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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP132
Library VH
(Link to library)
Clone ID VHP132
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHP132P
(Link to Original site)
Representative DNA sequence 
>VHP132 (VHP132Q) /CSM/VH/VHP1-B/VHP132Q.Seq.d/
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCANAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCNAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACNAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAAAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCNAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACAXX
XXXXXXXXCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATNTAAGTCGC
sequence update 2002.10.25
Translated Amino Acid sequence 
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIXNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFXMPKEDKLPAGCTSHXFLINLIDSPGHVDF
SSKVTAALRVTDGALVVIDCVXGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLXLQLN-
--

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*tx*v
Translated Amino Acid sequence (All Frames) 
Frame A:
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIXNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFXMPKEDKLPAGCTSHXFLINLIDSPGHVDF
SSKVTAALRVTDGALVVIDCVXGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLXLQLN-
--

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*tx*v

Frame B:
tleckkk*stkwlisqsikfvplwtvvkisxicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsyxnsn*t-
--

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivvevll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqxks

Frame C:
lssakkskvqng*fhnrsnschygps*kyxkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslxnakgrqitswlyftxifnqsyrfprsr*fq
fksnccppcy*wcfsryrlrxrclrtnrncitssrr*tyqtslirqqsrsflixtpik--
-

---rr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss*rycy
w*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkekrfsprnprs*qiprktlnnxsr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1235   0.0
VHQ483 (VHQ483Q) /CSM/VH/VHQ4-D/VHQ483Q.Seq.d/                       1235   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1235   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1235   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1235   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1235   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1235   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1235   0.0
VHQ372 (VHQ372Q) /CSM/VH/VHQ3-C/VHQ372Q.Seq.d/                       1235   0.0
VHQ359 (VHQ359Q) /CSM/VH/VHQ3-C/VHQ359Q.Seq.d/                       1235   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU039543) Dictyostelium discoideum slug cDNA, clone SLE722.     1235   0.0    1
(AU039435) Dictyostelium discoideum slug cDNA, clone SLH782.     1235   0.0    1
(AU034446) Dictyostelium discoideum slug cDNA, clone SLC886.     1235   0.0    1
(AU033620) Dictyostelium discoideum slug cDNA, clone SLB237.     1235   0.0    1
(BJ446824) Dictyostelium discoideum cDNA clone:ddv63o22, 3' ...  1235   0.0    1
(BJ446821) Dictyostelium discoideum cDNA clone:ddv63o19, 3' ...  1235   0.0    1
(BJ446813) Dictyostelium discoideum cDNA clone:ddv63m22, 3' ...  1235   0.0    1
(BJ446806) Dictyostelium discoideum cDNA clone:ddv63k22, 3' ...  1235   0.0    1
(BJ446805) Dictyostelium discoideum cDNA clone:ddv63k21, 3' ...  1235   0.0    1
(BJ446797) Dictyostelium discoideum cDNA clone:ddv63i24, 3' ...  1235   0.0    1
dna update 2008.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   267   5e-70
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   265   2e-69
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   265   3e-69
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   263   7e-69
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   263   9e-69
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   263   1e-68
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   263   1e-68
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   262   2e-68
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   261   3e-68
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   261   3e-68
protein update 2009. 7.20
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.31  top: 0.47  tms: 0.07  mit: 0.38  mip: 0.02
 nuc: 0.04  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 24.0 %: nuclear
	 12.0 %: Golgi
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  4.0 %: vacuolar

>> prediction for VHP132 is mit
5' end seq. ID VHP132F
5' end seq. 
>VHP132F.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCANAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCNAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACNAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAAAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCNAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACANN
NNNNNNNN
Length of 5' end seq. 548
3' end seq. ID VHP132Z
3' end seq. 
>VHP132Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTA
TGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATNTAAGTCGC
Length of 3' end seq. 636
Connected seq. ID VHP132P
Connected seq. 
>VHP132P.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCANAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCNAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACNAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAAAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCNAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACA--
--------CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAAT
ATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTG
TTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACA
CTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATG
CTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTAT
TAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTG
AAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATNTAAGTCGC
Length of connected seq. 1164
Full length Seq ID -
Full length Seq. -
Length of full length seq. -