>AFO717 (AFO717Q) /CSM/AF/AFO7-A/AFO717Q.Seq.d/
ATTGATGTTTTGGTTTTTGTTCAATATTGCGAGACGAAATCATCACACTTATATATATAA
AATAAAAAAATGTTAGGATTAACTGATATGTTAAAAGATGGTGCAAAGCATTTTGCAGGC
AAAGATGAAGCAATCTTAAGAAATATTGATGCAACCAAACAATTATCAGAGATTACAAGA
ACATCATTAGGTCCAAATGGTATGAATAAAATGATCATCAATCATTTAGAGAAATTATTT
GTTACAAATGATGCCGCAACCATCATTAGAGAATTAGATGTTATTCATCCAGCTGCAAAA
ATGTTGGTTATGGCTGCTCAAATGCAAGAACAAGAAATGGGAGATGGTACAAATTATGTT
GTTACATTAACAGGTGAATTTTTACAAAAAGCAGCAACTTTATTAGAGATGGGACTTCAC
CCAAGTGAAATTATAACTGGTTTTGAAAAAGCAGGTGCTAAACTTCAAGAGATTATTGAA
TCAATGATTGTATACAATTTAAAGGATATTACAGATAAGAAGGAGGTTACAAAATGTTTG
AAATCAGCCATCGCAAGTAAACAATATGGATATGAGGAATTTTTATCAGAGATTATCACC
AATGCATGTCTTCAAGTTTTACCAAAGAAAGCAGTTAATTTCAATATCGATAATGTTAGA
GTCACCAAGATTXXXXXXXXXXGCTTTACATTACATTGAAAGATTCAAAATCATGTTGGT
TCGTATTCAATCTAAATTCCAATTACGTCGTGTTTGTAAAGCAATCGGTGCAACTCCATT
AGTTAAATTGGGTGCCCCTATCCCTGAGGAATTGGGTTACTGTGCTGAAGTACTCGTTGA
AGAGATCGGTTCAACCAAATGTTGTATTTTCCGTCAAAATAAAGAAGAAAGTGAAATCTC
CACCATTGTAGTTCGTGGTTCAACCAATAACATTCTCGATGATATTGAACGTGCCATCGA
TGATGGTGTCAACGTTTTCAAAGGTATGTGTAAAGATGGTAGATTCTTAGCTGGTGCTGG
TGCTTTTGAAATTGAAGCCTCACGTAAACTCCAAGCTTTTGCCGATGCTACACCAGGTCT
CTCTCAATATTCAATTCGTCAATACGCTGAAGCTTTCGAAATCATTCCAAGAACTCTTGC
TGAAACTAGTGGTCACGACTCCTCAAAAGCAATCTCAAATATTTATGCAGCTCATACCAA
AGGTAATACCAATTATGGTTTAGATATTGAATCTGGTCAACCAAAATCTGTACTCGAAAT
GGATGTTTTAGACGCTTTTGCTTCAAAACTTTTCGCAATTAAATTAGCTACAAACACTGC
TAACACCGTTTTAAGAGTCGATCAAATCATTATGTCTAAACCAGCGGGGTCCAAAACCAC
CAAAAAGGGTCCAACCGA

idvlvfvqycetksshlyi*NKKMLGLTDMLKDGAKHFAGKDEAILRNIDATKQLSEITR
TSLGPNGMNKMIINHLEKLFVTNDAATIIRELDVIHPAAKMLVMAAQMQEQEMGDGTNYV
VTLTGEFLQKAATLLEMGLHPSEIITGFEKAGAKLQEIIESMIVYNLKDITDKKEVTKCL
KSAIASKQYGYEEFLSEIITNACLQVLPKKAVNFNIDNVRVTKI---

---ALHYIERFKIMLVRIQSKFQLRRVCKAIGATPLVKLGAPIPEELGYCAEVLVEEIGS
TKCCIFRQNKEESEISTIVVRGSTNNILDDIERAIDDGVNVFKGMCKDGRFLAGAGAFEI
EASRKLQAFADATPGLSQYSIRQYAEAFEIIPRTLAETSGHDSSKAISNIYAAHTKGNTN
YGLDIESGQPKSVLEMDVLDAFASKLFAIKLATNTANTVLRVDQIIMSKPAGSKTTKKGP
T

Frame A:
idvlvfvqycetksshlyi*NKKMLGLTDMLKDGAKHFAGKDEAILRNIDATKQLSEITR
TSLGPNGMNKMIINHLEKLFVTNDAATIIRELDVIHPAAKMLVMAAQMQEQEMGDGTNYV
VTLTGEFLQKAATLLEMGLHPSEIITGFEKAGAKLQEIIESMIVYNLKDITDKKEVTKCL
KSAIASKQYGYEEFLSEIITNACLQVLPKKAVNFNIDNVRVTKI---

---ALHYIERFKIMLVRIQSKFQLRRVCKAIGATPLVKLGAPIPEELGYCAEVLVEEIGS
TKCCIFRQNKEESEISTIVVRGSTNNILDDIERAIDDGVNVFKGMCKDGRFLAGAGAFEI
EASRKLQAFADATPGLSQYSIRQYAEAFEIIPRTLAETSGHDSSKAISNIYAAHTKGNTN
YGLDIESGQPKSVLEMDVLDAFASKLFAIKLATNTANTVLRVDQIIMSKPAGSKTTKKGP
T

Frame B:
lmfwflfniarrnhhtyiykikkc*d*lic*kmvqsilqakmkqs*eilmqpnnyqrlqe
hh*vqmv*ik*ssii*rnyllqmmpqpslen*mlfiqlqkcwlwllkcknkkwemvqiml
lh*qvnfykkqqly*rwdftqvkl*lvlkkqvlnfkrllnq*lyti*rilqirrrlqnv*
nqpsqvnnmdmrnfyqrlspmhvfkfyqrkqlisisimlespr---

---lyitlkdskscwfvfnlnsnyvvfvkqsvqlh*lnwvplslrnwvtvlkyslkrsvq
pnvvfsvkikkkvksppl*fvvqpitfsmilnvpsmmvstfskvcvkmvds*lvlvllkl
kphvnskllpmlhqvslniqfvntlklsksfqelllklvvttpqkqsqifmqlipkvipi
mv*ilnlvnqnlyskwmf*tlllqnfsqln*lqtlltpf*esikslclnqrgpkppkrvq
p

Frame C:
*cfgfcsilrdeiitliyik*knvrin*yvkrwckafcrqr*snlkky*cnqtiirdykn
iirskwye*ndhqsfreiicyk*crnhh*rircyssscknvgygcsnartrngrwyklcc
yinr*iftkssnfirdgtspk*nynwf*ksrc*tsrdy*indciqfkgyyr*eggykmfe
ishrk*tiwi*gifirdyhqcmsssftkess*fqyr*c*shqd---

---ftlh*kiqnhvgsysi*ipitscl*snrcnsis*igcpyp*gigllc*str*rdrfn
qmlyfpsk*rrk*nlhhcsswfnq*hsr*y*tchr*wcqrfqryv*rw*ilswcwcf*n*
slt*tpsfcrcytrslsifnssir*sfrnhsknsc*n*wsrllksnlkylcssyqr*yql
wfry*iwstkictrngcfrrfcfktfrn*isykhc*hrfksrsnhyv*tsgvqnhqkgsn
r

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO717 (AFO717Q) /CSM/AF/AFO7-A/AFO717Q.Seq.d/                       2670   0.0
VFA682 (VFA682Q) /CSM/VF/VFA6-D/VFA682Q.Seq.d/                       1411   0.0
VFF643 (VFF643Q) /CSM/VF/VFF6-B/VFF643Q.Seq.d/                       1402   0.0
AFC664 (AFC664Q) /CSM/AF/AFC6-C/AFC664Q.Seq.d/                       1398   0.0
CHE274 (CHE274Q) /CSM/CH/CHE2-D/CHE274Q.Seq.d/                       1396   0.0
AFE837 (AFE837Q) /CSM/AF/AFE8-B/AFE837Q.Seq.d/                       1394   0.0
VFC658 (VFC658Q) /CSM/VF/VFC6-C/VFC658Q.Seq.d/                       1384   0.0
SFD263 (SFD263Q) /CSM/SF/SFD2-C/SFD263Q.Seq.d/                       1384   0.0
SFB643 (SFB643Q) /CSM/SF/SFB6-B/SFB643Q.Seq.d/                       1384   0.0
CFF166 (CFF166Q) /CSM/CF/CFF1-C/CFF166Q.Seq.d/                       1382   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1346  0.0  6
CB554964|CB554964.1 MMSP0032_C03 MMSP Macaca mulatta cDNA, mRNA sequence.    56  2e-11  3
CB552391|CB552391.1 MMSP0023_B10 MMSP Macaca mulatta cDNA, mRNA sequence.    56  2e-11  3
CB311708|CB311708.1 AGENCOURT_11834823 NICHD_Rh_Ov1 Macaca mulatta cDNA clone IMAGE:6891834 5', mRNA sequence.    56  3e-11  3
AF442548|AF442548.1 Physarum polycephalum CCT chaperonin theta subunit mRNA, complete cds.    64  6e-07  2
BY239869|BY239869.1 Mus musculus visual cortex cDNA, RIKEN full-length enriched library, clone:K230014M17, 5' end partial sequence.    46  7e-07  2
BY249170|BY249170.1 Mus musculus visual cortex cDNA, RIKEN full-length enriched library, clone:K230322G01, 5' end partial sequence.    46  7e-07  2
BB610572|BB610572.1 Mus musculus adult male tongue cDNA, RIKEN full-length enriched library, clone:2310021D01, 5' end partial sequence.    46  1e-06  2
CB206821|CB206821.1 AGENCOURT_11373964 NICHD_XGC_Tad1 Xenopus laevis cDNA clone IMAGE:6878875 5', mRNA sequence.    66  1e-06  1
AL033864|AL033864.1 H.sapiens flow-sorted chromosome 1 HindIII fragment, SC1pE51B03.    48  2e-06  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116979_86(AC116979|pid:none) Dictyostelium discoideum chromoso...   452   e-125
AF442548_1(AF442548|pid:none) Physarum polycephalum CCT chaperon...   323   1e-86
(Q3ZCI9) RecName: Full=T-complex protein 1 subunit theta;       ...   261   4e-68
AK145918_1(AK145918|pid:none) Mus musculus 14 days pregnant adul...   261   5e-68
(P42932) RecName: Full=T-complex protein 1 subunit theta;       ...   260   9e-68
AB022161_1(AB022161|pid:none) Mus musculus Cctq gene for chapero...   260   9e-68
AK222490_1(AK222490|pid:none) Homo sapiens mRNA for chaperonin c...   259   2e-67
D42052_1(D42052|pid:none) Homo sapiens cosmid Q7A10 (D21S246) in...   259   2e-67
BC050492_1(BC050492|pid:none) Danio rerio chaperonin containing ...   257   8e-67
AY823273_1(AY823273|pid:none) Notothenia coriiceps TCP1-theta mR...   251   4e-65

 psg: 0.54  gvh: 0.24  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 44.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFO717 is nuc

>AFO717F.Seq
ATTGATGTTTTGGTTTTTGTTCAATATTGCGAGACGAAATCATCACACTTATATATATAA
AATAAAAAAATGTTAGGATTAACTGATATGTTAAAAGATGGTGCAAAGCATTTTGCAGGC
AAAGATGAAGCAATCTTAAGAAATATTGATGCAACCAAACAATTATCAGAGATTACAAGA
ACATCATTAGGTCCAAATGGTATGAATAAAATGATCATCAATCATTTAGAGAAATTATTT
GTTACAAATGATGCCGCAACCATCATTAGAGAATTAGATGTTATTCATCCAGCTGCAAAA
ATGTTGGTTATGGCTGCTCAAATGCAAGAACAAGAAATGGGAGATGGTACAAATTATGTT
GTTACATTAACAGGTGAATTTTTACAAAAAGCAGCAACTTTATTAGAGATGGGACTTCAC
CCAAGTGAAATTATAACTGGTTTTGAAAAAGCAGGTGCTAAACTTCAAGAGATTATTGAA
TCAATGATTGTATACAATTTAAAGGATATTACAGATAAGAAGGAGGTTACAAAATGTTTG
AAATCAGCCATCGCAAGTAAACAATATGGATATGAGGAATTTTTATCAGAGATTATCACC
AATGCATGTCTTCAAGTTTTACCAAAGAAAGCAGTTAATTTCAATATCGATAATGTTAGA
GTCACCAAGATT----------

>AFO717Z.Seq
----------GCTTTACATTACATTGAAAGATTCAAAATCATGTTGGTTCGTATTCAATC
TAAATTCCAATTACGTCGTGTTTGTAAAGCAATCGGTGCAACTCCATTAGTTAAATTGGG
TGCCCCTATCCCTGAGGAATTGGGTTACTGTGCTGAAGTACTCGTTGAAGAGATCGGTTC
AACCAAATGTTGTATTTTCCGTCAAAATAAAGAAGAAAGTGAAATCTCCACCATTGTAGT
TCGTGGTTCAACCAATAACATTCTCGATGATATTGAACGTGCCATCGATGATGGTGTCAA
CGTTTTCAAAGGTATGTGTAAAGATGGTAGATTCTTAGCTGGTGCTGGTGCTTTTGAAAT
TGAAGCCTCACGTAAACTCCAAGCTTTTGCCGATGCTACACCAGGTCTCTCTCAATATTC
AATTCGTCAATACGCTGAAGCTTTCGAAATCATTCCAAGAACTCTTGCTGAAACTAGTGG
TCACGACTCCTCAAAAGCAATCTCAAATATTTATGCAGCTCATACCAAAGGTAATACCAA
TTATGGTTTAGATATTGAATCTGGTCAACCAAAATCTGTACTCGAAATGGATGTTTTAGA
CGCTTTTGCTTCAAAACTTTTCGCAATTAAATTAGCTACAAACACTGCTAACACCGTTTT
AAGAGTCGATCAAATCATTATGTCTAAACCAGCGGGGTCCAAAACCACCAAAAAGGGTCC
AACCGA

>AFO717P.Seq
ATTGATGTTTTGGTTTTTGTTCAATATTGCGAGACGAAATCATCACACTTATATATATAA
AATAAAAAAATGTTAGGATTAACTGATATGTTAAAAGATGGTGCAAAGCATTTTGCAGGC
AAAGATGAAGCAATCTTAAGAAATATTGATGCAACCAAACAATTATCAGAGATTACAAGA
ACATCATTAGGTCCAAATGGTATGAATAAAATGATCATCAATCATTTAGAGAAATTATTT
GTTACAAATGATGCCGCAACCATCATTAGAGAATTAGATGTTATTCATCCAGCTGCAAAA
ATGTTGGTTATGGCTGCTCAAATGCAAGAACAAGAAATGGGAGATGGTACAAATTATGTT
GTTACATTAACAGGTGAATTTTTACAAAAAGCAGCAACTTTATTAGAGATGGGACTTCAC
CCAAGTGAAATTATAACTGGTTTTGAAAAAGCAGGTGCTAAACTTCAAGAGATTATTGAA
TCAATGATTGTATACAATTTAAAGGATATTACAGATAAGAAGGAGGTTACAAAATGTTTG
AAATCAGCCATCGCAAGTAAACAATATGGATATGAGGAATTTTTATCAGAGATTATCACC
AATGCATGTCTTCAAGTTTTACCAAAGAAAGCAGTTAATTTCAATATCGATAATGTTAGA
GTCACCAAGATT----------GCTTTACATTACATTGAAAGATTCAAAATCATGTTGGT
TCGTATTCAATCTAAATTCCAATTACGTCGTGTTTGTAAAGCAATCGGTGCAACTCCATT
AGTTAAATTGGGTGCCCCTATCCCTGAGGAATTGGGTTACTGTGCTGAAGTACTCGTTGA
AGAGATCGGTTCAACCAAATGTTGTATTTTCCGTCAAAATAAAGAAGAAAGTGAAATCTC
CACCATTGTAGTTCGTGGTTCAACCAATAACATTCTCGATGATATTGAACGTGCCATCGA
TGATGGTGTCAACGTTTTCAAAGGTATGTGTAAAGATGGTAGATTCTTAGCTGGTGCTGG
TGCTTTTGAAATTGAAGCCTCACGTAAACTCCAAGCTTTTGCCGATGCTACACCAGGTCT
CTCTCAATATTCAATTCGTCAATACGCTGAAGCTTTCGAAATCATTCCAAGAACTCTTGC
TGAAACTAGTGGTCACGACTCCTCAAAAGCAATCTCAAATATTTATGCAGCTCATACCAA
AGGTAATACCAATTATGGTTTAGATATTGAATCTGGTCAACCAAAATCTGTACTCGAAAT
GGATGTTTTAGACGCTTTTGCTTCAAAACTTTTCGCAATTAAATTAGCTACAAACACTGC
TAACACCGTTTTAAGAGTCGATCAAATCATTATGTCTAAACCAGCGGGGTCCAAAACCAC
CAAAAAGGGTCCAACCGA