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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO532
Library VH
(Link to library)
Clone ID VHO532
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16015-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHO532 (VHO532Q) /CSM/VH/VHO5-B/VHO532Q.Seq.d/
XXXXXXXXXXTTTGTTAATCGTGCCACCGATGTNTCACGTTGGATCGTCCANACCGTACT
AAATGAAAGTCGTGATAAAAAGANCAAAATGAAAATGTTGGATANNTTCANANAGNCNAC
CGAATGTCTTAGACAATTGNATCNGTCTTTAGTCTCCAATATTACATTGATGGTGCTTGG
AGAAATCTTCCAACTTTCAAAAACGATGTTT
sequence update 2002. 9.10
Translated Amino Acid sequence 
---VNRATDVSRWIVXTVLNESRDKKXKMKMLDXFXXXTECLRQLXXSLVSNITLMVLGE
IFQLSKTMF
Translated Amino Acid sequence (All Frames) 
Frame A:
---llivppmxhvgssxpy*mkvvikrxk*kcwixsxxxpnvldnxixl*spilh*wcle
kssnfqkrc


Frame B:
---c*schrcxtldrpxrtk*ks**kxqnenvgxxxxxxrms*tixsvfslqyyidgawr
nlptfkndv


Frame C:
---VNRATDVSRWIVXTVLNESRDKKXKMKMLDXFXXXTECLRQLXXSLVSNITLMVLGE
IFQLSKTMF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO532 (VHO532Q) /CSM/VH/VHO5-B/VHO532Q.Seq.d/                        333   6e-91
VSF484 (VSF484Q) /CSM/VS/VSF4-D/VSF484Q.Seq.d/                        135   3e-31
VSD795 (VSD795Q) /CSM/VS/VSD7-D/VSD795Q.Seq.d/                        135   3e-31
SSG835 (SSG835Q) /CSM/SS/SSG8-B/SSG835Q.Seq.d/                        135   3e-31
SSD424 (SSD424Q) /CSM/SS/SSD4-A/SSD424Q.Seq.d/                        135   3e-31
SSB193 (SSB193Q) /CSM/SS/SSB1-D/SSB193Q.Seq.d/                        135   3e-31
SSH250 (SSH250Q) /CSM/SS/SSH2-C/SSH250Q.Seq.d/                        121   4e-27
VHA595 (VHA595Q) /CSM/VH/VHA5-D/VHA595Q.Seq.d/                        119   2e-26
SHC314 (SHC314Q) /CSM/SH/SHC3-A/SHC314Q.Seq.d/                         98   6e-20
AHC344 (AHC344Q) /CSM/AH/AHC3-B/AHC344Q.Seq.d/                         66   2e-10
own update 2003. 1. 6
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY160095|AY160095.1 Dictyostelium discoideum nucleotide exchange factor RasGEF F (gefF) gene, complete cds.   202   1e-48  1
AC131807|AC131807.4 Rattus norvegicus clone CH230-374B11, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    32   4.2    5
AC090143|AC090143.4 Homo sapiens chromosome 8 clone RP11-263C6 map 8, LOW-PASS SEQUENCE SAMPLING.    34   7.5    2
AC036130|AC036130.3 Homo sapiens chromosome 4 clone RP11-526M1 map 4, WORKING DRAFT SEQUENCE, 5 unordered pieces.    40   12     1
AP000478|AP000478.6 Homo sapiens genomic DNA, chromosome 11 clone:CMB9-14E22, complete sequence.    40   12     1
dna update 2003.10.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY160095_1(AY160095|pid:none) Dictyostelium discoideum nucleotid...    62   8e-09
protein update 2009. 3.17
PSORT 

 psg: 0.85  gvh: 0.48  alm: 0.38  top: 0.60  tms: 0.00  mit: 0.34  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHO532 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHO532Z
3' end seq. 
>VHO532Z.Seq
NNNNNNNNNNTTTGTTAATCGTGCCACCGATGTNTCACGTTGGATCGTCCANACCGTACT
AAATGAAAGTCGTGATAAAAAGANCAAAATGAAAATGTTGGATANNTTCANANAGNCNAC
CGAATGTCTTAGACAATTGNATCNGTCTTTAGTCTCCAATATTACATTGATGGTGCTTGG
AGAAATCTTCCAACTTTCAAAAACGATGTTT
Length of 3' end seq. 211
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -