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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP105
Library VH
(Link to library)
Clone ID VHP105
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15792-1
Original site URL
Representative seq. ID VHP105P
(Link to Original site)
Representative DNA sequence 
>VHP105 (VHP105Q) /CSM/VH/VHP1-A/VHP105Q.Seq.d/
ACTCAACAGCCTGCCTCCGTTTTGTGTTTGGTTCTTTTATACCAAATAAACAAGTTTTAT
GGCAAGAAGCTCATAATAAACATACATGTGCCTTTAATCAATCTGTAATCTGTTCTATTA
CACGATTTTCATGCAGTCACAACAACTACCCAAAAATGTTTAGTTTAGGAAAATTGGTTA
AAAAAGATGCTTCTTTTTATAGATATATAACAAATGTTAATAAAGATTTAAAAATTAAAC
CAATTACAAAGATATTAATTGCAAATAGAGGTGAAATTGCATGTCGTGTAATGAGAACAG
CAAAATCAAAAGGTGTAAAAACCGTAGCAGTTTATAGTGAAGCAGATAAGAATTCATTAC
ATGTTTCAATGGCAGATGAGAGTTATTTAATTGGACCAGCAGCAGCCAAAGAGAGTTATT
TATGTGGAAATAAGATCATAGATGTAGCAAAGAGATCTGGAGCACAAGCAATTCATCCAG
GTTATGGTTTCTTATCAGAGAATTCAGATTTTGCTGATCTCTGTGAGAGAGAAGGTATCA
TTTTCATTGGACCACCATCAGATGCAATCAAAGCAATGGGTAGCAAAAGTGCCTCAAAGG
ATATTATGATCAAAGCTGGCGTACCAACCATCCXXXXXXXXXXCCACAAGCTCCAATGTC
TGATGATTCATTAGCATTGGCTGCCACAAGTTTACTCTTAAAAGAGATCACTCAACAAAA
ATCAAAAGAAGATCCAAACTCACCTTGGTCAAGTTTAGGTGGTTTCCGTATTAATCATAA
TTTAAAAAAACAAGTTAAATTCAATCAAAAAGATAATAAAGTTGTTGTTAATGTTGAATT
CATTGGTGGTGGTGGTGCTGCTGCTAATGGTAAACATAACTTTAAAGTAACTTTAGATAA
TGGTAATGTCGTTGAAGTTTTAGATGCAAAATTAAATCAAAATAATGAAACTATTAGTGC
TCATGTAAATGGTAGATTCTATAATAACATTAAATCCGTCATTGTAAAGGATACTTTAAC
AATCTTTAATGAAGGTCAACAATACCAATTGGATATTCCTCAAGATGTTAAACCAAAAGG
TGCTGATGGTGTATTGGGTTCTTTAGTTTCACCAATGCCTGGAAAAATCACTAAAGTTAT
GGTAAATGTTGGTGACATGGTTAAAAAGGGTCAACCAATCTTACTCATGGAAGCAATGAA
AATGGAACATACTATTCGTTCTCCAATCGATGGTAAAGTTGAATCATTACCTTATAATGT
TAAGAAATCGTTGAGGATAAGAAAACTT
sequence update 2002.10.25
Translated Amino Acid sequence 
STACLRFVFGSFIPNKQVLWQEAHNKHTCAFNQSVICSITRFSCSHNNYPKMFSLGKLVK
KDASFYRYITNVNKDLKIKPITKILIANRGEIACRVMRTAKSKGVKTVAVYSEADKNSLH
VSMADESYLIGPAAAKESYLCGNKIIDVAKRSGAQAIHPGYGFLSENSDFADLCEREGII
FIGPPSDAIKAMGSKSASKDIMIKAGVPTI---

---PQAPMSDDSLALAATSLLLKEITQQKSKEDPNSPWSSLGGFRINHNLKKQVKFNQKD
NKVVVNVEFIGGGGAAANGKHNFKVTLDNGNVVEVLDAKLNQNNETISAHVNGRFYNNIK
SVIVKDTLTIFNEGQQYQLDIPQDVKPKGADGVLGSLVSPMPGKITKVMVNVGDMVKKGQ
PILLMEAMKMEHTIRSPIDGKVESLPYNVKKSLRIRKL
Translated Amino Acid sequence (All Frames) 
Frame A:
tqqpasvlclvllyqinkfygkkliinihvplinl*svllhdfhavttttqkclv*enwl
kkmllfidi*qmliki*klnqlqry*lqievklhvv**eqqnqkv*kp*qfivkqirihy
mfqwqmrvi*ldqqqpkrviyveirs*m*qrdlehkqfiqvmvsyqriqillisvrekvs
fsldhhqmqskqwvakvpqril*sklayqps---

---PQAPMSDDSLALAATSLLLKEITQQKSKEDPNSPWSSLGGFRINHNLKKQVKFNQKD
NKVVVNVEFIGGGGAAANGKHNFKVTLDNGNVVEVLDAKLNQNNETISAHVNGRFYNNIK
SVIVKDTLTIFNEGQQYQLDIPQDVKPKGADGVLGSLVSPMPGKITKVMVNVGDMVKKGQ
PILLMEAMKMEHTIRSPIDGKVESLPYNVKKSLRIRKL

Frame B:
lnslppfcvwffytk*tsfmarss**tymcl*sicnlfyytifmqsqqlpknv*frkig*
krcffl*iynkc**rfkn*tnykdinck*r*ncmscnenskikrcknrssl**sr*efit
cfngr*elfnwtsssqrelfmwk*dhrcskeiwstsnssrlwflirefrfc*sl*erryh
fhwttircnqsng*qkclkgyydqswrtnh---

---hklqclmih*hwlpqvys*krslnknqkkiqthlgqv*vvsvliii*knklnsikki
iklllmlnslvvvvlllmvnitlk*l*imvmslkf*mqn*ikimkllvlm*mvdsiitln
psl*ril*qslmkvnntnwiflkmlnqkvlmvywvl*fhqclekslklw*mlvtwlkrvn
qsyswkq*kwnilfvlqsmvklnhylimlrnr*g*en

Frame C:
STACLRFVFGSFIPNKQVLWQEAHNKHTCAFNQSVICSITRFSCSHNNYPKMFSLGKLVK
KDASFYRYITNVNKDLKIKPITKILIANRGEIACRVMRTAKSKGVKTVAVYSEADKNSLH
VSMADESYLIGPAAAKESYLCGNKIIDVAKRSGAQAIHPGYGFLSENSDFADLCEREGII
FIGPPSDAIKAMGSKSASKDIMIKAGVPTI---

---tssnv**fisigchkftlkrdhstkikrrskltlvkfrwfpy*s*fkkts*iqskr*
*scc*c*ihwwwwccc*w*t*l*snfr*w*cr*sfrckiksk**ny*csckw*il**h*i
rhckgyfnnl**rstipigyssrc*tkrc*wcigffsftnawknh*sygkcw*hg*kgst
nlthgsnengtyysfsnrw*s*iitl*c*eivedkkt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP105 (VHP105Q) /CSM/VH/VHP1-A/VHP105Q.Seq.d/                       2452   0.0
VHJ432 (VHJ432Q) /CSM/VH/VHJ4-B/VHJ432Q.Seq.d/                       1229   0.0
VHK848 (VHK848Q) /CSM/VH/VHK8-B/VHK848Q.Seq.d/                       1223   0.0
VHD217 (VHD217Q) /CSM/VH/VHD2-A/VHD217Q.Seq.d/                       1223   0.0
SFI331 (SFI331Q) /CSM/SF/SFI3-B/SFI331Q.Seq.d/                       1207   0.0
VHP809 (VHP809Q) /CSM/VH/VHP8-A/VHP809Q.Seq.d/                       1199   0.0
VHK824 (VHK824Q) /CSM/VH/VHK8-A/VHK824Q.Seq.d/                       1197   0.0
VHE237 (VHE237Q) /CSM/VH/VHE2-B/VHE237Q.Seq.d/                       1197   0.0
VHC101 (VHC101Q) /CSM/VH/VHC1-A/VHC101Q.Seq.d/                       1158   0.0
VFA789 (VFA789Q) /CSM/VF/VFA7-D/VFA789Q.Seq.d/                       1150   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427045) Dictyostelium discoideum cDNA clone:ddv61i01, 5' ...  1255   0.0    1
(BJ445930) Dictyostelium discoideum cDNA clone:ddv61i01, 3' ...   987   0.0    2
(BJ442303) Dictyostelium discoideum cDNA clone:ddv49p08, 3' ...   987   0.0    2
(BJ437665) Dictyostelium discoideum cDNA clone:ddv35a01, 3' ...   987   0.0    2
(BJ440914) Dictyostelium discoideum cDNA clone:ddv45i05, 3' ...   981   0.0    2
(BJ440542) Dictyostelium discoideum cDNA clone:ddv44c02, 3' ...   981   0.0    2
(BJ446207) Dictyostelium discoideum cDNA clone:ddv62b04, 3' ...   948   0.0    2
(BJ402590) Dictyostelium discoideum cDNA clone:dds17n07, 3' ...   948   0.0    3
(BJ443284) Dictyostelium discoideum cDNA clone:ddv52p12, 3' ...   938   0.0    3
(BJ438454) Dictyostelium discoideum cDNA clone:ddv37a06, 3' ...   938   0.0    3
dna update 2008.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KE6) RecName: Full=Methylcrotonoyl-CoA carboxylase subunit a...   362   2e-98
BC021382_1(BC021382|pid:none) Mus musculus methylcrotonoyl-Coenz...   192   2e-47
(Q99MR8) RecName: Full=Methylcrotonoyl-CoA carboxylase subunit a...   192   2e-47
AJ720239_1(AJ720239|pid:none) Gallus gallus mRNA for hypothetica...   191   4e-47
BC125958_1(BC125958|pid:none) Danio rerio zgc:154106, mRNA (cDNA...   191   4e-47
BX005105_1(BX005105|pid:none) Zebrafish DNA sequence from clone ...   191   4e-47
AE014297_5001(AE014297|pid:none) Drosophila melanogaster chromos...   189   2e-46
(Q96RQ3) RecName: Full=Methylcrotonoyl-CoA carboxylase subunit a...   188   3e-46
AF310339_1(AF310339|pid:none) Homo sapiens 3-methylcrotonyl-CoA ...   188   3e-46
(Q5I0C3) RecName: Full=Methylcrotonoyl-CoA carboxylase subunit a...   188   3e-46
protein update 2009. 7.20
PSORT 

 psg: 0.94  gvh: 0.39  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.16
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHP105 is mit
5' end seq. ID VHP105F
5' end seq. 
>VHP105F.Seq
ACTCAACAGCCTGCCTCCGTTTTGTGTTTGGTTCTTTTATACCAAATAAACAAGTTTTAT
GGCAAGAAGCTCATAATAAACATACATGTGCCTTTAATCAATCTGTAATCTGTTCTATTA
CACGATTTTCATGCAGTCACAACAACTACCCAAAAATGTTTAGTTTAGGAAAATTGGTTA
AAAAAGATGCTTCTTTTTATAGATATATAACAAATGTTAATAAAGATTTAAAAATTAAAC
CAATTACAAAGATATTAATTGCAAATAGAGGTGAAATTGCATGTCGTGTAATGAGAACAG
CAAAATCAAAAGGTGTAAAAACCGTAGCAGTTTATAGTGAAGCAGATAAGAATTCATTAC
ATGTTTCAATGGCAGATGAGAGTTATTTAATTGGACCAGCAGCAGCCAAAGAGAGTTATT
TATGTGGAAATAAGATCATAGATGTAGCAAAGAGATCTGGAGCACAAGCAATTCATCCAG
GTTATGGTTTCTTATCAGAGAATTCAGATTTTGCTGATCTCTGTGAGAGAGAAGGTATCA
TTTTCATTGGACCACCATCAGATGCAATCAAAGCAATGGGTAGCAAAAGTGCCTCAAAGG
ATATTATGATCAAAGCTGGCGTACCAACCATCCNNNNNNNNNN
Length of 5' end seq. 643
3' end seq. ID VHP105Z
3' end seq. 
>VHP105Z.Seq
NNNNNNNNNNCCACAAGCTCCAATGTCTGATGATTCATTAGCATTGGCTGCCACAAGTTT
ACTCTTAAAAGAGATCACTCAACAAAAATCAAAAGAAGATCCAAACTCACCTTGGTCAAG
TTTAGGTGGTTTCCGTATTAATCATAATTTAAAAAAACAAGTTAAATTCAATCAAAAAGA
TAATAAAGTTGTTGTTAATGTTGAATTCATTGGTGGTGGTGGTGCTGCTGCTAATGGTAA
ACATAACTTTAAAGTAACTTTAGATAATGGTAATGTCGTTGAAGTTTTAGATGCAAAATT
AAATCAAAATAATGAAACTATTAGTGCTCATGTAAATGGTAGATTCTATAATAACATTAA
ATCCGTCATTGTAAAGGATACTTTAACAATCTTTAATGAAGGTCAACAATACCAATTGGA
TATTCCTCAAGATGTTAAACCAAAAGGTGCTGATGGTGTATTGGGTTCTTTAGTTTCACC
AATGCCTGGAAAAATCACTAAAGTTATGGTAAATGTTGGTGACATGGTTAAAAAGGGTCA
ACCAATCTTACTCATGGAAGCAATGAAAATGGAACATACTATTCGTTCTCCAATCGATGG
TAAAGTTGAATCATTACCTTATAATGTTAAGAAATCGTTGAGGATAAGAAAACTT
Length of 3' end seq. 655
Connected seq. ID VHP105P
Connected seq. 
>VHP105P.Seq
ACTCAACAGCCTGCCTCCGTTTTGTGTTTGGTTCTTTTATACCAAATAAACAAGTTTTAT
GGCAAGAAGCTCATAATAAACATACATGTGCCTTTAATCAATCTGTAATCTGTTCTATTA
CACGATTTTCATGCAGTCACAACAACTACCCAAAAATGTTTAGTTTAGGAAAATTGGTTA
AAAAAGATGCTTCTTTTTATAGATATATAACAAATGTTAATAAAGATTTAAAAATTAAAC
CAATTACAAAGATATTAATTGCAAATAGAGGTGAAATTGCATGTCGTGTAATGAGAACAG
CAAAATCAAAAGGTGTAAAAACCGTAGCAGTTTATAGTGAAGCAGATAAGAATTCATTAC
ATGTTTCAATGGCAGATGAGAGTTATTTAATTGGACCAGCAGCAGCCAAAGAGAGTTATT
TATGTGGAAATAAGATCATAGATGTAGCAAAGAGATCTGGAGCACAAGCAATTCATCCAG
GTTATGGTTTCTTATCAGAGAATTCAGATTTTGCTGATCTCTGTGAGAGAGAAGGTATCA
TTTTCATTGGACCACCATCAGATGCAATCAAAGCAATGGGTAGCAAAAGTGCCTCAAAGG
ATATTATGATCAAAGCTGGCGTACCAACCATCC----------CCACAAGCTCCAATGTC
TGATGATTCATTAGCATTGGCTGCCACAAGTTTACTCTTAAAAGAGATCACTCAACAAAA
ATCAAAAGAAGATCCAAACTCACCTTGGTCAAGTTTAGGTGGTTTCCGTATTAATCATAA
TTTAAAAAAACAAGTTAAATTCAATCAAAAAGATAATAAAGTTGTTGTTAATGTTGAATT
CATTGGTGGTGGTGGTGCTGCTGCTAATGGTAAACATAACTTTAAAGTAACTTTAGATAA
TGGTAATGTCGTTGAAGTTTTAGATGCAAAATTAAATCAAAATAATGAAACTATTAGTGC
TCATGTAAATGGTAGATTCTATAATAACATTAAATCCGTCATTGTAAAGGATACTTTAAC
AATCTTTAATGAAGGTCAACAATACCAATTGGATATTCCTCAAGATGTTAAACCAAAAGG
TGCTGATGGTGTATTGGGTTCTTTAGTTTCACCAATGCCTGGAAAAATCACTAAAGTTAT
GGTAAATGTTGGTGACATGGTTAAAAAGGGTCAACCAATCTTACTCATGGAAGCAATGAA
AATGGAACATACTATTCGTTCTCCAATCGATGGTAAAGTTGAATCATTACCTTATAATGT
TAAGAAATCGTTGAGGATAAGAAAACTT
Length of connected seq. 1278
Full length Seq ID -
Full length Seq. -
Length of full length seq. -