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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO672
Library VH
(Link to library)
Clone ID VHO672
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHO672P
(Link to Original site)
Representative DNA sequence 
>VHO672 (VHO672Q) /CSM/VH/VHO6-C/VHO672Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGXXXXXXXXX
XTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGA
TAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCT
CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGC
TTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTT
CAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCAT
CCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGA
ACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGT
ACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATT
CTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTC
CCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGT
TGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTT
AGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAATAATTTAAGTCGC
ACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVI---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ii
*valxfsxiesnlkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsl---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ii
*valxfsxiesnlkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqch---

---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknsk*f
kshxsfqx*nqi*kk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVI---

---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srtxvfkxrikskk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO672 (VHO672Q) /CSM/VH/VHO6-C/VHO672Q.Seq.d/                       2565   0.0
VHP730 (VHP730Q) /CSM/VH/VHP7-B/VHP730Q.Seq.d/                       1465   0.0
VHK162 (VHK162Q) /CSM/VH/VHK1-C/VHK162Q.Seq.d/                       1465   0.0
VHJ786 (VHJ786Q) /CSM/VH/VHJ7-D/VHJ786Q.Seq.d/                       1465   0.0
VHJ695 (VHJ695Q) /CSM/VH/VHJ6-D/VHJ695Q.Seq.d/                       1465   0.0
VHJ691 (VHJ691Q) /CSM/VH/VHJ6-D/VHJ691Q.Seq.d/                       1465   0.0
VHJ323 (VHJ323Q) /CSM/VH/VHJ3-A/VHJ323Q.Seq.d/                       1465   0.0
VHI690 (VHI690Q) /CSM/VH/VHI6-D/VHI690Q.Seq.d/                       1465   0.0
VHH822 (VHH822Q) /CSM/VH/VHH8-A/VHH822Q.Seq.d/                       1465   0.0
VHD569 (VHD569Q) /CSM/VH/VHD5-C/VHD569Q.Seq.d/                       1465   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445808) Dictyostelium discoideum cDNA clone:ddv60o18, 3' ...  1473   0.0    1
(BJ446334) Dictyostelium discoideum cDNA clone:ddv62l07, 3' ...  1465   0.0    1
(BJ443011) Dictyostelium discoideum cDNA clone:ddv51k15, 3' ...  1465   0.0    1
(BJ442773) Dictyostelium discoideum cDNA clone:ddv50m24, 3' ...  1465   0.0    1
(BJ442764) Dictyostelium discoideum cDNA clone:ddv50l21, 3' ...  1465   0.0    1
(BJ442731) Dictyostelium discoideum cDNA clone:ddv50e24, 3' ...  1465   0.0    1
(BJ442218) Dictyostelium discoideum cDNA clone:ddv49n05, 3' ...  1465   0.0    1
(BJ442075) Dictyostelium discoideum cDNA clone:ddv48c24, 3' ...  1465   0.0    1
(BJ441254) Dictyostelium discoideum cDNA clone:ddv46l06, 3' ...  1465   0.0    1
(BJ438830) Dictyostelium discoideum cDNA clone:ddv38i17, 3' ...  1465   0.0    1
dna update 2008.12.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   9e-81
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   3e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   300   1e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   298   4e-79
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   297   7e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   9e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   9e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   9e-79
protein update 2009. 7.19
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHO672 is cyt
5' end seq. ID VHO672F
5' end seq. 
>VHO672F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGNNNNNNNNN
N
Length of 5' end seq. 601
3' end seq. ID VHO672Z
3' end seq. 
>VHO672Z.Seq
NNNNNNNNNNTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCA
CGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGC
CAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTT
CGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGG
TATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGG
TCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAAC
TCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTAT
TTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTC
CCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGA
TTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTC
AATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAG
AAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAATAAT
TTAAGTCGCACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
Length of 3' end seq. 767
Connected seq. ID VHO672P
Connected seq. 
>VHO672P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTG---------
-TCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGA
TAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCT
CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGC
TTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTT
CAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCAT
CCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGA
ACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGT
ACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATT
CTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTC
CCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGT
TGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTT
AGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAATAATTTAAGTCGC
ACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
Length of connected seq. 1348
Full length Seq ID -
Full length Seq. -
Length of full length seq. -