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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN654
Library VH
(Link to library)
Clone ID VHN654
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16543-1
Original site URL
Representative seq. ID VHN654P
(Link to Original site)
Representative DNA sequence 
>VHN654 (VHN654Q) /CSM/VH/VHN6-C/VHN654Q.Seq.d/
ATNAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGA
ATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGG
TCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTAC
ATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAG
TGTTCCTACATTCTATTTTTACATAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAA
TGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAAT
TGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTATTT
TATTTAATTTATNTTTTAAAAAAAAAAXXXXXXXXXXATCAAATAAATAATTTATATTAA
AAAATGTCCANAGTAATTCATATTTCATTTAATGAANAATTAGATAAACNTTTGCAAGNT
GAAANATTAGTGATTGACTTTAGTGCTGCATGGNGTGGTCCATGTANAGCAANATTTCCG
GTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTACATTNGTTCATGTTGACATTGAT
AAACTAAGNGGTCATCCAATAGTAAAANAAATTANAAGTGTTCCTACATTNTATTTTTAC
ATAAATGGAGNTAAAGTATCANAATTCAGTGGNGCCAATGAAGCAACTNTAANAAGTACT
NTAAAAGCAAACNAATAATTTTTTGTTAAATTAATAATTGGACAAAAAATGCNACNAATT
AAATAAATAAAAAAAAAAAAATATTCACAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
xnk*filknvqsnsyfi**rir*tfas*kisd*l*ccmvwsm*snisgi*kafkricniy
irsc*h**tkwssnskrn*kcsyilflhkws*siriqwcq*snskkysrskqiifc*inn
ctknatn*INKKKKIIXKLFYLIYXLKKK---

---QINNLY*kmsxvihisfnexldkxlqxexlvidfsaawxgpcxaxfpvfeklsnefv
tftxvhvdidklxghpivkxixsvptxyfyingxkvsxfsganeatxxstxkanx*ffvk
liigqkmxxik*ikkkkysqi
Translated Amino Acid sequence (All Frames) 
Frame A:
xnk*filknvqsnsyfi**rir*tfas*kisd*l*ccmvwsm*snisgi*kafkricniy
irsc*h**tkwssnskrn*kcsyilflhkws*siriqwcq*snskkysrskqiifc*inn
ctknatn*INKKKKIIXKLFYLIYXLKKK---

---ik*iiyikkcpx*fifhlmxn*inxckxkx**ltlvlhgvvhvxqxfrylksfqtnl
*hlhxfmltlin*xviq**xklxvflhxift*mexkyxnsvxpmkql*xvl*kqtnnfll
n**ldkkcxxlnk*kkknihk

Frame B:
xinnly*kmsrvihissneeldkhlqaerlvidfsaawcgpcraispvfeklsnefvtft
fvhvdidklsghpivkeirsvptfyfyingakvsefsganeatlrstleank*ffvklii
vqkmqqik*ikkkk*fxnyfi*fxf*kk---

---snk*filknvxsnsyfi**xir*tfax*xisd*l*ccmxwsmxsxisgi*kafkric
niyixsc*h**tkxssnskxnxkcsyixflhkwx*sixiqwxq*snxxkyxkskxiifc*
innwtknatn*inkkkkiftn

Frame C:
x*iiyikkcpe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksfqtnl*hlh
sfmltlin*vviq**kklevflhsift*melkyqnsvvpmkql*evl*kqtnnflln**l
ykkcnklnk*kkknnsqiilfnlxfkkk---

---QINNLY*kmsxvihisfnexldkxlqxexlvidfsaawxgpcxaxfpvfeklsnefv
tftxvhvdidklxghpivkxixsvptxyfyingxkvsxfsganeatxxstxkanx*ffvk
liigqkmxxik*ikkkkysqi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN654 (VHN654Q) /CSM/VH/VHN6-C/VHN654Q.Seq.d/                        767   0.0
VSJ416 (VSJ416Q) /CSM/VS/VSJ4-A/VSJ416Q.Seq.d/                        759   0.0
VSI646 (VSI646Q) /CSM/VS/VSI6-B/VSI646Q.Seq.d/                        759   0.0
VSD821 (VSD821Q) /CSM/VS/VSD8-A/VSD821Q.Seq.d/                        759   0.0
VSD665 (VSD665Q) /CSM/VS/VSD6-C/VSD665Q.Seq.d/                        759   0.0
VSD645 (VSD645Q) /CSM/VS/VSD6-B/VSD645Q.Seq.d/                        759   0.0
SSM584 (SSM584Q) /CSM/SS/SSM5-D/SSM584Q.Seq.d/                        759   0.0
SSM341 (SSM341Q) /CSM/SS/SSM3-B/SSM341Q.Seq.d/                        759   0.0
SSE809 (SSE809Q) /CSM/SS/SSE8-A/SSE809Q.Seq.d/                        759   0.0
SSA493 (SSA493Q) /CSM/SS/SSA4-D/SSA493Q.Seq.d/                        759   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ426334) Dictyostelium discoideum cDNA clone:ddv58k14, 5' ...   646   0.0    2
(C93126) Dictyostelium discoideum slug cDNA, clone SSM584.        638   0.0    2
(AU261936) Dictyostelium discoideum vegetative cDNA clone:VS...   638   0.0    2
(AU264360) Dictyostelium discoideum vegetative cDNA clone:VS...   638   0.0    2
(C94330) Dictyostelium discoideum slug cDNA, clone SSK844.        630   0.0    2
(AU264394) Dictyostelium discoideum vegetative cDNA clone:VS...   638   0.0    2
(AU262747) Dictyostelium discoideum vegetative cDNA clone:VS...   632   0.0    2
(C90369) Dictyostelium discoideum slug cDNA, clone SSI316.        624   0.0    2
(C92281) Dictyostelium discoideum slug cDNA, clone SSD338.        624   0.0    2
(AU261698) Dictyostelium discoideum vegetative cDNA clone:VS...   630   0.0    2
dna update 2008.12. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P29447) RecName: Full=Thioredoxin-3;          Short=Trx-3;           187   4e-46
C46264(C46264) thioredoxin 3 - slime mold (Dictyostelium discoid...   184   2e-45
(P29446) RecName: Full=Thioredoxin-2;          Short=Trx-2; Flag...   179   1e-43
BC129794_1(BC129794|pid:none) Xenopus laevis hypothetical protei...    92   3e-17
BC166957_1(BC166957|pid:none) Xenopus tropicalis hypothetical pr...    91   6e-17
BC072884_1(BC072884|pid:none) Xenopus laevis MGC80314 protein, m...    89   2e-16
(Q9DGI3) RecName: Full=Thioredoxin;          Short=Trx;  &AF2936...    85   3e-15
FJ384985_1(FJ384985|pid:none) Bombina orientalis thioredoxin mRN...    83   1e-14
DQ215312_1(DQ215312|pid:none) Taeniopygia guttata clone 0058P004...    83   1e-14
(P08629) RecName: Full=Thioredoxin;          Short=Trx;  &A30006...    83   1e-14
protein update 2009. 5.17
PSORT 

 psg: 0.90  gvh: 0.29  alm: 0.52  top: 0.47  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.23  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHN654 is nuc
5' end seq. ID VHN654F
5' end seq. 
>VHN654F.Seq
ATNAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGA
ATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGG
TCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTAC
ATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAG
TGTTCCTACATTCTATTTTTACATAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAA
TGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAAT
TGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTATTT
TATTTAATTTATNTTTTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 457
3' end seq. ID VHN654Z
3' end seq. 
>VHN654Z.Seq
NNNNNNNNNNATCAAATAAATAATTTATATTAAAAAATGTCCANAGTAATTCATATTTCA
TTTAATGAANAATTAGATAAACNTTTGCAAGNTGAAANATTAGTGATTGACTTTAGTGCT
GCATGGNGTGGTCCATGTANAGCAANATTTCCGGTATTTGAAAAGCTTTCAAACGAATTT
GTAACATTTACATTNGTTCATGTTGACATTGATAAACTAAGNGGTCATCCAATAGTAAAA
NAAATTANAAGTGTTCCTACATTNTATTTTTACATAAATGGAGNTAAAGTATCANAATTC
AGTGGNGCCAATGAAGCAACTNTAANAAGTACTNTAAAAGCAAACNAATAATTTTTTGTT
AAATTAATAATTGGACAAAAAATGCNACNAATTAAATAAATAAAAAAAAAAAAATATTCA
CAAATA
Length of 3' end seq. 426
Connected seq. ID VHN654P
Connected seq. 
>VHN654P.Seq
ATNAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGA
ATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGG
TCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTAC
ATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAG
TGTTCCTACATTCTATTTTTACATAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAA
TGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAAT
TGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTATTT
TATTTAATTTATNTTTTAAAAAAAAAA----------ATCAAATAAATAATTTATATTAA
AAAATGTCCANAGTAATTCATATTTCATTTAATGAANAATTAGATAAACNTTTGCAAGNT
GAAANATTAGTGATTGACTTTAGTGCTGCATGGNGTGGTCCATGTANAGCAANATTTCCG
GTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTACATTNGTTCATGTTGACATTGAT
AAACTAAGNGGTCATCCAATAGTAAAANAAATTANAAGTGTTCCTACATTNTATTTTTAC
ATAAATGGAGNTAAAGTATCANAATTCAGTGGNGCCAATGAAGCAACTNTAANAAGTACT
NTAAAAGCAAACNAATAATTTTTTGTTAAATTAATAATTGGACAAAAAATGCNACNAATT
AAATAAATAAAAAAAAAAAAATATTCACAAATA
Length of connected seq. 863
Full length Seq ID -
Full length Seq. -
Length of full length seq. -