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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO756
Library AF
(Link to library)
Clone ID AFO756
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16561-1
Original site URL
Representative seq. ID AFO756Z
(Link to Original site)
Representative DNA sequence 
>AFO756 (AFO756Q) /CSM/AF/AFO7-C/AFO756Q.Seq.d/
XXXXXXXXXXGAAGTTNAAGGGTAGTNNCAACATTGANAATGTAAAANNCAAANATNCAA
GATAAAGAAGGTNTTCCNCCAGANCNNCAACGTCTCANTTCGNTGGTANACAATTANAAN
ATGGTCGTACTCTCTCTGATTACAAAATCCANAAAGAATCANCTCTCCACTTGGTCCTCA
GATTAANAAGGTGGTATGCAAATNTTTGTCAAAACATTAACTGGTAAGACCATCACTCTT
NAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGT
ATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACT
CTCTNTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGT
GGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTACACTNGAAGTTGAAGGT
TNTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGAT
CAACAACGTCTCATTTTCGCCGGTAAACAATTGNAGGATGGTCGTACTCTCTCTGATTAC
AACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATT
TTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATT
GAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATNCCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---sxrvvxtlxm*xxxxqdkegxppxxqrlxsxvxnxxmvvlslitksxknxlstwssd
*XGGMQXFVKTLTGKTITLXVEGSDNIENVKTKIQDKEGIPPDQQRLIFAGKQLEDGRTL
XDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEGXDNIENVKAKIQDKEGIPPDQ
QRLIFAGKQLXDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIE
NVKTKIQDKEGXP
Translated Amino Acid sequence (All Frames) 
Frame A:
---kxkgsxnixnvkxkxxr*rrxsxrxxtsxfxgxqlxxgrtlsdykixkesxlhlvlr
lxrwyanxcqninw*dhhsxs*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsys
l*lqhskgihspfspqikrwyanfcqnphw*dyytxs*rx**h*ec*sqnprqrrystrs
ttshfrr*tixgwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*
ecknknsrqrryx


Frame B:
---sxrvvxtlxm*xxxxqdkegxppxxqrlxsxvxnxxmvvlslitksxknxlstwssd
*XGGMQXFVKTLTGKTITLXVEGSDNIENVKTKIQDKEGIPPDQQRLIFAGKQLEDGRTL
XDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEGXDNIENVKAKIQDKEGIPPDQ
QRLIFAGKQLXDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEGSDNIE
NVKTKIQDKEGXP


Frame C:
---vxg*xqh*xckxqxxkikkvfxqxxnvsxrwxtixxwsysl*lqnpxrixsplgpqi
xkvvckxlskh*lvrpslxklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvls
xittfkrnplsi*ssd*evvckflskpslvrllhxklkvxitlrmlkpksktkkvfhqin
nvsfspvnnxrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlr
m*kqkfktkkvx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO756 (AFO756Q) /CSM/AF/AFO7-C/AFO756Q.Seq.d/                       1324   0.0
CFA741 (CFA741Q) /CSM/CF/CFA7-B/CFA741Q.Seq.d/                       1269   0.0
CFF682 (CFF682Q) /CSM/CF/CFF6-D/CFF682Q.Seq.d/                       1263   0.0
SFG568 (SFG568Q) /CSM/SF/SFG5-C/SFG568Q.Seq.d/                       1259   0.0
SFF543 (SFF543Q) /CSM/SF/SFF5-B/SFF543Q.Seq.d/                       1259   0.0
CFH301 (CFH301Q) /CSM/CF/CFH3-A/CFH301Q.Seq.d/                       1259   0.0
CFJ273 (CFJ273Q) /CSM/CF/CFJ2-D/CFJ273Q.Seq.d/                       1257   0.0
CFF858 (CFF858Q) /CSM/CF/CFF8-C/CFF858Q.Seq.d/                       1257   0.0
CFF257 (CFF257Q) /CSM/CF/CFF2-C/CFF257Q.Seq.d/                       1257   0.0
AFK729 (AFK729Q) /CSM/AF/AFK7-B/AFK729Q.Seq.d/                       1257   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1088  0.0  3
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1072  0.0  3
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1072  0.0  3
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   618  0.0  3
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   654  0.0  4
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   638  e-179  4
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   486  e-156  4
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   486  e-155  4
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   172  2e-85  5
Z54197|Z54197.1 C.albicans UBI4 gene encoding polyubiquitin.     125  1e-83  6
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF285161_1(AF285161|pid:none) Mus musculus cell-line C3H/He poly...   344   e-100
A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   364   2e-99
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   364   2e-99
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   364   2e-99
M17597_1(M17597|pid:none) Human poly-ubiquitin mRNA, complete cds.    341   4e-99
AF088848_1(AF088848|pid:none) Capsicum chinense polyubiquitin (U...   341   1e-98
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   360   3e-98
CR954212_116(CR954212|pid:none) Ostreococcus tauri strain OTTH05...   339   4e-98
Z28649_1(Z28649|pid:none) Acetabularia cliftonii gene for polyub...   345   1e-97
A29584(A29584) ubiquitin precursor - pig (fragment)  &M18159_1(M...   341   3e-97
protein update 2009. 4.26
PSORT 

 psg: 0.78  gvh: 0.37  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 1.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for AFO756 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFO756Z
3' end seq. 
>AFO756Z.Seq
----------GAAGTTNAAGGGTAGTNNCAACATTGANAATGTAAAANNCAAANATNCAA
GATAAAGAAGGTNTTCCNCCAGANCNNCAACGTCTCANTTCGNTGGTANACAATTANAAN
ATGGTCGTACTCTCTCTGATTACAAAATCCANAAAGAATCANCTCTCCACTTGGTCCTCA
GATTAANAAGGTGGTATGCAAATNTTTGTCAAAACATTAACTGGTAAGACCATCACTCTT
NAAGTTGAGGGCTCAGATAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGT
ATCCCACCAGACCAACAAAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACT
CTCTNTGATTACAACATTCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGT
GGTATGCAAATTTTTGTCAAAACCCTCACTGGTAAGACTATTACACTNGAAGTTGAAGGT
TNTGATAACATTGAGAATGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGAT
CAACAACGTCTCATTTTCGCCGGTAAACAATTGNAGGATGGTCGTACTCTCTCTGATTAC
AACATTCAAAAGGAATCCACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATT
TTTGTAAAAACTCTCACTGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATT
GAGAATGTAAAAACAAAAATTCAAGACAAAGAAGGTATNCCA
Length of 3' end seq. 752
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -