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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO696
Library AF
(Link to library)
Clone ID AFO696
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID AFO696E
(Link to Original site)
Representative DNA sequence 
>AFO696 (AFO696Q) /CSM/AF/AFO6-D/AFO696Q.Seq.d/
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGC
CATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAA
GATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGT
GAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCT
GGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTC
GATACTATTGCCNCTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAN
AAAGCTCAAACCGTCGTTGATTCTCTCCCANAANAATTAANAACTGAAGGTTCATACTAC
GTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCNCTGAAATCGCT
CGNATCNCCAAATTAGTCTCTGGTTCAATGTCTGGAAANAAAGCTGATGAATTTGCCAAN
AAACNCAATATTTTAGAATCNTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAA
AAATAAAATAAAAGAAAAAAAATAAAATATAAAATAAAATTTTAAAAAAATAAAAAAAAA
AATAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
LLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFVKFYAPWCG
HCKKLAPDFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKIFDKSTTAK
DYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAPWC
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDG
EKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIAXEFIAAAAEVRKELVX
KAQTVVDSLPXXLXTEGSYYVKVMKTIAEKSIDFVTXEIARIXKLVSGSMSGXKADEFAX
KXNILESFKSK*ikfnlenkk*nkrkkikykikf*knkkknk
Translated Amino Acid sequence (All Frames) 
Frame A:
LLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFVKFYAPWCG
HCKKLAPDFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKIFDKSTTAK
DYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVLVEFYAPWC
GHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDG
EKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIAXEFIAAAAEVRKELVX
KAQTVVDSLPXXLXTEGSYYVKVMKTIAEKSIDFVTXEIARIXKLVSGSMSGXKADEFAX
KXNILESFKSK*ikfnlenkk*nkrkkikykikf*knkkknk


Frame B:
y**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttliqllmvqklyl*nsmhhgva
ivknlhqilksllthshqfptkl*lpklivtklttklsvvnmmflviqpsksltnqplpk
ittvldqlmny*lisiitpkptlk*rkhhqmlliyhhqiliqlf*inqrmf*lnsmhhgv
viaks*cqimkslvtptpmkkmlsspkliamllitkpsapnmvslvsqhsngsvnkaktv
knmnkvvililssttstnklvltvskvvnsllvlvvlnnsillplnsslpllksvrnssx
klkpslilsxxn*xlkvhttsks*kpllknqsil*plkslxspn*slvqclexklmnlpx
nxif*nxsnqnklnli*kiknkikekk*nik*nfkkikkki


Frame C:
inntstkneniiicyincigicclmlsrrkcccfitrql*ysc*wfkncickilctmvwp
l*ktctrf*npc*hirtsfqqscncqs*l*ps*qqssl**i*cfwlsnpqnl*qinhcqr
lqrc*is**iinlyq*srqnqr*skestikcc*fitikf*fscfr*ikecfs*ilctmvw
slqkvnarl*npw*hlrq*krcchrqn*lrcc**qshllqiwchwfpntqmvr*tkqrr*
ki*trs*s*yfhqlhqqtswc*pcqrw*trcwcwsc*ttryycx*ihrcrc*sp*gtrqx
ssnrr*fspxxixn*rfilrqshenhc*kinrfcnx*nrsxxqislwfnvwkxs**icqx
tqyfrixqikin*i*frk*kik*kkknki*nkilkk*kkk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       2085   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       2085   0.0
VFL370 (VFL370Q) /CSM/VF/VFL3-C/VFL370Q.Seq.d/                       2085   0.0
VFI769 (VFI769Q) /CSM/VF/VFI7-C/VFI769Q.Seq.d/                       2085   0.0
VFG408 (VFG408Q) /CSM/VF/VFG4-A/VFG408Q.Seq.d/                       2085   0.0
VFF762 (VFF762Q) /CSM/VF/VFF7-C/VFF762Q.Seq.d/                       2085   0.0
VFF615 (VFF615Q) /CSM/VF/VFF6-A/VFF615Q.Seq.d/                       2085   0.0
VFD309 (VFD309Q) /CSM/VF/VFD3-A/VFD309Q.Seq.d/                       2085   0.0
SFK776 (SFK776Q) /CSM/SF/SFK7-D/SFK776Q.Seq.d/                       2085   0.0
SFI534 (SFI534Q) /CSM/SF/SFI5-B/SFI534Q.Seq.d/                       2085   0.0
own update 2001.12. 2
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1824  0.0  3
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1112  0.0  8
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.     54  3e-19  4
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    58  3e-19  3
AU299541|AU299541.1 Cryptomeria japonica mRNA, clone: CM1065, 5' end, expressed in male cone.    62  3e-13  2
CB090389|CB090389.1 gy74e10.g1 Cycad Leaf Library (NYBG) Cycas rumphii cDNA clone gy74e10, mRNA sequence.    80  1e-12  2
CF508720|CF508720.1 USDA-FP_124000-223 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-70_B02 5', mRNA sequence.    68  4e-12  2
CF509886|CF509886.1 USDA-FP_125000-394 Immature Ovaries from field-collected Valencia Sweet Orange (Citrus sinensis (L.) Osbeck) Citrus sinensis cDNA clone MVF-82_F01 5', mRNA sequence.    68  4e-12  2
BE208912|BE208912.1 GF-FV-P3F1 Marsh grapefruit young flavedo Citrus x paradisi cDNA, mRNA sequence.    68  4e-12  2
CD576151|CD576151.1 UCRPT01_03ce04_g3 Poncirus trifoliata CTV-challenged cDNA library - AGI Poncirus trifoliata cDNA clone UCRPT01_03ce04, mRNA sequence.    68  5e-12  2
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   678   0.0
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   676   0.0
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   314   3e-84
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   306   1e-81
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   300   7e-80
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   299   1e-79
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   299   1e-79
T03644(T03644) probable protein disulfide-isomerase (EC 5.3.4.1)...   299   2e-79
AM485099_1(AM485099|pid:none) Vitis vinifera contig VV78X160692....   296   1e-78
AY739288_1(AY739288|pid:none) Zea mays protein disulfide isomera...   295   2e-78
protein update 2009. 6.29
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.45  top: 0.10  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial

>> prediction for AFO696 is exc
5' end seq. ID AFO696F
5' end seq. 
>AFO696F.Seq
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGC
CATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAA
GATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAA----------
Length of 5' end seq. 693
3' end seq. ID AFO696Z
3' end seq. 
>AFO696Z.Seq
----------TGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCT
ACGCCAATGAAAAANATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAG
CCATNTGCTCCAAANATGGTGTCNCTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAA
GCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCA
ACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTG
TTGAACAACTCGATACTATTGCCNCTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGG
AACTCGTCAANAAAGCTCAAACCGTCGTTGATTCTCTCCCANAANAATTAANAACTGAAG
GTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCN
CTGAAATCGCTCGNATCNCCAAATTAGTCTCTGGTTCAATGTCTGGAAANAAAGCTGATG
AATTTGCCAANAAACNCAATATTTTAGAATCNTTCAAATCAAAATAAATTAAATTTAATT
TAGAAAATAAAAAATAAAATAAAAGAAAAAAAATAAAATATAAAATAAAATTTTAAAAAA
ATAAAAAAAAAAATAAA
Length of 3' end seq. 667
Connected seq. ID AFO696P
Connected seq. 
>AFO696P.Seq
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGC
CATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAA
GATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAA----------TGGTCATTGCAAAAAGT
TAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAAAAANATGTTGTCATCG
CCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATNTGCTCCAAANATGGTGTCNCTG
GTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAG
GTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCA
AAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCNCTG
AATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAANAAAGCTCAAACCGTCG
TTGATTCTCTCCCANAANAATTAANAACTGAAGGTTCATACTACGTCAAAGTCATGAAAA
CCATTGCTGAAAAATCAATCGATTTTGTAACCNCTGAAATCGCTCGNATCNCCAAATTAG
TCTCTGGTTCAATGTCTGGAAANAAAGCTGATGAATTTGCCAANAAACNCAATATTTTAG
AATCNTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAA
AAAAAATAAAATATAAAATAAAATTTTAAAAAAATAAAAAAAAAAATAAA
Length of connected seq. 1360
Full length Seq ID AFO696E
Full length Seq. 
>AFO696E.Seq
TTATTAATAATACATCCACAAAGAATGAAAATATTATTATTTGTTACATTAATTGCATTG
GCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTGTTGTTTTATCACCAGACAACTTT
GATACAGTTGTTGATGGTTCAAAAACTGTATTTGTAAAATTCTATGCACCATGGTGTGGC
CATTGTAAAAAACTTGCACCAGATTTTGAAATCCTTGCTGACACATTCGCACCAGTTTCC
AACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAAGCTGACAACAAAGCTCTCTGTAGT
AAATATGATGTTTCTGGTTATCCAACCCTCAAAATCTTTGACAAATCAACCACTGCCAAA
GATTACAACGGTGCTAGATCAGTTGATGAATTATTAACTTATATCAATAATCACGCCAAA
ACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTTGTTGATTTATCACCATCAAATTTT
GATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTAGTTGAATTCTATGCACCATGGTGT
GGTCATTGCAAAAAGTTAATGCCAGATTATGAAATCCTTGGTAACACCTACGCCAATGAA
AAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAGCCATCTGCTCC
AAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAAGCAAAGACGGT
GAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCAACAAACAAGCT
GGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTGTTGAACAACTC
GATACTATTGCCNCTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGGAACTCGTCAAN
AAAGCTCAAACCGTCGTTGATTCTCTCCCANAANAATTAANAACTGAAGGTTCATACTAC
GTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCNCTGAAATCGCT
CGNATCNCCAAATTAGTCTCTGGTTCAATGTCTGGAAANAAAGCTGATGAATTTGCCAAN
AAACNCAATATTTTAGAATCNTTCAAATCAAAATAAATTAAATTTAATTTAGAAAATAAA
AAATAAAATAAAAGAAAAAAAATAAAATATAAAATAAAATTTTAAAAAAATAAAAAAAAA
AATAAA
Length of full length seq. 1206