>AFO764 (AFO764Q) /CSM/AF/AFO7-C/AFO764Q.Seq.d/
ACAGTGGTGATGATGTATCAGCAATCGTTATTGATGTTGGAACCTTTTCAACAAAAGGTG
GTTATGCTGGTGAAGATTCACCAAAAGCAGTATTTCCAACTGATATAGGTGTTGTATATA
AAAACGAGAATGAAACAGTTGGAACAGGTGATTCTGAAATGGGTGAAAAAGATGATTCAG
AACCAAAGAGAACTTATTATTGTGGTACAAATGGTATCACTTATAGAAGACCACATATGG
AGATCATTAATCCTTTATCCGATGGTTTAATTAAAAATTGGGATGCGATGGAACAAATTT
GGAATCATACTTTCTATGAAAGATTACATATCAACCCAATTGAACATCCAATATTATTAG
CAGAACCAACATATAATACAAGAGCAATTAGAGAGAAAGTAACAGAGATTATGTTTGAAA
AATATCATGCACCAGCATTATTCATTGCAAAGAATGCTGTTTTATCTTCATTTGCAAGTT
CAAAAGCATCTTCATTAGTATTAGATTCTGGTGGTGGTATGACTTCTGTCACACCTGTAT
TTGAAGGTTTTGTTGTTAAAAATGGTATTGTAAAATCAAATTTAGCAGGTAATAGATTAA
CAGAAGAAXXXXXXXXXX

SGDDVSAIVIDVGTFSTKGGYAGEDSPKAVFPTDIGVVYKNENETVGTGDSEMGEKDDSE
PKRTYYCGTNGITYRRPHMEIINPLSDGLIKNWDAMEQIWNHTFYERLHINPIEHPILLA
EPTYNTRAIREKVTEIMFEKYHAPALFIAKNAVLSSFASSKASSLVLDSGGGMTSVTPVF
EGFVVKNGIVKSNLAGNRLTE---

Frame A:
tvvmmyqqsllmlepfqqkvvmlvkihqkqyfqli*vlyiktrmkqleqvilkwvkkmiq
nqreliivvqmvsliedhiwrslilypmv*lkigmrwnkfgiilsmkdyistqlniqyy*
qnqhiiqeqlerk*qrlclknimhqhyslqrmlfylhlqvqkhlh*y*ilvvv*llshly
lkvlllkmvl*nqi*qvid*qk---


Frame B:
qw**cisnry*cwnlfnkrwlcw*rftkssisn*yrcci*kre*nswnr*f*ng*kr*fr
tkenlllwykwyhl*kttygdh*sfirwfn*klgcdgtnlesyfl*kityqpn*tsniis
rtni*yksn*resnrdyv*kisctsiihckeccfifickfksifisirfwwwydfchtci
*rfcc*kwyckikfsr**inrr---


Frame C:
SGDDVSAIVIDVGTFSTKGGYAGEDSPKAVFPTDIGVVYKNENETVGTGDSEMGEKDDSE
PKRTYYCGTNGITYRRPHMEIINPLSDGLIKNWDAMEQIWNHTFYERLHINPIEHPILLA
EPTYNTRAIREKVTEIMFEKYHAPALFIAKNAVLSSFASSKASSLVLDSGGGMTSVTPVF
EGFVVKNGIVKSNLAGNRLTE---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO764 (AFO764Q) /CSM/AF/AFO7-C/AFO764Q.Seq.d/                       1205   0.0
SLK622 (SLK622Q) /CSM/SL/SLK6-A/SLK622Q.Seq.d/                       1189   0.0
CFI624 (CFI624Q) /CSM/CF/CFI6-A/CFI624Q.Seq.d/                       1090   0.0
CFI572 (CFI572Q) /CSM/CF/CFI5-C/CFI572Q.Seq.d/                        460   e-128
AFG296 (AFG296Q) /CSM/AF/AFG2-D/AFG296Q.Seq.d/                        100   5e-20
AFM734 (AFM734Q) /CSM/AF/AFM7-B/AFM734Q.Seq.d/                         66   7e-10
SFC783 (SFC783Q) /CSM/SF/SFC7-D/SFC783Q.Seq.d/                         60   4e-08
VFM611 (VFM611Q) /CSM/VF/VFM6-A/VFM611Q.Seq.d/                         42   0.010
VFM139 (VFM139Q) /CSM/VF/VFM1-B/VFM139Q.Seq.d/                         42   0.010
VFJ707 (VFJ707Q) /CSM/VF/VFJ7-A/VFJ707Q.Seq.d/                         42   0.010

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ132371|AJ132371.1 Allogromia sp. mRNA for actin 2.              40  3e-05  3
AF527915|AF527915.1 Saccharomyces unisporus NRRL Y-1556 actin (act1) gene, partial cds.    46  3e-04  3
AF527917|AF527917.1 Saccharomyces transvaalensis NRRL Y-17245 actin (act1) gene, partial cds.    52  8e-04  2
BQ116536|BQ116536.2 EST602112 mixed potato tissues Solanum tuberosum cDNA clone STMDI72 5' end, mRNA sequence.    36  0.023  2
AL132960|AL132960.2 Arabidopsis thaliana DNA chromosome 3, BAC clone F5K20.    42  0.028  6
BG592325|BG592325.1 EST491003 cSTS Solanum tuberosum cDNA clone cSTS1A21 5' sequence, mRNA sequence.    36  0.028  2
AF527937|AF527937.1 Saccharomyces dairenensis NRRL Y-12639 actin (act1) gene, partial cds.    44  0.040  2
AF527916|AF527916.1 Kluyveromyces africanus NRRL Y-8276 actin (act1) gene, partial cds.    36  0.18  3
AF101729|AF101729.1 Naegleria gruberi actin (act1) gene, complete cds.    44  0.19  2
AJ132370|AJ132370.1 Allogromia sp. mRNA for actin 1.              38  0.27  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC152123_6(AC152123|pid:none) Genomic sequence for broccoli [Bra...   187   1e-46
(Q84M92) RecName: Full=Actin-related protein 4;  &AK228098_1(AK2...   187   3e-46
AY087788_1(AY087788|pid:none) Arabidopsis thaliana clone 38419 m...   186   3e-46
BT038602_1(BT038602|pid:none) Zea mays full-length cDNA clone ZM...   181   1e-44
BT053205_1(BT053205|pid:none) Medicago truncatula clone MTYFL_FM...   178   9e-44
BT044645_1(BT044645|pid:none) Salmo salar clone ssal-rgf-001-293...   159   6e-38
AY394958_1(AY394958|pid:none) Danio rerio clone RK053A3F02 BAF53...   159   7e-38
BC045889_1(BC045889|pid:none) Danio rerio BRG1/brm-associated fa...   159   7e-38
BC064183_1(BC064183|pid:none) Xenopus tropicalis actin-like 6A, ...   158   1e-37
(Q4R333) RecName: Full=Actin-like protein 6A;  &AB179435_1(AB179...   157   2e-37

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFO764 is cyt

>AFO764F.Seq
ACAGTGGTGATGATGTATCAGCAATCGTTATTGATGTTGGAACCTTTTCAACAAAAGGTG
GTTATGCTGGTGAAGATTCACCAAAAGCAGTATTTCCAACTGATATAGGTGTTGTATATA
AAAACGAGAATGAAACAGTTGGAACAGGTGATTCTGAAATGGGTGAAAAAGATGATTCAG
AACCAAAGAGAACTTATTATTGTGGTACAAATGGTATCACTTATAGAAGACCACATATGG
AGATCATTAATCCTTTATCCGATGGTTTAATTAAAAATTGGGATGCGATGGAACAAATTT
GGAATCATACTTTCTATGAAAGATTACATATCAACCCAATTGAACATCCAATATTATTAG
CAGAACCAACATATAATACAAGAGCAATTAGAGAGAAAGTAACAGAGATTATGTTTGAAA
AATATCATGCACCAGCATTATTCATTGCAAAGAATGCTGTTTTATCTTCATTTGCAAGTT
CAAAAGCATCTTCATTAGTATTAGATTCTGGTGGTGGTATGACTTCTGTCACACCTGTAT
TTGAAGGTTTTGTTGTTAAAAATGGTATTGTAAAATCAAATTTAGCAGGTAATAGATTAA
CAGAAGAA----------