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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO256
Library VH
(Link to library)
Clone ID VHO256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11309-1
Original site URL
Representative seq. ID VHO256P
(Link to Original site)
Representative DNA sequence 
>VHO256 (VHO256Q) /CSM/VH/VHO2-C/VHO256Q.Seq.d/
TAAAAGATGATATATACAGATGCTGGAGTTGATGAAGATAAAGCAACAATAAGACCAAAT
CAAACACATCAATTTCAAAAGGTACAAGATGAAGCAAAAGAATGGACAAAAAATAAATTT
TTAGAATTTTTAAATAATTTTAAATTAAAAAAAAAAATTGATAAAAATAATAATAATAAT
AATAATGAAGATAATGAAGATAATAATGAAAATGAAAATGAATATGACGAAAATGGTATT
AAAAAAGAGAAAATAGATAAAAGTTATTATCGTCAACAAGTTGAAAGAATGATAAAAAAT
GATAAATCAAGTTTATATATAGATTTCTTACATTTANAGAAATTTGATAAAGGTTTAGGA
AAAGCATTATTAATGGAATATTTTAXXXXXXXXXXAAAACTATATAAAGTATACAAAATT
CATTTGTCCAACCATTCCAGATGAAAGTGTACAACTATTGGTGGGACATCACGATAGATT
ACGTCAAATGGATACCAGTGGTTCAAAAACTCCAGCATACAGAATCACAGTTAGACAATT
GGAATCATTAGTTCGTTTATCGGAATCATTGGCTCGTTTACATTTAGATACAAAAGTTTT
ACCAAAATATGTAAATGAAGCTGCAAGATTATTAGAGAAATCAATAGTTCATGTTGAAAC
AAATGATGTAATTTTAGGTGATGATGATGATGATTTAGTCAAAAATGTTGAAAATGATAA
CGATAACCATGCTGAAGAAGATGGTGATGATGGTATTGGCAAATTAACTATGAATTTTTC
AAAATATTCACAATTATCAAAATTATTAGTTTTACAAATTAAACAATCAGGAAAAGAGAA
ATCTGGTATAAAACAAATTGATCTTATCGATTGGTACATTAAAGATCAATTGGAGTCTGG
CATAATCACTGATGATGAAGTTACAAAAGAAACTAAAATCACTAAAATGGTTATCAATAA
AATGATAAACAAAGACAATTCTCTTGTTGTATTAGTACCAAATCAATATCCNATCATAGA
ATATTATTGACATCCAAATTATTCATTNATAAATAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
*KMIYTDAGVDEDKATIRPNQTHQFQKVQDEAKEWTKNKFLEFLNNFKLKKKIDKNNNNN
NNEDNEDNNENENEYDENGIKKEKIDKSYYRQQVERMIKNDKSSLYIDFLHLXKFDKGLG
KALLMEYF---

---NYIKYTKFICPTIPDESVQLLVGHHDRLRQMDTSGSKTPAYRITVRQLESLVRLSES
LARLHLDTKVLPKYVNEAARLLEKSIVHVETNDVILGDDDDDLVKNVENDNDNHAEEDGD
DGIGKLTMNFSKYSQLSKLLVLQIKQSGKEKSGIKQIDLIDWYIKDQLESGIITDDEVTK
ETKITKMVINKMINKDNSLVVLVPNQYPIIEYY*hpnysxink
Translated Amino Acid sequence (All Frames) 
Frame A:
*KMIYTDAGVDEDKATIRPNQTHQFQKVQDEAKEWTKNKFLEFLNNFKLKKKIDKNNNNN
NNEDNEDNNENENEYDENGIKKEKIDKSYYRQQVERMIKNDKSSLYIDFLHLXKFDKGLG
KALLMEYF---

---kti*siqnsfvqpfqmkvynywwditidyvkwipvvqklqhtesqldnwnh*fvyrn
hwlvyi*iqkfyqnm*mklqdy*rnq*fmlkqmm*f*vmmmmi*skmlkmititmlkkmv
mmvlan*l*ifqnihnyqny*fyklnnqekrnlv*nklilsigtlkinwsla*slmmklq
kklkslkwlsik**tktillly*yqinixs*niidiqiihx*in

Frame B:
kr*yiqmlelmkikqq*dqikhinfkrykmkqkngqkinf*nf*iiln*kkklikiiiii
imkimkiimkmkmnmtkmvlkkrk*ikviivnklke**kminqvyi*isyixrnlikv*e
khy*wnil---

---klykvykihlsnhsr*kcttiggtsr*itsngyqwfknssiqnhs*tigiissfigi
igsftfryksftkick*sckiireinssc*nk*cnfr*****fsqkc*k**r*pc*rrw*
*wywqinyeffkiftiikiisftn*tirkreiwyktn*syrlvh*rsigvwhnh***syk
rn*nh*ngyq*ndkqrqfsccistksisxhrilltsklfixk*

Frame C:
kddiyrcws**r*snnktksntsiskgtr*skrmdkk*ifrifk*f*ikkkn**k*****
**r**r***k*k*i*rkwy*krenr*kllssts*kndkk**ikfiyrfltfxei**rfrk
siingif---

---NYIKYTKFICPTIPDESVQLLVGHHDRLRQMDTSGSKTPAYRITVRQLESLVRLSES
LARLHLDTKVLPKYVNEAARLLEKSIVHVETNDVILGDDDDDLVKNVENDNDNHAEEDGD
DGIGKLTMNFSKYSQLSKLLVLQIKQSGKEKSGIKQIDLIDWYIKDQLESGIITDDEVTK
ETKITKMVINKMINKDNSLVVLVPNQYPIIEYY*hpnysxink
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO256 (VHO256Q) /CSM/VH/VHO2-C/VHO256Q.Seq.d/                       1520   0.0
AHE675 (AHE675Q) /CSM/AH/AHE6-D/AHE675Q.Seq.d/                       1263   0.0
SHJ521 (SHJ521Q) /CSM/SH/SHJ5-A/SHJ521Q.Seq.d/                       1243   0.0
SHG102 (SHG102Q) /CSM/SH/SHG1-A/SHG102Q.Seq.d/                       1239   0.0
AHL565 (AHL565Q) /CSM/AH/AHL5-C/AHL565Q.Seq.d/                       1237   0.0
AHJ750 (AHJ750Q) /CSM/AH/AHJ7-C/AHJ750Q.Seq.d/                       1181   0.0
VSI736 (VSI736Q) /CSM/VS/VSI7-B/VSI736Q.Seq.d/                        143   6e-33
SSI745 (SSI745Q) /CSM/SS/SSI7-B/SSI745Q.Seq.d/                         42   0.017
AFJ508 (AFJ508Q) /CSM/AF/AFJ5-A/AFJ508Q.Seq.d/                         42   0.017
VSF451 (VSF451Q) /CSM/VS/VSF4-C/VSF451Q.Seq.d/                         40   0.068
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445516) Dictyostelium discoideum cDNA clone:ddv59o14, 3' ...  1302   0.0    1
(BJ350716) Dictyostelium discoideum cDNA clone:dda40e20, 3' ...  1247   0.0    1
(BJ407613) Dictyostelium discoideum cDNA clone:dds43i05, 3' ...  1241   0.0    1
(BJ354704) Dictyostelium discoideum cDNA clone:dda54a17, 3' ...  1237   0.0    1
(AC117075) Dictyostelium discoideum chromosome 2 map 5201047...  1213   0.0    3
(BJ406658) Dictyostelium discoideum cDNA clone:dds36c01, 3' ...  1205   0.0    3
(BJ353525) Dictyostelium discoideum cDNA clone:dda50d13, 3' ...  1070   0.0    2
(BJ426635) Dictyostelium discoideum cDNA clone:ddv59o14, 5' ...   238   e-114  2
(BJ394651) Dictyostelium discoideum cDNA clone:dds36c01, 5' ...   238   e-114  2
(BJ396560) Dictyostelium discoideum cDNA clone:dds43i05, 5' ...   238   e-114  2
dna update 2008.12.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q61J08) RecName: Full=DNA replication licensing factor mcm-6;        120   6e-26
Z22181_1(Z22181|pid:none) Caenorhabditis elegans Cosmid ZK632, c...   120   1e-25
(P34647) RecName: Full=DNA replication licensing factor mcm-6;  ...   120   1e-25
S40933(S40933)probable replication licensing factor MCM6 - Caeno...   120   1e-25
AJ720016_1(AJ720016|pid:none) Gallus gallus mRNA for hypothetica...   120   1e-25
AJ720172_1(AJ720172|pid:none) Gallus gallus mRNA for hypothetica...   120   1e-25
(Q2KIZ8) RecName: Full=DNA replication licensing factor MCM6;  &...   117   7e-25
AF031139_1(AF031139|pid:none) Xenopus laevis zygotic DNA replica...   117   7e-25
(Q62724) RecName: Full=DNA replication licensing factor MCM6; Al...   115   2e-24
(Q7ZY18) RecName: Full=Zygotic DNA replication licensing factor ...   115   2e-24
protein update 2009. 5.18
PSORT 

 psg: 0.75  gvh: 0.10  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHO256 is nuc
5' end seq. ID VHO256F
5' end seq. 
>VHO256F.Seq
TAAAAGATGATATATACAGATGCTGGAGTTGATGAAGATAAAGCAACAATAAGACCAAAT
CAAACACATCAATTTCAAAAGGTACAAGATGAAGCAAAAGAATGGACAAAAAATAAATTT
TTAGAATTTTTAAATAATTTTAAATTAAAAAAAAAAATTGATAAAAATAATAATAATAAT
AATAATGAAGATAATGAAGATAATAATGAAAATGAAAATGAATATGACGAAAATGGTATT
AAAAAAGAGAAAATAGATAAAAGTTATTATCGTCAACAAGTTGAAAGAATGATAAAAAAT
GATAAATCAAGTTTATATATAGATTTCTTACATTTANAGAAATTTGATAAAGGTTTAGGA
AAAGCATTATTAATGGAATATTTTANNNNNNNNNN
Length of 5' end seq. 395
3' end seq. ID VHO256Z
3' end seq. 
>VHO256Z.Seq
NNNNNNNNNNAAAACTATATAAAGTATACAAAATTCATTTGTCCAACCATTCCAGATGAA
AGTGTACAACTATTGGTGGGACATCACGATAGATTACGTCAAATGGATACCAGTGGTTCA
AAAACTCCAGCATACAGAATCACAGTTAGACAATTGGAATCATTAGTTCGTTTATCGGAA
TCATTGGCTCGTTTACATTTAGATACAAAAGTTTTACCAAAATATGTAAATGAAGCTGCA
AGATTATTAGAGAAATCAATAGTTCATGTTGAAACAAATGATGTAATTTTAGGTGATGAT
GATGATGATTTAGTCAAAAATGTTGAAAATGATAACGATAACCATGCTGAAGAAGATGGT
GATGATGGTATTGGCAAATTAACTATGAATTTTTCAAAATATTCACAATTATCAAAATTA
TTAGTTTTACAAATTAAACAATCAGGAAAAGAGAAATCTGGTATAAAACAAATTGATCTT
ATCGATTGGTACATTAAAGATCAATTGGAGTCTGGCATAATCACTGATGATGAAGTTACA
AAAGAAACTAAAATCACTAAAATGGTTATCAATAAAATGATAAACAAAGACAATTCTCTT
GTTGTATTAGTACCAAATCAATATCCNATCATAGAATATTATTGACATCCAAATTATTCA
TTNATAAATAAAT
Length of 3' end seq. 673
Connected seq. ID VHO256P
Connected seq. 
>VHO256P.Seq
TAAAAGATGATATATACAGATGCTGGAGTTGATGAAGATAAAGCAACAATAAGACCAAAT
CAAACACATCAATTTCAAAAGGTACAAGATGAAGCAAAAGAATGGACAAAAAATAAATTT
TTAGAATTTTTAAATAATTTTAAATTAAAAAAAAAAATTGATAAAAATAATAATAATAAT
AATAATGAAGATAATGAAGATAATAATGAAAATGAAAATGAATATGACGAAAATGGTATT
AAAAAAGAGAAAATAGATAAAAGTTATTATCGTCAACAAGTTGAAAGAATGATAAAAAAT
GATAAATCAAGTTTATATATAGATTTCTTACATTTANAGAAATTTGATAAAGGTTTAGGA
AAAGCATTATTAATGGAATATTTTA----------AAAACTATATAAAGTATACAAAATT
CATTTGTCCAACCATTCCAGATGAAAGTGTACAACTATTGGTGGGACATCACGATAGATT
ACGTCAAATGGATACCAGTGGTTCAAAAACTCCAGCATACAGAATCACAGTTAGACAATT
GGAATCATTAGTTCGTTTATCGGAATCATTGGCTCGTTTACATTTAGATACAAAAGTTTT
ACCAAAATATGTAAATGAAGCTGCAAGATTATTAGAGAAATCAATAGTTCATGTTGAAAC
AAATGATGTAATTTTAGGTGATGATGATGATGATTTAGTCAAAAATGTTGAAAATGATAA
CGATAACCATGCTGAAGAAGATGGTGATGATGGTATTGGCAAATTAACTATGAATTTTTC
AAAATATTCACAATTATCAAAATTATTAGTTTTACAAATTAAACAATCAGGAAAAGAGAA
ATCTGGTATAAAACAAATTGATCTTATCGATTGGTACATTAAAGATCAATTGGAGTCTGG
CATAATCACTGATGATGAAGTTACAAAAGAAACTAAAATCACTAAAATGGTTATCAATAA
AATGATAAACAAAGACAATTCTCTTGTTGTATTAGTACCAAATCAATATCCNATCATAGA
ATATTATTGACATCCAAATTATTCATTNATAAATAAAT
Length of connected seq. 1048
Full length Seq ID -
Full length Seq. -
Length of full length seq. -