NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO729
Library AF
(Link to library)
Clone ID AFO729
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15674-1
Original site URL
Representative seq. ID AFO729P
(Link to Original site)
Representative DNA sequence 
>AFO729 (AFO729Q) /CSM/AF/AFO7-B/AFO729Q.Seq.d/
GACAATCCATTATTATTTTTATATATAAGTAATGTTTTGATTAAAAATATAATTATATCC
ATTTTTTTATTTCAATTTTTTTATTATTTTTTTTTTTGGTTTTNATTTTTTTTTTTTTAN
TTTTTTANTTTTTTTTTNGGNTAAAAAAAAAAXXXXXXXXXXGTCCACAAAGAGGTGGTC
AAGGTGAAACCAAATCATGGACTCCAGTCACCAAATTAGGTCGTCTCGTTGCTCTTGGTT
TAGTCGAGAATATCCACGAAATCTATCTTTTCTCTTTACCAATCAAGGAGTACCAAATCA
TTGATAAATTCCTTAACCTCAAGGATGAAGTTATGAAGATTGTTCCAGTTCAAAAGCAAA
CTCGTGCTGGTCAACGTACCCGTTTCAAGGCTTTCGTCGTTGTTGGTGATCACAACGGTC
ATGTAGGTCTCGGTGTTAAATGCGCTAAGGAAGTTGCCACCGCCATTTCTGGTGCTATCG
TTGCTGCCAAATTATCAGTCATCCCAGTTCGTCGTGGTTATTGGGGTAACAAACTCGGTG
CTCCACACACTGTCCCAACCAAAGTCACTGGTAAATGCGGTTCCGTCGCTGTACGTCTCG
TTCCAGCCCCAAGAGGTACTGGTATCGTTGCTGCTCGTGTTCCAAAGAAATTACTCCAAT
ATGCTGGTGTTGATGATGTTTACACATCTTCACGTGGTAAGACCAGAACCATGGGTAACT
TTGTCATGGCCACTTTCTTCGCCATCACCAAGACCTTTGCTTACTTAACCCCAGATCTTT
GGAGAGAAGTCAAATTAATCAAGACTCCATACCAAGAACACTCTGCCAAATTATCCGAAA
AACAACAATAAATCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
qsiiifiyk*cfd*KYNYIHFFISIFLLFFFLVXIFFFLXFXFFFXXKKK---

---PQRGGQGETKSWTPVTKLGRLVALGLVENIHEIYLFSLPIKEYQIIDKFLNLKDEVM
KIVPVQKQTRAGQRTRFKAFVVVGDHNGHVGLGVKCAKEVATAISGAIVAAKLSVIPVRR
GYWGNKLGAPHTVPTKVTGKCGSVAVRLVPAPRGTGIVAARVPKKLLQYAGVDDVYTSSR
GKTRTMGNFVMATFFAITKTFAYLTPDLWREVKLIKTPYQEHSAKLSEKQQ*i
Translated Amino Acid sequence (All Frames) 
Frame A:
dnpllflyisnvlikniiisiflfqffyyfffwfxffffxffxffxg*kk---

---vhkevvkvkpnhglqspn*vvslllv*sristksifslyqsrstkslinsltsrmkl
*rlfqfksklvlvnvpvsrlssllvittvm*vsvlnalrklpppflvlsllpnyqssqfv
vvigvtnsvlhtlsqpkslvnavpslyvsfqpqevlvslllvfqrnysnmlvlmmfthlh
vvrpepwvtlswplsspsprpllt*pqifgeksn*srlhtkntlpnypknnnks

Frame B:
tihyyfyi*vmf*lki*lypffyfnffiifffgfxffffxflxffxxkkk---

---stkrwsr*nqimdsshqirssrcswfsreyprnlsflftnqgvpnh**ip*pqg*sy
edcssskanscwstypfqgfrrcw*sqrscrsrc*mr*gschrhfwcyrccqiishpsss
wllg*qtrcsthcpnqshw*mrfrrctsrsspkrywyrccscskeitpicwc**clhift
w*dqnhg*lchghflrhhqdlcllnprslersqinqdsiprtlcqiirkttin

Frame C:
qsiiifiyk*cfd*KYNYIHFFISIFLLFFFLVXIFFFLXFXFFFXXKKK---

---PQRGGQGETKSWTPVTKLGRLVALGLVENIHEIYLFSLPIKEYQIIDKFLNLKDEVM
KIVPVQKQTRAGQRTRFKAFVVVGDHNGHVGLGVKCAKEVATAISGAIVAAKLSVIPVRR
GYWGNKLGAPHTVPTKVTGKCGSVAVRLVPAPRGTGIVAARVPKKLLQYAGVDDVYTSSR
GKTRTMGNFVMATFFAITKTFAYLTPDLWREVKLIKTPYQEHSAKLSEKQQ*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC570 (VHC570Q) /CSM/VH/VHC5-C/VHC570Q.Seq.d/                       1374   0.0
VHC440 (VHC440Q) /CSM/VH/VHC4-B/VHC440Q.Seq.d/                       1374   0.0
SLE490 (SLE490Q) /CSM/SL/SLE4-D/SLE490Q.Seq.d/                       1374   0.0
AFO729 (AFO729Q) /CSM/AF/AFO7-B/AFO729Q.Seq.d/                       1374   0.0
VHN687 (VHN687Q) /CSM/VH/VHN6-D/VHN687Q.Seq.d/                       1352   0.0
VFE388 (VFE388Q) /CSM/VF/VFE3-D/VFE388Q.Seq.d/                       1344   0.0
FC-BD02 (FC-BD02Q) /CSM/FC/FC-BD/FC-BD02Q.Seq.d/                     1217   0.0
SLC152 (SLC152Q) /CSM/SL/SLC1-C/SLC152Q.Seq.d/                       1164   0.0
FC-AC19 (FC-AC19Q) /CSM/FC/FC-AC/FC-AC19Q.Seq.d/                     1152   0.0
FC-BA12 (FC-BA12Q) /CSM/FC/FC-BA/FC-BA12Q.Seq.d/                     1136   0.0
own update 2002.11. 5
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X56297|X56297.1 D.discoideum LLRep3 mRNA.                       1366  0.0  1
AU011370|AU011370.1 Schizosaccharomyces pombe cDNA, clone spc11190.    96  1e-32  6
AU010208|AU010208.1 Schizosaccharomyces pombe cDNA, clone spc05562.   109  4e-30  4
AU006568|AU006568.1 Schizosaccharomyces pombe cDNA, clone spc00131.   101  1e-29  4
AU011889|AU011889.1 Schizosaccharomyces pombe cDNA, clone spc06061.   109  1e-29  4
AU008028|AU008028.1 Schizosaccharomyces pombe cDNA, clone spc02783.   109  4e-28  3
AL031798|AL031798.1 S.pombe chromosome III cosmid c576.          109  1e-27  6
AU013664|AU013664.1 Schizosaccharomyces pombe cDNA, clone spc08473.   105  8e-27  3
AX537010|AX537010.1 Sequence 611 from Patent WO02064766.          44  7e-21  6
AX489195|AX489195.1 Sequence 6495 from Patent WO02053728.         44  7e-21  6
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P27685) RecName: Full=40S ribosomal protein S2; AltName: Full=R...   457   e-127
AY085726_1(AY085726|pid:none) Arabidopsis thaliana clone 17415 m...   335   1e-90
AY961548_1(AY961548|pid:none) Lysiphlebus testaceipes ribosomal ...   334   2e-90
EF146355_1(EF146355|pid:none) Populus trichocarpa clone WS0117_H...   333   4e-90
AC135958_8(AC135958|pid:none) Oryza sativa chromosome 3 BAC OSJN...   333   5e-90
AY088743_1(AY088743|pid:none) Arabidopsis thaliana clone 93867 m...   333   5e-90
AB008017_1(AB008017|pid:none) Arabidopsis thaliana RF12 mRNA, pa...   333   5e-90
(Q93VB8) RecName: Full=40S ribosomal protein S2-2;  &AB076984_1(...   333   5e-90
(Q8L8Y0) RecName: Full=40S ribosomal protein S2-1;  &AB008016_1(...   333   5e-90
(Q9SCM3) RecName: Full=40S ribosomal protein S2-4;  &AK228054_1(...   331   2e-89
protein update 2009. 4.26
PSORT 

 psg: 1.03  gvh: 0.67  alm: 0.31  top: 0.63  tms: 0.07  mit: 0.25  mip: 0.10
 nuc: 0.03  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for AFO729 is exc
5' end seq. ID AFO729F
5' end seq. 
>AFO729F.Seq
GACAATCCATTATTATTTTTATATATAAGTAATGTTTTGATTAAAAATATAATTATATCC
ATTTTTTTATTTCAATTTTTTTATTATTTTTTTTTTTGGTTTTNATTTTTTTTTTTTTAN
TTTTTTANTTTTTTTTTNGGNTAAAAAAAAAA----------
Length of 5' end seq. 152
3' end seq. ID AFO729Z
3' end seq. 
>AFO729Z.Seq
----------GTCCACAAAGAGGTGGTCAAGGTGAAACCAAATCATGGACTCCAGTCACC
AAATTAGGTCGTCTCGTTGCTCTTGGTTTAGTCGAGAATATCCACGAAATCTATCTTTTC
TCTTTACCAATCAAGGAGTACCAAATCATTGATAAATTCCTTAACCTCAAGGATGAAGTT
ATGAAGATTGTTCCAGTTCAAAAGCAAACTCGTGCTGGTCAACGTACCCGTTTCAAGGCT
TTCGTCGTTGTTGGTGATCACAACGGTCATGTAGGTCTCGGTGTTAAATGCGCTAAGGAA
GTTGCCACCGCCATTTCTGGTGCTATCGTTGCTGCCAAATTATCAGTCATCCCAGTTCGT
CGTGGTTATTGGGGTAACAAACTCGGTGCTCCACACACTGTCCCAACCAAAGTCACTGGT
AAATGCGGTTCCGTCGCTGTACGTCTCGTTCCAGCCCCAAGAGGTACTGGTATCGTTGCT
GCTCGTGTTCCAAAGAAATTACTCCAATATGCTGGTGTTGATGATGTTTACACATCTTCA
CGTGGTAAGACCAGAACCATGGGTAACTTTGTCATGGCCACTTTCTTCGCCATCACCAAG
ACCTTTGCTTACTTAACCCCAGATCTTTGGAGAGAAGTCAAATTAATCAAGACTCCATAC
CAAGAACACTCTGCCAAATTATCCGAAAAACAACAATAAATCA
Length of 3' end seq. 693
Connected seq. ID AFO729P
Connected seq. 
>AFO729P.Seq
GACAATCCATTATTATTTTTATATATAAGTAATGTTTTGATTAAAAATATAATTATATCC
ATTTTTTTATTTCAATTTTTTTATTATTTTTTTTTTTGGTTTTNATTTTTTTTTTTTTAN
TTTTTTANTTTTTTTTTNGGNTAAAAAAAAAA----------GTCCACAAAGAGGTGGTC
AAGGTGAAACCAAATCATGGACTCCAGTCACCAAATTAGGTCGTCTCGTTGCTCTTGGTT
TAGTCGAGAATATCCACGAAATCTATCTTTTCTCTTTACCAATCAAGGAGTACCAAATCA
TTGATAAATTCCTTAACCTCAAGGATGAAGTTATGAAGATTGTTCCAGTTCAAAAGCAAA
CTCGTGCTGGTCAACGTACCCGTTTCAAGGCTTTCGTCGTTGTTGGTGATCACAACGGTC
ATGTAGGTCTCGGTGTTAAATGCGCTAAGGAAGTTGCCACCGCCATTTCTGGTGCTATCG
TTGCTGCCAAATTATCAGTCATCCCAGTTCGTCGTGGTTATTGGGGTAACAAACTCGGTG
CTCCACACACTGTCCCAACCAAAGTCACTGGTAAATGCGGTTCCGTCGCTGTACGTCTCG
TTCCAGCCCCAAGAGGTACTGGTATCGTTGCTGCTCGTGTTCCAAAGAAATTACTCCAAT
ATGCTGGTGTTGATGATGTTTACACATCTTCACGTGGTAAGACCAGAACCATGGGTAACT
TTGTCATGGCCACTTTCTTCGCCATCACCAAGACCTTTGCTTACTTAACCCCAGATCTTT
GGAGAGAAGTCAAATTAATCAAGACTCCATACCAAGAACACTCTGCCAAATTATCCGAAA
AACAACAATAAATCA
Length of connected seq. 845
Full length Seq ID -
Full length Seq. -
Length of full length seq. -