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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP164
Library VH
(Link to library)
Clone ID VHP164
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13790-1|Contig-U16499-1
Original site URL
Representative seq. ID VHP164P
(Link to Original site)
Representative DNA sequence 
>VHP164 (VHP164Q) /CSM/VH/VHP1-C/VHP164Q.Seq.d/
ATACACTCTCAACCTATTCAAATATAATATGACTGCAACAGATGGTACAAAGGTTATTCA
TGATGATCCATGGTTGGAACCATATAAAGAAGTAATTAAAAGAAGACATAATCAAGTTAA
AAATACCATTCAAAAATTGGAAGAAAGTGAAGGTAGTTTATTAAAATTTTCTCAAGGATA
TGAATACTTTGGTTTCAATGTTACAAAAGATGGTGTAAACTATAGAGAATGGTTACCATC
TGCTCATGAAGTTTATTTAGTTGGTGATTTCAATCAATGGAATAAAACAAGTCATCCACT
TGAAAGAGATAATTATGGTAGATGGAGTATTTTCATTCCAAATAATTCAAATGGTGAATG
TGCAATTCCACATGGTTCAAAAATTAAAATTTATTTAAAATTAGCAAATGGTAATTTTGA
TTATAGAATACCAGCATGGATTAAAAGAGTTGAACAAACCAAAGAGAATCCAGTATTTGA
TGGTGTATTTTGGAATCCAAGTAAACAATATGTATTTAAGAATAAATCACCAATGAAACC
AACAGAGTTGAGAATTTATGAAGCACATGTTGGTATGTCAAGTGAATTACCAGAGATATC
AACATACAGTAAATTCAAGGATACTGTATTACCAATGGXXXXXXXXXXAGGAGAAAAAAG
ATGAAGATTGGAATATGGGTACTATCGCTCACATGCTATCGAATCGTCGTTACAAGGAGA
AGAATATAGCATACGCCGAATCACATGATCAATCATTGGTGGGTGATAAAACTTTGGCAT
TCTGGTTAATGGATAAGGAAATGTATACAAATATGTCTGTCACAACCGAAGAGACTCCAA
TCATTGATCGTGGTATGTCTTTACATAAGATGATTCGTTTGATCACTTCTTCATTGGGTG
GTGATGGTTATCTAAATTTTATGGGTAACGAGTTTGGTCACCCAGAATGGGTGGATTTCC
CACGTGAAGGTAATAACAATAGTTTACATCATGCTCGTCGTAGATGGGACCTCTATAGAA
ATCCATTATTAAGATATAAACAATTACGTGACTTTGATATTGCAATGAATAAAGCTGAAC
AAGAATTCCGTTGGTTATCTTCCGATTTCGCTTATATCTCTTTGAAACATGAAGATGATA
AAATCATAGTTTTCGAACGTGCTTCTCTTATCTTTATCTTTAACTTTCATCCATCTAAAA
GTTTCTCTGATTATAGAATTGGTTCTGGTGTACCAGGTAAATTTATAAATGTTTTAGATT
CNTGATCGTAAAGAATTTGGTGGTCATGTTCGTATTGGTAAAGATAATTATCATTATACT
GAAGATAAACCATGGCATGATAGAAAATATTCTTTATTAATTTATATTCCATCAAGAACT
TGTTTAGTTTTAAAAAAAGTGATTAAAATAATAATAATAATAAAATAAAATAAAATAAAA
AAATAAAAAATAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
YTLNLFKYNMTATDGTKVIHDDPWLEPYKEVIKRRHNQVKNTIQKLEESEGSLLKFSQGY
EYFGFNVTKDGVNYREWLPSAHEVYLVGDFNQWNKTSHPLERDNYGRWSIFIPNNSNGEC
AIPHGSKIKIYLKLANGNFDYRIPAWIKRVEQTKENPVFDGVFWNPSKQYVFKNKSPMKP
TELRIYEAHVGMSSELPEISTYSKFKDTVLPM---

---EKKDEDWNMGTIAHMLSNRRYKEKNIAYAESHDQSLVGDKTLAFWLMDKEMYTNMSV
TTEETPIIDRGMSLHKMIRLITSSLGGDGYLNFMGNEFGHPEWVDFPREGNNNSLHHARR
RWDLYRNPLLRYKQLRDFDIAMNKAEQEFRWLSSDFAYISLKHEDDKIIVFERASLIFIF
NFHPSKSFSDYRIGSGVPGKFINVLDS*s*riwwscsyw*r*lsly*r*tma**kiffin
lysiknlfsfkksd*nnnnnkik*nkkiknk
Translated Amino Acid sequence (All Frames) 
Frame A:
ihsqpiqi*ydcnrwykgys**smvgti*rsn*kkt*ss*kyhskigrk*r*fikifsri
*ilwfqcykrwckl*rmvtics*slfsw*fqsme*nksst*kr*lw*meyfhsk*fkw*m
cnstwfkn*nlfkiskw*f*l*ntsmd*ks*tnqressi*wcilesk*tici*e*itnet
nrvenl*stcwyvk*itrdiniq*iqgycitn---

---rrkkmkigiwvlsltcyrivvtrrri*htpnhminhwwviklwhsg*wirkciqicl
sqpkrlqslivvclyir*fv*sllhwvvmvi*ilwvtslvtqngwishvkvitivyimlv
vdgtsieihy*dinnyvtlilq*iklnknsvgylpislisl*nmkmiks*fsnvlllsls
ltfihlkvsliielvlvyqvnl*mf*ixdrkefgghvrigkdnyhytedkpwhdrkysll
iyipsrtclvlkkvikiiiiik*nkikk*kik

Frame B:
YTLNLFKYNMTATDGTKVIHDDPWLEPYKEVIKRRHNQVKNTIQKLEESEGSLLKFSQGY
EYFGFNVTKDGVNYREWLPSAHEVYLVGDFNQWNKTSHPLERDNYGRWSIFIPNNSNGEC
AIPHGSKIKIYLKLANGNFDYRIPAWIKRVEQTKENPVFDGVFWNPSKQYVFKNKSPMKP
TELRIYEAHVGMSSELPEISTYSKFKDTVLPM---

---gekr*rleygyyrshaiesslqgeeysirrit*siigg**nfgilvng*gnvykyvc
hnrrdsnh*swyvft*ddsfdhffigw*wlskfyg*rvwsprmggfpt*r**q*ftscss
*mgpl*ksiiki*tit*l*ycne*s*triplvifrfrlylfet*r**nhsfrtcfsylyl
*lssi*kfl*l*nwfwctr*iykcfrfxivknlvvmfvlvkiiiiilkinhgmienily*
fifhqelv*f*kk*lk*****nkik*knkk*

Frame C:
tlstysnii*lqqmvqrlfmmihgwnhikk*lkediiklkipfknwkkvkvvy*nflkdm
ntlvsmlqkmv*tiengyhllmkfi*lvisingikqvihlkeiimvdgvfsfqiiqmvnv
qfhmvqklkfi*n*qmviliieyqhglkelnkpkriqylmvyfgiqvnnmylrinhq*nq
qs*efmkhmlvcqvnyqryqhtvnsrilyyqw---

---EKKDEDWNMGTIAHMLSNRRYKEKNIAYAESHDQSLVGDKTLAFWLMDKEMYTNMSV
TTEETPIIDRGMSLHKMIRLITSSLGGDGYLNFMGNEFGHPEWVDFPREGNNNSLHHARR
RWDLYRNPLLRYKQLRDFDIAMNKAEQEFRWLSSDFAYISLKHEDDKIIVFERASLIFIF
NFHPSKSFSDYRIGSGVPGKFINVLDS*s*riwwscsyw*r*lsly*r*tma**kiffin
lysiknlfsfkksd*nnnnnkik*nkkiknk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP164 (VHP164Q) /CSM/VH/VHP1-C/VHP164Q.Seq.d/                       2688   0.0
VHP372 (VHP372Q) /CSM/VH/VHP3-C/VHP372Q.Seq.d/                       1453   0.0
VHK666 (VHK666Q) /CSM/VH/VHK6-C/VHK666Q.Seq.d/                       1453   0.0
VHB511 (VHB511Q) /CSM/VH/VHB5-A/VHB511Q.Seq.d/                       1453   0.0
AHN864 (AHN864Q) /CSM/AH/AHN8-C/AHN864Q.Seq.d/                       1449   0.0
SSB678 (SSB678Q) /CSM/SS/SSB6-D/SSB678Q.Seq.d/                       1445   0.0
VHA456 (VHA456Q) /CSM/VH/VHA4-C/VHA456Q.Seq.d/                       1443   0.0
CHG718 (CHG718Q) /CSM/CH/CHG7-A/CHG718Q.Seq.d/                       1443   0.0
VHB401 (VHB401Q) /CSM/VH/VHB4-A/VHB401Q.Seq.d/                       1441   0.0
VHB844 (VHB844Q) /CSM/VH/VHB8-B/VHB844Q.Seq.d/                       1437   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446114) Dictyostelium discoideum cDNA clone:ddv61o15, 3' ...  1465   0.0    1
(BJ427220) Dictyostelium discoideum cDNA clone:ddv61o15, 5' ...  1261   0.0    1
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...  1215   0.0    2
(BJ446120) Dictyostelium discoideum cDNA clone:ddv61p17, 3' ...  1215   0.0    2
(BJ443301) Dictyostelium discoideum cDNA clone:ddv52c18, 3' ...  1215   0.0    2
(BJ437368) Dictyostelium discoideum cDNA clone:ddv34e03, 3' ...  1215   0.0    2
(BJ379492) Dictyostelium discoideum cDNA clone:ddc35d05, 3' ...  1215   0.0    2
(BJ355940) Dictyostelium discoideum cDNA clone:dda58p16, 3' ...  1211   0.0    2
(BJ443594) Dictyostelium discoideum cDNA clone:ddv53o08, 3' ...  1207   0.0    4
(BJ438876) Dictyostelium discoideum cDNA clone:ddv38b21, 3' ...  1207   0.0    2
dna update 2008.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_3(AC116986|pid:none) Dictyostelium discoideum chromosom...   431   e-140
(Q555Q9) RecName: Full=1,4-alpha-glucan-branching enzyme;       ...   431   e-140
BC076746_1(BC076746|pid:none) Xenopus laevis glucan (1,4-alpha-)...   292   4e-86
AB209731_1(AB209731|pid:none) Homo sapiens mRNA for Glucan , bra...   301   7e-86
(Q04446) RecName: Full=1,4-alpha-glucan-branching enzyme;       ...   301   7e-86
A46075(A46075) 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) -...   301   7e-86
AK300620_1(AK300620|pid:none) Homo sapiens cDNA FLJ59760 complet...   301   7e-86
AK297939_1(AK297939|pid:none) Homo sapiens cDNA FLJ57129 complet...   301   7e-86
(Q9D6Y9) RecName: Full=1,4-alpha-glucan-branching enzyme;       ...   298   2e-85
EU442575_1(EU442575|pid:none) Bos taurus 1,4-alpha-glucan branch...   303   7e-85
protein update 2009. 7.20
PSORT 

 psg: 0.69  gvh: 0.30  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHP164 is cyt
5' end seq. ID VHP164F
5' end seq. 
>VHP164F.Seq
ATACACTCTCAACCTATTCAAATATAATATGACTGCAACAGATGGTACAAAGGTTATTCA
TGATGATCCATGGTTGGAACCATATAAAGAAGTAATTAAAAGAAGACATAATCAAGTTAA
AAATACCATTCAAAAATTGGAAGAAAGTGAAGGTAGTTTATTAAAATTTTCTCAAGGATA
TGAATACTTTGGTTTCAATGTTACAAAAGATGGTGTAAACTATAGAGAATGGTTACCATC
TGCTCATGAAGTTTATTTAGTTGGTGATTTCAATCAATGGAATAAAACAAGTCATCCACT
TGAAAGAGATAATTATGGTAGATGGAGTATTTTCATTCCAAATAATTCAAATGGTGAATG
TGCAATTCCACATGGTTCAAAAATTAAAATTTATTTAAAATTAGCAAATGGTAATTTTGA
TTATAGAATACCAGCATGGATTAAAAGAGTTGAACAAACCAAAGAGAATCCAGTATTTGA
TGGTGTATTTTGGAATCCAAGTAAACAATATGTATTTAAGAATAAATCACCAATGAAACC
AACAGAGTTGAGAATTTATGAAGCACATGTTGGTATGTCAAGTGAATTACCAGAGATATC
AACATACAGTAAATTCAAGGATACTGTATTACCAATGGNNNNNNNNNN
Length of 5' end seq. 648
3' end seq. ID VHP164Z
3' end seq. 
>VHP164Z.Seq
NNNNNNNNNNAGGAGAAAAAAGATGAAGATTGGAATATGGGTACTATCGCTCACATGCTA
TCGAATCGTCGTTACAAGGAGAAGAATATAGCATACGCCGAATCACATGATCAATCATTG
GTGGGTGATAAAACTTTGGCATTCTGGTTAATGGATAAGGAAATGTATACAAATATGTCT
GTCACAACCGAAGAGACTCCAATCATTGATCGTGGTATGTCTTTACATAAGATGATTCGT
TTGATCACTTCTTCATTGGGTGGTGATGGTTATCTAAATTTTATGGGTAACGAGTTTGGT
CACCCAGAATGGGTGGATTTCCCACGTGAAGGTAATAACAATAGTTTACATCATGCTCGT
CGTAGATGGGACCTCTATAGAAATCCATTATTAAGATATAAACAATTACGTGACTTTGAT
ATTGCAATGAATAAAGCTGAACAAGAATTCCGTTGGTTATCTTCCGATTTCGCTTATATC
TCTTTGAAACATGAAGATGATAAAATCATAGTTTTCGAACGTGCTTCTCTTATCTTTATC
TTTAACTTTCATCCATCTAAAAGTTTCTCTGATTATAGAATTGGTTCTGGTGTACCAGGT
AAATTTATAAATGTTTTAGATTCNTGATCGTAAAGAATTTGGTGGTCATGTTCGTATTGG
TAAAGATAATTATCATTATACTGAAGATAAACCATGGCATGATAGAAAATATTCTTTATT
AATTTATATTCCATCAAGAACTTGTTTAGTTTTAAAAAAAGTGATTAAAATAATAATAAT
AATAAAATAAAATAAAATAAAAAAATAAAAAATAAAA
Length of 3' end seq. 817
Connected seq. ID VHP164P
Connected seq. 
>VHP164P.Seq
ATACACTCTCAACCTATTCAAATATAATATGACTGCAACAGATGGTACAAAGGTTATTCA
TGATGATCCATGGTTGGAACCATATAAAGAAGTAATTAAAAGAAGACATAATCAAGTTAA
AAATACCATTCAAAAATTGGAAGAAAGTGAAGGTAGTTTATTAAAATTTTCTCAAGGATA
TGAATACTTTGGTTTCAATGTTACAAAAGATGGTGTAAACTATAGAGAATGGTTACCATC
TGCTCATGAAGTTTATTTAGTTGGTGATTTCAATCAATGGAATAAAACAAGTCATCCACT
TGAAAGAGATAATTATGGTAGATGGAGTATTTTCATTCCAAATAATTCAAATGGTGAATG
TGCAATTCCACATGGTTCAAAAATTAAAATTTATTTAAAATTAGCAAATGGTAATTTTGA
TTATAGAATACCAGCATGGATTAAAAGAGTTGAACAAACCAAAGAGAATCCAGTATTTGA
TGGTGTATTTTGGAATCCAAGTAAACAATATGTATTTAAGAATAAATCACCAATGAAACC
AACAGAGTTGAGAATTTATGAAGCACATGTTGGTATGTCAAGTGAATTACCAGAGATATC
AACATACAGTAAATTCAAGGATACTGTATTACCAATGG----------AGGAGAAAAAAG
ATGAAGATTGGAATATGGGTACTATCGCTCACATGCTATCGAATCGTCGTTACAAGGAGA
AGAATATAGCATACGCCGAATCACATGATCAATCATTGGTGGGTGATAAAACTTTGGCAT
TCTGGTTAATGGATAAGGAAATGTATACAAATATGTCTGTCACAACCGAAGAGACTCCAA
TCATTGATCGTGGTATGTCTTTACATAAGATGATTCGTTTGATCACTTCTTCATTGGGTG
GTGATGGTTATCTAAATTTTATGGGTAACGAGTTTGGTCACCCAGAATGGGTGGATTTCC
CACGTGAAGGTAATAACAATAGTTTACATCATGCTCGTCGTAGATGGGACCTCTATAGAA
ATCCATTATTAAGATATAAACAATTACGTGACTTTGATATTGCAATGAATAAAGCTGAAC
AAGAATTCCGTTGGTTATCTTCCGATTTCGCTTATATCTCTTTGAAACATGAAGATGATA
AAATCATAGTTTTCGAACGTGCTTCTCTTATCTTTATCTTTAACTTTCATCCATCTAAAA
GTTTCTCTGATTATAGAATTGGTTCTGGTGTACCAGGTAAATTTATAAATGTTTTAGATT
CNTGATCGTAAAGAATTTGGTGGTCATGTTCGTATTGGTAAAGATAATTATCATTATACT
GAAGATAAACCATGGCATGATAGAAAATATTCTTTATTAATTTATATTCCATCAAGAACT
TGTTTAGTTTTAAAAAAAGTGATTAAAATAATAATAATAATAAAATAAAATAAAATAAAA
AAATAAAAAATAAAA
Length of connected seq. 1445
Full length Seq ID -
Full length Seq. -
Length of full length seq. -