>AFO812 (AFO812Q) /CSM/AF/AFO8-A/AFO812Q.Seq.d/
ATAATGATAATAATAATAATGATAATAATAATACTATTACAAATAGCCATATAAATAATC
ACGATCATAAACATAATCATGAACACGAACATAAACATAAACATAATCACGATCATAATC
ATGACCATGACCATAACCATGAAGAAGAATATGGACATGGAAATGAACTGGAACACAACA
ATGACCAAGAACATAATGTTGGTAATAAAAATTTATTAACAAATAATAATAATCAAAGTA
AAAAGAAAAAACATGGACATTCACATGGAGGTGGTGAAGATGGTAGTAGTAGTGGTGGTG
GTTGGGTGGTCGACATGGACATGGACATTCACATGGTGGTGGTAGTGGTAGTGATCATAA
TCATGGATCATCAGATGAAGACGATGAAGAAAGTAAACCATTAAATCAATTAAGAAATTT
AGATAGTAAAAAGAAAGCAAGATATTCATTGATATTAGCATTAACATTGACAACAATATT
TATGGNTGGTGAAATTGTAGGTGGTTATTTTGCAAATTCATTGGCAATTATGACGGATGC
AGCTCATTTGTTAACGGATATTGGTGCAATGTTTTTATCATTATTTGCAATGNGGATTAG
NCAACATCCACCAACAAGTTCAATGAGTTTTGGATTTCATCGXXXXXXXXXXGGAAAGAT
TATGTTTATCATTGCATCATGTGGTTTATTTATCAACATTATAGATGCCATCATTTTACA
TTGGGGTTCGGGCGGTCATGGCCATTCCCATGGTGGTGGCCATGGTCATTCTCATGGCAT
TGGCGGAGGAACTCAAAAAAAGAAGAGTAAAAAGAATAGATTATTAAATAATCAAGGTCA
AGATATAGAAGATTTAGGTGGTGAAAATGGTAAAAACAAAAAAGGTGTAAGAAATATCAA
TGTCCATTCAGCATATATTCATGTATTGGGTGACTGTTTTCAAAGTATTGGTGTCATGGT
GGCAAGTTGTATCATTTGGGTTCACCCTCATTGGAAGATTGCAGATCCAATCACTACATT
GATTTTCTCTGTAATTGTATTGGGAACAACTATTAAATTGTTACGTGAAAGTTTAGGTGT
ACTCATGGAGGGTGTACCACCAGAAATTGACGTTTCAGAAGTTAAAGGTGATCTCTCAGA
GATTGAAGGTGTAACAGAGGTACATGACCTTCATATTTGGTCAATCACTCTTGGTAGACC
AGCTCTAAGCGTTCATTTAACAATCTTACCAACAATAGATCCCGAAGAAATCTTATCAAT
TGCAAATAAAATCTTATTAGAAGATTATGCCAATTAATCATACAACAATTCAAATTGAAA
AACCATTAGTTAAAGATAAATGTAAAGATCATAGTTGTCCACCTCCAAAACCAAAAAAAA
AAAAACATTAAAAANA

**********yyyk*pyk*srs*t*s*trt*t*t*srs*s*p*p*p*rriwtwk*tgtqq
*prt*cw**kfink***sk*kektwtftwrw*rw***WWWLGGRHGHGHSHGGGSGSDHN
HGSSDEDDEESKPLNQLRNLDSKKKARYSLILALTLTTIFMXGEIVGGYFANSLAIMTDA
AHLLTDIGAMFLSLFAMXIXQHPPTSSMSFGFH---

---GKIMFIIASCGLFINIIDAIILHWGSGGHGHSHGGGHGHSHGIGGGTQKKKSKKNRL
LNNQGQDIEDLGGENGKNKKGVRNINVHSAYIHVLGDCFQSIGVMVASCIIWVHPHWKIA
DPITTLIFSVIVLGTTIKLLRESLGVLMEGVPPEIDVSEVKGDLSEIEGVTEVHDLHIWS
ITLGRPALSVHLTILPTIDPEEILSIANKILLEDYAN*synnsn*ktis*r*m*rs*lst
sktkkkktlkx

Frame A:
imiiiimiiiillqiai*iitiiniimntnininiitiiimtmtitmkknmdmemnwntt
mtknimlvikiy*qiiiikvkrknmdihmevvkmvvvvvvvgwstwtwtftwww*w**s*
swiir*rr*rk*tiksikkfr**keskifidisinidnniygw*ncrwlfckfignydgc
ssfvngywcnvfiiicnxd*xtstnkfnefwiss---

---GKIMFIIASCGLFINIIDAIILHWGSGGHGHSHGGGHGHSHGIGGGTQKKKSKKNRL
LNNQGQDIEDLGGENGKNKKGVRNINVHSAYIHVLGDCFQSIGVMVASCIIWVHPHWKIA
DPITTLIFSVIVLGTTIKLLRESLGVLMEGVPPEIDVSEVKGDLSEIEGVTEVHDLHIWS
ITLGRPALSVHLTILPTIDPEEILSIANKILLEDYAN*synnsn*ktis*r*m*rs*lst
sktkkkktlkx

Frame B:
**********yyyk*pyk*srs*t*s*trt*t*t*srs*s*p*p*p*rriwtwk*tgtqq
*prt*cw**kfink***sk*kektwtftwrw*rw***WWWLGGRHGHGHSHGGGSGSDHN
HGSSDEDDEESKPLNQLRNLDSKKKARYSLILALTLTTIFMXGEIVGGYFANSLAIMTDA
AHLLTDIGAMFLSLFAMXIXQHPPTSSMSFGFH---

---erlclslhhvvylstl*mpsfyigvravmaipmvvamvilmalaeelkkrrvkridy
*iikvki*ki*vvkmvktkkv*eismsiqhifmywvtvfkvlvswwqvvsfgftligrlq
iqslh*fsl*lyweqllncyvkv*vyswrvyhqkltfqklkvisqrlkv*qrymtfifgq
sllvdql*afi*qsyqq*ipkksyqlqiksy*kimpinhttiqiekplvkdkckdhscpp
pkpkkkkh*k

Frame C:
ndnnnndnnntitnshinnhdhkhnhehehkhkhnhdhnhdhdhnheeeyghgnelehnn
dqehnvgnknlltnnnnqskkkkhghshgggedgsssgggwvvdmdmdihmvvvvvviii
mdhqmktmkkvnh*in*ei*ivkrkqdih*y*h*h*qqylwxvkl*vvilqihwql*rmq
lic*rilvqcfyhylqxglxnihqqvq*vldfi---

---kdyvyhcimwfiyqhyrchhftlgfgrswpfpwwwpwsfswhwrrnskkee*ke*ii
k*srsryrrfrw*kw*kqkrckkyqcpfsiyscig*lfskywchggklyhlgspsledcr
snhyidflcncignny*ivt*kfrcthggcttrn*rfrs*r*slrd*rcnrgt*psylvn
hsw*tsskrsfnnltnnrsrrnlinck*nlirrlcqliiqqfklknh*lkinvkiivvhl
qnqkkknikx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO812 (AFO812Q) /CSM/AF/AFO8-A/AFO812Q.Seq.d/                       1850   0.0
SFB468 (SFB468Q) /CSM/SF/SFB4-C/SFB468Q.Seq.d/                       1047   0.0
SLC791 (SLC791Q) /CSM/SL/SLC7-D/SLC791Q.Seq.d/                        946   0.0
SLC553 (SLC553Q) /CSM/SL/SLC5-C/SLC553Q.Seq.d/                        946   0.0
SSH419 (SSH419Q) /CSM/SS/SSH4-A/SSH419Q.Seq.d/                        819   0.0
VHJ618 (VHJ618Q) /CSM/VH/VHJ6-A/VHJ618Q.Seq.d/                         44   0.006
VHC770 (VHC770Q) /CSM/VH/VHC7-C/VHC770Q.Seq.d/                         44   0.006
CHM623 (CHM623Q) /CSM/CH/CHM6-A/CHM623Q.Seq.d/                         44   0.006
VFM422 (VFM422Q) /CSM/VF/VFM4-A/VFM422Q.Seq.d/                         42   0.023
SSC560 (SSC560Q) /CSM/SS/SSC5-C/SSC560Q.Seq.d/                         42   0.023

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM275637|BM275637.1 PfESToaa55e12.y1 Plasmodium falciparum 3D7 gametocyte cDNA library Plasmodium falciparum 3D7 cDNA 5' similar to TR:Q69566 Q69566 ;, mRNA sequence.    66  1e-08  2
AE014818|AE014818.1 Plasmodium falciparum 3D7 chromosome 14 section 3 of 13 of the complete sequence.    66  1e-06  3
BG135345|BG135345.1 EST468237 tomato crown gall Lycopersicon esculentum cDNA clone cTOE22E18 5' sequence, mRNA sequence.    54  0.005  1
AW625482|AW625482.1 EST319389 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ15O13 5', mRNA sequence.    54  0.005  1
AE014846|AE014846.1 Plasmodium falciparum 3D7 chromosome 12, section 3 of 9 of the complete sequence.    50  0.076  1
AF053125|AF053125.1 Haemophilus influenzae putative adhesin B precursor FimA gene, partial cds.    50  0.076  1
AL472594|AL472594.1 T. brucei sheared genomic DNA clone 156f04, reverse sequence.    50  0.076  1
AC104197|AC104197.5 Mus musculus clone RP24-319N16, WORKING DRAFT SEQUENCE, 8 unordered pieces.    50  0.076  1
AL497301|AL497301.1 T. brucei sheared genomic DNA clone 327f02, forward sequence.    50  0.076  1
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.          50  0.076  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC163234_1(BC163234|pid:none) Danio rerio solute carrier family ...   195   4e-48
BC147704_1(BC147704|pid:none) Mus musculus solute carrier family...   191   4e-47
BC113751_1(BC113751|pid:none) Mus musculus solute carrier family...   191   5e-47
BC061997_1(BC061997|pid:none) Rattus norvegicus solute carrier f...   191   5e-47
(Q62941) RecName: Full=Zinc transporter 2;          Short=ZnT-2;...   191   5e-47
Z68119_8(Z68119|pid:none) Caenorhabditis elegans Cosmid T18D3, c...   190   1e-46
AY483148_1(AY483148|pid:none) Arabidopsis lyrata heavy metal tra...   189   2e-46
BC076884_1(BC076884|pid:none) Xenopus tropicalis solute carrier ...   189   2e-46
AY188451_1(AY188451|pid:none) Brassica juncea cation-efflux tran...   187   6e-46
BC071120_1(BC071120|pid:none) Xenopus laevis MGC81386 protein, m...   187   7e-46

 psg: 0.78  gvh: 0.39  alm: 0.24  top: 0.20  tms: 0.33  mit: 0.20  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: plasma membrane
	 28.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  8.0 %: nuclear
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFO812 is pla

>AFO812F.Seq
ATAATGATAATAATAATAATGATAATAATAATACTATTACAAATAGCCATATAAATAATC
ACGATCATAAACATAATCATGAACACGAACATAAACATAAACATAATCACGATCATAATC
ATGACCATGACCATAACCATGAAGAAGAATATGGACATGGAAATGAACTGGAACACAACA
ATGACCAAGAACATAATGTTGGTAATAAAAATTTATTAACAAATAATAATAATCAAAGTA
AAAAGAAAAAACATGGACATTCACATGGAGGTGGTGAAGATGGTAGTAGTAGTGGTGGTG
GTTGGGTGGTCGACATGGACATGGACATTCACATGGTGGTGGTAGTGGTAGTGATCATAA
TCATGGATCATCAGATGAAGACGATGAAGAAAGTAAACCATTAAATCAATTAAGAAATTT
AGATAGTAAAAAGAAAGCAAGATATTCATTGATATTAGCATTAACATTGACAACAATATT
TATGGNTGGTGAAATTGTAGGTGGTTATTTTGCAAATTCATTGGCAATTATGACGGATGC
AGCTCATTTGTTAACGGATATTGGTGCAATGTTTTTATCATTATTTGCAATGNGGATTAG
NCAACATCCACCAACAAGTTCAATGAGTTTTGGATTTCATCG----------

>AFO812Z.Seq
----------GGAAAGATTATGTTTATCATTGCATCATGTGGTTTATTTATCAACATTAT
AGATGCCATCATTTTACATTGGGGTTCGGGCGGTCATGGCCATTCCCATGGTGGTGGCCA
TGGTCATTCTCATGGCATTGGCGGAGGAACTCAAAAAAAGAAGAGTAAAAAGAATAGATT
ATTAAATAATCAAGGTCAAGATATAGAAGATTTAGGTGGTGAAAATGGTAAAAACAAAAA
AGGTGTAAGAAATATCAATGTCCATTCAGCATATATTCATGTATTGGGTGACTGTTTTCA
AAGTATTGGTGTCATGGTGGCAAGTTGTATCATTTGGGTTCACCCTCATTGGAAGATTGC
AGATCCAATCACTACATTGATTTTCTCTGTAATTGTATTGGGAACAACTATTAAATTGTT
ACGTGAAAGTTTAGGTGTACTCATGGAGGGTGTACCACCAGAAATTGACGTTTCAGAAGT
TAAAGGTGATCTCTCAGAGATTGAAGGTGTAACAGAGGTACATGACCTTCATATTTGGTC
AATCACTCTTGGTAGACCAGCTCTAAGCGTTCATTTAACAATCTTACCAACAATAGATCC
CGAAGAAATCTTATCAATTGCAAATAAAATCTTATTAGAAGATTATGCCAATTAATCATA
CAACAATTCAAATTGAAAAACCATTAGTTAAAGATAAATGTAAAGATCATAGTTGTCCAC
CTCCAAAACCAAAAAAAAAAAAACATTAAAAANA

>AFO812P.Seq
ATAATGATAATAATAATAATGATAATAATAATACTATTACAAATAGCCATATAAATAATC
ACGATCATAAACATAATCATGAACACGAACATAAACATAAACATAATCACGATCATAATC
ATGACCATGACCATAACCATGAAGAAGAATATGGACATGGAAATGAACTGGAACACAACA
ATGACCAAGAACATAATGTTGGTAATAAAAATTTATTAACAAATAATAATAATCAAAGTA
AAAAGAAAAAACATGGACATTCACATGGAGGTGGTGAAGATGGTAGTAGTAGTGGTGGTG
GTTGGGTGGTCGACATGGACATGGACATTCACATGGTGGTGGTAGTGGTAGTGATCATAA
TCATGGATCATCAGATGAAGACGATGAAGAAAGTAAACCATTAAATCAATTAAGAAATTT
AGATAGTAAAAAGAAAGCAAGATATTCATTGATATTAGCATTAACATTGACAACAATATT
TATGGNTGGTGAAATTGTAGGTGGTTATTTTGCAAATTCATTGGCAATTATGACGGATGC
AGCTCATTTGTTAACGGATATTGGTGCAATGTTTTTATCATTATTTGCAATGNGGATTAG
NCAACATCCACCAACAAGTTCAATGAGTTTTGGATTTCATCG----------GGAAAGAT
TATGTTTATCATTGCATCATGTGGTTTATTTATCAACATTATAGATGCCATCATTTTACA
TTGGGGTTCGGGCGGTCATGGCCATTCCCATGGTGGTGGCCATGGTCATTCTCATGGCAT
TGGCGGAGGAACTCAAAAAAAGAAGAGTAAAAAGAATAGATTATTAAATAATCAAGGTCA
AGATATAGAAGATTTAGGTGGTGAAAATGGTAAAAACAAAAAAGGTGTAAGAAATATCAA
TGTCCATTCAGCATATATTCATGTATTGGGTGACTGTTTTCAAAGTATTGGTGTCATGGT
GGCAAGTTGTATCATTTGGGTTCACCCTCATTGGAAGATTGCAGATCCAATCACTACATT
GATTTTCTCTGTAATTGTATTGGGAACAACTATTAAATTGTTACGTGAAAGTTTAGGTGT
ACTCATGGAGGGTGTACCACCAGAAATTGACGTTTCAGAAGTTAAAGGTGATCTCTCAGA
GATTGAAGGTGTAACAGAGGTACATGACCTTCATATTTGGTCAATCACTCTTGGTAGACC
AGCTCTAAGCGTTCATTTAACAATCTTACCAACAATAGATCCCGAAGAAATCTTATCAAT
TGCAAATAAAATCTTATTAGAAGATTATGCCAATTAATCATACAACAATTCAAATTGAAA
AACCATTAGTTAAAGATAAATGTAAAGATCATAGTTGTCCACCTCCAAAACCAAAAAAAA
AAAAACATTAAAAANA