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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHO133
Library VH
(Link to library)
Clone ID VHO133
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15717-1
Original site URL
Representative seq. ID VHO133P
(Link to Original site)
Representative DNA sequence 
>VHO133 (VHO133Q) /CSM/VH/VHO1-B/VHO133Q.Seq.d/
ATTTTTTTTTATATTATATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTGGATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAANATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGNGGNGAAAAA
GATTTANAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGNGXX
XXXXXXXXACCAACCACCATCACCAAATGGTCAAAACATTAACGTTATCGTTGATTCAGA
AAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAGATTTAGTCGA
TATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCGATCACATCTCTATGGCTGG
TCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCAACAATATGTTAATTGGTGC
TATCAACTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATCAATTCACTGGTGAAATCGG
TCCAGTCCCAACCGTCGCTCGTGACTACAAGAAACGTGGTGTCAATTGGATCGTCGTTGG
TGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTGCCCTCGAACCACGTCATCT
CGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCCACGAAACCAATCTCAAGAA
ACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATTACGATAAAATCTCTGGTGA
CGATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAGGTAAACAACTCACTCTCAT
TGTTAAATCTGCTAAACAAGGTTCTGAATTTGAAATTAAAGCTAATCACACCATGAATGC
GGTCAAATCGAATGGTTCAAAGCTGGTTCCGCTTTAAATTATATTAAATCAGAAAAAGCC
AAAAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
iffyiiyn*KKNNXKMNSLVKGISKVRSTGSFSTVSMSPLEPNXKLNYEGIDAKLKQFXL
HHNKPLTLAEXIIYGHLXXPSTKVERGITYLKLHPXRVAMQXATAQMAVLQFMSAGLPXT
AVPTTIHCDHLIEAYKGGEKDLXVAKDINKEVYDFLSTX---

---QPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAG
PWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPTVARDYKKRGVNWIVVG
DENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGILPLTFANPSDYDKISGD
DRISIIGLKDLAPGKQLTLIVKSAKQGSEFEIKANHTMNAVKSNGSKLVPL*iilnqkkp
kki
Translated Amino Acid sequence (All Frames) 
Frame A:
iffyiiyn*KKNNXKMNSLVKGISKVRSTGSFSTVSMSPLEPNXKLNYEGIDAKLKQFXL
HHNKPLTLAEXIIYGHLXXPSTKVERGITYLKLHPXRVAMQXATAQMAVLQFMSAGLPXT
AVPTTIHCDHLIEAYKGGEKDLXVAKDINKEVYDFLSTX---

---tnhhhqmvktltlsliqkaadynysnhlphgirki*sickfssrfkvnvppitslwl
vhg*nteviwitfptic*lvlstlktvkptlfsinslvksvqsqpslvttrnvvsigssl
vmktmvkvhlvnmlpsnhvisvvklsslnhllvstkpisrnkvsyh*pllthlitikslv
tivfqslvskilpqvnnslsllnllnkvlnlklklitp*mrsnrmvqswfrfkly*irks
qkk

Frame B:
fffilyiikkkiixk*thslrvfpkldqldlfqqyq*vllnqtxnsttkvlmln*nnsxs
ittnhspllkxsfmvixxihqlklkevslilnyixivllcxmpphkwlyynlcqlvyxkl
qyqqqfivii*lkhikxxkkixklqkisikkftisfqpx---

---pttitkwskh*ryr*frkqqititpticpmg*erfsryasshqgsr*myhrshlygw
smveiqrsfg*hfqqyvnwcyql*kr*sqrcsqsihw*nrsspnrrs*lqetwcqldrrw
**klw*rfis*tccprttssrw*sypr*iicsyprnqsqetryltinlc*pi*lr*nlw*
rsyfnhwsqrscpr*tthshc*ic*trf*i*n*s*shhecgqiewfkagsalnyikseka
kkn

Frame C:
fflyyi*lkkk*x*neltr*gyfqs*inwiffnsiness*tkqxtqlrry*c*iktixtp
sqqtthpc*kxhlwsfxxsin*s*kryhls*itsxsccyaxchrtngcitiyvswftxnc
stnnnsl*sfn*si*rxxkrfxsckryq*rslrfpfnq---

---QPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAG
PWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPTVARDYKKRGVNWIVVG
DENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGILPLTFANPSDYDKISGD
DRISIIGLKDLAPGKQLTLIVKSAKQGSEFEIKANHTMNAVKSNGSKLVPL*iilnqkkp
kki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO133 (VHO133Q) /CSM/VH/VHO1-B/VHO133Q.Seq.d/                       2183   0.0
VHJ814 (VHJ814Q) /CSM/VH/VHJ8-A/VHJ814Q.Seq.d/                       1398   0.0
VHJ504 (VHJ504Q) /CSM/VH/VHJ5-A/VHJ504Q.Seq.d/                       1374   0.0
VHC367 (VHC367Q) /CSM/VH/VHC3-C/VHC367Q.Seq.d/                       1362   0.0
VHJ867 (VHJ867Q) /CSM/VH/VHJ8-C/VHJ867Q.Seq.d/                       1358   0.0
VHF201 (VHF201Q) /CSM/VH/VHF2-A/VHF201Q.Seq.d/                       1322   0.0
CHQ607 (CHQ607Q) /CSM/CH/CHQ6-A/CHQ607Q.Seq.d/                       1312   0.0
VHD386 (VHD386Q) /CSM/VH/VHD3-D/VHD386Q.Seq.d/                       1292   0.0
VHN217 (VHN217Q) /CSM/VH/VHN2-A/VHN217Q.Seq.d/                       1277   0.0
SLA476 (SLA476Q) /CSM/SL/SLA4-D/SLA476Q.Seq.d/                       1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445359) Dictyostelium discoideum cDNA clone:ddv59a09, 3' ...  1411   0.0    1
(BJ442525) Dictyostelium discoideum cDNA clone:ddv50l04, 3' ...  1292   0.0    2
(BJ442499) Dictyostelium discoideum cDNA clone:ddv50g01, 3' ...  1285   0.0    2
(BJ437847) Dictyostelium discoideum cDNA clone:ddv35f17, 3' ...  1273   0.0    3
(BJ438430) Dictyostelium discoideum cDNA clone:ddv37l21, 3' ...  1235   0.0    2
(BJ439599) Dictyostelium discoideum cDNA clone:ddv41a02, 3' ...  1233   0.0    2
(BJ385501) Dictyostelium discoideum cDNA clone:ddc55m02, 3' ...  1233   0.0    2
(BJ442653) Dictyostelium discoideum cDNA clone:ddv50f18, 3' ...  1225   0.0    3
(C25574) Dictyostelium discoideum slug cDNA, clone SLA476.       1078   0.0    2
(AU039889) Dictyostelium discoideum slug cDNA, clone SLG678.     1041   0.0    2
dna update 2008.12. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P49609) RecName: Full=Aconitate hydratase, mitochondrial;      ...   308   2e-84
AB105050_1(AB105050|pid:none) Antheraea yamamai mRNA for hypothe...   308   4e-84
S44831(S44831)F54H12.1 protein - Caenorhabditis elegans               302   1e-83
BC058049_1(BC058049|pid:none) Danio rerio aconitase 2, mitochond...   305   1e-83
L25599_8(L25599|pid:none) Caenorhabditis elegans cosmid F54H12, ...   302   1e-83
L25599_7(L25599|pid:none) Caenorhabditis elegans cosmid F54H12, ...   302   1e-83
AF428141_1(AF428141|pid:none) Gallus gallus aconitase precursor ...   303   3e-83
Z49931_1(Z49931|pid:none) B.taurus mRNA for mitochondrial aconit...   303   7e-83
BC102642_1(BC102642|pid:none) Bos taurus aconitase 2, mitochondr...   303   7e-83
(P20004) RecName: Full=Aconitate hydratase, mitochondrial;      ...   303   7e-83
protein update 2009. 7.18
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHO133 is nuc
5' end seq. ID VHO133F
5' end seq. 
>VHO133F.Seq
ATTTTTTTTTATATTATATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTGGATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAANATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGNGGNGAAAAA
GATTTANAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGNGNN
NNNNNNNN
Length of 5' end seq. 488
3' end seq. ID VHO133Z
3' end seq. 
>VHO133Z.Seq
NNNNNNNNNNACCAACCACCATCACCAAATGGTCAAAACATTAACGTTATCGTTGATTCA
GAAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAGATTTAGTC
GATATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCGATCACATCTCTATGGCT
GGTCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCAACAATATGTTAATTGGT
GCTATCAACTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATCAATTCACTGGTGAAATC
GGTCCAGTCCCAACCGTCGCTCGTGACTACAAGAAACGTGGTGTCAATTGGATCGTCGTT
GGTGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTGCCCTCGAACCACGTCAT
CTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCCACGAAACCAATCTCAAG
AAACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATTACGATAAAATCTCTGGT
GACGATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAGGTAAACAACTCACTCTC
ATTGTTAAATCTGCTAAACAAGGTTCTGAATTTGAAATTAAAGCTAATCACACCATGAAT
GCGGTCAAATCGAATGGTTCAAAGCTGGTTCCGCTTTAAATTATATTAAATCAGAAAAAG
CCAAAAAAAATT
Length of 3' end seq. 732
Connected seq. ID VHO133P
Connected seq. 
>VHO133P.Seq
ATTTTTTTTTATATTATATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTGGATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAANATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGNGGNGAAAAA
GATTTANAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGNG--
--------ACCAACCACCATCACCAAATGGTCAAAACATTAACGTTATCGTTGATTCAGA
AAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAGATTTAGTCGA
TATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCGATCACATCTCTATGGCTGG
TCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCAACAATATGTTAATTGGTGC
TATCAACTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATCAATTCACTGGTGAAATCGG
TCCAGTCCCAACCGTCGCTCGTGACTACAAGAAACGTGGTGTCAATTGGATCGTCGTTGG
TGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTGCCCTCGAACCACGTCATCT
CGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCCACGAAACCAATCTCAAGAA
ACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATTACGATAAAATCTCTGGTGA
CGATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAGGTAAACAACTCACTCTCAT
TGTTAAATCTGCTAAACAAGGTTCTGAATTTGAAATTAAAGCTAATCACACCATGAATGC
GGTCAAATCGAATGGTTCAAAGCTGGTTCCGCTTTAAATTATATTAAATCAGAAAAAGCC
AAAAAAAATT
Length of connected seq. 1200
Full length Seq ID -
Full length Seq. -
Length of full length seq. -