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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO779
Library AF
(Link to library)
Clone ID AFO779
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID AFO779E
(Link to Original site)
Representative DNA sequence 
>AFO779 (AFO779Q) /CSM/AF/AFO7-D/AFO779Q.Seq.d/
ATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAAT
TGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGA
CAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATG
GTGTGGCCATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACC
AGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCT
CTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCAC
TGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCA
CGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATC
AAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACC
ATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGC
CAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCAT
CTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAA
AGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAA
ACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGA
ACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACT
CGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTC
ATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGA
AATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATT
TGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGA
AAATAAAAAATAAAATAAAAGAAAAAAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
FHLLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFVKFYAPW
CGHCKKLAPDFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKIFDKSTT
AKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAP
WCGHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSK
DGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKEL
VKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEF
AKKLNILESFKSK*ikfnlenkk*nkrkk
Translated Amino Acid sequence (All Frames) 
Frame A:
isfinntstkneniiicyincigicclmlsrrkcccfitrql*ysc*wfkncickilctm
vwpl*ktctrf*npc*hirtsfqqscncqs*l*ps*qqssl**i*cfwlsnpqnl*qinh
cqrlqrc*is**iinlyq*srqnqr*skestikcc*fitikf*fscfr*ikecfs*ilct
mvwslqkvnarl*npw*hlrq*krcchrqn*lrcc**qshllqiwchwfpntqmvr*tkq
rr*ki*trs*s*yfhqlhqqtswc*pcqrw*trcwcwsc*ttryych*ihrcrc*sp*gt
rqessnrr*fsprrikn*rfilrqshenhc*kinrfcnh*nrsyhqislwfnvwkes**i
cqetqyfrifqikin*i*frk*kik*kkkk


Frame B:
FHLLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFVKFYAPW
CGHCKKLAPDFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKIFDKSTT
AKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAP
WCGHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSK
DGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKEL
VKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEF
AKKLNILESFKSK*ikfnlenkk*nkrkk


Frame C:
fiy**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttliqllmvqklyl*nsmhhg
vaivknlhqilksllthshqfptkl*lpklivtklttklsvvnmmflviqpsksltnqpl
pkittvldqlmny*lisiitpkptlk*rkhhqmlliyhhqiliqlf*inqrmf*lnsmhh
gvviaks*cqimkslvtptpmkkmlsspkliamllitkpsapnmvslvsqhsngsvnkak
tvknmnkvvililssttstnklvltvskvvnsllvlvvlnnsillplnsslpllksvrns
srklkpslilsqkn*elkvhttsks*kpllknqsil*plkslvspn*slvqclerklmnl
prnsif*nlsnqnklnli*kiknkikekk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFF762 (VFF762Q) /CSM/VF/VFF7-C/VFF762Q.Seq.d/                       2202   0.0
VFF615 (VFF615Q) /CSM/VF/VFF6-A/VFF615Q.Seq.d/                       2202   0.0
SFI534 (SFI534Q) /CSM/SF/SFI5-B/SFI534Q.Seq.d/                       2202   0.0
AHB158 (AHB158Q) /CSM/AH/AHB1-C/AHB158Q.Seq.d/                       2202   0.0
AFO779 (AFO779Q) /CSM/AF/AFO7-D/AFO779Q.Seq.d/                       2202   0.0
VFI769 (VFI769Q) /CSM/VF/VFI7-C/VFI769Q.Seq.d/                       2200   0.0
AFI744 (AFI744Q) /CSM/AF/AFI7-B/AFI744Q.Seq.d/                       2200   0.0
CFF440 (CFF440Q) /CSM/CF/CFF4-B/CFF440Q.Seq.d/                       2196   0.0
AFC888 (AFC888Q) /CSM/AF/AFC8-D/AFC888Q.Seq.d/                       2196   0.0
VFK244 (VFK244Q) /CSM/VF/VFK2-B/VFK244Q.Seq.d/                       2194   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  2175  0.0  3
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1471  0.0  8
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.     54  2e-19  4
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    58  3e-19  3
AU299541|AU299541.1 Cryptomeria japonica mRNA, clone: CM1065, 5' end, expressed in male cone.    62  3e-13  2
AK113243|AK113243.1 Ciona intestinalis cDNA, clone:ciad019c19, full insert sequence.    54  3e-13  5
CB090389|CB090389.1 gy74e10.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone gy74e10, mRNA sequence.    80  1e-12  2
CF508720|CF508720.1 USDA-FP_124000-223 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-70_B02 5', mRNA sequence.    68  3e-12  2
CF509886|CF509886.1 USDA-FP_125000-394 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-82_F01 5', mRNA sequence.    68  4e-12  2
BE208912|BE208912.1 GF-FV-P3F1 Marsh grapefruit young flavedo Citrus x paradisi cDNA, mRNA sequence.    68  4e-12  2
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   696   0.0
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   694   0.0
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   313   6e-84
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   303   6e-81
AY730725_1(AY730725|pid:none) Entamoeba histolytica protein disu...   301   2e-80
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   298   2e-79
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   298   2e-79
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   298   2e-79
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   295   2e-78
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   295   2e-78
protein update 2009. 6.29
PSORT 

 psg: 0.87  gvh: 0.77  alm: 0.45  top: 0.07  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for AFO779 is exc
5' end seq. ID AFO779F
5' end seq. 
>AFO779F.Seq
ATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAAT
TGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGA
CAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATG
GTGTGGCCATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACC
AGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCT
CTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCAC
TGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCA
CGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATC
AAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACC
ATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGC
CAATGAAAAAG----------
Length of 5' end seq. 611
3' end seq. ID AFO779Z
3' end seq. 
>AFO779Z.Seq
----------TAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATT
TTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGT
GTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATG
AAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCT
CCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACG
GTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAG
CTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAAC
TCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCA
AGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTCATACT
ACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGAAATCG
CTCGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATTTGCCA
AGAAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATA
AAAAATAAAATAAAAGAAAAAAAAA
Length of 3' end seq. 735
Connected seq. ID AFO779P
Connected seq. 
>AFO779P.Seq
ATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAAT
TGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGA
CAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATG
GTGTGGCCATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACC
AGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCT
CTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCAC
TGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCA
CGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATC
AAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACC
ATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGC
CAATGAAAAAG----------TAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATC
ACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTA
TGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACAC
CTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAA
AGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACA
AAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACAT
CAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCG
TGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAA
GGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGA
AGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAAC
CACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGA
TGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAA
TTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAAA
Length of connected seq. 1346
Full length Seq ID AFO779E
Full length Seq. 
>AFO779E.Seq
ATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAAT
TGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGA
CAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATG
GTGTGGCCATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACC
AGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCT
CTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCAC
TGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCA
CGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATC
AAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACC
ATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGC
CAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCAT
CTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAA
AGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAA
ACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGA
ACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACT
CGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAGGTTC
ATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCACTGA
AATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATGAATT
TGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATTTAGA
AAATAAAAAATAAAATAAAAGAAAAAAAAA
Length of full length seq. 1170