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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN820
Library VH
(Link to library)
Clone ID VHN820
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11840-1|Contig-U15497-1
Original site URL
Representative seq. ID VHN820P
(Link to Original site)
Representative DNA sequence 
>VHN820 (VHN820Q) /CSM/VH/VHN8-A/VHN820Q.Seq.d/
TTTAAAAAAAAGAAATAAGTATAAAAACAAGATTATATTTTACATTATTATGTGCAGTAG
TATTTTTAGCATGCCAAGGTTATGAATATTTTTTTGCACCATTTAGTATGAATGACGGAA
TATATGGATCATTATTTTTCTTATTAACAGGATTTCATGGATTTCATGTATTAGTAGGAA
GTATATTAATAGGAATAATCACAATAAGATTTATAGTAGGAAATTTCAATCTATTAAATG
TAGGTACAAATTTTCAAATCTATAAAAATAAATCAACAGGATTTGCATGTACATTATTTT
ATTGGCATTTCGTTGATATTGTTTGGATTTTTCTATATATTGTAATATATTGGTGGGGGA
AGTCGATAAAAATTAAACAGTATTATTAACTGATATGTTAATAACTAAAAACAATAAGAA
GAAAATTAAAAAAATCTCGAAAAGAGGAACTAATAAAGCCTTCTTACAAAAATTGTTAAG
TGTTGAAAAATTAACAAACCTTTTAAAAAAAAAACAAAGGAAAGTAAGTTTAGCAAGAAA
AGCNCAAAAAAAGATATATCATAAAGGTAAATTTAAAATAACAXXXXXXXXXXGGTTACA
AAGCACTTGGTAAAGCTACTTCTGAAATTTTAGGTTCATTAACTCCAGTTGCAACTTGTG
GTACTCTTCCTTTGGTTCGTGATCTCCAAGATAGTGGTTTCGATATTCAAATTACTGGTT
TTGGTAAAGAAGAAACTTATCATGCTGATAATGAATATGCTTTATTATCTGATTTTAAAA
ATGCTATTAAAATTTTATCTAATTTTTATAGCAGAATTTAACAACAACAAAATGTCAGAT
AACGAAGCTTTAGATGTCGAAGACTACGCCCAAGCCGGTTCAGGTGCTTCATTAACCTTC
CCAATTCAATGTTCAGCATTAAGAAAGAACGGTTTCGTCGTCATTAAAGGTTTCCCATGT
AAGATTGTTGATATGTCAACTTCCAAAACCGGTAAACACGGTCACGCCAAAGTTAACATC
ACTGCTATCGATATCTTCACTGGTAAGAAATACGAAGAAATTTGCCCATCAACTCACAAC
ATTGATGTACCAAATGTCAGCAGAAAGGAATACACCGTTATGGATGTTCAAGATGGTTAC
TTATCACTCTTGGATGCTGGTGGTGAAGTCAAAGAAGATCTTGCCCTCCCAGAAGATGAT
ATTGGTAAAGAAATTACCCAAATGTTAAAAGAAGGTAAAGAGCCATTAGTTTCAGTCATC
TCTGCTTTAGGTAAAGAAGGTGTCGTCTCTGTTAAAGTCAGCAACAATTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
lkkrnkyknkiifyiimcssifsmprl*iffcti*ye*rniwiiiflinriswiscisrk
yinrnnhnkiysrkfqsikcrykfsnl*k*inricmyiillafr*ycldfsiycnilvge
vdkn*TVLLTDMLITKNNKKKIKKISKRGTNKAFLQKLLSVEKLTNLLKKKQRKVSLARK
AQKKIYHKGKFKIT---

---GYKALGKATSEILGSLTPVATCGTLPLVRDLQDSGFDIQITGFGKEETYHADNEYAL
LSDFKNAIKILSNFYSRI*qqqnvr*rsfrcrrlrpsrfrcfinlpnsmfsikkerfrrh
*rfpm*dc*yvnfqnr*trsrqs*hhcyrylhw*eirrnlpinsqh*ctkcqqkgihryg
csrwllitlgcww*sqrrscpprr*yw*rnypnvkrr*raisfshlcfr*rrcrlc*sqq
ql
Translated Amino Acid sequence (All Frames) 
Frame A:
fkkkk*v*kqdyilhyyvq*yf*hakvmniflhhlv*mteymdhyfsy*qdfmdfmy**e
vy**e*sq*dl**eisiy*m*vqifksikinqqdlhvhyfigislilfgffyil*yiggg
sr*klnsiin*yvnn*kq*een*knlekrn**slltkivkc*kinkpfkkktkeskfskk
sxkkdis*r*i*nn---

---GYKALGKATSEILGSLTPVATCGTLPLVRDLQDSGFDIQITGFGKEETYHADNEYAL
LSDFKNAIKILSNFYSRI*qqqnvr*rsfrcrrlrpsrfrcfinlpnsmfsikkerfrrh
*rfpm*dc*yvnfqnr*trsrqs*hhcyrylhw*eirrnlpinsqh*ctkcqqkgihryg
csrwllitlgcww*sqrrscpprr*yw*rnypnvkrr*raisfshlcfr*rrcrlc*sqq
ql

Frame B:
lkkrnkyknkiifyiimcssifsmprl*iffcti*ye*rniwiiiflinriswiscisrk
yinrnnhnkiysrkfqsikcrykfsnl*k*inricmyiillafr*ycldfsiycnilvge
vdkn*TVLLTDMLITKNNKKKIKKISKRGTNKAFLQKLLSVEKLTNLLKKKQRKVSLARK
AQKKIYHKGKFKIT---

---vtkhlvklllkf*vh*lqlqlvvlflwfviskivvsifkllvlvkkklimlimnmly
ylilkmllkfylifiaefnnnkmsdnealdvedyaqagsgasltfpiqcsalrkngfvvi
kgfpckivdmstsktgkhghakvnitaidiftgkkyeeicpsthnidvpnvsrkeytvmd
vqdgylslldaggevkedlalpeddigkeitqmlkegkeplvsvisalgkegvvsvkvsn
n*

Frame C:
*kkeisiktrlyftllcavvflacqgyeyffapfsmndgiygslfflltgfhgfhvlvgs
iligiitirfivgnfnllnvgtnfqiyknkstgfactlfywhfvdivwiflyiviywwgk
sikikqyy*lic**lktirrklkksrkeelikpsyknc*vlkn*qtf*kknkgk*v*qek
xkkryiikvnlk*---

---lqstw*syf*nfrfinsscnlwyssfgs*spr*wfrysnywfw*rrnlsc***icfi
i*f*kcy*nfi*fl*qnltttkcqitkl*mskttpkpvqvlh*psqfnvqh*ertvsssl
kvshvrllicqlpkpvntvtpkltsllsisslvrntkkfahqlttlmyqmsaerntplwm
fkmvtyhswmlvvkskkilpsqkmilvkklpkc*kkvksh*fqssll*vkkvssllksat
ik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN820 (VHN820Q) /CSM/VH/VHN8-A/VHN820Q.Seq.d/                       2329   0.0
VHD324 (VHD324Q) /CSM/VH/VHD3-A/VHD324Q.Seq.d/                       1017   0.0
AHC103 (AHC103Q) /CSM/AH/AHC1-A/AHC103Q.Seq.d/                       1003   0.0
SFB873 (SFB873Q) /CSM/SF/SFB8-D/SFB873Q.Seq.d/                       1001   0.0
VSG552 (VSG552Q) /CSM/VS/VSG5-C/VSG552Q.Seq.d/                        995   0.0
VSE527 (VSE527Q) /CSM/VS/VSE5-B/VSE527Q.Seq.d/                        989   0.0
SLE525 (SLE525Q) /CSM/SL/SLE5-B/SLE525Q.Seq.d/                        987   0.0
FC-AH24 (FC-AH24Q) /CSM/FC/FC-AH/FC-AH24Q.Seq.d/                      987   0.0
SSD486 (SSD486Q) /CSM/SS/SSD4-D/SSD486Q.Seq.d/                        985   0.0
SSC438 (SSC438Q) /CSM/SS/SSC4-B/SSC438Q.Seq.d/                        985   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ444992) Dictyostelium discoideum cDNA clone:ddv58h06, 3' ...  1421   0.0    1
(BJ419790) Dictyostelium discoideum cDNA clone:ddv37p05, 5' ...  1017   0.0    1
(BJ331364) Dictyostelium discoideum cDNA clone:dda35e01, 5' ...  1003   0.0    1
(BJ387759) Dictyostelium discoideum cDNA clone:dds4b20, 5' e...  1001   0.0    1
(AU266617) Dictyostelium discoideum vegetative cDNA clone:VS...   995   0.0    1
(AU263170) Dictyostelium discoideum vegetative cDNA clone:VS...   989   0.0    1
(AU284284) Dictyostelium discoideum gamete cDNA clone:FC-AH2...   987   0.0    1
(AU034805) Dictyostelium discoideum slug cDNA, clone SLE525.      987   0.0    1
(AU051940) Dictyostelium discoideum slug cDNA, clone SSC438.      985   0.0    1
(C92101) Dictyostelium discoideum slug cDNA, clone SSD486.        985   0.0    1
dna update 2008.12. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P10160) RecName: Full=Eukaryotic translation initiation factor ...   204   4e-51
(P63241) RecName: Full=Eukaryotic translation initiation factor ...   203   1e-50
AY129319_1(AY129319|pid:none) Homo sapiens eukaryotic initiation...   203   1e-50
BC070048_1(BC070048|pid:none) Homo sapiens eukaryotic translatio...   201   4e-50
(Q6IS14) RecName: Full=Eukaryotic translation initiation factor ...   201   4e-50
AL596185_11(AL596185|pid:none) Mouse DNA sequence from clone RP2...   201   5e-50
S72024_1(S72024|pid:none) eif-5A=eukaryotic initiation factor 5A...   201   5e-50
BT075853_1(BT075853|pid:none) Caligus rogercresseyi clone crog-e...   200   8e-50
(Q07460) RecName: Full=Eukaryotic translation initiation factor ...   197   7e-49
EU916931_1(EU916931|pid:none) Ctenopharyngodon idella eukaryotic...   197   9e-49
protein update 2009. 5.17
PSORT 

 psg: 0.23  gvh: 0.28  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.10
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VHN820 is nuc
5' end seq. ID VHN820F
5' end seq. 
>VHN820F.Seq
TTTAAAAAAAAGAAATAAGTATAAAAACAAGATTATATTTTACATTATTATGTGCAGTAG
TATTTTTAGCATGCCAAGGTTATGAATATTTTTTTGCACCATTTAGTATGAATGACGGAA
TATATGGATCATTATTTTTCTTATTAACAGGATTTCATGGATTTCATGTATTAGTAGGAA
GTATATTAATAGGAATAATCACAATAAGATTTATAGTAGGAAATTTCAATCTATTAAATG
TAGGTACAAATTTTCAAATCTATAAAAATAAATCAACAGGATTTGCATGTACATTATTTT
ATTGGCATTTCGTTGATATTGTTTGGATTTTTCTATATATTGTAATATATTGGTGGGGGA
AGTCGATAAAAATTAAACAGTATTATTAACTGATATGTTAATAACTAAAAACAATAAGAA
GAAAATTAAAAAAATCTCGAAAAGAGGAACTAATAAAGCCTTCTTACAAAAATTGTTAAG
TGTTGAAAAATTAACAAACCTTTTAAAAAAAAAACAAAGGAAAGTAAGTTTAGCAAGAAA
AGCNCAAAAAAAGATATATCATAAAGGTAAATTTAAAATAACANNNNNNNNNN
Length of 5' end seq. 593
3' end seq. ID VHN820Z
3' end seq. 
>VHN820Z.Seq
NNNNNNNNNNGGTTACAAAGCACTTGGTAAAGCTACTTCTGAAATTTTAGGTTCATTAAC
TCCAGTTGCAACTTGTGGTACTCTTCCTTTGGTTCGTGATCTCCAAGATAGTGGTTTCGA
TATTCAAATTACTGGTTTTGGTAAAGAAGAAACTTATCATGCTGATAATGAATATGCTTT
ATTATCTGATTTTAAAAATGCTATTAAAATTTTATCTAATTTTTATAGCAGAATTTAACA
ACAACAAAATGTCAGATAACGAAGCTTTAGATGTCGAAGACTACGCCCAAGCCGGTTCAG
GTGCTTCATTAACCTTCCCAATTCAATGTTCAGCATTAAGAAAGAACGGTTTCGTCGTCA
TTAAAGGTTTCCCATGTAAGATTGTTGATATGTCAACTTCCAAAACCGGTAAACACGGTC
ACGCCAAAGTTAACATCACTGCTATCGATATCTTCACTGGTAAGAAATACGAAGAAATTT
GCCCATCAACTCACAACATTGATGTACCAAATGTCAGCAGAAAGGAATACACCGTTATGG
ATGTTCAAGATGGTTACTTATCACTCTTGGATGCTGGTGGTGAAGTCAAAGAAGATCTTG
CCCTCCCAGAAGATGATATTGGTAAAGAAATTACCCAAATGTTAAAAGAAGGTAAAGAGC
CATTAGTTTCAGTCATCTCTGCTTTAGGTAAAGAAGGTGTCGTCTCTGTTAAAGTCAGCA
ACAATTAAA
Length of 3' end seq. 729
Connected seq. ID VHN820P
Connected seq. 
>VHN820P.Seq
TTTAAAAAAAAGAAATAAGTATAAAAACAAGATTATATTTTACATTATTATGTGCAGTAG
TATTTTTAGCATGCCAAGGTTATGAATATTTTTTTGCACCATTTAGTATGAATGACGGAA
TATATGGATCATTATTTTTCTTATTAACAGGATTTCATGGATTTCATGTATTAGTAGGAA
GTATATTAATAGGAATAATCACAATAAGATTTATAGTAGGAAATTTCAATCTATTAAATG
TAGGTACAAATTTTCAAATCTATAAAAATAAATCAACAGGATTTGCATGTACATTATTTT
ATTGGCATTTCGTTGATATTGTTTGGATTTTTCTATATATTGTAATATATTGGTGGGGGA
AGTCGATAAAAATTAAACAGTATTATTAACTGATATGTTAATAACTAAAAACAATAAGAA
GAAAATTAAAAAAATCTCGAAAAGAGGAACTAATAAAGCCTTCTTACAAAAATTGTTAAG
TGTTGAAAAATTAACAAACCTTTTAAAAAAAAAACAAAGGAAAGTAAGTTTAGCAAGAAA
AGCNCAAAAAAAGATATATCATAAAGGTAAATTTAAAATAACA----------GGTTACA
AAGCACTTGGTAAAGCTACTTCTGAAATTTTAGGTTCATTAACTCCAGTTGCAACTTGTG
GTACTCTTCCTTTGGTTCGTGATCTCCAAGATAGTGGTTTCGATATTCAAATTACTGGTT
TTGGTAAAGAAGAAACTTATCATGCTGATAATGAATATGCTTTATTATCTGATTTTAAAA
ATGCTATTAAAATTTTATCTAATTTTTATAGCAGAATTTAACAACAACAAAATGTCAGAT
AACGAAGCTTTAGATGTCGAAGACTACGCCCAAGCCGGTTCAGGTGCTTCATTAACCTTC
CCAATTCAATGTTCAGCATTAAGAAAGAACGGTTTCGTCGTCATTAAAGGTTTCCCATGT
AAGATTGTTGATATGTCAACTTCCAAAACCGGTAAACACGGTCACGCCAAAGTTAACATC
ACTGCTATCGATATCTTCACTGGTAAGAAATACGAAGAAATTTGCCCATCAACTCACAAC
ATTGATGTACCAAATGTCAGCAGAAAGGAATACACCGTTATGGATGTTCAAGATGGTTAC
TTATCACTCTTGGATGCTGGTGGTGAAGTCAAAGAAGATCTTGCCCTCCCAGAAGATGAT
ATTGGTAAAGAAATTACCCAAATGTTAAAAGAAGGTAAAGAGCCATTAGTTTCAGTCATC
TCTGCTTTAGGTAAAGAAGGTGTCGTCTCTGTTAAAGTCAGCAACAATTAAA
Length of connected seq. 1302
Full length Seq ID -
Full length Seq. -
Length of full length seq. -