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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO325
Library VH
(Link to library)
Clone ID VHO325
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16497-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHO325 (VHO325Q) /CSM/VH/VHO3-B/VHO325Q.Seq.d/
XXXXXXXXXXCTGATAAATGTATCCAATTCTCTTGTAATGAAGCCACCGGTTGCCAACAA
TCAGCTGTCGNTTGTCCAGATGTTGGTAGTTGTTTAGTTGGTTATTGTCATAGTAAACAA
GGTTGTCTCACCAAACCAAGATCNTGCGATACTGGTGTTTTCTGTTTANCTGGTGAATGT
ATTNAAAATGCTGGTGGTTGTATCGTCTATGAAAAACGTTGTGATGNTGATAATGNAANA
TGTCAACAAGAGTGTATGTGTAAATAATACCGCCATCTGAAGAAGGTTATTGTAAATCTG
AAGCCTTATGATCCANNACCANTCATTTGTANAACTGGTGCTGTCGTTTCAACTGCTGTC
ATTGNTGGTGTCAC
sequence update 2002. 9.10
Translated Amino Acid sequence 
---DKCIQFSCNEATGCQQSAVXCPDVGSCLVGYCHSKQGCLTKPRSCDTGVFCLXGECI
XNAGGCIVYEKRCDXDNXXCQQECMCK*yrhlkkvivnlkpydpxpxicxtgavvstavi
xgv
Translated Amino Acid sequence (All Frames) 
Frame A:
---**mypill**shrlptiscrlsrcw*lfswlls**trlshqtkixrywcflfxw*my
xkcwwlyrl*ktl*x**xxmstrvyv*iippseegyckseal*sxtxhlxnwccrfncch
xwch


Frame B:
---DKCIQFSCNEATGCQQSAVXCPDVGSCLVGYCHSKQGCLTKPRSCDTGVFCLXGECI
XNAGGCIVYEKRCDXDNXXCQQECMCK*yrhlkkvivnlkpydpxpxicxtgavvstavi
xgv


Frame C:
---invsnslvmkppvannqlsxvqmlvvv*lvivivnkvvspnqdxailvfsvxlvnvx
kmlvvvssmknvvmximxxvnksvcvnntai*rrll*i*slmixxxsfvxlvlsfqllsl
xvs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO325 (VHO325Q) /CSM/VH/VHO3-B/VHO325Q.Seq.d/                        650   0.0
VHA244 (VHA244Q) /CSM/VH/VHA2-B/VHA244Q.Seq.d/                        611   e-174
VHP473 (VHP473Q) /CSM/VH/VHP4-D/VHP473Q.Seq.d/                        589   e-167
VHQ221 (VHQ221Q) /CSM/VH/VHQ2-A/VHQ221Q.Seq.d/                        587   e-167
VHQ163 (VHQ163Q) /CSM/VH/VHQ1-C/VHQ163Q.Seq.d/                        587   e-167
VHP731 (VHP731Q) /CSM/VH/VHP7-B/VHP731Q.Seq.d/                        587   e-167
VHP644 (VHP644Q) /CSM/VH/VHP6-B/VHP644Q.Seq.d/                        587   e-167
VHP362 (VHP362Q) /CSM/VH/VHP3-C/VHP362Q.Seq.d/                        587   e-167
VHO544 (VHO544Q) /CSM/VH/VHO5-B/VHO544Q.Seq.d/                        587   e-167
VHO307 (VHO307Q) /CSM/VH/VHO3-A/VHO307Q.Seq.d/                        587   e-167
own update 2004. 8. 5
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.   426   e-160  4
BE064941|BE064941.1 RC1-BT0313-130400-016-f04 BT0313 Homo sapiens cDNA, mRNA sequence.    38   1.3    2
CB077403|CB077403.1 hj53c10.g1 Hedyotis terminalis flower - Stage 2 (NYBG) Hedyotis terminalis cDNA clone hj53c10, mRNA sequence.    38   4.2    2
CB076908|CB076908.1 hj46b11.g1 Hedyotis terminalis flower - Stage 2 (NYBG) Hedyotis terminalis cDNA clone hj46b11, mRNA sequence.    38   4.3    2
AE011271|AE011271.1 Leptospira interrogans serovar lai str. 56601 chromosome I, section 80 of 397 of the complete sequence.    42   5.5    1
CK289032|CK289032.1 EST751754 Nicotiana benthamiana mixed tissue cDNA library, normalized, full-length Nicotiana benthamiana cDNA clone NBMBK94 5' end, mRNA sequence.    42   5.5    1
dna update 2004. 3.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   189   2e-47
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   106   2e-22
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                 90   3e-17
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                 88   1e-16
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...    82   6e-15
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                 72   6e-12
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 61   1e-08
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    56   3e-07
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    56   4e-07
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    54   2e-06
protein update 2009. 5.18
PSORT 

 psg: 0.75  gvh: 0.53  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VHO325 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHO325Z
3' end seq. 
>VHO325Z.Seq
NNNNNNNNNNCTGATAAATGTATCCAATTCTCTTGTAATGAAGCCACCGGTTGCCAACAA
TCAGCTGTCGNTTGTCCAGATGTTGGTAGTTGTTTAGTTGGTTATTGTCATAGTAAACAA
GGTTGTCTCACCAAACCAAGATCNTGCGATACTGGTGTTTTCTGTTTANCTGGTGAATGT
ATTNAAAATGCTGGTGGTTGTATCGTCTATGAAAAACGTTGTGATGNTGATAATGNAANA
TGTCAACAAGAGTGTATGTGTAAATAATACCGCCATCTGAAGAAGGTTATTGTAAATCTG
AAGCCTTATGATCCANNACCANTCATTTGTANAACTGGTGCTGTCGTTTCAACTGCTGTC
ATTGNTGGTGTCAC
Length of 3' end seq. 374
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -