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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO146
Library VH
(Link to library)
Clone ID VHO146
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14496-1
Original site URL
Representative seq. ID VHO146P
(Link to Original site)
Representative DNA sequence 
>VHO146 (VHO146Q) /CSM/VH/VHO1-B/VHO146Q.Seq.d/
TAAGCATAGAAAATNAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAG
AAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGA
CAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGAAA
CCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGT
CAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAA
AGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGC
TCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTT
AGTTGCTTAAATATTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTTAA
AAAATANAAAAAAAAXXXXXXXXXXAGTCAGTATCACATATTAAAATGCCAACCAGATTC
AGTAAGCATAGAAAATCAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACAC
AGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTT
GACAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGA
AACCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCC
GTCAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAA
AAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAA
GCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATC
TTAGTTGCTTAAATATTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTT
AAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
khrkxrgdvcagygrvgkhrkhpggrgnagglthhrinfdkyhpgyfgklgmrhfhllrn
qyhcptvslekiwtlvpesvrkslaakndgtapvvdvtqkgffkvlghgilptqpiivka
ryfskvaekkikavggacilva*ifytnq*i*IKKVIKFKKXKK---

---SVSHIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPG
YFGKLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKV
LGHGILPTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfk
Translated Amino Acid sequence (All Frames) 
Frame A:
*a*kxkr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsrlfr*iryetfpfikk
piplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqsfrsryppnstnhrqs
slfl*sr*kenqscrwclylsclnilyesininkksn*i*kixkk---

---sqyhilkcqpdsvsienqevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiq
visvn*v*disiy*etntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvssk
f*vtvssqlnqsssklvislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlk

Frame B:
khrkxrgdvcagygrvgkhrkhpggrgnagglthhrinfdkyhpgyfgklgmrhfhllrn
qyhcptvslekiwtlvpesvrkslaakndgtapvvdvtqkgffkvlghgilptqpiivka
ryfskvaekkikavggacilva*ifytnq*i*IKKVIKFKKXKK---

---vsity*nanqiq*a*kikr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsr
lfr*iryetfpfikkpiplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqs
frsryppnstnhrqsslfl*sr*kenqscrwclylsclnilyesininkksn*i*k

Frame C:
sienxevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiqvisvn*v*disiy*et
ntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvsskf*vtvssqlnqssskl
vislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlknxkk---

---SVSHIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPG
YFGKLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKV
LGHGILPTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO146 (VHO146Q) /CSM/VH/VHO1-B/VHO146Q.Seq.d/                        955   0.0
VHG250 (VHG250Q) /CSM/VH/VHG2-C/VHG250Q.Seq.d/                        955   0.0
VFF473 (VFF473Q) /CSM/VF/VFF4-D/VFF473Q.Seq.d/                        955   0.0
VFE287 (VFE287Q) /CSM/VF/VFE2-D/VFE287Q.Seq.d/                        955   0.0
VSJ737 (VSJ737Q) /CSM/VS/VSJ7-B/VSJ737Q.Seq.d/                        948   0.0
VHE734 (VHE734Q) /CSM/VH/VHE7-B/VHE734Q.Seq.d/                        948   0.0
VSI571 (VSI571Q) /CSM/VS/VSI5-C/VSI571Q.Seq.d/                        942   0.0
VSE523 (VSE523Q) /CSM/VS/VSE5-A/VSE523Q.Seq.d/                        942   0.0
VSA537 (VSA537Q) /CSM/VS/VSA5-B/VSA537Q.Seq.d/                        942   0.0
VSH826 (VSH826Q) /CSM/VS/VSH8-B/VSH826Q.Seq.d/                        938   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445414) Dictyostelium discoideum cDNA clone:ddv59k11, 3' ...   957   0.0    1
(BJ431114) Dictyostelium discoideum cDNA clone:ddv9m22, 3' e...   957   0.0    1
(BJ428387) Dictyostelium discoideum cDNA clone:ddv11b20, 3' ...   957   0.0    1
(BJ421687) Dictyostelium discoideum cDNA clone:ddv43c14, 5' ...   957   0.0    1
(BJ412802) Dictyostelium discoideum cDNA clone:ddv9m22, 5' e...   957   0.0    1
(BJ410234) Dictyostelium discoideum cDNA clone:ddv11b20, 5' ...   957   0.0    1
(AU270392) Dictyostelium discoideum vegetative cDNA clone:VS...   950   0.0    1
(BJ420740) Dictyostelium discoideum cDNA clone:ddv40d09, 5' ...   950   0.0    1
(AU268909) Dictyostelium discoideum vegetative cDNA clone:VS...   944   0.0    1
(AU263167) Dictyostelium discoideum vegetative cDNA clone:VS...   944   0.0    1
dna update 2008.12. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P48160) RecName: Full=60S ribosomal protein L27a;  &D50338_1(D5...   313   8e-84
BT046766_1(BT046766|pid:none) Salmo salar clone ssal-eve-548-138...   226   8e-58
AY961477_1(AY961477|pid:none) Phytophthora infestans clone MY-19...   226   1e-57
AF502247_1(AF502247|pid:none) Epinephelus coioides ribosomal pro...   223   1e-56
AJ843085_1(AJ843085|pid:none) Platichthys flesus mRNA for 60S ri...   223   1e-56
(P18445) RecName: Full=60S ribosomal protein L27a;  &R5RTLA(S130...   222   1e-56
AB374966_1(AB374966|pid:none) Solea senegalensis RPL27a mRNA for...   221   2e-56
(P46776) RecName: Full=60S ribosomal protein L27a;  &(Q5REY2) Re...   221   2e-56
AJ307670_3(AJ307670|pid:none) Mus musculus St5 gene, L27a gene a...   221   2e-56
AB172987_1(AB172987|pid:none) Macaca fascicularis brain cDNA clo...   220   5e-56
protein update 2009. 7.18
PSORT 

 psg: 0.65  gvh: 0.18  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.48  mip: 0.14
 nuc: 0.17  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial

>> prediction for VHO146 is cyt
5' end seq. ID VHO146F
5' end seq. 
>VHO146F.Seq
TAAGCATAGAAAATNAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAG
AAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGA
CAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGAAA
CCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGT
CAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAA
AGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGC
TCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTT
AGTTGCTTAAATATTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTTAA
AAAATANAAAAAAAANNNNNNNNNN
Length of 5' end seq. 505
3' end seq. ID VHO146Z
3' end seq. 
>VHO146Z.Seq
NNNNNNNNNNAGTCAGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAA
TCAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGT
GGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCA
GGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGT
CCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTA
GCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAA
GTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCT
AAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATA
TTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTTAAAAA
Length of 3' end seq. 530
Connected seq. ID VHO146P
Connected seq. 
>VHO146P.Seq
TAAGCATAGAAAATNAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAG
AAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGA
CAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGAAA
CCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGT
CAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAA
AGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGC
TCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTT
AGTTGCTTAAATATTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTTAA
AAAATANAAAAAAAA----------AGTCAGTATCACATATTAAAATGCCAACCAGATTC
AGTAAGCATAGAAAATCAAGAGGTGACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACAC
AGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACTCATCACAGAATTAACTTT
GACAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGACATTTCCATTTATTAAGA
AACCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGGACTTTAGTCCCAGAATCC
GTCAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCAGTTGTTGATGTCACCCAA
AAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACTCAACCAATCATCGTCAAA
GCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATC
TTAGTTGCTTAAATATTTTATACGAATCAATAAATATAAATAAAAAAAGTAATTAAATTT
AAAAA
Length of connected seq. 1015
Full length Seq ID -
Full length Seq. -
Length of full length seq. -