PIK5) mRNA, complete cds.  1005   0.0    1
AC135425|AC135425.1 Oryza sativa (japonica cultivar-group) chromosome 5 clone P0453H11, *** SEQUENCING IN PROGRESS ***, 6 ordered pieces.    78   1e-10  1
CB667593|CB667593.1 OSJNEd14P22.r OSJNEd Oryza sativa (japonica cultivar-group) cDNA clone OSJNEd14P22 3', mRNA sequence.    78   1e-10  1
CB676144|CB676144.1 OSJNEe12D23.r OSJNEe Oryza sativa (japonica cultivar-group) cDNA clone OSJNEe12D23 3', mRNA sequence.    78   1e-10  1
CB627919|CB627919.1 ---.    78   1e-10  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   5e-07  3
U23478|U23478.1 Dictyostelium discoideum phosphatidylinositol-4,5-diphosphate 3-kinase (PIK3) mRNA, partial cds.    36   6e-07  3
Y09043|Y09043.1 C.albicans VPS34 gene.                             60   3e-05  1
CD005404|CD005404.1 VVB047G11_325362 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB047G11 5, mRNA sequence.    48   6e-05  2
CF208808|CF208808.1 CAB20003_Ia_Ra_A08 Cabernet Sauvignon Flower bloom - CAB2 Vitis vinifera cDNA clone CAB20003_Ia_Ra_A08 3', mRNA sequence.    48   7e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001332_88(CP001332|pid:none) Micromonas sp. RCC299 chromosome ...   219   2e-56
(P42339) RecName: Full=Phosphatidylinositol 3-kinase VPS34;     ...   212   4e-54
U10669_1(U10669|pid:none) Arabidopsis thaliana phosphatidylinosi...   212   4e-54
AY080724_1(AY080724|pid:none) Arabidopsis thaliana putative phos...   212   4e-54
AJ517208_1(AJ517208|pid:none) Medicago truncatula mRNA for phosp...   212   5e-54
DQ118424_1(DQ118424|pid:none) Phaseolus vulgaris phosphatidylino...   211   9e-54
AY142114_1(AY142114|pid:none) Brassica napus phosphatidylinosito...   211   1e-53
DQ118425_1(DQ118425|pid:none) Phaseolus vulgaris phosphatidylino...   210   2e-53
AY701317_1(AY701317|pid:none) Nicotiana tabacum phosphoinositide...   209   3e-53
X99912_1(X99912|pid:none) D.melanogaster mRNA for PI-specific ph...   206   3e-52

 psg: 0.75  gvh: 0.38  alm: 0.32  top: 0.23  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: nuclear
	 20.0 %: vesicles of secretory system
	 16.0 %: cytoplasmic
	 12.0 %: plasma membrane
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHP305 is nuc

>VHP305Z.Seq
NNNNNNNNNNATAAAGTTTTGGCAACCGCTGAAGAGGATGGTATCGTTGAAATGGTGAAT
CCATCAGAGGCGATGGCTTCAGTACTAAGTAAATACGATGGTGACATATTGAAATTCTTT
AAAACTCATAATCCTGACGCTGATTCACCCTATGGTATCGCACCAGAGGTTATGGATACC
TTTGTAAAGAGTTGTGCTGGTTATTGTGTAATCACCTATATTCTTGGTATTGGTGATAGA
CATTTAGATAATCTTTTATTAACTCCAAATGGTAAATTATTCCATATCGATTTCGGTTAC
ATTCTTGGTAAAGATCCAAAGATATTACCACCACCAATGAAACTTTGCAAGGAAATGGTC
TTGGGTATGGGTGGTGAAAATAGTAAACACTATGAAAAATTCAAACAACTCTGTTGCGAA
GCTTATAATATCCTCCGTAAATCTTCTCATTTAATACTCAATCTTTTCGCTCTCATGGTT
GATGCTTCAATACCTTCAATTTCTGATGATAAGGAAA