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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP223
Library VH
(Link to library)
Clone ID VHP223
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15897-1
Original site URL
Representative seq. ID VHP223P
(Link to Original site)
Representative DNA sequence 
>VHP223 (VHP223Q) /CSM/VH/VHP2-A/VHP223Q.Seq.d/
TCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTATTA
AAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATGGAA
GTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCACGTA
CTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAACAAT
TAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAATGT
CAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACATTAA
CATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTTATT
ATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATCATA
TTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAGXXX
XXXXXXXCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTTGTA
ATTGGTGAACTTCCAGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGATCC
AAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTT
GGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTT
AATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAA
TGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGT
TCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGA
TGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAAT
CTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCAC
TGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCT
TTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTAT
GCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGG
TGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
NEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKPRT
FGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITTLT
SPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE--
-

---IDEAVELAQSSDVVVVVIGELPEAETPG*yl*slygsk*stlittig*hw*tssfnf
g*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrc
silpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhch
chkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*illyw
cr**nyfrigqfii
Translated Amino Acid sequence (All Frames) 
Frame A:
smni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslknhv
lleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwilqh*
hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkpllsk-
--

---qsmkplnwlnhqmllsl*lvnfqklklqgdiydlsmdpnevlllqqlvdtgkpvvli
lvearprilppdlvyscaavlmaylpgseggkpianilmgnvnpsgrlpltypgttgdig
vpyyhkysengvttplfqfgdglsyttfnytnlacsnckpisgqsgnytgvlgqsytftv
tvtnngnvqgkdsvllylsdlwaqvtpevkmlrgfqkvdlmpaksqqisftlnayefsfi
gvdnkitlesvnssl

Frame B:
q*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*ktty
fwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyynin
itkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnhcyr--
-

---nr*sr*igsiircccrcnw*tsrs*nsrvifmislwiqmkysyynnwltlvnq*f*f
wlklvqefyhqisstvvlqf*wlisqvvkvvnqsptfswvm*ihlvvfl*liqvllvisv
fhittntvkmvsplhssnlvmvfliplltipispvaivnqslvnlvttlvf*vnpilsls
lsqtmvtfkvktlfsyislifglkllqklkc*evskklilcqlnlnksllpsmpmnspll
vsiikll*nrsihh

Frame C:
NEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKPRT
FGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITTLT
SPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE--
-

---IDEAVELAQSSDVVVVVIGELPEAETPG*yl*slygsk*stlittig*hw*tssfnf
g*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrc
silpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhch
chkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*illyw
cr**nyfrigqfii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP223 (VHP223Q) /CSM/VH/VHP2-A/VHP223Q.Seq.d/                       2440   0.0
VHI472 (VHI472Q) /CSM/VH/VHI4-C/VHI472Q.Seq.d/                       2418   0.0
VHD112 (VHD112Q) /CSM/VH/VHD1-A/VHD112Q.Seq.d/                       2418   0.0
VHA656 (VHA656Q) /CSM/VH/VHA6-C/VHA656Q.Seq.d/                       1493   0.0
VHA583 (VHA583Q) /CSM/VH/VHA5-D/VHA583Q.Seq.d/                       1483   0.0
VHA533 (VHA533Q) /CSM/VH/VHA5-B/VHA533Q.Seq.d/                       1483   0.0
CHS391 (CHS391Q) /CSM/CH/CHS3-D/CHS391Q.Seq.d/                       1483   0.0
CHL451 (CHL451Q) /CSM/CH/CHL4-C/CHL451Q.Seq.d/                       1483   0.0
CHF295 (CHF295Q) /CSM/CH/CHF2-D/CHF295Q.Seq.d/                       1483   0.0
VHJ810 (VHJ810Q) /CSM/VH/VHJ8-A/VHJ810Q.Seq.d/                       1481   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445955) Dictyostelium discoideum cDNA clone:ddv61m06, 3' ...  1499   0.0    1
(BJ436938) Dictyostelium discoideum cDNA clone:ddv32o14, 3' ...  1493   0.0    1
(BJ438020) Dictyostelium discoideum cDNA clone:ddv36i03, 3' ...  1360   0.0    1
(BJ442826) Dictyostelium discoideum cDNA clone:ddv51h02, 3' ...  1346   0.0    1
(BJ441592) Dictyostelium discoideum cDNA clone:ddv47o05, 3' ...  1334   0.0    2
(BJ446635) Dictyostelium discoideum cDNA clone:ddv63i07, 3' ...  1332   0.0    2
(BJ446488) Dictyostelium discoideum cDNA clone:ddv62k19, 3' ...  1332   0.0    2
(BJ444816) Dictyostelium discoideum cDNA clone:ddv57d18, 3' ...  1332   0.0    4
(BJ443276) Dictyostelium discoideum cDNA clone:ddv52o10, 3' ...  1332   0.0    2
(BJ442792) Dictyostelium discoideum cDNA clone:ddv51a02, 3' ...  1332   0.0    2
dna update 2008.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   417   e-125
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   150   2e-38
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   139   2e-32
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   139   2e-31
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   135   3e-30
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   129   4e-29
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   120   2e-28
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...   118   8e-28
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   122   2e-27
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...   119   3e-27
protein update 2009. 7.20
PSORT 

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.31  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.83  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHP223 is cyt
5' end seq. ID VHP223F
5' end seq. 
>VHP223F.Seq
TCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTATTA
AAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATGGAA
GTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCACGTA
CTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAACAAT
TAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAATGT
CAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACATTAA
CATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTTATT
ATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATCATA
TTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAGNNN
NNNNNNN
Length of 5' end seq. 547
3' end seq. ID VHP223Z
3' end seq. 
>VHP223Z.Seq
NNNNNNNNNNCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTT
GTAATTGGTGAACTTCCAGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGA
TCCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAAT
TTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGT
TTTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGG
TAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGG
TGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGG
TGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACC
AATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGT
CACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGA
TCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCT
TATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTAT
TGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA
Length of 3' end seq. 766
Connected seq. ID VHP223P
Connected seq. 
>VHP223P.Seq
TCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAACTATTA
AAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACATATGGAA
GTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAACCACGTA
CTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGAAACAAT
TAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATTTAATGT
CAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAACATTAA
CATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAACTTATT
ATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTCATCATA
TTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCGAAG---
-------CAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTTGTA
ATTGGTGAACTTCCAGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGATCC
AAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTT
GGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTT
AATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAA
TGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGT
TCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGA
TGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAAT
CTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCAC
TGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCT
TTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTAT
GCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGG
TGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTA
Length of connected seq. 1293
Full length Seq ID -
Full length Seq. -
Length of full length seq. -