>AFO715 (AFO715Q) /CSM/AF/AFO7-A/AFO715Q.Seq.d/
GGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGANAATTTCCA
TAATTCAGTTTNTGAACATGACGTAACTNTTGTTATGTTTTATGCCCCATGGNGCGGNCA
TTGNAAAACTTTAAAACCATTXXXXXXXXXXTAACACTCCAAGTTCTCGAAGATGTAAAG
AAAATCGCTACCTCACCAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATT
CCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGA
TTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATAC
CGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCA
ACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAA
GTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCA
TTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATC
TGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAG
AGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGA
AGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGA
ATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCA
AACA

DHEHDHDESFVKILXSXNFHNSVXEHDVTXVMFYAPWXGHXKTLKP---

---NTPSSRRCKENRYLTKKVMPFSVGSI*rnshnkqliwvslvklfqpslliqlpiklv
tismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqr
mfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscs
sklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmimxi*krffck

Frame A:
gs*t*s**iic*nlxf*xfp*fsx*t*rnxcyvlcpmxrxlxnfkti---

---*hskfskm*rkslphqkgnavfcwvdmkkfpqqathmglsgkvvpaisvdsvankar
ynfdeketfsfdtvskwiqdviggkvspfvksqpepesndapvkvavgttfkklvldspk
dvlvefyapwcghcknlapiydklgeylkdvesvsivkidadsndvpsdieirgyptiml
fkaddkenpisyegqrndhmnfvefiqdnaaiefklpssqtddnvnlkkillqt

Frame B:
DHEHDHDESFVKILXSXNFHNSVXEHDVTXVMFYAPWXGHXKTLKP---

---NTPSSRRCKENRYLTKKVMPFSVGSI*rnshnkqliwvslvklfqpslliqlpiklv
tismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqr
mfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscs
sklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmimxi*krffck

Frame C:
imnmimmnhllksxilxisiiqfxnmt*lllcfmphgaxixkl*nh---

---tlqvledvkkiatspkr*crfllgryeeipttsnsygslw*scsshlc*fscq*ssl
qfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkg
cfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhal
qs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn***cxskkdssan

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO715 (AFO715Q) /CSM/AF/AFO7-A/AFO715Q.Seq.d/                       1566   0.0
AFM226 (AFM226Q) /CSM/AF/AFM2-B/AFM226Q.Seq.d/                       1352   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1342   0.0
AFO630 (AFO630Q) /CSM/AF/AFO6-B/AFO630Q.Seq.d/                       1342   0.0
AFN674 (AFN674Q) /CSM/AF/AFN6-D/AFN674Q.Seq.d/                       1342   0.0
AFN461 (AFN461Q) /CSM/AF/AFN4-C/AFN461Q.Seq.d/                       1342   0.0
AFO713 (AFO713Q) /CSM/AF/AFO7-A/AFO713Q.Seq.d/                       1334   0.0
AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1334   0.0
AFG696 (AFG696Q) /CSM/AF/AFG6-D/AFG696Q.Seq.d/                       1334   0.0
AFN781 (AFN781Q) /CSM/AF/AFN7-D/AFN781Q.Seq.d/                       1328   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  3e-09  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  5e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  3e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  1e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  5e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  5e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  5e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   169   1e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   165   2e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   164   4e-39
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   163   7e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   159   1e-37
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   155   1e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   3e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   4e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   4e-35

 psg: 0.75  gvh: 0.37  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.08  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFO715 is nuc

>AFO715F.Seq
GGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGANAATTTCCA
TAATTCAGTTTNTGAACATGACGTAACTNTTGTTATGTTTTATGCCCCATGGNGCGGNCA
TTGNAAAACTTTAAAACCATT----------

>AFO715Z.Seq
----------TAACACTCCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACCAAAA
AGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATAT
GGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCG
TTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGA
TGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGA
TGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAA
GGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAAT
CTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGA
TGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCT
CTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATAT
GAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCA
AACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCAAACA

>AFO715P.Seq
GGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGANAATTTCCA
TAATTCAGTTTNTGAACATGACGTAACTNTTGTTATGTTTTATGCCCCATGGNGCGGNCA
TTGNAAAACTTTAAAACCATT----------TAACACTCCAAGTTCTCGAAGATGTAAAG
AAAATCGCTACCTCACCAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATT
CCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGA
TTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATAC
CGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCA
ACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAA
GTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCA
TTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATC
TGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAG
AGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGA
AGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGA
ATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCA
AACA