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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO782
Library AF
(Link to library)
Clone ID AFO782
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFO782P
(Link to Original site)
Representative DNA sequence 
>AFO782 (AFO782Q) /CSM/AF/AFO7-D/AFO782Q.Seq.d/
CACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACTXXXXXXXXXXCCNATCNTTTCA
ANTGAANANCCNTTTGATCAANATGATTGGGAATCATACCCTNAATTAACAGCNTCAGNT
GANATTCNAANTGNTGGNGATGATTTACTTGNTACCNACCCANAAAAAATTAANACTGGN
ATTGAAAAAAAAGCATGTAANGCTNTTTTATTAAAAGTTAACCNAATTGGNTCAGNCNCT
GAANCNATTNGNGCCGCTCTTGACNCAAANAANGCNTCATGGGGNGTCATGGNTANCCNT
CGNTCAGGTGAAACTGAANANACCNTCNNTGCTGANTTAGNTGTTGGNTTAGGGACNGGN
CNAATTAAAACTGGNGCNCCNTGTANATCANAAANAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
THLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILPVGAKDFNEAYRMGSEVYHN---

---PIXSXEXPFDQXDWESYPXLTASXXIXXXGDDLLXTXPXKIXTGIEKKACXAXLLKV
NXIGSXXEXIXAALDXXXASWGVMXXXRSGETEXTXXAXLXVGLGTGXIKTGAPCXSXX
Translated Amino Acid sequence (All Frames) 
Frame A:
hplitlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscss
ircinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsr
wypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftc
r**isyariydftswc*rfq*slsygfrsls*---

---PIXSXEXPFDQXDWESYPXLTASXXIXXXGDDLLXTXPXKIXTGIEKKACXAXLLKV
NXIGSXXEXIXAALDXXXASWGVMXXXRSGETEXTXXAXLXVGLGTGXIKTGAPCXSXX

Frame B:
THLLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVP
SGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLD
GTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHA
GNKLAMQEFMILPVGAKDFNEAYRMGSEVYHN---

---xsfqxxxxlixmignhtln*qxqxxfxxxxmiylxptxkklxlxlkkkhvxlfy*kl
txlxqxlxxxxplltqxxxhgxswxxxxqvklxxpsxlx*xlx*gxxxlklxxxvxxkx

Frame C:
ptyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfh
qvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sism
vpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmq
vin*lcknl*fyqlvlkismkpivwvqkfiit---

---xxfx*xxx*sx*lgiipxinsxx*xsxxwx*ftxyxpxkn*xwx*kksm*xxfiks*
pnwxxx*xxxxrs*xkxxxmgxhgxpsxr*n*xxxxc*xxcwxrdxxn*nwxxxxixx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO782 (AFO782Q) /CSM/AF/AFO7-D/AFO782Q.Seq.d/                       1483   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1211   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1211   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1211   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1211   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                       1211   0.0
AFE445 (AFE445Q) /CSM/AF/AFE4-B/AFE445Q.Seq.d/                       1211   0.0
AFD204 (AFD204Q) /CSM/AF/AFD2-A/AFD204Q.Seq.d/                       1211   0.0
AFG235 (AFG235Q) /CSM/AF/AFG2-B/AFG235Q.Seq.d/                       1203   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1199   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI504877|BI504877.1 BB170011A20G10.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20G10 5', mRNA sequence.   125  4e-31  2
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  2e-30  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  3e-27  6
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62  3e-27  5
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62  3e-27  5
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    62  3e-27  5
BD237140|BD237140.1 Staphylococcus aureus gene and polypeptide.    50  6e-27  6
AR282358|AR282358.1 Sequence 21 from patent US 6521441.           50  6e-27  6
AF065394|AF065394.1 Staphylococcus aureus enolase (eno) gene, complete cds.    50  6e-27  6
AR354089|AR354089.1 Sequence 207 from patent US 6593114.          50  7e-27  6
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   374   e-102
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   266   8e-70
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   265   1e-69
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   264   3e-69
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   264   4e-69
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   262   1e-68
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   261   2e-68
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   261   3e-68
BC017249_1(BC017249|pid:none) Homo sapiens enolase 3 (beta, musc...   260   6e-68
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   260   6e-68
protein update 2009. 4.26
PSORT 

 psg: 0.95  gvh: 0.51  alm: 0.32  top: 0.57  tms: 0.07  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFO782 is nuc
5' end seq. ID AFO782F
5' end seq. 
>AFO782F.Seq
CACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACT----------
Length of 5' end seq. 638
3' end seq. ID AFO782Z
3' end seq. 
>AFO782Z.Seq
----------CCNATCNTTTCAANTGAANANCCNTTTGATCAANATGATTGGGAATCATA
CCCTNAATTAACAGCNTCAGNTGANATTCNAANTGNTGGNGATGATTTACTTGNTACCNA
CCCANAAAAAATTAANACTGGNATTGAAAAAAAAGCATGTAANGCTNTTTTATTAAAAGT
TAACCNAATTGGNTCAGNCNCTGAANCNATTNGNGCCGCTCTTGACNCAAANAANGCNTC
ATGGGGNGTCATGGNTANCCNTCGNTCAGGTGAAACTGAANANACCNTCNNTGCTGANTT
AGNTGTTGGNTTAGGGACNGGNCNAATTAAAACTGGNGCNCCNTGTANATCANAAANAT
Length of 3' end seq. 349
Connected seq. ID AFO782P
Connected seq. 
>AFO782P.Seq
CACCCACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAAT
GAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGT
TGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCC
ATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAG
ATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGC
CGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGA
TGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGT
TTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGC
AGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGC
AGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAA
TGAAGCCTATCGTATGGGTTCAGAAGTTTATCATAACT----------CCNATCNTTTCA
ANTGAANANCCNTTTGATCAANATGATTGGGAATCATACCCTNAATTAACAGCNTCAGNT
GANATTCNAANTGNTGGNGATGATTTACTTGNTACCNACCCANAAAAAATTAANACTGGN
ATTGAAAAAAAAGCATGTAANGCTNTTTTATTAAAAGTTAACCNAATTGGNTCAGNCNCT
GAANCNATTNGNGCCGCTCTTGACNCAAANAANGCNTCATGGGGNGTCATGGNTANCCNT
CGNTCAGGTGAAACTGAANANACCNTCNNTGCTGANTTAGNTGTTGGNTTAGGGACNGGN
CNAATTAAAACTGGNGCNCCNTGTANATCANAAANAT
Length of connected seq. 987
Full length Seq ID -
Full length Seq. -
Length of full length seq. -