NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO809
Library VH
(Link to library)
Clone ID VHO809
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12842-1
Original site URL
Representative seq. ID VHO809P
(Link to Original site)
Representative DNA sequence 
>VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGACTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATTXXXXXXXXXXTATACTCATAGCT
TTCTTTACACGAGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAAGTTGG
CCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATCAATTT
GTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCTCAACT
CCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTATCGATG
AATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTATCCCAT
ATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTTAATGT
ACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTTAATAA
AAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTTATTAG
GCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAGAATAT
TTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTTTTATA
ATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTACAACCA
GTAGTTACTGTGGGATTAGAAAAAATNATCAATANAAATTCCAGGATTAAAATTAATAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGL
LISNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNS
VYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPK
EYFNGTIIRLP---

---YTHSFLYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLS
DFITSLNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSF
DKSSLLMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSK
EIDKLRIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCGIRKNXQXK
FQD*n**
Translated Amino Acid sequence (All Frames) 
Frame A:
keikcpkikvkq*nylif*eiifltiqnqkylen*fkmqkmleliqs**rlmkahiqimg
c*fqmitkklkvqkny*vhqf*fiimqlfknmtgkefvklvkvvkkki*kvlaisv*vgt
vfii*qiiqlfivvimfgsqiqtke*vvvysli*mtkmiirnvlvf*shsfnlknqivtq
knilmvqsfvyh---

---YTHSFLYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLS
DFITSLNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSF
DKSSLLMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSK
EIDKLRIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCGIRKNXQXK
FQD*n**

Frame B:
kr*napk*ksnnktisffkr*ss*lsrikni*ridskcrrcss*yshnkg**rlisk*wv
vnfk**prn*kfkriirsinfnl**cnfsrt*lgrnlsn*sr**krkfkkcwqfrfrleq
clsynr*sncl*w**clvlrskrknewwfil*fr*rr*l*emf*ffraihsi*rikl*pk
rif*wynhssti---

---iliafftrviksmisysnimviswpni*nflhyh*tldn*fhqfveii*ileklnfq
ilslvstpsttsnf*viv**qfgivsmn*kekkl*nylisnhyklshid*vqlklmslvl
inlhc*ctnqisyqlnqs*tn*ksi**kkdiksivy*memlksqfy*aifkg*iyh*tqr
ksin*eyf*lnqcskflyhkiilqffiiyqfthaleivtkslvvlqpvvtvglekxinxn
srikink

Frame C:
RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGL
LISNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNS
VYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPK
EYFNGTIIRLP---

---ys*lslhe*ssl*sliqilw**vgqtfkifyitikl*tinfinl*klyky*rn*tfr
fyh*sqlhqqppifk**ynnnlelyr*ikkkrnfriisypiitnypi*iksn*n*ch*f*
*ifivnvqikfltn*tnlkqtknlfnkrrtsnq*firwkcsnpnfirlsskgkfiirlkg
nr*iknifs*invpnffitrlyfnfl*ftnspmhwk**pkv**yynq*llwd*kkxsixi
pglkli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                       1891   0.0
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                       1441   0.0
VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                       1427   0.0
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                       1413   0.0
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                       1356   0.0
VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                       1344   0.0
VFN211 (VFN211Q) /CSM/VF/VFN2-A/VFN211Q.Seq.d/                       1176   0.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        609   e-173
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        609   e-173
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        593   e-168
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445598) Dictyostelium discoideum cDNA clone:ddv60b04, 3' ...  1441   0.0    1
(BJ437533) Dictyostelium discoideum cDNA clone:ddv34f17, 3' ...  1441   0.0    1
(BJ439015) Dictyostelium discoideum cDNA clone:ddv39m05, 3' ...  1358   0.0    2
(BJ438600) Dictyostelium discoideum cDNA clone:ddv37m10, 3' ...  1358   0.0    3
(BJ443404) Dictyostelium discoideum cDNA clone:ddv52f23, 3' ...  1328   0.0    2
(BJ443141) Dictyostelium discoideum cDNA clone:ddv52d04, 3' ...  1328   0.0    2
(BJ416709) Dictyostelium discoideum cDNA clone:ddv27e04, 5' ...  1176   0.0    1
(BJ426718) Dictyostelium discoideum cDNA clone:ddv60b04, 5' ...   617   0.0    2
(BJ426269) Dictyostelium discoideum cDNA clone:ddv58m12, 5' ...   609   0.0    2
(BJ424311) Dictyostelium discoideum cDNA clone:ddv52d04, 5' ...   609   0.0    2
dna update 2008.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        105   5e-21
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   2e-20
(Q9JLC8) RecName: Full=Sacsin;                                        103   2e-20
BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   102   2e-20
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   102   3e-20
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...    97   1e-18
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    91   7e-17
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    89   3e-16
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    76   3e-12
protein update 2009. 7.19
PSORT 

 psg: 0.68  gvh: 0.39  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHO809 is cyt
5' end seq. ID VHO809F
5' end seq. 
>VHO809F.Seq
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGACTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATTNNNNNNNNNN
Length of 5' end seq. 587
3' end seq. ID VHO809Z
3' end seq. 
>VHO809Z.Seq
NNNNNNNNNNTATACTCATAGCTTTCTTTACACGAGTGATCAAGTCTATGATCTCTTATT
CAAATATTATGGTAATAAGTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTT
TAGACAATTAATTTCATCAATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTC
AGATTTTATCACTAGTCTCAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAAT
AACAATTTGGAATTGTATCGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATAT
CCAATCATTACAAATTATCCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTT
TGATAAATCTTCATTGTTAATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAA
CAAACTAAAAATCTATTTAATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAA
TGCTCAAATCCCAATTTTATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAA
GGAAATCGATAAATTAAGAATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACA
AGATTATACTTCAATTTTTTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCA
AAAGTTTAGTAGTATTACAACCAGTAGTTACTGTGGGATTAGAAAAAATNATCAATANAA
ATTCCAGGATTAAAATTAATAAA
Length of 3' end seq. 743
Connected seq. ID VHO809P
Connected seq. 
>VHO809P.Seq
AAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAG
ATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAG
ATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGT
TGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAA
TTTTAATTTATAATAATGCAACTTTTCAAGAACATGACTGGGAAGGAATTTGTCAAATTA
GTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACA
GTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAG
ATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATA
AGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAA
AAGAATATTTTAATGGTACAATCATTCGTCTACCATT----------TATACTCATAGCT
TTCTTTACACGAGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAAGTTGG
CCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATCAATTT
GTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCTCAACT
CCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTATCGATG
AATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTATCCCAT
ATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTTAATGT
ACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTTAATAA
AAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTTATTAG
GCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAGAATAT
TTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTTTTATA
ATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTACAACCA
GTAGTTACTGTGGGATTAGAAAAAATNATCAATANAAATTCCAGGATTAAAATTAATAAA
Length of connected seq. 1310
Full length Seq ID -
Full length Seq. -
Length of full length seq. -