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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP177
Library VH
(Link to library)
Clone ID VHP177
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHP177P
(Link to Original site)
Representative DNA sequence 
>VHP177 (VHP177Q) /CSM/VH/VHP1-D/VHP177Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTXXXXXXXXXXTGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAA
CTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTTGAGTTTTCAAAAATAGGAATCAAATCTAAAAAAAATTTTAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVT---

---NEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVL
YAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIVIGEERRIGSPLFSVKAHLP
VLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRXRKGLAPEIPDL
DKFHEKL*ti*val*vfknrnqi*kkf*k
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvms---

---*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvy
smlln*lppqlx*nqxt**kllpqktllvvftlysivvxvlllvknvvsvphyslskliy
qy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*ppexekv*pqksqi
ltnstknskqfkshfefskigikskknfkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*ch---

---e*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssct
lcc*inclpnxxrtnxlsrnycprkrywwyllctqssxrycyw*rtsyrfpiilcqssft
sirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkxkrfsprnprs
*qiprktlnnlsrtlsfqk*esnlkkilk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVT---

---NEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVL
YAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIVIGEERRIGSPLFSVKAHLP
VLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRXRKGLAPEIPDL
DKFHEKL*ti*val*vfknrnqi*kkf*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1207   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1207   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1207   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1207   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1207   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1207   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1207   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1207   0.0
VHQ435 (VHQ435Q) /CSM/VH/VHQ4-B/VHQ435Q.Seq.d/                       1207   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1207   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1207   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1207   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1207   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1207   0.0    1
(BJ427858) Dictyostelium discoideum cDNA clone:ddv63h20, 5' ...  1207   0.0    1
(BJ427832) Dictyostelium discoideum cDNA clone:ddv63b20, 5' ...  1207   0.0    1
(BJ427795) Dictyostelium discoideum cDNA clone:ddv63j18, 5' ...  1207   0.0    1
(BJ427794) Dictyostelium discoideum cDNA clone:ddv63j17, 5' ...  1207   0.0    1
(BJ427792) Dictyostelium discoideum cDNA clone:ddv63j14, 5' ...  1207   0.0    1
dna update 2008.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   271   3e-71
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   263   8e-69
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   263   1e-68
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   262   2e-68
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   260   6e-68
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   260   6e-68
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   260   8e-68
CU638744_366(CU638744|pid:none) Podospora anserina genomic DNA c...   260   8e-68
AY144924_1(AY144924|pid:none) Saccharomyces castellii clone Cont...   259   1e-67
protein update 2009. 7.20
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHP177 is nuc
5' end seq. ID VHP177F
5' end seq. 
>VHP177F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTNNNNNNNNNN
Length of 5' end seq. 639
3' end seq. ID VHP177Z
3' end seq. 
>VHP177Z.Seq
NNNNNNNNNNTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAA
GAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACC
CTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTA
CTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAA
ATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGT
ATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTA
CCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCT
TTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAA
GCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGAT
CTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAAT
AGGAATCAAATCTAAAAAAAATTTTAAAAAA
Length of 3' end seq. 631
Connected seq. ID VHP177P
Connected seq. 
>VHP177P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACT----------TGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAA
CTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTTGAGTTTTCAAAAATAGGAATCAAATCTAAAAAAAATTTTAAAAAA
Length of connected seq. 1250
Full length Seq ID -
Full length Seq. -
Length of full length seq. -