>AFO816 (AFO816Q) /CSM/AF/AFO8-A/AFO816Q.Seq.d/
XXXXXXXXXXACATCATTGGTAACAATTATGGCAGTTAATAATGAGATTGGTGTAGTTCA
ACCCATTAAAGAGATTGGTAAAATTTGTAGAGAGAATGGTGTTTTCTTCCACACCGATGC
TGCACAAGCAGTTGGTAAGATACCAATAGATGTCAATGATATGAATATTGACCTCCTCTC
GATTAGCGGTCACAAAATATACGGTCCAAAGGGTGTGGGTGCATTATTCGTCAGACGTAG
ACCAAGAGTACGTATCGAGCCAATCACAACTGGTGGTGGTCAAGAACGTGGTATTCGTAG
TGGCACTGTCCCATCGACATTGGCAGTTGGTTTGGGTGCCGCATGTGATATAGCATTGAA
GGAGATGAATCATGATGCCGCTTGGGTTAAATATTTATATGACCGTCTCTTAAAGGGTAT
CACTGATAATATTCCAAATGTTAAAGTAAATGGCGATTTAAATGCACGTTACTATGGTAA
TTTAAATATCTCTTTCTCTTATGTCGAGGGTGAATCCCTTTTAATGGCAATCAAAGATGT
CGCATGTTCAAGTGGTAGTGCCTGTACCTCCTCCTCCTTAGAGCCTTCCTACGTACTTCG
TTCTTTAGGTGTTGAAGAAGATATGGCTCACTCTTCAATTCGTTTCGGTATTGGTCGTTT
CACAACAGAACAAGAAATTGATTACACAATTGAAATCTTAAAAAAGAATGTTCAACGTCT
TAGAGATATGTCACCACTTTGGGA

---SLVTIMAVNNEIGVVQPIKEIGKICRENGVFFHTDAAQAVGKIPIDVNDMNIDLLSI
SGHKIYGPKGVGALFVRRRPRVRIEPITTGGGQERGIRSGTVPSTLAVGLGAACDIALKE
MNHDAAWVKYLYDRLLKGITDNIPNVKVNGDLNARYYGNLNISFSYVEGESLLMAIKDVA
CSSGSACTSSSLEPSYVLRSLGVEEDMAHSSIRFGIGRFTTEQEIDYTIEILKKNVQRLR
DMSPLW

Frame A:
---hhw*qlwqlimrlv*fnplkrlvkfvermvfsstpmlhkqlvryq*msmi*iltssr
lavtkytvqrvwvhyssdvdqeyvssqsqlvvvknvvfvvalshrhwqlvwvphvi*h*r
r*immplglniymtvs*rvsliifqmlk*mai*mhvtmvi*islslmsrvnpf*wqskms
hvqvvvpvpppp*slptyfvl*vlkkiwltlqfvsvlvvsqqnkklitqlks*krmfnvl
eichhfg


Frame B:
---iignnygs***dwcssth*rdw*nl*rewcflphrcctssw*dtnrcq*yey*ppld
*rsqnirskgcgciirqt*tkstyranhnwwwsrtwys*whcpidigswfgcrm*ysieg
des*crlg*ifi*pslkgyh**yskc*skwrfkctllw*fkylfllcrg*ipfngnqrcr
mfkw*clyllllraflrtsffrc*rrygslfnsfrywsfhnrtrn*lhn*nlkkecsts*
ryvttlg


Frame C:
---SLVTIMAVNNEIGVVQPIKEIGKICRENGVFFHTDAAQAVGKIPIDVNDMNIDLLSI
SGHKIYGPKGVGALFVRRRPRVRIEPITTGGGQERGIRSGTVPSTLAVGLGAACDIALKE
MNHDAAWVKYLYDRLLKGITDNIPNVKVNGDLNARYYGNLNISFSYVEGESLLMAIKDVA
CSSGSACTSSSLEPSYVLRSLGVEEDMAHSSIRFGIGRFTTEQEIDYTIEILKKNVQRLR
DMSPLW

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO816 (AFO816Q) /CSM/AF/AFO8-A/AFO816Q.Seq.d/                       1455   0.0
CHJ619 (CHJ619Q) /CSM/CH/CHJ6-A/CHJ619Q.Seq.d/                       1245   0.0
VFD663 (VFD663Q) /CSM/VF/VFD6-C/VFD663Q.Seq.d/                       1080   0.0
CFB659 (CFB659Q) /CSM/CF/CFB6-C/CFB659Q.Seq.d/                       1061   0.0
SFH795 (SFH795Q) /CSM/SF/SFH7-D/SFH795Q.Seq.d/                        971   0.0
SLI840 (SLI840Q) /CSM/SL/SLI8-B/SLI840Q.Seq.d/                        936   0.0
CFH676 (CFH676Q) /CSM/CF/CFH6-D/CFH676Q.Seq.d/                        690   0.0
VSK379 (VSK379Q) /CSM/VS/VSK3-D/VSK379Q.Seq.d/                        668   0.0
FC-IC0015 (FC-IC0015Q) /CSM/FC-IC/FC-IC0015Q.Seq.d/                   266   4e-70
VSK442 (VSK442Q) /CSM/VS/VSK4-B/VSK442Q.Seq.d/                         38   0.19

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL408931|AL408931.1 T7 end of clone AV0AA011E05 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58  1e-13  3
AC135224|AC135224.2 Gallus gallus clone TAM33-46P6, WORKING DRAFT SEQUENCE, 4 ordered pieces.    68  6e-12  2
AJ294714|AJ294714.2 Pichia anomala leu2 gene for beta-isopropylmalate dehydrogenase.    46  1e-11  4
AL407521|AL407521.1 T3 end of clone AV0AA001G08 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    54  3e-10  3
AL430734|AL430734.1 clone BA0AB039F07 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    42  5e-09  4
AX488916|AX488916.1 Sequence 6216 from Patent WO02053728.         38  6e-07  3
BI511982|BI511982.1 BB160007B20B08 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160007B20B08 5', mRNA sequence.    40  3e-06  4
AF000115|AF000115.1 Candida maltosa strain L4 tRNA splicing protein (SPL1) gene, complete cds.    40  3e-06  3
CF401055|CF401055.1 RTWW1_9_F04.g1_A015 Well-watered loblolly pine roots WW1 Pinus taeda cDNA clone RTWW1_9_F04_A015 5', mRNA sequence.    62  1e-05  1
AX685301|AX685301.1 Sequence 108 from Patent WO02066652.          44  3e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ720039_1(AJ720039|pid:none) Gallus gallus mRNA for hypothetica...   307   2e-82
BC134974_1(BC134974|pid:none) Danio rerio NFS1 nitrogen fixation...   305   1e-81
BC142478_1(BC142478|pid:none) Bos taurus NFS1 nitrogen fixation ...   303   4e-81
AJ010952_1(AJ010952|pid:none) Homo sapiens mRNA for putative tRN...   303   5e-81
AK223239_1(AK223239|pid:none) Homo sapiens mRNA for NFS1 nitroge...   303   5e-81
(Q5RDE7) RecName: Full=Cysteine desulfurase, mitochondrial;     ...   302   6e-81
FJ211193_1(FJ211193|pid:none) Sus scrofa nitrogen fixation 1-lik...   301   1e-80
(Q1RHY6) RecName: Full=Cysteine desulfurase;          EC=2.8.1.7...   301   2e-80
AK075575_1(AK075575|pid:none) Mus musculus adult male kidney cDN...   301   2e-80
AK312293_1(AK312293|pid:none) Homo sapiens cDNA, FLJ92597, highl...   301   2e-80

 psg: 0.75  gvh: 0.44  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFO816 is cyt

>AFO816Z.Seq
----------ACATCATTGGTAACAATTATGGCAGTTAATAATGAGATTGGTGTAGTTCA
ACCCATTAAAGAGATTGGTAAAATTTGTAGAGAGAATGGTGTTTTCTTCCACACCGATGC
TGCACAAGCAGTTGGTAAGATACCAATAGATGTCAATGATATGAATATTGACCTCCTCTC
GATTAGCGGTCACAAAATATACGGTCCAAAGGGTGTGGGTGCATTATTCGTCAGACGTAG
ACCAAGAGTACGTATCGAGCCAATCACAACTGGTGGTGGTCAAGAACGTGGTATTCGTAG
TGGCACTGTCCCATCGACATTGGCAGTTGGTTTGGGTGCCGCATGTGATATAGCATTGAA
GGAGATGAATCATGATGCCGCTTGGGTTAAATATTTATATGACCGTCTCTTAAAGGGTAT
CACTGATAATATTCCAAATGTTAAAGTAAATGGCGATTTAAATGCACGTTACTATGGTAA
TTTAAATATCTCTTTCTCTTATGTCGAGGGTGAATCCCTTTTAATGGCAATCAAAGATGT
CGCATGTTCAAGTGGTAGTGCCTGTACCTCCTCCTCCTTAGAGCCTTCCTACGTACTTCG
TTCTTTAGGTGTTGAAGAAGATATGGCTCACTCTTCAATTCGTTTCGGTATTGGTCGTTT
CACAACAGAACAAGAAATTGATTACACAATTGAAATCTTAAAAAAGAATGTTCAACGTCT
TAGAGATATGTCACCACTTTGGGA