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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO814
Library AF
(Link to library)
Clone ID AFO814
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16518-1
Original site URL
Representative seq. ID AFO814P
(Link to Original site)
Representative DNA sequence 
>AFO814 (AFO814Q) /CSM/AF/AFO8-A/AFO814Q.Seq.d/
AATAAATTCAATTATTCATATATATATACATATACACTATTATAATTCTAATTTTATTAA
TATTTTAATCTCTTTCATTTTTTTAAATTTTATTTTTTAAATTCTATTTTTCATTTTTTT
TCTATAAATTTTTTATTTTTAAAACTTTTTATTTTATTTCCAAAAAAAAAAXXXXXXXXX
XTAATGCATCAAAANAACTCTTCCAAGAGTTTTTACAATCACATTTAATTGAATCTCTGA
TATCAATTTCGCACCAAATGAANCAATTGTATCATTAAAAGATTCTGAAAATTATCTTAA
AACAATGAAATTAACTGGTACTAAATTTAATGATTCTCAAATTACTGTTCTTAATTATAC
AAGAAAACAACCAGGCTTTGTTCCACCAAGTTATTCAAATCAATCATCATCATCATCAGG
TGATAATCAATCATTCCAACCACAATCAACTTCTAAAGTTAATCCATTTGGTAATGCTAC
TCCAATTGATACTTCTAAAGTTCATCTTTCAAATTCTACTCAACCACAACAACCAACTCA
ACAACAACCACAACCAACCCAACAACAACAGCAACAACAACAACCAACTCAACAAGGTCA
ACAAGGTCAACAACAACCACAACAACAAAATGATAATAAATACAGACCAAACTCAATGAG
AACTAATAGTGGATTCAAACAAAATGACAATAAATTTGATAACAACAACAAATTTGATAA
CAAGAAACCATTTGGACAAAATAAATACAACAACAAGAGAGAAGAAGATGGTTGGAAATC
AACTGGTCCAGCAAGACATCCAAAAGATCAACCATCAAAAGAAGTATCACAACCAACTGT
TGCCAAGACTTCGGAAATTTTTCGAANTTTAGAACAAGACGATTAACAAATCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nkfnysyiytytll*f*fy*YFNLFHFFKFYFLNSIFHFFSINFLFLKLFILFPKKK---

---NASKXLFQEFLQSHLIESLISISHQMXQLYH*kilkiilkq*n*lvlnlmilkllfl
iiqennqalfhqviqinhhhhqviinhsnhnqllklihlvmllqlillkfifqillnhnn
qlnnnhnqpnnnsnnnnqlnkvnkvnnnhnnkmiintdqtq*elivdsnkmtinlitttn
litrnhldkintttrekkmvgnqlvqqdiqkinhqkkyhnqllprlrkffex*nktinks
Translated Amino Acid sequence (All Frames) 
Frame A:
nkfnysyiytytll*f*fy*YFNLFHFFKFYFLNSIFHFFSINFLFLKLFILFPKKK---

---*cikxtlprvftitfn*isdinfapnexivslkdsenylktmkltgtkfndsqitvl
nytrkqpgfvppsysnqsssssgdnqsfqpqstskvnpfgnatpidtskvhlsnstqpqq
ptqqqpqptqqqqqqqqptqqgqqgqqqpqqqndnkyrpnsmrtnsgfkqndnkfdnnnk
fdnkkpfgqnkynnkreedgwkstgparhpkdqpskevsqptvaktseifrxleqdd*qi

Frame B:
insiihiyihihyynsnfinilisfiflnfif*ilffiffl*ifyf*nflfyfqkk---

---NASKXLFQEFLQSHLIESLISISHQMXQLYH*kilkiilkq*n*lvlnlmilkllfl
iiqennqalfhqviqinhhhhqviinhsnhnqllklihlvmllqlillkfifqillnhnn
qlnnnhnqpnnnsnnnnqlnkvnkvnnnhnnkmiintdqtq*elivdsnkmtinlitttn
litrnhldkintttrekkmvgnqlvqqdiqkinhqkkyhnqllprlrkffex*nktinks

Frame C:
*iqlfiyiyiytiiilillif*slsff*ilffkfyfsfffykffifktfyfiskkk---

---mhqxnssksfynhi*lnl*yqfrtk*xnciikrf*kls*nneinwy*i**fsnycs*
lykkttrlcstklfksiiiiir**siipttinf*s*siw*cysn*yf*sssfkfystttt
nstttttnptttattttnstrstrsttttttk***iqtklnen**wiqtk*q*i**qqqi
**qetiwtk*iqqqerrrwleinwssktskrstikrsittnccqdfgnfsxfrtrrltn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO814 (AFO814Q) /CSM/AF/AFO8-A/AFO814Q.Seq.d/                        519   e-146
AFD886 (AFD886Q) /CSM/AF/AFD8-D/AFD886Q.Seq.d/                        507   e-143
VFA155 (VFA155Q) /CSM/VF/VFA1-C/VFA155Q.Seq.d/                        478   e-134
VFI629 (VFI629Q) /CSM/VF/VFI6-B/VFI629Q.Seq.d/                        464   e-129
SLC443 (SLC443Q) /CSM/SL/SLC4-B/SLC443Q.Seq.d/                        464   e-129
AFH480 (AFH480Q) /CSM/AF/AFH4-D/AFH480Q.Seq.d/                        464   e-129
AFE677 (AFE677Q) /CSM/AF/AFE6-D/AFE677Q.Seq.d/                        464   e-129
AFC880 (AFC880Q) /CSM/AF/AFC8-D/AFC880Q.Seq.d/                        464   e-129
AFI776 (AFI776Q) /CSM/AF/AFI7-D/AFI776Q.Seq.d/                        462   e-129
SLH467 (SLH467Q) /CSM/SL/SLH4-C/SLH467Q.Seq.d/                        456   e-127
own update 2001.11.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AY065265|AY065265.1 Arabidopsis thaliana unknown protein (At2g31955) mRNA, complete cds.    48  0.19  1
AV807473|AV807473.1 Arabidopsis thaliana cDNA clone:RAFL09-50-J04, 3'-end.    48  0.19  1
AY087242|AY087242.1 Arabidopsis thaliana clone 33192 mRNA, complete sequence.    48  0.19  1
AV804136|AV804136.1 Arabidopsis thaliana cDNA clone:RAFL09-38-A15, 3'-end.    48  0.19  1
AC006533|AC006533.8 Arabidopsis thaliana chromosome 2 clone F20M17 map nga361, complete sequence.    48  0.44  2
Z48047|Z48047.1 A.thaliana mRNA for molybdenum cofactor biosynthesis Cnx2 protein.    46  0.75  1
AC110972|AC110972.5 Rattus norvegicus clone CH230-71J5, WORKING DRAFT SEQUENCE, 2 unordered pieces.    46  0.75  1
AC110973|AC110973.5 Rattus norvegicus clone CH230-111K9, WORKING DRAFT SEQUENCE, 5 unordered pieces.    46  0.75  1
AC098603|AC098603.7 Rattus norvegicus clone CH230-139E21, WORKING DRAFT SEQUENCE, 2 unordered pieces.    46  0.75  1
BH147769|BH147769.1 ENTPH48TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  1.2  2
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC135220_1(BC135220|pid:none) Xenopus tropicalis tyrosine kinase...    36   1.4
protein update 2008.11.27
PSORT 

 psg: 0.93  gvh: 0.41  alm: 0.29  top: 0.73  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFO814 is nuc
5' end seq. ID AFO814F
5' end seq. 
>AFO814F.Seq
AATAAATTCAATTATTCATATATATATACATATACACTATTATAATTCTAATTTTATTAA
TATTTTAATCTCTTTCATTTTTTTAAATTTTATTTTTTAAATTCTATTTTTCATTTTTTT
TCTATAAATTTTTTATTTTTAAAACTTTTTATTTTATTTCCAAAAAAAAAA---------
-
Length of 5' end seq. 171
3' end seq. ID AFO814Z
3' end seq. 
>AFO814Z.Seq
----------TAATGCATCAAAANAACTCTTCCAAGAGTTTTTACAATCACATTTAATTG
AATCTCTGATATCAATTTCGCACCAAATGAANCAATTGTATCATTAAAAGATTCTGAAAA
TTATCTTAAAACAATGAAATTAACTGGTACTAAATTTAATGATTCTCAAATTACTGTTCT
TAATTATACAAGAAAACAACCAGGCTTTGTTCCACCAAGTTATTCAAATCAATCATCATC
ATCATCAGGTGATAATCAATCATTCCAACCACAATCAACTTCTAAAGTTAATCCATTTGG
TAATGCTACTCCAATTGATACTTCTAAAGTTCATCTTTCAAATTCTACTCAACCACAACA
ACCAACTCAACAACAACCACAACCAACCCAACAACAACAGCAACAACAACAACCAACTCA
ACAAGGTCAACAAGGTCAACAACAACCACAACAACAAAATGATAATAAATACAGACCAAA
CTCAATGAGAACTAATAGTGGATTCAAACAAAATGACAATAAATTTGATAACAACAACAA
ATTTGATAACAAGAAACCATTTGGACAAAATAAATACAACAACAAGAGAGAAGAAGATGG
TTGGAAATCAACTGGTCCAGCAAGACATCCAAAAGATCAACCATCAAAAGAAGTATCACA
ACCAACTGTTGCCAAGACTTCGGAAATTTTTCGAANTTTAGAACAAGACGATTAACAAAT
CA
Length of 3' end seq. 712
Connected seq. ID AFO814P
Connected seq. 
>AFO814P.Seq
AATAAATTCAATTATTCATATATATATACATATACACTATTATAATTCTAATTTTATTAA
TATTTTAATCTCTTTCATTTTTTTAAATTTTATTTTTTAAATTCTATTTTTCATTTTTTT
TCTATAAATTTTTTATTTTTAAAACTTTTTATTTTATTTCCAAAAAAAAAA---------
-TAATGCATCAAAANAACTCTTCCAAGAGTTTTTACAATCACATTTAATTGAATCTCTGA
TATCAATTTCGCACCAAATGAANCAATTGTATCATTAAAAGATTCTGAAAATTATCTTAA
AACAATGAAATTAACTGGTACTAAATTTAATGATTCTCAAATTACTGTTCTTAATTATAC
AAGAAAACAACCAGGCTTTGTTCCACCAAGTTATTCAAATCAATCATCATCATCATCAGG
TGATAATCAATCATTCCAACCACAATCAACTTCTAAAGTTAATCCATTTGGTAATGCTAC
TCCAATTGATACTTCTAAAGTTCATCTTTCAAATTCTACTCAACCACAACAACCAACTCA
ACAACAACCACAACCAACCCAACAACAACAGCAACAACAACAACCAACTCAACAAGGTCA
ACAAGGTCAACAACAACCACAACAACAAAATGATAATAAATACAGACCAAACTCAATGAG
AACTAATAGTGGATTCAAACAAAATGACAATAAATTTGATAACAACAACAAATTTGATAA
CAAGAAACCATTTGGACAAAATAAATACAACAACAAGAGAGAAGAAGATGGTTGGAAATC
AACTGGTCCAGCAAGACATCCAAAAGATCAACCATCAAAAGAAGTATCACAACCAACTGT
TGCCAAGACTTCGGAAATTTTTCGAANTTTAGAACAAGACGATTAACAAATCA
Length of connected seq. 883
Full length Seq ID -
Full length Seq. -
Length of full length seq. -