>VHP286 (VHP286Q) /CSM/VH/VHP2-D/VHP286Q.Seq.d/
XXXXXXXXXXAACCATGTGGTTTGGTGAGTCTGAAGCCAACGTTCGTGAACTCTTTGATA
AAGCTCGTCAAGCCGCTCCATGTGTTTTGTTCTTTGATGAGTTGGATAGTATTGCACGTT
CTAGAGGTTCATCACAAGGTGATGCTGGTGGTGCTGGTGATAGAGTTATCAATCAAATTT
TAACTGAAATGGATGGTATGAACGCCAAGAAGAACGTTTTCATCATTGGTGCTACCAATA
GACCAGATATCATCGATCCAGCTATCCTCCGTCCAGGTCGTTTAGATCAACTCATTTACA
TTCCATTACCAGATTTACCATCTCGTGTTGCCATTCTCAAGGCTTGTCTCAATAAATCAC
CAGTCGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGACTCAAGGTTTCTCTGGTG
CAGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTATTCGTGAATCCATTGAAA
AGGATATTGAATCCACCAAAGCCCGTCAAGAATCTGGTGATACCAAGATGGAAGATGACT
CTGTCGATCCAGTACCAGAAATTACTCGTGATCACTTCCAAGAAGCCATGAGATCTGCTC
GTCGTTCTGTATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTC
AATCAAGAGGTCTTGGTAATAATTTCAAATTCCCAGATCAAGAATCTCAGGTCAATTCAA
TCAAGNATCACATCNNANNNCTTTCAGA

---TMWFGESEANVRELFDKARQAAPCVLFFDELDSIARSRGSSQGDAGGAGDRVINQIL
TEMDGMNAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDLPSRVAILKACLNKSP
VAKDVDLEFLGQKTQGFSGADLTEICQRACKLAIRESIEKDIESTKARQESGDTKMEDDS
VDPVPEITRDHFQEAMRSARRSVSDNDIRKYESFAQTLVQSRGLGNNFKFPDQESQVNSI
KXHIXXLS

Frame A:
---TMWFGESEANVRELFDKARQAAPCVLFFDELDSIARSRGSSQGDAGGAGDRVINQIL
TEMDGMNAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDLPSRVAILKACLNKSP
VAKDVDLEFLGQKTQGFSGADLTEICQRACKLAIRESIEKDIESTKARQESGDTKMEDDS
VDPVPEITRDHFQEAMRSARRSVSDNDIRKYESFAQTLVQSRGLGNNFKFPDQESQVNSI
KXHIXXLS


Frame B:
---pcglvslkptfvnsliklvkplhvfcslmswivlhvlevhhkvmlvvlvielsikf*
lkwmv*tprrtfsslvlpidqissiqlssvqvv*insftfhyqiyhlvlpfsrlvsinhq
sprm*i*nfwvkrlkvslvqi*lkfvnvpvn*lfvnplkrilnppkpvknlviprwkmtl
siqyqkllvitskkp*dllvvlypimilenmkallkl*fnqevlviisnsqiknlrsiqs
xitsxxfq


Frame C:
---hvvw*v*sqrs*tl**ssssrsmcfvl**vg*yctf*rfitr*cwwcw**syqsnfn
*ngwyerqeerfhhwcyq*tryhrssyppsrsfrsthlhsitrftiscchsqglsq*its
rqgcrfrifgskdsrflwcrfn*nlstcl*isys*ih*kgy*ihqspsriw*yqdgr*lc
rsstrnys*slprsheicssfcir**y*ki*klcsnfssikrsw**fqiprsrisgqfnq
xshxxxfr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP286 (VHP286Q) /CSM/VH/VHP2-D/VHP286Q.Seq.d/                       1429   0.0
CHI521 (CHI521Q) /CSM/CH/CHI5-A/CHI521Q.Seq.d/                       1421   0.0
AHF140 (AHF140Q) /CSM/AH/AHF1-B/AHF140Q.Seq.d/                       1419   0.0
AHE182 (AHE182Q) /CSM/AH/AHE1-D/AHE182Q.Seq.d/                       1419   0.0
AHJ267 (AHJ267Q) /CSM/AH/AHJ2-C/AHJ267Q.Seq.d/                       1415   0.0
AHE345 (AHE345Q) /CSM/AH/AHE3-B/AHE345Q.Seq.d/                       1411   0.0
AHL742 (AHL742Q) /CSM/AH/AHL7-B/AHL742Q.Seq.d/                       1403   0.0
AHC203 (AHC203Q) /CSM/AH/AHC2-A/AHC203Q.Seq.d/                       1398   0.0
AHH538 (AHH538Q) /CSM/AH/AHH5-B/AHH538Q.Seq.d/                       1384   0.0
AHB611 (AHB611Q) /CSM/AH/AHB6-A/AHB611Q.Seq.d/                       1382   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83085|U83085.2 Dictyostelium discoideum cell division cycle protein 48 (cdcD) mRNA, complete cds.  1382   0.0    2
CB814564|CB814564.1 USDA-FP_100042 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-01_D12 5', mRNA sequence.   137   2e-40  5
BE579889|BE579889.1 kq34a03.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:C41C4.8 CE05402 TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE HOMOLOG 2 ;, mRNA sequence.    78   8e-39  4
BI505199|BI505199.1 BB170019B10G02.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170019B10G02 5', mRNA sequence.    76   3e-34  4
BG893512|BG893512.1 kt10d05.y2 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:TER2_CAEEL P54812 TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE HOMOLOG 2 ;, mRNA sequence.    98   4e-34  4
AI067901|AI067901.1 EST209588 Schistosoma mansoni, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMNCG50 5' end similar to transitional endoplasmic reticulum ATPase, mRNA sequence.    82   1e-32  4
BG226420|BG226420.1 kq21d06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:C41C4.8 CE05402 TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE HOMOLOG 2 ;, mRNA sequence.    70   5e-31  3
CD184892|CD184892.1 MS1-0049G-V329-F04-U.B MS1-0049 Schistosoma mansoni cDNA clone MS1-0049G-V329-F04.B, mRNA sequence.    82   8e-30  3
AI067153|AI067153.1 EST208824 Schistosoma mansoni, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMNAQ29 5' end similar to transitional endoplasmic reticulum ATPase, mRNA sequence.    82   3e-29  3
AI067075|AI067075.1 EST208745 Schistosoma mansoni, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMNAC28 5' end similar to transitional endoplasmic reticulum ATPase, mRNA sequence.    82   1e-28  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83085_1(U83085|pid:none) Dictyostelium discoideum cell division...   421   e-116
(P54811) RecName: Full=Transitional endoplasmic reticulum ATPase...   332   1e-89
BC114306_1(BC114306|pid:none) Danio rerio zgc:136908, mRNA (cDNA...   329   6e-89
(P54812) RecName: Full=Transitional endoplasmic reticulum ATPase...   329   6e-89
AK167794_1(AK167794|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   328   1e-88
AK083821_1(AK083821|pid:none) Mus musculus 12 days embryo spinal...   328   1e-88
S25197(S25197;S30329) transitional endoplasmic reticulum ATPase ...   328   1e-88
BC122550_1(BC122550|pid:none) Homo sapiens valosin-containing pr...   328   1e-88
AL137377_1(AL137377|pid:none) Homo sapiens mRNA; cDNA DKFZp434K0...   328   1e-88
(P03974) RecName: Full=Transitional endoplasmic reticulum ATPase...   328   1e-88

 psg: 0.75  gvh: 0.46  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHP286 is cyt

>VHP286Z.Seq
NNNNNNNNNNAACCATGTGGTTTGGTGAGTCTGAAGCCAACGTTCGTGAACTCTTTGATA
AAGCTCGTCAAGCCGCTCCATGTGTTTTGTTCTTTGATGAGTTGGATAGTATTGCACGTT
CTAGAGGTTCATCACAAGGTGATGCTGGTGGTGCTGGTGATAGAGTTATCAATCAAATTT
TAACTGAAATGGATGGTATGAACGCCAAGAAGAACGTTTTCATCATTGGTGCTACCAATA
GACCAGATATCATCGATCCAGCTATCCTCCGTCCAGGTCGTTTAGATCAACTCATTTACA
TTCCATTACCAGATTTACCATCTCGTGTTGCCATTCTCAAGGCTTGTCTCAATAAATCAC
CAGTCGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGACTCAAGGTTTCTCTGGTG
CAGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTATTCGTGAATCCATTGAAA
AGGATATTGAATCCACCAAAGCCCGTCAAGAATCTGGTGATACCAAGATGGAAGATGACT
CTGTCGATCCAGTACCAGAAATTACTCGTGATCACTTCCAAGAAGCCATGAGATCTGCTC
GTCGTTCTGTATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTC
AATCAAGAGGTCTTGGTAATAATTTCAAATTCCCAGATCAAGAATCTCAGGTCAATTCAA
TCAAGNATCACATCNNANNNCTTTCAGA