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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP139
Library VH
(Link to library)
Clone ID VHP139
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHP139P
(Link to Original site)
Representative DNA sequence 
>VHP139 (VHP139Q) /CSM/VH/VHP1-B/VHP139Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAXXXXXXXXXXTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCA
AAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCA
CCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATG
TCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTC
GTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTA
NAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTC
ATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCA
AAAATAGGAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknrnqi*kkf*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlki---

---srr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpp
h*chpqrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkrywwyllctqssxryc
yw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmcxrslgfnwccqqr*esh
*srfshqkxkrfsprnprs*qiprktlnnlsrtlsfqk*esnlkkilkkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknrnqi*kkf*kk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqlx*nqxt**kllpqktllvvftlysivvxvll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvxsiiglqlvlstkirkpl
ksl*ppexekv*pqksqiltnstknskqfkshfefskigikskknfkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP139 (VHP139Q) /CSM/VH/VHP1-B/VHP139Q.Seq.d/                       2399   0.0
VHE364 (VHE364Q) /CSM/VH/VHE3-C/VHE364Q.Seq.d/                       1271   0.0
VHB458 (VHB458Q) /CSM/VH/VHB4-C/VHB458Q.Seq.d/                       1271   0.0
VHB371 (VHB371Q) /CSM/VH/VHB3-C/VHB371Q.Seq.d/                       1271   0.0
VHA230 (VHA230Q) /CSM/VH/VHA2-B/VHA230Q.Seq.d/                       1271   0.0
VHP196 (VHP196Q) /CSM/VH/VHP1-D/VHP196Q.Seq.d/                       1269   0.0
VHP776 (VHP776Q) /CSM/VH/VHP7-D/VHP776Q.Seq.d/                       1265   0.0
VHP166 (VHP166Q) /CSM/VH/VHP1-C/VHP166Q.Seq.d/                       1265   0.0
VHB347 (VHB347Q) /CSM/VH/VHB3-B/VHB347Q.Seq.d/                       1265   0.0
VHA658 (VHA658Q) /CSM/VH/VHA6-C/VHA658Q.Seq.d/                       1265   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446029) Dictyostelium discoideum cDNA clone:ddv61m09, 3' ...  1271   0.0    1
(BJ439195) Dictyostelium discoideum cDNA clone:ddv39p15, 3' ...  1271   0.0    1
(BJ437258) Dictyostelium discoideum cDNA clone:ddv33n17, 3' ...  1271   0.0    1
(BJ437205) Dictyostelium discoideum cDNA clone:ddv33d16, 3' ...  1271   0.0    1
(BJ436506) Dictyostelium discoideum cDNA clone:ddv31k08, 3' ...  1271   0.0    1
(BJ446191) Dictyostelium discoideum cDNA clone:ddv61o23, 3' ...  1269   0.0    1
(BJ446473) Dictyostelium discoideum cDNA clone:ddv62h19, 3' ...  1265   0.0    1
(BJ446062) Dictyostelium discoideum cDNA clone:ddv61c17, 3' ...  1265   0.0    1
(BJ445863) Dictyostelium discoideum cDNA clone:ddv60j23, 3' ...  1265   0.0    1
(BJ445738) Dictyostelium discoideum cDNA clone:ddv60a17, 3' ...  1265   0.0    1
dna update 2008.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   261   4e-68
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   260   9e-68
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   259   1e-67
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   259   2e-67
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   258   3e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   257   6e-67
protein update 2009. 7.20
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHP139 is nuc
5' end seq. ID VHP139F
5' end seq. 
>VHP139F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATANNNNNNNNNN
Length of 5' end seq. 619
3' end seq. ID VHP139Z
3' end seq. 
>VHP139Z.Seq
NNNNNNNNNNTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTC
AATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAG
GTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCC
ACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCT
ATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTA
CTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTG
TTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAG
TATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCC
CACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCA
CTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTG
ACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGGA
ATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of 3' end seq. 691
Connected seq. ID VHP139P
Connected seq. 
>VHP139P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATA----------TCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCA
AAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCA
CCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATG
TCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTC
GTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTA
NAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTC
ATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCA
AAAATAGGAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of connected seq. 1290
Full length Seq ID -
Full length Seq. -
Length of full length seq. -